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Recombinant Human TNF-alpha Protein

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Recombinant Human TNF-alpha (Catalog # 210-TA) has a molecular weight (MW) of 53.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Recombinant Human TNF-alpha (Catalog # 210-TA) has a molecular weight (MW) of 53.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational ...read more
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
210-TA-02M/CF
Carrier Free
210-TA-020/CF
Carrier Free
210-TA-100/CF
Carrier Free
210-TA-005/CF
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Recombinant Human TNF-alpha Protein Summary

Additional Information
Analyzed by SEC-MALS
Details of Functionality
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL.
Source
E. coli-derived human TNF-alpha protein
Val77-Leu233, with and without an N-terminal Met
Accession #
N-terminal Sequence
Met & Val77
Protein/Peptide Type
Recombinant Proteins
Gene
TNF
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17 kDa, reducing conditions
Reviewed Applications
Read 5 Reviews rated 4.6
using
210-TA in the following application:
Publications
Read Publications using
210-TA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 0.1-1 mg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TNF-alpha Protein

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (1, 2). Human TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) (3). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha . TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells (1, 2). TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (4). Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I (2, 5). Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (6-8). TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (9, 10) and the hematopoietic cell-restricted 80 kDa TNF RII (11, 12), both of which are also expressed as homotrimers (1, 2, 13). Both type I and type II receptors bind TNF-alpha with comparable affinity (14), although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha (15).
  1. Zelova, H. and J. Hosek (2013) Inflamm. Res. 62:641.
  2. Juhasz, K. et al. (2013) Expert Rev. Clin. Immunol. 9:335.
  3. Pennica, D. et al. (1984) Nature 312:724.
  4. Tang, P. et al. (1996) Biochemistry 35:8216.
  5. Perez, C. et al. (1990) Cell 63:251.
  6. Black, R.A. et al. (1997) Nature 385:729.
  7. Moss, M.L. et al. (1997) Nature 385:733.
  8. Gearing, A.J.H. et al. (1994) Nature 370:555.
  9. Schall, T.J. et al. (1990) Cell 61:361.
  10. Loetscher, H. et al. (1990) Cell 61:351.
  11. Dembic, Z. et al. (1990) Cytokine 2:231.
  12. Smith, C.A. et al. (1990) Science 248:1019.
  13. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
  14. Pinckard, J.K. et al. (1997) J. Biol. Chem. 272:10784.
  15. Engelmann, H. et al. (1990) J. Biol. Chem. 265:1531.

Publications for TNF-alpha (210-TA)(799)

We have publications tested in 19 confirmed species: Human, Mouse, Rat, Bovine, Canine, Complex Species Category, Drosophila, Feline, Guinea Pig, Hamster, N/A, Ovine, Porcine, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Rabbit, Transgenic Mouse,  Human.

We have publications tested in 14 applications: Bioassay, Cell Culture, ELISA (Capture), ELISA (Standard), ELISA Control, ELISA Standard, Hemofiltration Procedure, IHC-P, In Vivo, SDS-Page, Spheroid Culture, Stimulation, Surface Plasmon Resonance (SPR, Western Blot.


Filter By Application
Bioassay
(716)
Cell Culture
(45)
ELISA (Capture)
(1)
ELISA (Standard)
(15)
ELISA Control
(1)
ELISA Standard
(2)
Hemofiltration Procedure
(1)
IHC-P
(1)
In Vivo
(6)
SDS-Page
(1)
Spheroid Culture
(1)
Stimulation
(3)
Surface Plasmon Resonance (SPR
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(716)
Mouse
(39)
Rat
(10)
Bovine
(6)
Canine
(2)
Complex Species Category
(2)
Drosophila
(1)
Feline
(1)
Guinea Pig
(1)
Hamster
(1)
N/A
(2)
Ovine
(1)
Porcine
(4)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(2)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(5)
Rabbit
(1)
Transgenic Mouse
(1)
 Human
(1)
All Species
Showing Publications 1 - 10 of 799. Show All 799 Publications.
Publications using 210-TA Applications Species
Jin, Y;Zhang, D;Deng, K;Wu, P;Yang, D;Xie, Z;Qiu, W;Yu, G; Role of the cAMP-PKA-NF-?B pathway in Mucin1 over-expression in A549 cells during Respiratory syncytial virus infection BMC infectious diseases 2023-11-30 [PMID: 38036963] (Bioassay, Human) Bioassay Human
Abu El-Asrar, AM;Nawaz, MI;Ahmad, A;Dillemans, L;Siddiquei, M;Allegaert, E;Gikandi, PW;De Hertogh, G;Opdenakker, G;Struyf, S; CD40 Ligand-CD40 Interaction Is an Intermediary between Inflammation and Angiogenesis in Proliferative Diabetic Retinopathy International journal of molecular sciences 2023-10-25 [PMID: 37958563] (Bioassay, Human) Bioassay Human
Çapk?n, E;Kutlu, A;Yüce, M; Repurposing Fc gamma receptor I (Fc?RI, CD64) for site-oriented monoclonal antibody capture: A proof-of-concept study for real-time detection of tumor necrosis factor-alpha (TNF -?) Heliyon 2023-09-01 [PMID: 37809995] (Bioassay, N/A) Bioassay N/A
Britt, EC;Qing, X;Votava, JA;Lika, J;Wagner, A;Shen, S;Arp, NL;Khan, H;Schieke, SM;Fletcher, CD;Huttenlocher, A;Fan, J; Activation-induced shift in nutrient preference and function-specific nutrient dependence in human neutrophils bioRxiv : the preprint server for biology 2023-09-26 [PMID: 37808750] (Cell Culture, Human) Cell Culture  Human
Munir, M;Embry, A;Doench, JG;Heaton, NS;Wilen, CB;Orchard, RC; Genome-wide CRISPR activation screen identifies JADE3 as an antiviral activator of NF-kB bioRxiv : the preprint server for biology 2023-09-29 [PMID: 37808733] (Bioassay, Human) Bioassay Human
Pike, CM;Zwarycz, B;McQueen, BE;Castillo, M;Barron, C;Morowitz, JM;Levi, JA;Phadke, D;Balik-Meisner, M;Mav, D;Shah, R;Glasspoole, DLC;Laetham, R;Thelin, W;Bunger, MK;Boazak, EM; Characterization and optimization of variability in a human colonic epithelium culture model bioRxiv : the preprint server for biology 2023-09-22 [PMID: 37790345] (Bioassay, Human) Bioassay Human
Wright, CW;Li, N;Shaffer, L;Hill, A;Boyer, N;Alves, SE;Venkataraman, S;Biswas, K;Lieberman, LA;Mohammadi, S; Establishment of a 96-well transwell system using primary human gut organoids to capture multiple quantitative pathway readouts Scientific reports 2023-09-29 [PMID: 37773535] (Bioassay, Human) Bioassay Human
Yang, YS;Lin, C;Ma, H;Xie, J;Kaplan, FS;Gao, G;Shim, JH; AAV-Mediated Targeting of the Activin A-ACVR1R206H Signaling in Fibrodysplasia Ossificans Progressiva Biomolecules 2023-09-08 [PMID: 37759764] (Bioassay, Human, Transgenic Mouse) Bioassay Human, Transgenic Mouse
Korde, A;Haslip, M;Pednekar, P;Khan, A;Chioccioli, M;Mehta, S;Lopez-Giraldez, F;Bermejo, S;Rojas, M;Dela Cruz, C;Matthay, MA;Pober, JS;Pierce, RW;Takyar, SS; MicroRNA-1 protects the endothelium in acute lung injury JCI insight 2023-09-22 [PMID: 37737266] (Bioassay, Human) Bioassay Human
Yoshida, K;Suzuki, S;Yuan, H;Sato, A;Hirata-Tsuchiya, S;Saito, M;Yamada, S;Shiba, H; Public RNA-seq data-based identification and functional analyses reveal that MXRA5 retains proliferative and migratory abilities of dental pulp stem cells Scientific reports 2023-09-20 [PMID: 37730838] (Bioassay, Human) Bioassay Human
Show All 799 Publications.

Reviews for TNF-alpha (210-TA) (5) 4.65

Average Rating: 4.6
(Based on 5 reviews)

Reviews using 210-TA:
Filter by Applications
Apoptosis assay
(1)
CellProlif
(4)
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Russ Tas
 Apoptosis assay 08/14/2023
View

Summary

ApplicationApoptosis assay
LotDDHB0422043
  4
reviewed by:
Russ Tas
 CellProlif 08/07/2023
View

Summary

ApplicationCell Proliferation
LotDDHB0422043
  5
reviewed by:
Verified Customer
 CellProlif 02/04/2019
View

Summary

ApplicationCell Proliferation
Lot87323
  5
reviewed by:
Verified Customer
 CellProlif 01/29/2019
View

Summary

ApplicationCell Proliferation
Lot87324
  5
reviewed by:
Verified Customer
 CellProlif 05/09/2018
View

Summary

ApplicationCell Proliferation
Lot87325

FAQs for TNF-alpha (210-TA). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Additional TNF-alpha Products

Blogs on TNF-alpha. Showing 1-10 of 13 blog posts - Show all blog posts.

Check out the latest blog posts on TNF-alpha.
Unlocking the Potential of Biosimilars in Immuno-Oncology
By Jennifer Jones, M.S.Biosimilar Antibodies: Imitation Meets InnovationIn the ever-evolving medical landscape, a new class of pharmaceuticals is emerging as a game-changer, poised to transform the way we approach...  Read full blog post.
Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.
Immune Cell Metabolic Flux Influences Type I Diabetes
By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev...  Read full blog post.

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Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.
Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.
Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.
You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.
Showing 1-10 of 13 blog posts - Show all blog posts.

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Recent Reviews

4.6
5
3
4
2
3
0
2
0
1
0
 
 Russ Tas
08/14/2023
Application: Apoptosis assay
Species:
read this review
 
 Russ Tas
08/07/2023
Application: CellProlif
Species:
read this review
 
 Verified Customer
02/04/2019
Application: CellProlif
Species:
read this review

Bioinformatics

Gene Symbol TNF
Uniprot