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TLR9 Antibody (26C593.2) [FITC]

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Flow Cytometry: TLR9 Antibody (26C593.2) [FITC] [NBP2-24729F] - Analysis using the FITC conjugate of NBP2-24729. Staining of TLR9 in Ramos cells using 0.5 ug of this antibody. Green represents isotype control; red ...read more
Flow Cytometry: TLR9 Antibody (26C593.2) [FITC] [NBP2-24729F] - Flow cytometric analysis of TLR9 in human B cells using 1ug/10^6 cells of NBP2-24908. Cells were then fixed and permeabilized. Then stained with 1 ug of ...read more
Flow Cytometry: TLR9 Antibody (26C593.2) [FITC] [NBP2-24729F] - Analysis using the FITC conjugated version of NBP2-24729. Staining of TLR9 in Ramos cells using 0.5 ug of this antibody. Shaded histogram represents Ramos ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Eq, Pm, PmSpecies Glossary
Applications WB, Simple Western, DB, ELISA, Flow, Func, ICC/IF, IHC, IP, B/N, CyTOF-ready, KD
Clone
26C593.2
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

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TLR9 Antibody (26C593.2) [FITC] Summary

Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-300 of human TLR9 isoform A (Genbank accession no. AAF78037).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TLR9
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Block/Neutralize
  • CyTOF-ready
  • Dot Blot
  • ELISA
  • Flow (Intracellular)
  • Flow Cytometry
  • Functional
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • In vitro assay
  • Knockdown Validated
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Publications
Read Publication using
NBP2-24729F in the following applications:

Reactivity Notes

Rhesus Monkey.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for TLR9 Antibody (26C593.2) [FITC]

  • CD289 antigen
  • CD289
  • TLR9
  • toll-like receptor 9

Background

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TLR9 Antibody (NBP2-24729F)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: FLOW.


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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLR9 Antibody (NBP2-24729F) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional TLR9 Products

Research Areas for TLR9 Antibody (NBP2-24729F)

Find related products by research area.

Blogs on TLR9.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ...  Read full blog post.

Topics in CD11b: The innate immune response
Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens.  Integrin...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR9: For Whom the Cell Tolls
The Toll-like receptor 9 (TLR9) protein, also known as CD289, belongs to the family of Toll-like receptor (TLR) proteins which play a large role in pathogen recognition and the activation of innate immunity. Scientists using TLR9 antibodies have found...  Read full blog post.

TLR9: Tollgate to Immunity
Toll-like receptors (TLRs) play an essential role in the activation of innate immunity, and TLRs are expressed in a large number of immune cells as well as in epithelial cells. TLR9 recognizes synthetic oligodeoxynucleotides (ODN) containing unmethyla...  Read full blog post.

TLR9, Infectious Disease and Cancer
Toll-like receptor 9 (TLR9) is a protein encoded by TLR9 gene in humans. It is also known as cluster of differentiation 289 (CD289) and is a member of TLR family. Proteins from TLR family are transmembrane proteins that expressed in both antigen-resen...  Read full blog post.

TLR9 Antibodies in Immunity Research
Toll-like receptor 9 (TLR9) is a member of the toll-like receptor family that plays a key role in pathogen recognition and activation of innate immunity. Scientists using TLR9 antibodies have found the protein is highly conserved from Drosophila to hu...  Read full blog post.

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Bioinformatics

Gene Symbol TLR9