TLR9 Antibody (26C593.2) - Azide Free

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Primate (Rhesus monkey).

We have publications tested in 6 applications: Flow Cytometry Control, IF/IHC, IHC-Fr, IHC-P, In vitro, WB.

Showing Publications 1 - 10 of 55. Show All 55 Publications. Collapse Publications.

Publications using NBP2-24863	Applications	Species
Parviainen E The effect of human papillomavirus oncoprotein E6 on the expression of Toll-like receptor 9 in breast cancer Thesis 2022-01-01 (WB, Human)	WB	Human
Sipola S. COLECTOMY IN AN ICU PATIENT POPULATION Thesis 2014-01-01 (IHC-P, Human) Details: Normal and diseased colon, the TLR9 mAb was used at 1:150.	IHC-P	Human
Guerrier T, Pochard P, Lahiri A et al. TLR9 expressed on plasma membrane acts as a negative regulator of human B cell response. J. Autoimmun. 2014-02-25 [PMID: 24582318] (In vitro, Human) Details: Human B cells: The TLR9 mAb was used to stimulate cells, Figs 4, 5.	In vitro	Human
Belmont L, Rabbe N, Antoine M et al. Expression of TLR9 in tumor-infiltrating mononuclear cells enhances angiogenesis and is associated with a worse survival in lung cancer. Int J Cancer. 2013-08-03 [PMID: 23913633]
Abdi J, Mutis T, Garssen J, Redegeld F. Characterization of the Toll-like receptor expression profile in human multiple myeloma cells. PLoS One. 2013-04-08 [PMID: 23593278] Details: Antibodies cited [multiple myeloma (MM) cell lines, human intestine tissue lysate, PBMC, mouse splenocytes] for WB (Figs 2, 3, 4A, 4B, 5, Table 1) and Flow (Intracellular): Figs 4C, 6-8, Table 1: 1. TLR1 (IMG-5012) 2. TLR2 (IMG-416A)3. TLR3 (IMG-315A)4 TL
Siponen M, Kauppila JH, Soini Y, Salo T. TLR4 and TLR9 are induced in oral lichen planus. J Oral Pathol Med. 2012-11-01 [PMID: 22672741]
Faruk EM,Yousef MM, Mohamed T. Does vitamin D have protective effect on human nasal polyposis: histological and immunohistochemical study. Journal of Histology & Histopathology 2014-01-01 (IHC-P, Human) Details: Nasal mucosa tissue from biopsies, Fig 4, Table 5.	IHC-P	Human
Figueiredo MM, Amorim IF, Pinto AJ et al. Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum. BMC Immunol. 2013-05-14 [PMID: 23668673]
Karim R, Meyers C, Backendorf C et al. Human papillomavirus deregulates the response of a cellular network comprising of chemotactic and proinflammatory genes. PLoS One. 2011-03-14 [PMID: 21423754]
Dasgupta G, Chentoufi AA, You S et al. Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses. Invest Ophthalmol Vis Sci. 2011-05-17 [PMID: 21273544] Details: Products cited: Flow (intracellular), Fig 1A ( CD4+CD25+ regulatory T cells purified from rabbit conjunctiva) & Fig 2A (CD11b+ cells isolated from rabbit conjunctiva): TLR2/CD282 PE (IMG-416D), TLR3/CD283 (IMG-315D), TLR4/CD284 (IMG-417D), TLR8/CD288 (IMG
van Gent M, Griffin BD, Berkhoff EG et al. EBV lytic-phase protein BGLF5 contributes to TLR9 downregulation during productive infection. J Immunol. 2011-02-01 [PMID: 21191071] Details: TLR9/CD289 (IMG-305A). WB: Ak31 and AkBM cell postnuclear lysates infected with Epstein-Barr virus, Fig 2B. WB: TLR9 transfected 293T cells, Fig 4D. Note: The TLR9 antibody (IMG-305A) was transfected validated by WB in 293T cells, Fig 4D.
Myles A, Aggarwal A. Expression of Toll-like receptors 2 and 4 is increased in peripheral blood and synovial fluid monocytes of patients with enthesitis-related arthritis subtype of juvenile idiopathic arthritis. Rheumatology (Oxford). 2011-03-01 [PMID: 21097451]
Schneberger D, Lewis D, Caldwell S, Singh B. Expression of toll-like receptor 9 in lungs of pigs, dogs and cattle. Int J Exp Pathol. 2011-02-01 [PMID: 21044185]
Wang C, Cao S, Yan Y et al. TLR9 expression in glioma tissues correlated to glioma progression and the prognosis of GBM patients. BMC Cancer. 2010-08-10 [PMID: 20696081]
Booth JS, Arsenault R, Napper S et al. TLR9 signaling failure renders Peyer's patch regulatory B cells unresponsive to stimulation with CpG oligodeoxynucleotides. J Innate Immun. 2010-01-01 [PMID: 20551621]
Keestra AM, de Zoete MR, Bouwman LI, van Putten JP. Chicken TLR21 is an innate CpG DNA receptor distinct from mammalian TLR9. J Immunol. 2010-07-01 [PMID: 20498358] Details: Confocal microscopy: HEk293 cells transfacted with hTLR9, Fig 6A. Note: Monoclonal TLR9 antibody was transfected validated in TLR9 transfected HEk293 cells by confocal microscopy, Fig 6A.
Jukkola-Vuorinen A, Rahko E, Vuopala KS et al. Toll-like receptor-9 expression is inversely correlated with estrogen receptor status in breast cancer. J Innate Immun. 2009-01-01 [PMID: 20375566]
Machida H, Ito S, Hirose T et al. Expression of Toll-like receptor 9 in renal podocytes in childhood-onset active and inactive lupus nephritis. Nephrol Dial Transplant. 2010-08-01 [PMID: 20181802] Details: TLR9 (IMG-305A) IHC (paraffin), Fig 2A, human normal kidney control. Fig 2B, Fig 2C, active lupus kidney.
Kim YI, Park JE, Brand DD et al. Protein kinase D1 is essential for the proinflammatory response induced by hypersensitivity pneumonitis-causing thermophilic actinomycetes Saccharopolyspora rectivirgula. J Immunol. 2010-03-15 [PMID: 20142359] Details: TLR9 (IMG-305A) WB, Fig 2, lung cells from MyD88 deficient mice.
Panda A, Qian F, Mohanty S et al. Age-associated decrease in TLR function in primary human dendritic cells predicts influenza vaccine response. J Immunol. 2010-03-01 [PMID: 20100933]
Chentoufi AA, Dasgupta G, Nesburn AB et al. Nasolacrimal duct closure modulates ocular mucosal and systemic CD4(+) T-cell responses induced following topical ocular or intranasal immunization. Clin Vaccine Immunol. 2010-03-01 [PMID: 20089796]
Wu J, Meng Z, Jiang M et al. Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific. Immunology. [PMID: 19922426] Details: Citation using the PE/Cy5 form of this antibody.
Zhou M, McFarland-Mancini MM, Funk HM et al. Toll-like receptor expression in normal ovary and ovarian tumors. Cancer Immunol Immunother. 2009-09-01 [PMID: 19184006]
Palladino Michael A, Savarese Michael A, Chapman Jessica L et al. Localization of Toll-like receptors on epididymal epithelial cells and spermatozoa. Am J Reprod Immunol. 2008-12-01 [PMID: 19032616]
Meneghin A, Choi ES, Evanoff HL et al. TLR9 is expressed in idiopathic interstitial pneumonia and its activation promotes in vitro myofibroblast differentiation. Histochem Cell Biol. 2008-11-01 [PMID: 18633634]
Cohen PA, Koski GK, Czerniecki BJ et al. STAT3- and STAT5-dependent pathways competitively regulate the pan-differentiation of CD34pos cells into tumor-competent dendritic cells. Blood. 2008-09-01 [PMID: 18577706] Details: Flow (intracellular), mouse bone marrow cells, Fig. 1E: 1. TLR3 FITC (IMG-315C) 2. TLR4 FITC (IMG-417C) 3. TLR7 (IMG-665A) 4. TLR8 FITC (IMG-321C) 5. TLR9 FITC (IMG-305C).
Selleri S, Palazzo M, Deola S et al. Induction of pro-inflammatory programs in enteroendocrine cells by the Toll-like receptor agonists flagellin and bacterial LPS. Int Immunol. 2008-08-01 [PMID: 18544573] (Flow Cytometry Control, Human) Details: TLR9-FITC (IMG-305C): Flow (intracellular), Human colon neuroendocrine LCC-18 cell line: Fig. 4d.	Flow Cytometry Control	Human
Ju X, Zenke M, Hart DN, Clark GJ. CD300a/c regulate type I interferon and TNF-alpha secretion by human plasmacytoid dendritic cells stimulated with TLR7 and TLR9 ligands. Blood. 2008-08-15 [PMID: 18535206]
Zhang YW, Davis EG, Blecha F, Wilkerson MJ. Molecular cloning and characterization of equine Toll-like receptor 9. Vet Immunol Immunopathol. 2008-08-15 [PMID: 18462806]
Cognasse F, Hamzeh-Cognasse H, Lafarge S et al. Identification of two subpopulations of purified human blood B cells, CD27- CD23+ and CD27high CD80+, that strongly express cell surface Toll-like receptor 9 and secrete high levels of interleukin-6. Immunology. 2008-11-01 [PMID: 18445007]
Burgener IA, Jungi TW. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008-07-15 [PMID: 18439687] Details: Canine (dog) PBMC subpopulations: 1. 10083K [IC-Flow (Intracellular Staining Flow Assay) Kit.
Nakamura K, Miyazato A, Xiao G et al. Deoxynucleic acids from Cryptococcus neoformans activate myeloid dendritic cells via a TLR9-dependent pathway. J Immunol. 2008-03-15 [PMID: 18322216] Details: TLR9-FITC (IMG-305C) for IF/ICC (intracellular confocal microscopy) in the following figures: 1. Fig 8a: primary mouse bone marrow-derived myeloid dendritic cells (BM-DC) treated with Cn-DNA and CpG-Rhodamine and analyzed over a time course 2. Fig 8b: BM-DC and RAW264.1 cells treated with Cn-DNA Note: intracellular redistribution of TLR9 was observed in treated cells.
Pegu A, Qin S, Fallert Junecko BA et al. Human lymphatic endothelial cells express multiple functional TLRs. J Immunol. 2008-03-01 [PMID: 18292566] Details: Antibodies cited [Flow (Cell surface) and Flow (Intracellular), human lymphatic endothelial cells, Fig. 1D]: 1. IMG-663C (TLR5-FITC) 2. IMG-304C (TLR6-FITC).
Meng Y, Kujas M, Marie Y et al. Expression of TLR9 within human glioblastoma. J Neurooncol. 2008-05-01 [PMID: 18253698] (IHC-P, Human)	IHC-P	Human
Nakano S, Morimoto S, Suzuki J et al. Role of pathogenic auto-antibody production by Toll-like receptor 9 of B cells in active systemic lupus erythematosus. Rheumatology (Oxford). 2008-02-01 [PMID: 18160420] (Flow Cytometry Control)	Flow Cytometry Control
Tran N, Koch A, Berkels R et al. Toll-like receptor 9 expression in murine and human adrenal glands and possible implications during inflammation. J Clin Endocrinol Metab. 2007-07-01 [PMID: 17473064]
Kindrachuk J, Potter JE, Brownlie R et al. Nucleic acids exert a sequence-independent cooperative effect on sequence-dependent activation of Toll-like receptor 9. J Biol Chem. 2007-05-11 [PMID: 17355967] Details: Product cited: TLR9 (IMG-305A) 1. WB, Fig 1: TLR9 stably transfected bovine kidney epithelial cells, these cells secrete recombinant TLR9 (ligand binding domain) into the media. The data shows the molecular weight of TLR9 was reduced following deglycosyla
Wong CK, Cheung PF, Ip WK et al. Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils. Am J Respir Cell Mol Biol. 2007-07-01 [PMID: 17332440] Details: Human blood eosinophils and neutrophils from buffy coat: For WB, Fig. 1A: TLR1 (IMG-5012), TLR5 (IMG-664), TLR6 (IMG-304A), TLR7 (IMG-540), TLR8 (IMG-321A), TLR9 (IMG-305A). For Flow (Intracellular) and Flow (Surface), Fig. 1B: TLR1 (IMG-5021), TLR2 (IMG-416C), TLR3 (IMG-315C), TLR4 (IMG-417C), TLR5 (IMG-663C), TLR6 (IMG-304C), TLR7 (IMG-665A), TLR8 (IMG-321C), TLR9 (IMG-305C).
Ewaschuk JB, Backer JL, Churchill TA et al. Surface expression of Toll-like receptor 9 is upregulated on intestinal epithelial cells in response to pathogenic bacterial DNA. Infect Immun. 2007-05-01 [PMID: 17325049]
Palladino M A, Johnson T A, Gupta R et al. Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tract. Biol Reprod. 2007-06-01 [PMID: 17314314] (WB)	WB
Lee J, Mo JH, Katakura K et al. Maintenance of colonic homeostasis by distinctive apical TLR9 signalling in intestinal epithelial cells. Nat Cell Biol. 2006-12-01 [PMID: 17128265]
Niessner A, Sato K, Chaikof EL et al. Pathogen-sensing plasmacytoid dendritic cells stimulate cytotoxic T-cell function in the atherosclerotic plaque through interferon-alpha. Circulation. 2006-12-05 [PMID: 17116765] (IHC-Fr) Details: TLR9 (IMG-305) [IHC-F, Fig.3A (carotid plaque tissues)].	IHC-Fr
Mansson A, Adner M, Cardell LO. Toll-like receptors in cellular subsets of human tonsil T cells: altered expression during recurrent tonsillitis. Respir Res. 2006-02-27 [PMID: 16504163] Details: Antibodies cited (human tonsils separated into cell subtypes): 1. TLR3 [IMG-315D (Flow-Intracellular), Figs 5 and 6]. 2. TLR5 [IMG-663A (Flow-Intracellular), Fig 6]. 3. TLR9 [IMG-305C (Flow-Intracellular), Fig 4.].
Gottenberg JE, Cagnard N, Lucchesi C et al. Activation of IFN pathways and plasmacytoid dendritic cell recruitment in target organs of primary Sjogren's syndrome. Proc Natl Acad Sci U S A. 2006-02-21 [PMID: 16477017] (IF/IHC)	IF/IHC
Martin-Armas M, Simon-Santamaria J, Pettersen I et al. Toll-like receptor 9 (TLR9) is present in murine liver sinusoidal endothelial cells (LSECs) and mediates the effect of CpG-oligonucleotides. J Hepatol. 2006-05-01 [PMID: 16458386] (IHC-P)	IHC-P
Lee J, Wu CC, Lee KJ et al. Activation of anti-hepatitis C virus responses via Toll-like receptor 7. Proc Natl Acad Sci U S A. 2006-02-07 [PMID: 16446426]
Damiano V, Caputo R, Bianco R et al. Novel toll-like receptor 9 agonist induces epidermal growth factor receptor (EGFR) inhibition and synergistic antitumor activity with EGFR inhibitors. Clin Cancer Res. [PMID: 16428503] Details: Citation using the Azide Free form of this antibody.
Tabeta K, Hoebe K, Janssen EM et al. The Unc93b1 mutation 3d disrupts exogenous antigen presentation and signaling via Toll-like receptors 3, 7 and 9. Nat Immunol. 2006-02-01 [PMID: 16415873] Details: TLR9 (IMG-305) [IF/ICC, Fig.5g-i (primary mouse macrophages)]. The specificity of the TLR9 antibody is demonstrated using primary mouse macrophages from wildtype (5g) and TLR9 knockout (5i) mice.
Srivastava MD, Srivastava BI. Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line. Leuk Res. 2005-07-01 [PMID: 15927677] Details: IF/ICC in SRIK-NKL cells: TLR2 (IMG416), TLR4 (IMG-417), TLR7 (IMG-581), and TLR9 (IMG-305).
Miller LS, SOrensen OE, Liu PT et al. TGF-alpha regulates TLR expression and function on epidermal keratinocytes. J Immunol. 2005-05-15 [PMID: 15879109]
Abel K, Wang Y, Fritts L et al. Deoxycytidyl-deoxyguanosine oligonucleotide classes A, B, and C induce distinct cytokine gene expression patterns in rhesus monkey peripheral blood mononuclear cells and distinct alpha interferon responses in TLR9-expressing rhesus monkey plasmacytoid den Clin Diagn Lab Immunol. 2005-05-01 [PMID: 15879022] (Flow Cytometry Control, Primate (Rhesus monkey)) Details: IMG-305C [Flow (Intracellular), Fig. 5] on Rhesus monkey spleen cell suspensions and PBMC.	Flow Cytometry Control	Primate (Rhesus monkey)
Cognasse F, Hamzeh H, Chavarin P et al. Evidence of Toll-like receptor molecules on human platelets. Immunol Cell Biol. 2005-04-01 [PMID: 15748217] Details: TLR2-PE (IMG-416D), TLR4-PE (IMG-417D), TLR6 (IMG-304A), TLR8-PE (IMG-321D), TLR9-PE (IMG-305D). Applications: Intracellular Flow Cytomety and Cell Surface Flow Cytometry: Figs 1 and 2. A comparison of staining results, intracellular versus cell surface flow cytometry is shown. Cell type: Human platelets.
Greene CM, Carroll TP, Smith SG et al. TLR-induced inflammation in cystic fibrosis and non-cystic fibrosis airway epithelial cells. J Immunol. 2005-02-01 [PMID: 15661927] Details: Functional Assays: 1.TLR2/NF-kB/SEAP Stably Transfected HEK 293 Cells (IML-102). 2. TLR4/MD-2/NF-kB/SEAP Stably Transfected HEK 293 Cells (IML-104MD2). 3. TLR4/NF-kB/SEAP Stably Transfected HEK 293 Cells (IML-104).4.TLR7/NF-kB/SEAP Stably Transfected HEK 293 Cells (IML-107). 5. LPS from E. Coli, TLR4 Ligand (IMG-2204). 6. SEAPorter Assay Kit (10055K).
Droemann D, Albrecht D, Gerdes J et al. Human lung cancer cells express functionally active Toll-like receptor 9. Respir Res. 2005-01-04 [PMID: 15631627] (IF/IHC)	IF/IHC
Lee HK, Dunzendorfer S, Tobias PS. Cytoplasmic domain-mediated dimerizations of toll-like receptor 4 observed by beta-lactamase enzyme fragment complementation. J Biol Chem. 2004-03-12 [PMID: 14699116] (WB) Details: Antibodies cited: TLR9 (IMG-305) [WB, Fig5B (HEK293 cells)].	WB
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• TLR9 Antibodies
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