I have a question regarding the product NBP2-29328 and want to know how the inhibitory peptide passes through the cell wall?

The MyD88 inhibitory peptide contains a protein transduction (PTD) sequence derived from antennapedia which renders the PTD sequence renders the peptide cell permeable. The antennapedia technology was first described by Derossi et al, The third helix of the Antennapedia homeodomain translocates through biological membranes and is used in a number of our inhibitory peptides. The MyD88 inhibitory peptide also contains a sequence from the MyD88 TIR homodimerization domain (Loiarro et al, Peptide-mediated interference of TIR domain dimerization in MyD88 inhibits interleukin-1-dependent activation of NF-{kappa}B). The control peptide consists of only the PTD sequence. The Derossi and Loiarro technology references are attached for additional background information. Please also follow this link to representative publications citing the MyD88 inhibitory peptide.

I'm looking into purchasing NBP2-29328 for use and I am wondering if you can give me more information. Specifically, I would like to inject this drug ICM (directly into the CNS) using a rat model, and I am wondering what information you can provide about

Unfortunately, I do not see any in-vivo data for this product on our internal records. Most of the information that we have on our records for this product may be seen at its online datasheets

I was wondering whether NBP2-29328-5mg has biological activity.

Our MyD88 Inhibitor Peptide Set does have biological activity.

Is this the same as ST2825 compound described by Loiarro et al, 2007?

ST2825 is the heptapeptide only, whereas the inhibitor peptide from NBP2-29328 corresponds to the association between the hexapeptide + the PTD sequence from antennapedia. In that case, this would suggest that the product used in Loiarro et al. is not the same as NBP2-29328.

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MyD88 Inhibitor Peptide Set

(99 Publications)

(3 Reviews)

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Product Details

Summary

Reactivity	Hu, Mu, Rt, Bv, Xp, Ye, ZeSpecies Glossary
Applications	ELISA, Flow, Func, IHC, Inhibition Activity, B/N
Concentration	LYOPH

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MyD88 Inhibitor Peptide Set Summary

Immunogen	Functions as a decoy by binding to the MyD88 TIR domain.
Specificity	The MyD88 Homodimerization inhibitory peptide contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable (Derossi et al, The third helix of the antennapedia homeodomain translocates through biological membranes. J Biol Chem. 269:10444-10450 (1994)] .The control peptide consists of only the PTD sequence.
Preparation Method	Preparation of 5 mM Stock Solutions PBS* is added directly to the vials to prepare the stock solutions. Note: Bring the solution to room temperature and quick spin the tubes before opening the caps. MyD88 Homodimerization Inhibitor Peptide: 1 mg of DRQIKIWFQNRRMKWKKRDVLPGT Add 64.4 ul of PBS* to the vial to make a 5 mM stock solution. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing. Control Peptide: 1 mg of DRQIKIWFQNRRMKWKK Add 84.8 ul PBS* to the vial. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing *Recipe for 1X PBS: 1. Dissolve the following in 800ml distilled H2O. - 8g of NaCl - 0.2g of KCl - 1.44g of Na2HPO4 - 0.24g of KH2PO4 2. Adjust pH to 7.5 with HCl. 3. Adjust volume to 1L with additional distilled H2O. 4. Sterilize by autoclaving
Content	2 x 1.0mg: MyD88 Homodimerization Inhibitor peptide (lyophilized) DRQIKIWFQNRRMKWKKRDVLPGT (MyD88 homodimerization sequence: RDVLPGT). Molecular weight: 3100 2 x 1.0mg: Antennapedia Control peptide (lyophilized) DRQIKIWFQNRRMKWKK. Molecular weight: 2361 5mg size will contain 5mg of each peptide (MyD88 Homodimerization Inhibitor peptide and Antennapedia Control peptide).
Gene	MYD88

Applications/Dilutions

Dilutions

Binding Inhibition reported in scientific literature (PMID 28302163)
Block/Neutralize reported in scientific literature (PMID 24886588)
ELISA 1:100 - 1:2000. Use reported in scientific literature (PMID 23469306)
Flow Cytometry reported in scientific literature (PMID 27870876)
Functional (Inhibition) reported in multiple pieces of scientific literature
Immunohistochemistry reported in scientific literature (PMID 28282921)
In vitro assay reported in scientific literature (PMID 25810567)
In vivo assay reported in scientific literature (PMID 24755282)

Application Notes

Inhibition of MyD88 dependent TLR/IL-1R signaling activitiy by interefering with MyD88 homodimer formation.

Inhibitory peptide at 100 uM concentration may be a starting point. However, useful concentration of peptide may vary depending on experimental condition and cell type. Incubate cells for 24 hr with peptides before stimulating with ligands.

Reviewed Applications

Read 3 Reviews rated 5

using
NBP2-29328 in the following applications:

Publications

Read Publications using
NBP2-29328 in the following applications:

B/N
13 publications
BindInhib
2 publications
ELISA
2 publications
FLOW
1 publication
Forced Spectroscopy
7 publications
Func
2 publications
Func-Inh
1 publication
IF/IHC
2 publications
In Vivo
6 publications
In vitro
6 publications
In-Cell Western (ICW)
2 publications
WB
4 publications

Reactivity Notes

Yeast reactivity reported in scientific literature (PMID: 27870876).

Packaging, Storage & Formulations

Storage	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer	Solubilize the peptides prior to use by making 5 mM PBS* stock solutions (please see Preparation of 5 mM Stock Solutions under Preparation Method).
Concentration	LYOPH
Reconstitution Instructions	Please contact technical support for detailed reconstitution instructions.

Alternate Names for MyD88 Inhibitor Peptide Set

MyD88
MYD88D
myeloid differentiation primary response gene (88)
myeloid differentiation primary response protein MyD88

Background

MyD88 is an adapter protein that links Toll-like receptors (TLRs) and Interleukin-1 receptors (IL-1rs) with downstream signaling molecules. Homodimerization of MyD88 is a critical step in the signaling process, and allows the recruitment and activation of the kinase IRAK. MyD88 exists as a homodimer when recruited to activated TLR/IL-1Rs. The inhibitor peptide contains a sequence from the MyD88 TIR homodimerization domain (Loiarro, et al.). 1 MyD88 monomer binds to this inhibitor peptide, there by blocking MyD88 homodimerization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.

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Publications for MyD88 Inhibitor (NBP2-29328)(99)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Porcine, Yeast.

We have publications tested in 12 applications: B/N, BindInhib, ELISA, FLOW, Forced Spectroscopy, Func, Func-Inh, IF/IHC, In Vivo, In vitro, In-Cell Western (ICW), WB.

Showing Publications 1 - 10 of 99. Show All 99 Publications.

Publications using NBP2-29328	Applications	Species
Yao XP, Ye J, Feng T et al. Adaptor protein MyD88 confers the susceptibility to stress via amplifying immune danger signals Brain, behavior, and immunity 2022-12-07 [PMID: 36496170] (In Vivo)	In Vivo
Katab E, Kumar A, Steiger K et al. NF-?B c-REL-OTUD4 axis regulates B-cell receptor in B-cell lymphoma bioRxiv 2023-05-08
Suresh B THE ROLE OF LYSOSOME REMODELING IN PHAGOCYTOSIS Thesis 2021-01-01 (Forced Spectroscopy)	Forced Spectroscopy
Noya M Searching for pattern recognition receptor (s) involved in the response to tick-borne encephalitis virus (TBEV) and the TBEV vaccine Thesis 2020-01-01
Zainal Abidin N. Studies on the intracellular life of the melioidosis pathogen Burkholderia pseudomallei. Thesis. 2018-01-01 (B/N, Mouse)	B/N	Mouse
Saxena A. Defining the Impact of Metabolic Stress and the loss of NOD2 in Epithelial-microbial Interaction. Thesis 2017-01-01 (Human)		Human
Sahanic S, Hilbe R, Dünser C et al. SARS-CoV-2 activates the TLR4/MyD88 pathway in human macrophages: A possible correlation with strong pro-inflammatory responses in severe COVID-19 Heliyon 2023-11-01 [PMID: 38034686]
Thapa HB, Kohl P, Zingl FG et al. Characterization of the Inflammatory Response Evoked by Bacterial Membrane Vesicles in Intestinal Cells Reveals an RIPK2-Dependent Activation by Enterotoxigenic Escherichia coli Vesicles Microbiology spectrum 2023-06-12 [PMID: 37306596] (Func-Inh)	Func-Inh
Kwon O, Lee S Ishige okamurae Attenuates Neuroinflammation and Cognitive Deficits in Mice Intracerebroventricularly Injected with LPS via Regulating TLR-4/MyD88-Dependent Pathways Antioxidants 2022-12-29 [PMID: 36670940]
Liao Y, Guo C, Wen A et al. Frankincense-Myrrh treatment alleviates neuropathic pain via the inhibition of neuroglia activation mediated by the TLR4/MyD88 pathway and TRPV1 signaling Phytomedicine : international journal of phytotherapy and phytopharmacology 2023-01-01 [PMID: 36379093] (In Vivo, Mouse)	In Vivo	Mouse
Chen M, Deng H, Zhao Y et al. Toll-Like Receptor 2 Modulates Pulmonary Inflammation and TNF-alpha Release Mediated by Mycoplasma pneumoniae Frontiers in Cellular and Infection Microbiology 2022-03-17 [PMID: 35372108] (Forced Spectroscopy)	Forced Spectroscopy
Munoz-Flores C, Astuya-Villalon A, Romero A, Toledo J Salmonid MyD88 is a key adapter protein that activates innate effector mechanisms through the TLR5M/TLR5S signaling pathway and protects against Piscirickettsia salmonis infection Fish & Shellfish Immunology 2022-01-01 [PMID: 34998987]
Creismeas A, Gazaille C, Bourdon A et al. TRPC3, but not TRPC1, as a good therapeutic target for standalone or complementary treatment of DMD Journal of translational medicine 2021-12-20 [PMID: 34930315]
jiang w, Luo Z, Fitting S et al. Chronic Cannabis Smoking-Enriched Oral Pathobiont Drives Behavioral Changes and Increases beta -Amyloid Protein Production in the Brain EBioMedicine 2021-11-26 [PMID: 34826801]
Firmal P, Shah VK, Pant R, Chattopadhyay S. RING finger protein TOPORS modulates the expression of tumor suppressor SMAR1 in colorectal cancer via the TLR4-TRIF pathway Molecular Oncology 2022-04-01 [PMID: 34689394]
Jacobovitz MR, Rupp S, Voss PA Dinoflagellate symbionts escape vomocytosis by host cell immune suppression Nat Microbiol 2021-04-30 [PMID: 33927382]
Sakai H, Tanaka Y, Tanaka A, Ohdan H TLR-MyD88 signaling blockades inhibit refractory B-1b cell immune responses to transplant-related glycan antigens Am. J. Transplant. 2020-08-31 [PMID: 32865877]
Onishi K, Mochizuki J, Sato A et al. Total RNA and genomic DNA of Lactobacillus gasseri OLL2809 induce interleukin-12 production in the mouse macrophage cell line J774.1 via toll-like receptors 7 and 9 BMC Microbiol. 2020-07-20 [PMID: 32689952] (Mouse)		Mouse
Swaim CD, Canadeo LA, Monte KJ et al. Modulation of Extracellular ISG15 Signaling by Pathogens and Viral Effector Proteins Cell Rep 2020-06-16 [PMID: 32553163] (B/N, Human)	B/N	Human
A study on the correlation of MyD88 expression with gastric cancer. Du Q, Zhu C, Shang Q et al. Int J Clin Exp Pathol [PMID: 31949558] (IF/IHC, WB, Human)	IF/IHC, WB	Human
Jin X, Tian QZ, Zhang M et al. Modulation of SBD-1 expression by Saccharomyces cerevisiae cell wall components in ovine ruminal epithelial cells Res. Vet. Sci. 2020-01-03 [PMID: 31927467]
Zhong H, Chen H, Gu C Sevoflurane Post-treatment Upregulated miR-203 Expression to Attenuate Cerebral Ischemia-Reperfusion-Induced Neuroinflammation by Targeting MyD88 Inflammation 2020-01-02 [PMID: 31897915]
Liu H, Lessieur EM, Saadane A et al. Neutrophil elastase contributes to the pathological vascular permeability characteristic of diabetic retinopathy Diabetologia 2019-10-14 [PMID: 31612267]
Serhan N, Basso L, Sibilano R et al. House dust mites activate nociceptor-mast cell clusters to drive type 2 skin inflammation Nat. Immunol. 2019-10-07 [PMID: 31591569] (Forced Spectroscopy, Mouse)	Forced Spectroscopy	Mouse
Yang Z, Wang L, Yu H et al. Membrane TLR9 Positive Neutrophil Mediated MPLA Protects Against Fatal Bacterial Sepsis Theranostics 2019-08-14 [PMID: 31534550]
Kim Y, Jo M, Schmidt J, et al. Enhanced Potency of GalNAc-conjugated Antisense Oligonucleotides in Hepatocellular Cancer Models Mol Ther 2019-07-16 [PMID: 31303442]
Rathore M, Girard C, Ohanna M et al. Cancer cell-derived long pentraxin 3 (PTX3) promotes melanoma migration through a toll-like receptor 4 (TLR4)/NF-kappa B signaling pathway Oncogene 2019-07-01 [PMID: 31253871] (BindInhib)	BindInhib
Chang-Graham AL, Danhof HA, Engevik MA et al. Wnt5a is a TLR2/4-ligand that induces tolerance in human myeloid cells Commun Biol 2019-05-09 [PMID: 31098409] (In-Cell Western (ICW), Human)	In-Cell Western (ICW)	Human
Wang P, Liu BY, Wu MM et al. Moderate prenatal alcohol exposure suppresses the TLR4-mediated innate immune response in the hippocampus of young rats Neurosci. Lett. 2019-01-30 [PMID: 30710662] (WB, Rat)	WB	Rat
Nguyen TNY, Padungros P, Wongsrisupphakul P et al. Cell wall mannan of Candida krusei mediates dendritic cell apoptosis and orchestrates Th17 polarization via TLR-2/MyD88-dependent pathway. Sci Rep. 2018-11-20 [PMID: 30459422] (B/N, Mouse)	B/N	Mouse
Liu JT, Wu SX, Zhang H et al. Inhibition of MyD88 Signaling Skews Microglia/Macrophage Polarization and Attenuates Neuronal Apoptosis in the Hippocampus After Status Epilepticus in Mice Neurotherapeutics 2018-08-15 [PMID: 30112701] (In Vivo, Mouse)	In Vivo	Mouse
Dishon Shira, Schumacher Adi, Fanous Joseph et al. Development of a Novel Backbone Cyclic Peptide Inhibitor of the Innate Immune TLR/IL1R Signaling Protein MyD88. Scientific Reports 2018-06-21 [PMID: 29930295] (Human)		Human
Park SK, Jin SY, Yeon SH et al. Role of Toll-like receptor 9 signaling on activation of nasal polyp-derived fibroblasts and its association with nasal polypogenesis Int Forum Allergy Rhinol. [PMID: 29901280]
Gorelenkova Miller O, Mieyal JJ. Critical Roles of Glutaredoxin in Brain Cells - Implications for Parkinson's Disease Antioxid. Redox Signal. 2017-11-28 [PMID: 29183158]
Loetsch C, Warren J, Laskowski A et al. Cytosolic Recognition of RNA Drives the Immune Response to Heterologous Erythrocytes Cell Rep 2017-11-07 [PMID: 29117566] (B/N, Mouse)	B/N	Mouse
Dos Santos JC, Grund LZ, Seibert CS et al. Stingray venom activates IL-33 producing cardiomyocytes, but not mast cell, to promote acute neutrophil-mediated injury Sci Rep 2017-08-11 [PMID: 28801624] (B/N, Mouse)	B/N	Mouse
Ho MF, Ingle JN, Bongartz T et al. TCL1A SNPs and estrogen-mediated toll-like receptor-MYD88-dependent NF-kB activation: SNP and SERM-dependent modification of inflammation and immune response. Mol. Pharmacol. 2017-06-14 [PMID: 28615284]
D Yuan, S Huang, E Berger, L Liu, N Gross, F Heinzmann, M Ringelhan, TO Connor, M Stadler, M Meister, J Weber, R Öllinger, N Simonavici, F Reisinger, D Hartmann, R Meyer, M Reich, M Seehawer, V Leone, B Höchst, D Wohlleber, S Jörs, M Prinz, D Spalding, U Protzer, T Luedde, L Terraccian, M Matter, T Longerich, P Knolle, T Ried, V Keitel, F Geisler, K Unger, E Cinnamon, E Pikarsky, N Hüser, RJ Davis, DF Tschaharga, R Rad, A Weber, L Zender, D Haller, M Heikenwald Kupffer Cell-Derived Tnf Triggers Cholangiocellular Tumorigenesis through JNK due to Chronic Mitochondrial Dysfunction and ROS Cancer Cell, 2017-06-12;31(6):771-789.e6. 2017-06-12 [PMID: 28609656]
Liu F, Wang Z, Qiu Y et al. Suppression of MyD88-dependent signaling alleviates neuropathic pain induced by peripheral nerve injury in the rat J Neuroinflammation. 2017-03-31 [PMID: 28359290] (In-Cell Western (ICW), Rat)	In-Cell Western (ICW)	Rat
Conant K, Daniele S, Bozzelli PL et al. Matrix metalloproteinase activity stimulates N-cadherin shedding and the soluble N-cadherin ectodomain promotes classical microglial activation. J Neuroinflammation. 2017-03-17 [PMID: 28302163] (BindInhib, Rat)	BindInhib	Rat
Hsieh LT, Nastase MV, Roedig H et al. Biglycan- and Sphingosine Kinase-1 Signaling Crosstalk Regulates the Synthesis of Macrophage Chemoattractants. Int J Mol Sci. 2017-03-09 [PMID: 28282921] (IF/IHC, Mouse)	IF/IHC	Mouse
Hirai M, Kitahara H, Kobayashi Y et al. Regulation of PD-L1 expression in a high-grade invasive human oral squamous cell carcinoma microenvironment. Int. J. Oncol. 2017-01-01 [PMID: 27922697] (ELISA, Human)	ELISA	Human
Dreschers S, Saupp P, Hornef M et al. Reduced PICD in Monocytes Mounts Altered Neonate Immune Response to Candida albicans. PLoS ONE. 2016-11-21 [PMID: 27870876] (FLOW, Yeast)	FLOW	Yeast
Leaf IA, Nakagawa S, Johnson BG et al. Pericyte MyD88 and IRAK4 control inflammatory and fibrotic responses to tissue injury. J. Clin. Invest. 2016-11-21 [PMID: 27869651]
Ke X, Zhang S, Wu M et al. Tumor-associated macrophages promote invasion via Toll-like receptors signaling in patients with ovarian cancer. Int. Immunopharmacol. 2016-09-05 [PMID: 27608303] (B/N)	B/N
Du L, Liu Y, Du Y et al. Porcine reproductive and respiratory syndrome virus (PRRSV) up-regulates IL-15 through PKCb1-TAK1-NF-kB signaling pathway. Virology 2016-06-15 [PMID: 27318153] (In Vivo, Porcine)	In Vivo	Porcine
Fan H, Zhang K, Shan L et al. Reactive astrocytes undergo M1 microglia/macrohpages-induced necroptosis in spinal cord injury. Mol Neurodegener 2016-02-03 [PMID: 26842216] (WB)	WB
Hsieh LT, Frey H, Nastase MV et al. Bimodal role of NADPH oxidases in the regulation of biglycan-triggered IL-1b synthesis. Matrix Biol. 2015-12-12 [PMID: 26689330] (In vitro)	In vitro
Seifert L, Deutsch M, Alothman S, Alqunaibit D. Dectin-1 Regulates Hepatic Fibrosis and Hepatocarcinogenesis by Suppressing TLR4 Signaling Pathways. Cell Reports. 2015-12-01 [PMID: 26655905] (Mouse)		Mouse
Jung YJ, Lee AS, Nguyen-Thanh T et al. Hyaluronan-induced VEGF-C promotes fibrosis-induced lymphangiogenesis via Toll-like receptor 4-dependent signal pathway. Biochem. Biophys. Res. Commun. 2015-09-08 [PMID: 26362177]
Yuan C, Zheng Y, Zhang B et al. Thymosin a1 promotes the activation of myeloid-derived suppressor cells in a Lewis lung cancer model by upregulating Arginase 1 Biochem. Biophys. Res. Commun. 2015-06-23 [PMID: 26111447] (Func, B/N, Mouse)	Func, B/N	Mouse
Roh YS, Zhang B, Loomba R, Seki E. TLR2 and TLR9 contribute to alcohol-mediated liver injury through induction of CXCL1 and neutrophil infiltration. Am. J. Physiol. Gastrointest. Liver Physiol. 2015-04-30 [PMID: 25930080] (In Vivo)	In Vivo
Wang C, Wang H, Chang DY et al. High mobility group box 1 contributes to anti-neutrophil cytoplasmic antibody-induced neutrophils activation through receptor for advanced glycation end products (RAGE) and Toll-like receptor 4 Arthritis Res. Ther. 2015-04-19 [PMID: 25889374] (B/N)	B/N
Murshid A, Gong J, Prince T et al. Scavenger Receptor SREC-I Mediated Entry of TLR4 into Lipid Microdomains and Triggered Inflammatory Cytokine Release in RAW 264.7 Cells upon LPS Activation PLoS ONE 2015-04-03 [PMID: 25836976] (B/N)	B/N
Lin X, Kong J, Wu Q et al. Effect of TLR4/MyD88 Signaling Pathway on Expression of IL-1 and TNF-in Synovial Fibroblasts from Temporomandibular Joint Exposed to Lipopolysaccharide Mediators of Inflammation. 2015-03-03 [PMID: 25810567] (In vitro, Rat)	In vitro	Rat
Perez-Yepez EA, Ayala-Sumuano JT, Lezama R, Meza I. A novel b-catenin signaling pathway activated by IL-1b leads to the onset of epithelial-mesenchymal transition in breast cancer cells. Cancer Lett. 2014-08-15 [PMID: 25135221] (B/N, Human) Details: MyD88 Inhibitor Peptide Set (IMG-2005) used/incubated for 24 h at 100 uM concentration for blocking IL-1RI signaling in MCF-7 and MCF-7 6D cells that were stimulated or not with IL1 Beta (Fig. 2B, D ).	B/N	Human
Benedetti F, Davinelli S, Krishnan S et al. Sulfur compounds block MCP-1 production by Mycoplasma fermentans-infected macrophages through NF-kB inhibition. J Transl Med. 2014-06-06 [PMID: 24886588] (In vitro, B/N, Human) Details: U937 cells, Fig 5B. 75 uM MyD88 inhibitory or control peptide was added to cell cultures 1 hr before Mycoplasma Ferementans Infection. Peptides were added daily and cell supernatants were collected at 48 h and tested for MCP-1 production. Readout system:	In vitro, B/N	Human
Duluc D, Joo H, Ni L et al. Induction and Activation of Human Th17 by Targeting Antigens to Dendritic Cells via Dectin-1. J. Immunol. 2014-05-16 [PMID: 24835401] (In vitro, B/N, Human) Details: PBMC, Fig 6C. The MyD88 inhibitory peptide blocked RORC expression. Readout system: RT-PCR analysis of Dectn-1 induced RORC expression.	In vitro, B/N	Human
Li Y, Zhang H, Zhang H et al. Toll-like receptor 4 signaling contributes to paclitaxel-induced peripheral neuropathy. J Pain 2014-04-19 [PMID: 24755282] (In Vivo, Forced Spectroscopy, Rat) Details: Spinal cord: WB (Figs 1C, 2A, 8A), MyD88 was detected at ~31kDa	In Vivo, Forced Spectroscopy	Rat
Ge Y, Chen J, Qiu X et al. Natural killer cell intrinsic toll-like receptor MyD88 signaling contributes to IL-12-dependent IFN-gamma production by mice during infection with Toxoplasma gondii. Int. J. Parasitol. 2014-04-13 [PMID: 24727091] (Forced Spectroscopy, Mouse) Details: Freshly isolated Nk natural killer cells, Fig 4A. Nk cells were preincubated with 100 um the MyD88 or control peptide for 24 h at 37 degrees C and then stimulated with T. gondi antigens or TNF-alpha plus IL-12 for 6 hr and then analyzed for IFN-gamma expr	Forced Spectroscopy	Mouse
Bermbach S, Weinhold K, Roeder T et al. Mechanisms of Cilia-Driven Transport in the Airways in the Absence of Mucus. Am. J. Respir. Cell Mol. Biol. 2014-01-27 [PMID: 24467665] (In vitro, Mouse) Details: Explanted mouse trachae stimulated with LPS or H influenzae, Figs SE1B, 7B-C. Pre-incubation with the MyD88 inhibitory peptide but not the control peptide prior to LPS stimulation prevented LPS-induced nuclear translocation of NF-kB (Fig 7B-C). Readout sy	In vitro	Mouse
Chandel HS, Pandey SP, Shukla D et al. TLRs and CD40 modulate each others expression affecting Leishmania major infection. Clin. Exp. Immunol. 2014-01-06 [PMID: 24387292] (In vitro, Mouse) Details: Mouse macrophages, Fig 2b. Mouse macrophages were pretreated with the MyD88 inhibitory peptide or control peptide for 1 hr followed by treatment with various ligands. The inhibitory, but not the control peptide impaired CD40 expression as measured by RNA	In vitro	Mouse
Ostrovsky O, Shimoni A, Baryakh P et al. Modification of heparanase gene expression in response to conditioning and LPS treatment: strong correlation to rs4693608 SNP. J Leukoc Biol 2013-12-06 [PMID: 24319286] (Forced Spectroscopy)	Forced Spectroscopy
Spadaro M, Montone M, Arigoni M et al. Recombinant human lactoferrin induces human and mouse dendritic cell maturation via Toll-like receptors 2 and 4. FASEB J. 2014-01-01 [PMID: 24088817] (B/N, Human) Details: Dendritic cell precursors isolated from human PBMC, inhibitory and control peptides were used at 150 uM: Fig 4. Pre-incubation with the inhibitor but not the control peptide reduced the ability of talactoferrin (TLF) to induce the expression of maturation	B/N	Human
Dubbert J, Bowers A, Su Y, McClenahan D. Effect of TRIF on permeability and apoptosis in bovine microvascular endothelial cells exposed to lipopolysaccharide. Vet J 2013-11-01 [PMID: 24075204] (Func, Bovine)	Func	Bovine
Conti L, Lanzardo S, Arigoni M, et al. The noninflammatory role of high mobility group box 1/Toll-like receptor 2 axis in the self-renewal of mammary cancer stem cells. FASEB J 2013-12-01 [PMID: 23970797] (Forced Spectroscopy, Mouse)	Forced Spectroscopy	Mouse
Yang G, Zhou Y, Liu X et al. A mutation in MYD88 (L265P) supports the survival of lymphoplasmacytic cells by activation of Bruton tyrosine kinase in Waldenstrom macroglobulinemia. Blood. 2013-08-15 [PMID: 23836557]
Karlsen TA, Brinchmann JE. Liposome delivery of microRNA-145 to mesenchymal stem cells leads to immunological off-target effects mediated by RIG-I. Mol Ther. 2013-06-01 [PMID: 23568258]
Ayala-Sumuano Jorge-Tonatiuh, Tellez-Lopez Victor M, Dominguez-Robles M del Carmen et al. Toll-like receptor signaling activation by Entamoeba histolytica induces beta defensin 2 in human colonic epithelial cells: its possible role as an element of the innate immune response. PLoS Negl Trop Dis. 2013-01-01 [PMID: 23469306] (ELISA)	ELISA
Turco F, Sarnelli G, Cirillo C et al. Enteroglial-derived S100B protein integrates bacteria-induced Toll-like receptor signalling in human enteric glial cells. Gut. [PMID: 23292665] Details: Citation using the Alexa Fluor 488 form of this antibody.
Ciechomska M, Huigens CA, Hugle T et al. Toll-like receptor-mediated, enhanced production of profibrotic TIMP-1 in monocytes from patients with systemic sclerosis: role of serum factors. Ann Rheum Dis. 2013-08-01 [PMID: 23223421]
Treon SP, Xu L, Yang G et al. MYD88 L265P somatic mutation in Waldenstr0m's macroglobulinemia. N Engl J Med. 2012-08-30 [PMID: 22931316]
Abdulkhalek S, Guo M, Amith SR et al. G-protein coupled receptor agonists mediate Neu1 sialidase and matrix metalloproteinase-9 cross-talk to induce transactivation of TOLL-like receptors and cellular signaling. Cell Signal. 2012-11-01 [PMID: 22759791]
Newkirk MM, Mitchell S, Procino M et al. Chronic smoke exposure induces rheumatoid factor and anti-heat shock protein 70 autoantibodies in susceptible mice and humans with lung disease. Eur J Immunol. 2012-04-01 [PMID: 22531929]
Ruscanu S, Pascale F, Bourge M et al. The double-stranded RNA bluetongue virus induces type I interferon in plasmacytoid dendritic cells via a MYD88-dependent TLR7/8-independent signaling pathway. J Virol. 2012-05-01 [PMID: 22438548]
Hovden AO, Karlsen M, Jonsson R, Appel S. The bacterial preparation OK432 induces IL-12p70 secretion in human dendritic cells in a TLR3 dependent manner. PLoS One. 2012-01-01 [PMID: 22363584]
Saha S, Bhanja P, Liu L et al. TLR9 agonist protects mice from radiation-induced gastrointestinal syndrome. PLoS One. 2012-01-01 [PMID: 22238604]
Galvan-Moroyoqui Jose Manuel, Del Carmen Dominguez-Robles M, Meza Isaura. Pathogenic bacteria prime the induction of Toll-like receptor signalling in human colonic cells by the Gal/GalNAc lectin Carbohydrate Recognition Domain of Entamoeba histolytica. Int J Parasitol. 2011-08-15 [PMID: 21787776]
Iwakiri Yasuko. Endothelial dysfunction in the regulation of cirrhosis and portal hypertension. Liver Int. 2012-02-01 [PMID: 21745318] (Human)		Human
Pearl JI, Ma T, Irani AR et al. Role of the Toll-like receptor pathway in the recognition of orthopedic implant wear-debris particles. Biomaterials. 2011-08-01 [PMID: 21592562]
Giangreco A, Lu L, Mazzatti DJ et al. Myd88 deficiency influences murine tracheal epithelial metaplasia and submucosal gland abundance. J Pathol. 2011-06-01 [PMID: 21557220]
Vohradska Eliska, Vohradsky Jiri. Virtual mutagenesis of the yeast cyclins genetic network reveals complex dynamics of transcriptional control networks. PLoS One. 2011-01-01 [PMID: 21541341]
Brown GT, McIntyre TM. Lipopolysaccharide signaling without a nucleus: kinase cascades stimulate platelet shedding of proinflammatory IL-1B-rich microparticles. J Immunol. 2011-05-01 [PMID: 21430222] Details: TIRAP (IMG-2006-1), MyD88 (IMG-2005), and TRAF6 (IMG-2002) Inhibitory Peptide Sets. FA: Human and mouse blood platelets treated with or without LPS, soluble CD14, inhibitory peptides, and LBP. Readout assay: real time-PCR, Fig 2 & 6.
Siednienko J, Gajanayake T, Fitzgerald KA et al. Absence of MyD88 results in enhanced TLR3-dependent phosphorylation of IRF3 and increased IFN-B and RANTES production. J Immunol. 2011-02-15 [PMID: 21248248]
Jagavelu Kumaravelu, Routray Chittaranjan, Shergill Uday et al. Endothelial cell toll-like receptor 4 regulates fibrosis-associated angiogenesis in the liver. Hepatology. 2010-08-01 [PMID: 20564354]
Lindgren A, Pavlovic V, Flach CF et al. Interferon-gamma secretion is induced in IL-12 stimulated human NK cells by recognition of Helicobacter pylori or TLR2 ligands. Innate Immun. 2011-04-01 [PMID: 20130107]
Ma J, Wang J, Wan J et al. Morphine disrupts interleukin-23 (IL-23)/IL-17-mediated pulmonary mucosal host defense against Streptococcus pneumoniae infection. Infect Immun. 2010-02-01 [PMID: 19995896]
Ogawa Y, Kawamura T, Kimura T et al. Gram-positive bacteria enhance HIV-1 susceptibility in Langerhans cells, but not in dendritic cells, via Toll-like receptor activation. Blood. 2009-05-21 [PMID: 19279330]
Mogensen TH, Berg RS, Ostergaard L, Paludan SR. Streptococcus pneumoniae stabilizes tumor necrosis factor alpha mRNA through a pathway dependent on p38 MAPK but independent of Toll-like receptors. BMC Immunol. 2008-09-16 [PMID: 18796140]
Scott MJ, Billiar TR. Beta2-integrin-induced p38 MAPK activation is a key mediator in the CD14/TLR4/MD2-dependent uptake of lipopolysaccharide by hepatocytes. J Biol Chem. 2008-10-24 [PMID: 18701460] Details: 1. MyD88 (IMG-2005): MyD88 peptide inhibition (mouse WT hepatocytes), Figs. 4A,D. 2. TIRAP (IMG-2006): TIRAP peptide inhibition (mouse WT hepatocytes), Figs. 4B,D; 5B; 6E.
Funderburg N, Lederman MM, Feng Z et al. Human -defensin-3 activates professional antigen-presenting cells via Toll-like receptors 1 and 2. Proc Natl Acad Sci U S A. 2007-11-20 [PMID: 18006661] (Human) Details: IMG-2005 (Human C14+ cells): Fig. 2 B,C.		Human
Mogensen TH, Berg RS, Paludan SR, Ostergaard L. Mechanisms of dexamethasone-mediated inhibition of Toll-like receptor signaling induced by Neisseria meningitidis and Streptococcus pneumoniae. Infect Immun. 2008-01-01 [PMID: 17938219] (Human) Details: IMG(2005) (human PBMC and THP-1 cell line): Fig. 2A,B.		Human
Ahmad R, Sylvester J, Zafarullah M. MyD88, IRAK1 and TRAF6 knockdown in human chondrocytes inhibits interleukin-1-induced matrix metalloproteinase-13 gene expression and promoter activity by impairing MAP kinase activation. Cell Signal. 2007-12-01 [PMID: 17905570] (WB, Human) Details: IMG-2005 (human knee articular chondrocytes): Figs. 1A-D; 2C,E. Readout assays to measure the effect of MyD88 inhibition included RT-PCR (MMP-13 levels), WB (MMP-13 levels and levels of p38, ERK1/2, JNK, Jun phosphorylation), AP-1 DNA-binding activity act	WB	Human
Rolls A, Shechter R, London A et al. Toll-like receptors modulate adult hippocampal neurogenesis. Nat Cell Biol. 2007-09-01 [PMID: 17704767]
Fan J, Li Y, Levy RM et al. Hemorrhagic shock induces NAD(P)H oxidase activation in neutrophils: role of HMGB1-TLR4 signaling. J Immunol. 2007-05-15 [PMID: 17475888] Details: IMG-2005: Fig 5, Fig 6C and D.
Uto T, Wang X, Sato K et al. Targeting of antigen to dendritic cells with poly(gamma-glutamic acid) nanoparticles induces antigen-specific humoral and cellular immunity. J Immunol. 2007-03-01 [PMID: 17312143]
Mogensen TH, Paludan SR, Kilian M, Ostergaard L. Two neisseria meningitidis strains with different ability to stimulate toll-like receptor 4 through the MyD88-independent pathway. Scand J Immunol. 2006-12-01 [PMID: 17083621] Details: IMG-2005 (HEK293 cells): Fig. 6A,B.
Bamford S, Ryley H, Jackson SK. Highly purified lipopolysaccharides from Burkholderia cepacia complex clinical isolates induce inflammatory cytokine responses via TLR4-mediated MAPK signalling pathways and activation of NFkappaB. Cell Microbiol. 2007-02-01 [PMID: 17002785] Details: IMG-2005 (MM6 cells): Fig. 4A-C.
Abel K, Wang Y, Fritts L et al. Deoxycytidyl-deoxyguanosine oligonucleotide classes A, B, and C induce distinct cytokine gene expression patterns in rhesus monkey peripheral blood mononuclear cells and distinct alpha interferon responses in TLR9-expressing rhesus monkey plasmacytoid den Clin Diagn Lab Immunol. 2005-05-01 [PMID: 15879022] Details: IMG-305C [Flow (Intracellular), Fig. 5] on Rhesus monkey spleen cell suspensions and PBMC.
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Reviews for MyD88 Inhibitor (NBP2-29328) (3) 53

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5 reviewed by: Adrienne Franks	05/12/2017	View
5 reviewed by: Kyong-su Park	04/28/2017	View
5 reviewed by: Adrienne Franks	04/19/2017	View

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FAQs for MyD88 Inhibitor (NBP2-29328). (Showing 1 - 4 of 4 FAQs).

I have a question regarding the product NBP2-29328 and want to know how the inhibitory peptide passes through the cell wall?
- The MyD88 inhibitory peptide contains a protein transduction (PTD) sequence derived from antennapedia which renders the PTD sequence renders the peptide cell permeable. The antennapedia technology was first described by Derossi et al, The third helix of the Antennapedia homeodomain translocates through biological membranes and is used in a number of our inhibitory peptides. The MyD88 inhibitory peptide also contains a sequence from the MyD88 TIR homodimerization domain (Loiarro et al, Peptide-mediated interference of TIR domain dimerization in MyD88 inhibits interleukin-1-dependent activation of NF-{kappa}B). The control peptide consists of only the PTD sequence. The Derossi and Loiarro technology references are attached for additional background information. Please also follow this link to representative publications citing the MyD88 inhibitory peptide.
I'm looking into purchasing NBP2-29328 for use and I am wondering if you can give me more information. Specifically, I would like to inject this drug ICM (directly into the CNS) using a rat model, and I am wondering what information you can provide about
- Unfortunately, I do not see any in-vivo data for this product on our internal records. Most of the information that we have on our records for this product may be seen at its online datasheets
I was wondering whether NBP2-29328-5mg has biological activity.
- Our MyD88 Inhibitor Peptide Set does have biological activity.
Is this the same as ST2825 compound described by Loiarro et al, 2007?
- ST2825 is the heptapeptide only, whereas the inhibitor peptide from NBP2-29328 corresponds to the association between the hexapeptide + the PTD sequence from antennapedia. In that case, this would suggest that the product used in Loiarro et al. is not the same as NBP2-29328.

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Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ... Read full blog post.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta. STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-... Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts... Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation. In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy... Read full blog post.

MYD88 Expression and Tumorigenesis
MyD88, also called myeloid differentiation primary response gene 88, encodes a cytosolic adapter protein that plays an essential role in innate and adaptive immune responses. The innate immune system recognizes the presence of bacterial pathogens thro... Read full blog post.

TLR7 and Immune Response Regulation
Toll-like receptor 7 (TLR7) is a protein encoded by the TLR7 gene in humans and is a member of TLR family. TLRs controls host immune response against pathogens (e.g. viruses, bacteria and fungi) through recognition of pathogen-associated molecular pat... Read full blog post.

MYD88: Fanning Inflammation and Immune Responses
Myeloid differentiation primary response gene 88 (MYD88) encodes a cytosolic adapter protein that plays an essential role in innate and adaptive immune responses. MYD88 protein functions as an essential signal transducer in the interleukin-1 and in To... Read full blog post.

MyD88 Antibodies for IL Signaling and Immunity Research
The myeloid differentiation protein MyD88 (myeloid differentiation primary response protein) was originally identified and characterized as a primary upregulated response gene in interleukin-6 mediated myeloid differentiation. Now, MyD88 is known to b... Read full blog post.

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	Adrienne Franks 05/12/2017
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	Kyong-su Park 04/28/2017
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MYD88

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

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Reviews for MyD88 Inhibitor (NBP2-29328) (3) 53

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