TLR9 Antibodies
We offer TLR9 Antibodies for use in common research applications: ELISA, Flow (Intracellular), Flow Cytometry, Functional, ICC/IF (Negative), Immunoassay, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, In vitro assay, Intracellular Staining by Flow Cytometry, Sandwich ELISA, Simple Western, Western Blot. Each TLR9 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our TLR9 Antibodies can be used in a variety of model species: Canine, Equine, Human, Mouse, Primate, Rat. Use the list below to choose the TLR9 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our TLR9 polyclonal antibodies and browse our TLR9 monoclonal antibody catalog.
Alternate Names for TLR9 Antibodies
anti-TLR9 antibody, anti-CD289 antibody, anti-CD289 antigen antibody, anti-toll-like receptor 9 antibody
14 Results for "tlr9" in Primary Antibodies
![Simple Western: TLR9 Antibody (26C593.2) [NBP2-24729] - Lane view shows a specific band for TLR9 in 0.5 mg/ml of Ramos lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/TLR9-Antibody-26C593-2-Simple-Western-NBP2-24729-img0029.jpg "Simple Western: TLR9 Antibody (26C593.2) [NBP2-24729] - Lane view shows a specific band for TLR9 in 0.5 mg/ml of Ramos lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system. Image using the Azide Free format of this antibody.")
![Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR9 protein on epithelial cells. HNEC, Detroit-562 and FaDu were stained intracellularly with PE-Ab against TLR9 (open histograms) or appropriate isotype control (shaded histograms) and analyzed by flow cytometry. Representative pictures from one out of three independent experiments are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0098239), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR9-Antibody-26C593-2-Flow-Cytometry-NBP2-24729-img0025.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR-9 protein in HB cells before and after 48 hr of transfection. Beta-actin was used as an internal control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092748) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR9-Antibody-26C593-2-Knockdown-Validated-NBP2-24729-img0031.jpg)
![Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Analysis of colonic LP or peritoneal cavity (PerC) of normal mice were evaluated by flow cytometry. B cells were intracellularly stained with the anti-TLR9 antibody after the cell surface staining with anti-B220 and CD5 antibodies, and examined using flow cytometry. N = 3, performed twice. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0146191) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR9-Antibody-26C593-2-Flow-Cytometry-NBP2-24729-img0027.jpg)
![Immunocytochemistry/Immunofluorescence: TLR9 Antibody (26C593.2) [NBP2-24729] - Cbl-b is required for the endocytic transit of TLR9. Representative confocal microscopic images of splenocytes from mice with indicated genotypes. For experiments, cells were stimulated through the BCR (green) for 30 minutes then fixed and stained for TLR9 (blue) and Lamp-1 (red)(n = 3). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0089792) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR9-Antibody-26C593-2-Immunocytochemistry-Immunofluorescence-NBP2-24729-img0032.jpg)
![Immunohistochemistry-Paraffin: TLR9 Antibody (26C593.2) [NBP2-24729] - Monkey retina tissue. Image from verified customer review.](http://images.novusbio.com/fullsize/TLR9-Antibody-26C593-2-Immunohistochemistry-Paraffin-NBP2-24729-img0023.jpg)
![Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Gating strategy for the detection of TLR-7 and -9 in B cell subsets. PBMC were isolated from whole blood and stained for surface markers before cells were fixed, permeabilised and stained for TLR-9. FSC and SSC were first used to gate out debris and SSC-A and SSC-H was utilized to eliminate duplicates. Further gating was done on CD45 and CD19, to target B cells. To separate between the different B cell populations, we gated on CD27 and IgD (D), followed by TLR-9 expression on these subsets. Data from one representative patient is shown. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0120383) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR9-Antibody-26C593-2-Flow-Cytometry-NBP2-24729-img0028.jpg)
![Western Blot: TLR9 Antibody (26C593.2) [NBP2-24729] - Analysis of TLR9 in A) human PBMC, B) human intestine, C) mouse intestine, and D) rat intestine tissue lysates using this antibody at a dilution of 3 ug/ml.](http://images.novusbio.com/fullsize/TLR9-Antibody-26C593-2-Western-Blot-NBP2-24729-img0033.jpg)
![Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR9 on turbinate epithelial cells from healthy controls compared to turbinate and polyp epithelial cells from patients with CRSwNP, n = 5 (A). Intracellular staining for TLR9 (open histogram, black line) and isotype control (filled histogram) on turbinate epithelial cells from a healthy control (B), turbinate epithelial cells (C) and polyp epithelial cells from a patient (D), analysed using flow cytometry. Results are presented as mean +/- SEM, **P<0.01. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0105618) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR9-Antibody-26C593-2-Flow-Cytometry-NBP2-24729-img0030.jpg)
![Western Blot: TLR9 Antibody [NBP1-76680] - (G) Levels of TLR9 (NBP1-76680), COX4 (NB110-39115), MyD88, and NF-kB protein by Western blot. (H) Relative expression of TLR9 and COX4 protein. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR9-Antibody-Western-Blot-NBP1-76680-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: TLR9 Antibody [NBP1-76680] - (G) Levels of TLR9 (NBP1-76680), COX4 (NB110-39115), MyD88, and NF-kB protein by Western blot. (H) Relative expression of TLR9 and COX4 protein. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TLR9 Antibody [NBP1-76680] - Jurkat cells with TLR9antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/TLR9-Antibody-Immunocytochemistry-NBP1-76680-img0004.jpg)
![Western Blot: TLR9 Antibody [NBP1-76680] - Analysis of TLR9 in Jurkat cell lysate with TLR9 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide](http://images.novusbio.com/fullsize/TLR9-Antibody-Western-Blot-NBP1-76680-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: TLR9 Antibody [NBP1-76680] - (A) Levels of COX4 (NB110-39115), TLR9 (NBP1-76680), MyD88, and NF-kB mRNA. (B) Levels of COX4 (NB110-39115), TLR9 (NBP1-76680), MyD88, and NF-kB protein by Western blot. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR9-Antibody-Western-Blot-NBP1-76680-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: TLR9 Antibody [NBP1-76680] - Mouse Spleen tissue.](http://images.novusbio.com/fullsize/TLR9-Antibody-Immunofluorescence-NBP1-76680-img0003.jpg)
![Western Blot: TLR9 Antibody - BSA Free [NBP1-76680] - Levels of toll-like receptor (TLR) 9, myeloid differentiation factor 88 (MyD88), & nuclear factor (NF)-kappa B in lung tissues from animals with spontaneous breathing (CON), mechanical ventilation at normal tidal volume (NTV), high tidal volume (HTV), & ODN2088 pretreatment upon HTV stimulation (HTV + ODN). aP < 0.05 vs. CON group;bP < 0.05 vs. NTV group; & cP < 0.05 vs. HTV group. Image collected & cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76680_rabbit-polyclonal-tlr9-antibody-31020241535698.jpg)
![Western Blot: TLR9 Antibody - BSA Free [NBP1-76680] - The mRNA & protein expression of cytochrome c oxidase 4 (COX4), toll-like receptor (TLR) 9, myeloid differentiation factor 88 (MyD88), & nuclear factor (NF)-kappa B in lung tissues from spontaneous breathing group (CON group), normal tidal volume (NTV) group, high tidal volume (HTV) group, chloroquine (CQ) pretreatment upon HTV stimulation group (HTV + CQ). (A) Levels of COX4, TLR9, MyD88, & NF-kappa B mRNA. (B) Levels of COX4, TLR9, MyD88, & NF-kappa B protein by Western blot. (C) Relative expression of COX4, TLR9, MyD88, & NF-kappa B protein. Fold expression for target genes was normalized to that measured for the beta -actin gene. Both of these experiments were in triplicate. aP < 0.05 vs. CON group; bP < 0.05 vs. NTV group; & CP < 0.05 vs. HTV group. Image collected & cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76680_rabbit-polyclonal-tlr9-antibody-31020241616025.jpg)
![Flow Cytometry: TLR9 Antibody (eB72-1665) [NBP1-43140] - Analysis using the PE conjugate of NBP1-43140. Staining of non-transfected (left) and human CD289-transfected (right) HEK 293T cells with 1.0 ug of Rat IgG2a kappa Isotype Control PE (open histogram) or 1.0 ug of Anti-Human CD289 (TLR9) PE (filled histogram). Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/TLR9-Antibody-eB72-1665-Flow-Cytometry-NBP1-43140-img0006.jpg "Flow Cytometry: TLR9 Antibody (eB72-1665) [NBP1-43140] - Analysis using the PE conjugate of NBP1-43140. Staining of non-transfected (left) and human CD289-transfected (right) HEK 293T cells with 1.0 ug of Rat IgG2a kappa Isotype Control PE (open histogram) or 1.0 ug of Anti-Human CD289 (TLR9) PE (filled histogram). Total viable cells were used for analysis.")
![Flow Cytometry: TLR9 Antibody (eB72-1665) [NBP1-43140] - HEK 293 parent cells (left panel) and 293/hTLR9 transfected cells (right panel) were fixed and permeabilized and subsequently stained with either Anti-Rat IgG FITC alone (thin line) or with 1 micrograms of Anti-Human CD289 (TLR9) Purified followed by Anti-Rat IgG FITC (thick line).](http://images.novusbio.com/fullsize/TLR9-Antibody-eB72-1665-Flow-Cytometry-NBP1-43140-img0003.jpg)
![Flow Cytometry: TLR9 Antibody (eB72-1665) [NBP1-43140] - HEK 293 parent cells (left panel) and 293/hTLR9 transfected cells (right panel) were fixed and permeabilized and subsequently stained with either Anti-Rat IgG FITC alone (thin line) or with 1 micrograms of Anti-Human CD289 (TLR9) Purified followed by Anti-Rat IgG FITC (thick line).](http://images.novusbio.com/fullsize/TLR9-Antibody-eB72-1665-Flow-Cytometry-NBP1-43140-img0004.jpg)
![Western Blot: TLR9 Antibody (2A4C6.2E5) [NBP2-31150] - Mouse Liver (lane 1), Mouse Kidney (lane 2) and Mouse Spleen (lane 3) were separated by SDS-PAGE and the protein transferred to PVDF. The membrane was then probed with anti-TLR9 at 2ug/mL and detected with an anti-rat HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/TLR9-Antibody-2E5-Western-Blot-NBP2-31150-img0007.jpg "Western Blot: TLR9 Antibody (2A4C6.2E5) [NBP2-31150] - Mouse Liver (lane 1), Mouse Kidney (lane 2) and Mouse Spleen (lane 3) were separated by SDS-PAGE and the protein transferred to PVDF. The membrane was then probed with anti-TLR9 at 2ug/mL and detected with an anti-rat HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: TLR9 Antibody (2A4C6.2E5) [NBP2-31150] - Analysis of TLR9 protein in normal mouse epididymis section using TLR9 antibody (clone 2E5) at a concentration of 5 ug/mL. This antibody developed strong cytoplasmic staining in the pseudostratified columnar epithelial cells as well as the blood vessels of epididymis. The basal cells, the smooth muscle cells and the mature sperm cells were largely found negative for TLR9 staining.](http://images.novusbio.com/fullsize/TLR9-Antibody-2E5-Immunohistochemistry-Paraffin-NBP2-31150-img0006.jpg)
![Western Blot: TLR9 Antibody (2A4C6.2E5) [NBP2-31150] - TLR9 Antibody (2E5) [NBP2-31150] - TLR9 Antibody (2E5) [NBP2-31150] - WB analysis of partial recombinant TLR9 protein (expected molecular weight ~21kDa) with TLR9 antibody (clone 2A4C6.2E5) at a concentration of 2 ug/mL.](http://images.novusbio.com/fullsize/TLR9-Antibody-2E5-Western-Blot-NBP2-31150-img0004.jpg)
![Western Blot: TLR9 Antibody [NBP1-77254] - Mouse spleen cell lysate with TLR9 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.](http://images.novusbio.com/fullsize/TLR9-Antibody-Western-Blot-NBP1-77254-img0005.jpg "Western Blot: TLR9 Antibody [NBP1-77254] - Mouse spleen cell lysate with TLR9 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: TLR9 Antibody [NBP1-77254] - Mouse spleen cells 10 ug/ml.](http://images.novusbio.com/fullsize/TLR9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77254-img0003.jpg)
![Immunohistochemistry-Paraffin: TLR9 Antibody [NBP1-77254] - Mouse spleen cells with TLR9 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/TLR9-Antibody-Immunohistochemistry-Paraffin-NBP1-77254-img0004.jpg)
![Flow Cytometry: TLR9 Antibody (5G5) [NBP1-51156] - THP1 cells were incubated with IgG2a isptype controle Cells (140000) were permeabilized with saponin and stained with 0.4 ug 5G5](http://images.novusbio.com/fullsize/TLR9-Antibody-5G5-Flow-Cytometry-NBP1-51156-img0006.jpg "Flow Cytometry: TLR9 Antibody (5G5) [NBP1-51156] - THP1 cells were incubated with IgG2a isptype controle Cells (140000) were permeabilized with saponin and stained with 0.4 ug 5G5")
![Flow Cytometry: TLR9 Antibody (5G5) [NBP1-51156] - THP1 cells were incubated with IgG2a isptype controle (A) or a-TLR9 5G5 mAb](http://images.novusbio.com/fullsize/TLR9-Antibody-5G5-Flow-Cytometry-NBP1-51156-img0005.jpg)
