Western blot is one of the most commonly used techniques for biomarker profiling in basic and translational research. For making it convenient and to accelerate the analysis, we offer INSTA-Blot product which is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. INSTA-Blot offers a streamlined western blotting procedure which limits the procedure to six steps - blocking, primary antibody incubation, washing, secondary antibody incubation, washing, and WB development. Novus Biologicals offers INSTA-Blot for Human, Rat (Sprague Dawley strain) and Mouse (Balb/c strain) species with membranes having multiple lanes containing molecular weight marker and lysates of various tissues - Brain, Heart, Small Intestine, Kidney, Liver, Lung, Skeletal Muscle, Stomach, Spleen, Ovary and Testis. We also have INSTA-Blots for individual tissues as well as multiple tissues-multi-species membranes (human, mouse and rat). The human OncoPair INSTA-Blots contains the membranes with lysates from cancerous and normal tissues of the same patient. Human cell line INSTA-Blot comes with lysates of - HeLa, Jurkat, Daudi, HEK 293, Rh30, A375, T98G, HCT-116, Hep-2 cells. Complete product details including the amido black stained blot images, citations in publications, product reviews and related products may be seen on the individual online datasheets of the listed products.
![Western Blot: INSTA-Blot Human Tissues [NBP2-30113] - Human Tissues. Approximately 20 ug per lane of human tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker (Mark12); 2. Brain; 3. Heart; 4. Small Intestine; 5. Kidney; 6. Liver; 7. Lung; 8. Skeletal Muscle; 9. Stomach; 10. Spleen; 11. Ovary; 12. Testis](http://images.novusbio.com/fullsize/INSTA-Blot-Human-Tissues-SDS-Page-NBP2-30113-img0001.jpg "Western Blot: INSTA-Blot Human Tissues [NBP2-30113] - Human Tissues. Approximately 20 ug per lane of human tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker (Mark12); 2. Brain; 3. Heart; 4. Small Intestine; 5. Kidney; 6. Liver; 7. Lung; 8. Skeletal Muscle; 9. Stomach; 10. Spleen; 11. Ovary; 12. Testis")
![Western Blot: INSTA-Blot Breast Tissue OncoPair [NBP2-30125] - Western Blot: INSTA-Blot Breast Tissue OncoPair [NBP2-30125] - Representative amido-black stained blot. Samples on the blot are laid out in pairs of tumor and normal tissue lysate from the same patient. There are seven pairs of samples, representing seven different patients, on each OncoPair blot. Note: The blot image is representative only.](http://images.novusbio.com/fullsize/INSTA-Blot-Breast-Tissue-OncoPair-Western-Blot-NBP2-30125-img0003.jpg "Western Blot: INSTA-Blot Breast Tissue OncoPair [NBP2-30125] - Western Blot: INSTA-Blot Breast Tissue OncoPair [NBP2-30125] - Representative amido-black stained blot. Samples on the blot are laid out in pairs of tumor and normal tissue lysate from the same patient. There are seven pairs of samples, representing seven different patients, on each OncoPair blot. Note: The blot image is representative only.")
![INSTA-Blot Breast Tissue OncoPair [NBP2-30125] - Tumor tissue is taken from healthy tumor tissue while normal tissue is taken from an area adjacent to the tumor tissue.](http://images.novusbio.com/fullsize/INSTA-Blot-Breast-Tissue-OncoPair-NBP2-30125-img0001.jpg)
![Western Blot: INSTA-Blot Male Lung Tissue OncoPair [NBP2-30122] - Representative amido-black stained blot. Samples on the blot are laid out in pairs of tumor and normal tissue lysate from the same patient. There are seven pairs of samples, representing seven different patients, on each OncoPair blot. Note: The blot image is representative only. The actual image on your blot may vary.](http://images.novusbio.com/fullsize/INSTA-Blot-Male-Lung-Tissue-OncoPair-Western-Blot-NBP2-30122-img0003.jpg "Western Blot: INSTA-Blot Male Lung Tissue OncoPair [NBP2-30122] - Representative amido-black stained blot. Samples on the blot are laid out in pairs of tumor and normal tissue lysate from the same patient. There are seven pairs of samples, representing seven different patients, on each OncoPair blot. Note: The blot image is representative only. The actual image on your blot may vary.")
![INSTA-Blot Male Lung Tissue OncoPair [NBP2-30122] - Tumor tissue is taken from healthy tumor tissue while normal tissue is taken from an area adjacent to the tumor tissue.](http://images.novusbio.com/fullsize/INSTA-Blot-Male-Lung-Tissue-OncoPair-NBP2-30122-img0001.jpg)
![Western Blot: INSTA-Blot Female Lung Tissue OncoPair [NBP2-30123] - Representative amido-black stained blot. Samples on the blot are laid out in pairs of tumor and normal tissue lysate from the same patient. There are seven pairs of samples, representing seven different patients, on each OncoPair blot. Note: The blot image is representative only.](http://images.novusbio.com/fullsize/INSTA-Blot-Female-Lung-Tissue-OncoPair-Western-Blot-NBP2-30123-img0002.jpg "Western Blot: INSTA-Blot Female Lung Tissue OncoPair [NBP2-30123] - Representative amido-black stained blot. Samples on the blot are laid out in pairs of tumor and normal tissue lysate from the same patient. There are seven pairs of samples, representing seven different patients, on each OncoPair blot. Note: The blot image is representative only.")
![INSTA-Blot Female Lung Tissue OncoPair [NBP2-30123] - Tumor tissue is taken from healthy tumor tissue while normal tissue is taken from an area adjacent to the tumor tissue.](http://images.novusbio.com/fullsize/INSTA-Blot-Female-Lung-Tissue-OncoPair-NBP2-30123-img0001.jpg)
![Western Blot: INSTA-Blot Human Tissues [NBP2-31378] - SDS-Page: INSTA-Blot Human Tissues [NBP2-31378] - Approximately 50 ug per lane of human tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker; 2. Brain; 3. Heart; 4. Small Intestine; 5. Kidney; 6. Liver; 7. Lung; 8. Skeletal Muscle; 9. Stomach; 10. Spleen; 11. Ovary; 12. Testis](http://images.novusbio.com/fullsize/INSTA-Blot-Human-Tissues-Western-Blot-NBP2-31378-img0001.jpg "Western Blot: INSTA-Blot Human Tissues [NBP2-31378] - SDS-Page: INSTA-Blot Human Tissues [NBP2-31378] - Approximately 50 ug per lane of human tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker; 2. Brain; 3. Heart; 4. Small Intestine; 5. Kidney; 6. Liver; 7. Lung; 8. Skeletal Muscle; 9. Stomach; 10. Spleen; 11. Ovary; 12. Testis")
![SDS-Page: INSTA-Blot Colon Tissue OncoPair [NBP2-30129] - Samples on the blot are layed out in pairs of tumor and normal tissue lysate from the same patient. There are seven pairs of samples, representing seven different patients, on each OncoPair blot.](http://images.novusbio.com/fullsize/INSTA-Blot-Colon-Tissue-OncoPair-SDS-Page-NBP2-30129-img0001.jpg "SDS-Page: INSTA-Blot Colon Tissue OncoPair [NBP2-30129] - Samples on the blot are layed out in pairs of tumor and normal tissue lysate from the same patient. There are seven pairs of samples, representing seven different patients, on each OncoPair blot.")
![INSTA-Blot Colon Tissue OncoPair [NBP2-30129] - Tumor tissue is taken from healthy tumor tissue while normal tissue is taken from an area adjacent to the tumor tissue.](http://images.novusbio.com/fullsize/INSTA-Blot-Colon-Tissue-OncoPair-NBP2-30129-img0002.jpg)
![Western Blot: INSTA-Blot Human Cell Lines [NBP2-30114] - Human Cell Lines. Approximately 20 ug per lane of human cell line lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker (Mark12); 2. HeLa; 3. Jurkat; 4. Daudi; 5. HEK 293; 6. Rh30; 7. A375; 8. T98G; 9. HCT-116; 10. Hep-2.](http://images.novusbio.com/fullsize/INSTA-Blot-Human-Cell-Lines-SDS-Page-NBP2-30114-img0001.jpg "Western Blot: INSTA-Blot Human Cell Lines [NBP2-30114] - Human Cell Lines. Approximately 20 ug per lane of human cell line lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker (Mark12); 2. HeLa; 3. Jurkat; 4. Daudi; 5. HEK 293; 6. Rh30; 7. A375; 8. T98G; 9. HCT-116; 10. Hep-2.")
![Western Blot: INSTA-Blot Ovary Tissue OncoPair [NBP2-30124] - Representative amido-black stained blot. Samples on the blot are laid out in pairs of tumor and normal tissue lysate from the same patient. There are seven pairs of samples, representing seven different patients, on each OncoPair blot. Note: The blot image is representative only.](http://images.novusbio.com/fullsize/INSTA-Blot-Ovary-Tissue-OncoPair-Western-Blot-NBP2-30124-img0002.jpg "Western Blot: INSTA-Blot Ovary Tissue OncoPair [NBP2-30124] - Representative amido-black stained blot. Samples on the blot are laid out in pairs of tumor and normal tissue lysate from the same patient. There are seven pairs of samples, representing seven different patients, on each OncoPair blot. Note: The blot image is representative only.")
![INSTA-Blot Ovary Tissue OncoPair [NBP2-30124] - Tumor tissue is taken from healthy tumor tissue while normal tissue is taken from an area adjacent to the tumor tissue.](http://images.novusbio.com/fullsize/INSTA-Blot-Ovary-Tissue-OncoPair-NBP2-30124-img0001.jpg)
![SDS-Page: INSTA-Blot Mouse Tissues [NBP2-30111] - Mouse Tissues. Approximately 20 ug per lane of different mouse tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker (Mark12); 2. Brain; 3. Heart; 4. Small Intestine; 5. Kidney; 6. Liver; 7. Lung; 8. Muscle; 9. Stomach; 10. Spleen; 11. Ovary; 12. Testis](http://images.novusbio.com/fullsize/INSTA-Blot-Mouse-Tissues-SDS-Page-NBP2-30111-img0001.jpg "SDS-Page: INSTA-Blot Mouse Tissues [NBP2-30111] - Mouse Tissues. Approximately 20 ug per lane of different mouse tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker (Mark12); 2. Brain; 3. Heart; 4. Small Intestine; 5. Kidney; 6. Liver; 7. Lung; 8. Muscle; 9. Stomach; 10. Spleen; 11. Ovary; 12. Testis")
![SDS-Page: INSTA-Blot Rat Tissues [NBP2-30112] - Rat Tissues. Approximately 20 ug per lane of different rat tissue lysate are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker; 2. Brain; 3. Heart; 4. Small Intestine; 5. Kidney; 6. Liver; 7. Lung; 8. Skeletal Muscle; 9. Stomach; 10. Spleen; 11. Ovary; 12. Testis](http://images.novusbio.com/fullsize/INSTA-Blot-Rat-Tissues-SDS-Page-NBP2-30112-img0006.jpg "SDS-Page: INSTA-Blot Rat Tissues [NBP2-30112] - Rat Tissues. Approximately 20 ug per lane of different rat tissue lysate are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker; 2. Brain; 3. Heart; 4. Small Intestine; 5. Kidney; 6. Liver; 7. Lung; 8. Skeletal Muscle; 9. Stomach; 10. Spleen; 11. Ovary; 12. Testis")
![SDS-Page: INSTA-Blot Human Brain [NBP2-30119] - Approximately 30 ug per lane of tissue lysate is resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker; 2-12 human brain.](http://images.novusbio.com/fullsize/INSTA-Blot-Human-Brain-SDS-Page-NBP2-30119-img0001.jpg "SDS-Page: INSTA-Blot Human Brain [NBP2-30119] - Approximately 30 ug per lane of tissue lysate is resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker; 2-12 human brain.")