|
|
|
|
Simple Western™ separates proteins by size, and precisely manages immunoprobing, incubations, washes and even detection steps by removing the manual, error-prone steps in standard Western blots. Over 1,300 Novus Biologicals and R&D Systems antibodies have been certified on ProteinSimple's Simple Western platforms. View our complete portfolio of Simple Western validated antibodies. If your antibody hasn’t been validated for Simple Western, you can submit your results to our Innovator's Reward Program for a voucher for the full purchase price for Risk-Free Testing. If your antibody of choice has not yet been screened in Simple Western, Novus Biologicals will reward you for being the first to test the product in this application. Learn more about Novus Rewards and their benefits. The Simple Western Antibody Database on Bio-Techne.com is a user-interactive listing of all Simple Western validated antibodies to date. Submit your antibody validation data and get a free Separation or Detection Module! |
|
Accelerate your assay development on Simple Western by learning from the experts! | |
 | In a series of live workshops, our field application scientists will share their tips, tricks, and best practices on Simple Western to improve your assays from start to finish. You will learn how to efficiently optimize assays across the development pipeline, accurately analyze data using Compass software, and more. Join us in the Bio-Techne Academy, our first workshop begins on January 26th, 2023!
|
Example data from traditional Western blot versus Simple Western |
|
Traditional Western Blots |
Simple Western |
||
 |
Detection of Human cIAP 1/HIAP 2 by Western Blot. HEK293 human embryonic kidney cell line mock transfected or transfected with full length human cIAP-1. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human cIAP‑1/HIAP‑2 Monoclonal Antibody (Catalog # MAB818) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for cIAP‑1/HIAP‑2 at approximately 72 kDa. |
 |
Detection of Human cIAP 1/HIAP 2 by Simple Western. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and cIAP‑1/HIAP‑2 knockout HeLa cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for cIAP‑1/HIAP‑2 at approximately 66 kDa (as indicated) in the parental HeLa cell line but is not detectable in knockout HeLa cell line. Mouse Anti-Human cIAP‑1/HIAP‑2 Monoclonal Antibody (Catalog # MAB818) was used at 5 µg/mL. This experiment was conducted under reducing conditions with the 12-230 kDa separation system.  |
 |
Detection of Human/Mouse/Rat HSP60 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF 7 human breast cancer cell line, NIH 3T3 mouse embryonic fibroblast cell line, and Nb2 11 rat lymphoma cell line. A specific band was detected for HSP60 (Catalog # AF1800, R&D Systems) at approximately 62 kDa (as indicated). See full datasheet. |
 |
Detection of Human and Mouse HSP60 by Simple Western. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and NIH 3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP60 (Catalog # AF1800, R&D Systems) at approximately 60 kDa (as indicated).This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. See full datasheet. |
