TLR7 Antibody - BSA Free

Toll-like receptor 7 (TLR7) is a type I transmembrane protein expressed on the surface of endosomes and has a role in pathogen-associated molecular patterns (PAMPs) recognition and host defense (1-3). TLR7 is primarily expressed in the brain, placenta, spleen, stomach, and lungs (4). TLR7 recognizes microbial single stranded RNA (ssRNA), specifically guanosine and its derivatives (1-3). Human TLR7 cDNA encodes a 1049 amino acid (aa) protein with a theoretical molecular weight (MW) of 120.9 kDa (4). The TLR7 protein consists of a signal sequence, an 813 aa extracellular domain containing leucine-rich repeats (LRRs) which form a horseshoe-like shape, a 21 aa transmembrane domain, and a 189 aa cytoplasmic domain with cytosolic Toll-interleukin-1 receptor homology (TIR) domains (1,2,4). TLR7 and its fellow subfamily members, TLR8 and TLR9, possess a characteristic Z-loop between two LRRs with proteolytic Z-loop processing required for TLR activation (2). Z-loop cleavage in TLR7 allows for guanosine and uridine-rich ssRNA binding to the 1st and 2nd ligand binding site, respectively (2). The TIR domain associates with the adaptor protein myeloid differentiation primary response protein (MyD88) to initiate downstream signaling (1-3,5,6). Following activation by PAMPs, TLR7 dimerizes and bound MyD88 interacts with interleukin-1 receptor-associated kinase-4 (IRAK-4) (1,5). Together the complex recruits IRAK-1 and IRAK-2, which become phosphorylated, and interact with tumor necrosis factor receptor-associated factor 6 (TRAF6) (1,5). TRAF6 induces the activation of mitogen-activated protein kinase (MAPK), nuclear factor-kappaB (NF-kappaB), and interferon-regulatory factor 7 (IRF7), leading to interferon production and pro-inflammatory cytokine secretion associated with immune response (1,5).

While TLRs play an important role in innate immune response, dysfunction in the TLR-MyD88 signaling cascade has also been reported in various autoimmune disorders (5,6). Elevated expression of TLR7 is associated with increased risk of system lupus erythematosus (SLE), an autoimmune disease involving B cell hyperactivity (6,7). Studies involving mouse models has also found that increased TLR7 expression predisposes mice to a lupus-like disease (7). Therapeutics targeting TLR7 have been developed to either enhance or inhibit its activity depending on the circumstance. For example, TLR7 agonists such as imiquimod, resiquimod, and 852A are used to increase TLR7 activity for treatment of cancers and to fight viral infections (7,8). On the other hand, TLR7 antagonists inhibit its activation and have been developed to combat chronic immune stimulation as seen in inflammatory and autoimmune diseases (8).

References

1. Petes C, Odoardi N, Gee K. The Toll for Trafficking: Toll-Like Receptor 7 Delivery to the Endosome. Front Immunol. 2017;8:1075. https://doi.org/10.3389/fimmu.2017.01075

2. Maeda K, Akira S. TLR7 Structure: Cut in Z-Loop. Immunity. 2016;45(4):705-707. https://doi.org/10.1016/j.immuni.2016.10.003

3. Krieg AM, Vollmer J. Toll-like receptors 7, 8, and 9: linking innate immunity to autoimmunity. Immunol Rev. 2007;220:251-269. https://doi.org/10.1111/j.1600-065X.2007.00572.x

4. Uniprot (Q9NYK1)

5. Zheng C, Chen J, Chu F, Zhu J, Jin T. Inflammatory Role of TLR-MyD88 Signaling in Multiple Sclerosis. Front Mol Neurosci. 2020;12:314. https://doi.org/10.3389/fnmol.2019.00314

6. Chi H, Li C, Zhao FS, et al. Anti-tumor Activity of Toll-Like Receptor 7 Agonists. Front Pharmacol. 2017;8:304. https://doi.org/10.3389/fphar.2017.00304

7. Fillatreau S, Manfroi B, Dorner T. Toll-like receptor signalling in B cells during systemic lupus erythematosus. Nat Rev Rheumatol. 2021;17(2):98-108. https://doi.org/10.1038/s41584-020-00544-4

8. Patinote C, Karroum NB, Moarbess G, et al. Agonist and antagonist ligands of toll-like receptors 7 and 8: Ingenious tools for therapeutic purposes. Eur J Med Chem. 2020;193:112238. https://doi.org/10.1016/j.ejmech.2020.112238

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 15 applications: BA, Block/Neutralize, Cytometric Bead Assay Standard, FLOW, Flow Cytometry Control, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IP, Immunoprecipitation, PLA, WB, Western Blot.

Showing Publications 1 - 10 of 80. Show All 80 Publications. Collapse Publications.

Publications using NBP2-24906	Applications	Species
Liu G, Haw TJ, Starkey MR et al. TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase Nature communications 2023-11-14 [PMID: 37963864] (WB, IHC, Mouse) Details: IB Dilution 1:2000; IHC Dilution 1:100	WB, IHC	Mouse
Deng L, Gao R, Chen H et al. Let-7b-TLR7 Signaling Axis Contributes to the Anesthesia/Surgery-Induced Cognitive Impairment Molecular neurobiology 2023-10-02 [PMID: 37782443]
Liu J, Ke P, Guo H et al. Activation of TLR7-mediated autophagy increases epileptic susceptibility via reduced KIF5A-dependent GABAA receptor transport in a murine model Experimental & molecular medicine 2023-06-01 [PMID: 37258573] (IHC, WB, Mouse)	IHC, WB	Mouse
Caccuri F, Messali S, Bortolotti D et al. Competition for dominance within replicating quasispecies during prolonged SARS-CoV-2 infection in an immunocompromised host Virus Evolution 2022-06-14 [PMID: 35706980] (Immunoprecipitation, Western Blot, Block/Neutralize)	Immunoprecipitation, Western Blot, Block/Neutralize
Hayakawa K, Fujishiro M, Yoshida Y et al. Exposure of female NZBWF1 mice to imiquimod induced lupus nephritis at an early age via a unique mechanism that differed from spontaneous onset Clinical and Experimental Immunology 2022-02-02 [PMID: 35260898] (WB)	WB
Marie Rouanet, Naima Hanoun, Hubert Lulka, Cindy Ferreira, Pierre Garcin, Martin Sramek, Godefroy Jacquemin, Agnès Coste, Delphine Pagan, Carine Valle, Emeline Sarot, Vera Pancaldi, Frédéric Lopez, Louis Buscail, Pierre Cordelier The antitumoral activity of TLR7 ligands is corrupted by the microenvironment of pancreatic tumors. Molecular therapy : the journal of the American Society of Gene Therapy 2022-04-11 [PMID: 35038581]
Zhu Y, Wu Z, Yan W et al. Allosteric inhibition of SHP2 uncovers aberrant TLR7 trafficking in aggravating psoriasis EMBO molecular medicine 2021-12-22 [PMID: 34936223] (IHC-P, ICC/IF, Mouse, Human)	IHC-P, ICC/IF	Mouse, Human
Branchi V, Esser L, Boden C et al. A Combined TLR7/TLR9/GATA3 Score Can Predict Prognosis in Biliary Tract Cancer Diagnostics (Basel, Switzerland) 2021-09-01 [PMID: 34573939] (IF/IHC, Human)	IF/IHC	Human
Proskocil BJ, Wai K, Lebold KM et al. TLR7 is expressed by support cells, but not sensory neurons, in ganglia Journal of neuroinflammation 2021-09-16 [PMID: 34530852] (IHC-P, Mouse)	IHC-P	Mouse
Zhang L, Luo H, Meng W et al. Integration of flow cytometry and computational analysis to dissect the epidermal cellular subsets in keloids that correlate with recurrence The Journal of investigative dermatology 2021-04-08 [PMID: 33839145] (IF/IHC)	IF/IHC
Qin L, Zou J, Barnett A et al. TRAIL Mediates Neuronal Death in AUD: A Link between Neuroinflammation and Neurodegeneration International Journal of Molecular Sciences 2021-03-04 [PMID: 33806288] (WB, IF/IHC, Human)	WB, IF/IHC	Human
Huang YL, Huang MT, Sung PS et al. Endosomal TLR3 co-receptor CLEC18A enhances host immune response to viral infection Communications biology 2021-02-18 [PMID: 33603190] (ICC/IF, Mouse, Human)	ICC/IF	Mouse, Human
Zhang L, Cen Y, Huang Q et al. Computational Flow Cytometric Analysis to Detect Epidermal Subpopulations In Human Skin Biomed Eng Online 2021-02-18 [PMID: 33596908]
Agier J, Brzezinska-Blaszczyk E, Witczak P et al. The impact of TLR7 agonist R848 treatment on mast cell phenotype and activity Cellular Immunology 2020-10-01 [PMID: 33158544] (FLOW, WB, Rat)	FLOW, WB	Rat
Karadimou G, Plunde O, Pawelzik SC et al. TLR7 Expression Is Associated with M2 Macrophage Subset in Calcific Aortic Valve Stenosis Cells 2020-07-16 [PMID: 32708790] (ICC/IF, Human)	ICC/IF	Human
Hoshikawa N, Sakai A et al. Targeting Extracellular miR-21-TLR7 Signaling Provides Long-Lasting Analgesia in Osteoarthritis. Mol Ther Nucleic Acids 2020-06-03 [PMID: 31841992] (ICC/IF, Rat)	ICC/IF	Rat
Alshammari TK, Alghamdi H, Green TA et al. Assessing the role of toll-like receptor in isolated, standard and enriched housing conditions PLoS ONE 2019-10-24 [PMID: 31647818] (ICC/IF, Mouse)	ICC/IF	Mouse
Gu R, Huang T, Xiao J et al. The IRE1 alpha Arm of UPR Regulates Muscle Cells Immune Characters by Restraining p38 MAPK Activation Front Physiol 2019-09-19 [PMID: 31607947] (WB, Mouse)	WB	Mouse
Huang Y, Zhou F, Xiao Y et al. TLR7 mediates increased vulnerability to ischemic acute kidney injury in diabetes Rev Assoc Med Bras (1992) 2019-09-12 [PMID: 31531603] (WB, Human)	WB	Human
Kasurinen A, Hagstrom J, Laitinen A et al. Evaluation of toll-like receptors as prognostic biomarkers in gastric cancer: high tissue TLR5 predicts a better outcome Sci Rep [PMID: 31467388] (IHC-P, Human)	IHC-P	Human
Lanki M, Seppanen H, Mustonen H et al. Toll-like receptor 1 predicts favorable prognosis in pancreatic cancer PLoS ONE 2019-07-17 [PMID: 31314777] (IF/IHC, Human)	IF/IHC	Human
Ruuskanen M, Leivo I, Minn H et al. Expression of toll-like receptors in non-endemic nasopharyngeal carcinoma BMC Cancer 2019-06-25 [PMID: 31238894] (IHC-P, Human)	IHC-P	Human
Haeggblom L, Nasman A, Ramqvist T et al. TLR5 and TLR7 are differentially expressed in human papillomavirus-positive and negative base of tongue squamous cell carcinoma, and TLR7 may have an independent prognostic influence Acta Otolaryngol. 2019-02-22 [PMID: 30794027] (IHC-P, Human)	IHC-P	Human
Kapelouzou A, Kontogiannis C, Tsilimigras DI et al. Differential expression patterns of Toll Like Receptors and Interleukin-37 between calcific aortic and mitral valve cusps in humans Cytokine 2019-04-01 [PMID: 30716659] (IF/IHC, Human)	IF/IHC	Human
Blanchard EL, Loomis KH, Bhosle SM et al. Proximity ligation assays for in situ detection of innate immune activation: focus on in vitro transcribed mRNA. Mol Ther Nucleic Acids. [PMID: 30579042] (PLA, Mouse)	PLA	Mouse
Sakata Kei, Nakayamada Shingo, Miyazaki Yusuke et al. Up-Regulation of TLR7-Mediated IFN-alpha Production by Plasmacytoid Dendritic Cells in Patients With Systemic Lupus Erythematosus. Frontiers in Immunology 2018-08-28 [PMID: 30210502] (ICC/IF, Human)	ICC/IF	Human
Loomis KH, Lindsay KE, Zurla C et al. In Vitro Transcribed mRNA Vaccines with Programmable Stimulation of Innate Immunity Bioconjug. Chem. 2018-08-13 [PMID: 30067354] (PLA, Mouse)	PLA	Mouse
Aravanis CV, Kapelouzou A, Vagios S et al. Toll-Like Receptors-2,-3,-4 and-7 Expression Patterns in the Liver of a CLP-Induced Sepsis Mouse Model. J Invest Surg. 2018-05-30 [PMID: 29847187] (IF/IHC)	IF/IHC
Makinen, LK. Matrix metalloproteinases and toll-like receptors in early-stage oral tongue squamous cell carcinoma J Oral Pathol Med 2018-05-11 [PMID: 29747237]
Murayama Goh, Furusawa Nanako, Chiba Asako et al. Enhanced IFN-a production is associated with increased TLR7 retention in the lysosomes of palasmacytoid dendritic cells in systemic lupus erythematosus. Arthritis Research & Therapy 2017-01-01 [PMID: 29052537] (FLOW, Human)	FLOW	Human
Auger Jean-Philippe, Santinon Agustina, Roy David et al. Type I Interferon Induced by Streptococcus suis Serotype 2 is Strain-Dependent and May Be Beneficial for Host Survival. Frontiers in Immunology 2017-08-28 [PMID: 28894449] (BA, Mouse)	BA	Mouse
Jeon YK, Kim CK, Koh J et al. Pellino-1 confers chemoresistance in lung cancer cells by upregulating cIAP2 through Lys63-mediated polyubiquitination. Oncotarget. 2016-07-05 [PMID: 27248820] (Cytometric Bead Assay Standard, Human)	Cytometric Bead Assay Standard	Human
Jouhi L, Mohamed H, Makitie A, Remes SM et al. Toll-like receptor 5 and 7 expression may impact prognosis of HPV-positive oropharyngeal squamous cell carcinoma patients Cancer Immunol. Immunother. 2017-08-30 [PMID: 28856441] (Human)		Human
Feng Y, Zou L, Yan D et al. Extracellular MicroRNAs Induce Potent Innate Immune Responses via TLR7/MyD88-Dependent Mechanisms J. Immunol. 2017-08-02 [PMID: 28768728] (ICC/IF, Rat)	ICC/IF	Rat
To EE, Vlahos R, Luong R et al. Endosomal NOX2 oxidase exacerbates virus pathogenicity and is a target for antiviral therapy Nat Commun 2017-07-12 [PMID: 28701733] (ICC/IF, Mouse)	ICC/IF	Mouse
Larsson O, Tengroth L, Xu Y et al. Substance P represents a novel first-line defense mechanism in the nose. J Allergy Clin Immunol. [PMID: 28219705] (FLOW, ICC/IF, IF/IHC, Mouse) Details: Citation using the FITC form of this antibody.	FLOW, ICC/IF, IF/IHC	Mouse
Daley D, Zambirinis CP, Seifert L et al. gammadelta T Cells Support Pancreatic Oncogenesis by Restraining alphabeta T Cell Activation. Cell 2016-08-25 [PMID: 27569912] (FLOW, Human, Mouse)	FLOW	Human, Mouse
Li X, Liu F, Zhang X et al. Notch-Hes-1 axis controls TLR7-mediated autophagic death of macrophage via induction of P62 in mice with lupus Cell Death Dis 2016-08-19 [PMID: 27537524] (FLOW, Rat)	FLOW	Rat
Huang AY, Wu SY. The effect of imiquimod on taste bud calcium transients and transmitter secretion. Br. J. Pharmacol. 2016-07-27 [PMID: 27464850] (IHC-P, Human)	IHC-P	Human
Zeng M, Nouri-Shirazi E, Guinet E, Nouri-Shirazi M. The genetic background influences the cellular and humoral immune responses to vaccines Clin. Exp Immunol. [PMID: 27393001] (FLOW, Mouse) Details: Citation using the Azide Free form of this antibody.	FLOW	Mouse
Helley MP, Abate W, Jackson SK et al. The expression of Toll-like receptor 4, 7 and co-receptors in neurochemical sub-populations of rat trigeminal ganglion sensory neurons. Neuroscience. 2015-12-03 [PMID: 26434622] (ICC/IF, WB, Rat)	ICC/IF, WB	Rat
Renkonen J, Toppila-Salmi S, Joenvaara S et al. Expression of Toll-like receptors in nasal epithelium in allergic rhinitis APMIS. 2015-06-08 [PMID: 26061394] (IHC-P, Human) Details: TLR7 antibody (Imgenex IMG-581A) was used for IHC-P on nasal biopsies from healthy non-smoking volunteers both allergic to birch pollen and non-allergic controls. Nasal biopsies were collected before and after off-seasonal intranasal birch pollen or diluent challenge and the immunostaining assay implicated - 4um paraffin sections, antigen retrieval withTris-HCl pH 8.5, processing on Autostainer, use of primary at 1.67 ug/mL concentration, Rabbit/Mouse REAL EnVision Detection system and Peroxidase/DAB+ detection (Fig 1, Fig 2D ).	IHC-P	Human
Renkonen S, Cardell LO, Mattila P et al. Toll-like receptors 3, 7, and 9 in Juvenile nasopharyngeal angiofibroma APMIS 2015-05-01 [PMID: 25912131] (IHC-P, Human) Details: TLR7 antibody was used for IHC-P application on formalin-fixed and paraffin-embedded sections from human juvenile nasopharyngeal angiofibroma/JNA patients and the immunoassay implicated : 4um sections, antigen retrieval via Lab Vision PT Module for 20 minutes at 98C, staining on Autostainer 480 with 1 hour primary incubation and REAL EnVision Detection System using REAL DAB+ Chromogen (Figure 2).	IHC-P	Human
Saito K, Kukita K, Kutomi G et al. Heat shock protein 90 associates with Toll-like receptors 7/9 and mediates self-nucleic acid recognition in SLE Eur J Immunol 2015-04-13 [PMID: 25871979] (ICC/IF, Mouse) Details: This citation used the FITC version of this antibody.	ICC/IF	Mouse
Jouhi L, Koljonen V, Bohling T et al. The Expression of Toll-like Receptors 2, 4, 5, 7 and 9 in Merkel Cell Carcinoma Anticancer Res. 2015-04-01 [PMID: 25862837] (IHC-P, Human) Details: TLR7 antibody was used for IHC-P application on tissue microarray/TMA slides from Merkel Cell Carcinoma patients with data on Merkel cell polyomavirus/MCV status and clinical features.	IHC-P	Human
Milara Javier, Morell Anselm, Ballester Bea et al. Roflumilast improves corticosteroid resistance COPD bronchial epithelial cells stimulated with toll like receptor 3 agonist. RespiRatory Research 2015-01-01 [PMID: 25652132] (WB, IF/IHC, Human)	WB, IF/IHC	Human
Makinen LK, Atula T, Hayry V et al. Predictive role of toll-like receptors 2, 4, and 9 in oral tongue squamous cell carcinoma. Oral Oncol. 2014-09-25 [PMID: 25264223] (IHC-P, Human) Details: TLR7 antibody used at 1:300 dilution for IHC-P on oral tongue squamous cell carcinoma tissues / OTSCC (Stage I-II patients) - TMA paraffin sections, antigen retrieval with PreTreatment-module in Tris-HCl buffer pH 8.5 at 98C for 20 min, Autostainer based assay with 1 hour primary incubation time followed by detection using REAL EnVision/HRP-DAB system (Fig 1, Nuclear membranous staining pattern).	IHC-P	Human
Yin Chaoran, Zhang Ting, Qiao Liangjun et al. TLR7-expressing cells comprise an interfollicular epidermal stem cell population in murine epidermis. Sci Rep. 2014-01-01 [PMID: 25060222] (Mouse)		Mouse
Tengroth L, Millrud C, Kvarnhammar A, Kumlien Georen S, Latif L, Cardell L Functional effects of Toll-like receptor (TLR)3, 7, 9, RIG-I and MDA-5 stimulation in nasal epithelial cells. PLoS One 2013-11-30 [PMID: 24886842] (Flow Cytometry Control, Human) Details: Citation using the HRP format of this antibody.	Flow Cytometry Control	Human
Hernandez, C M, Cortez, I Et al. Research tool: Validation of floxed alpha 7 nicotinic acetylcholine receptor conditional knockout mice using in vitro and in vivo approaches. J Physiol 2014-08-01 [PMID: 24879866] (Mouse)		Mouse
Sriram U, Xu J, Chain RW et al. IL-4 Suppresses the Responses to TLR7 and TLR9 Stimulation and Increases the Permissiveness to Retroviral Infection of Murine Conventional Dendritic Cells. PLoS ONE. 2014-02-03 [PMID: 24489947] (ICC/IF, Mouse) Details: IF (mouse bone marrow-derived conventional dendritic cells), Fig 7C.	ICC/IF	Mouse
Takaki H, Takeda M, Tahara M et al. The MyD88 pathway in plasmacytoid and CD4+ dendritic cells primarily triggers type I IFN production against measles virus in a Mouse infection model J Immunol 2013-11-01 [PMID: 24078691] (FLOW, Mouse) Details: This citation used the FITC version of this antibody.	FLOW	Mouse
Abdi J, Mutis T, Garssen J, Redegeld F. Characterization of the Toll-like receptor expression profile in human multiple myeloma cells. PLoS One. 2013-04-08 [PMID: 23593278] Details: Antibodies cited [multiple myeloma (MM) cell lines, human intestine tissue lysate, PBMC, mouse splenocytes] for WB (Figs 2, 3, 4A, 4B, 5, Table 1) and Flow (Intracellular): Figs 4C, 6-8, Table 1: 1. TLR1 (IMG-5012) 2. TLR2 (IMG-416A)3. TLR3 (IMG-315A)4 TL
Caceres NE, Aerts M, Marquez B et al. Analysis of the membrane proteome of ciprofloxacin-resistant macrophages by stable isotope labeling with amino acids in cell culture (SILAC). PLoS One. 2013-01-01 [PMID: 23505477]
Harman AN, Bye CR, Nasr N et al. Identification of lineage relationships and novel markers of blood and skin human dendritic cells. J Immunol. 2013-01-01 [PMID: 23183897] Details: Antibodies cited in Fig 4B for flow cytometric analysis of TLR expression in human monocyte-derived dendritic cells (MDDC), CD14+ monocytes, myeloid DC, and plasmacytoid DC:1. TLR2-FITC, clone T2.1 (IMG-416C): Flow (cell surface)2. TLR4-PE, clone HTA124 (
Ochi A, Graffeo CS, Zambirinis CP et al. Toll-like receptor 7 regulates pancreatic carcinogenesis in mice and humans. J Clin Invest. 2012-11-01 [PMID: 23023703]
Proskocil B, Wai K, Lebold K, et al. TLR7 Is Expressed by Support Cells, but Not Sensory Neurons, in Ganglia Research Square 2021-01-05 [PMID: 22565312] (IF/IHC, Mouse)	IF/IHC	Mouse
Casado Enrique, Garcia Victor Moreno, Sanchez Jose Javier et al. A combined strategy of SAGE and quantitative PCR Provides a 13-gene signature that predicts preoperative chemoradiotherapy response and outcome in rectal cancer. Clin Cancer Res. 2011-06-15 [PMID: 21467161] (Human)		Human
Liu T, Xu ZZ, Park CK et al. Toll-like receptor 7 mediates pruritus. Nat Neurosci. [PMID: 21037581] Details: Citation using the Azide Free form of this antibody.
Krejbich-Trotot P, Denizot M, Hoarau JJ et al. Chikungunya virus mobilizes the apoptotic machinery to invade host cell defenses. FASEB J. 2011-01-01 [PMID: 20881210] (ICC/IF, Human) Details: TLR7 (IMG-581A). IF: Human macrophages infected with different inoculums of chikungunya virus (CHIkV), Fig 7A.	ICC/IF	Human
Xirakia C, Koltsida O, Stavropoulos A et al. Toll-like receptor 7-triggered immune response in the lung mediates acute and long-lasting suppression of experimental asthma. Am J Respir Crit Care Med. 2010-06-01 [PMID: 20224068] (ICC/IF, Mouse) Details: TLR7 (IMG-581A) IF/ICC, Fig E2, mouse lung epithelial cells.	ICC/IF	Mouse
Clancy RM, Alvarez D, Komissarova E et al. Ro60-associated single-stranded RNA links inflammation with fetal cardiac fibrosis via ligation of TLRs: a novel pathway to autoimmune-associated heart block. J Immunol. 2010-02-15 [PMID: 20089705] (Flow Cytometry Control, Human) Details: Antibodies cited: 1. TLR-7 (IMG-665A): Primary human macrophages derived from PBMCs, Flow (Intracellular): Fig 1A 2. TLR-8 FITC (IMG-321C): Primary human macrophages derived from PBMCs Flow (Intracellular): Fig 1A 3. TLR-7 (IMG-581A): Fetal cardiac fibrob	Flow Cytometry Control	Human
Wu J, Meng Z, Jiang M et al. Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific. Immunology. [PMID: 19922426] Details: Citation using the PE/Cy5 form of this antibody.
Barajon I, Serrao G, Arnaboldi F et al. Toll-like receptors 3, 4, and 7 are expressed in the enteric nervous system and dorsal root ganglia. J Histochem Cytochem. 2009-11-01 [PMID: 19546475] (ICC/IF, Human) Details: TLR7 (IMG-581A). IHC (paraffin): Submucous plexus of murine intestine (Fig 1), murine small bowel (Fig 3), and myenteric plexus from human intestine (Fig 4). IF/ICC: Murine myenteric plexus, Fig 2.	ICC/IF	Human
Vatakis Dimitrios N, Nixon Christopher C, Bristol Gregory, Zack Jerome A. Differentially stimulated CD4+ T cells display altered human immunodeficiency virus infection kinetics: implications for the efficacy of antiviral agents J Virol [PMID: 19129455] Details: Used the FITC form of this antibody.
Schwartz Michael J, Liu Huixian, Hwang David H et al. Antitumor effects of an imidazoquinoline in renal cell carcinoma. Urology. 2009-05-01 [PMID: 19118885]
Pries Ralph, Wulff Sandra, Kesselring Rebecca et al. Up-regulation of NK cell function against head and neck cancer in response to ss-isRNA requires TLR7. Int J Oncol. 2008-11-01 [PMID: 18949362] (Human)		Human
Clark RA, Huang SJ, Murphy GF et al. Human squamous cell carcinomas evade the immune response by down-regulation of vascular E-selectin and recruitment of regulatory T cells. J Exp Med. 2008-09-29 [PMID: 18794336] (ICC/IF, Human) Details: Antibodies cited: 1. TLR8 (IMG-321A): IF/ICC (human squamous cell carcinoma tumor cells), Fig. 3A. 2. TLR7 (IMG-581A): IF/ICC (human squamous cell carcimona tumor cells), Fig. 3A.	ICC/IF	Human
Kalali BN, Kollisch G, Mages J et al. Double-stranded RNA induces an antiviral defense status in epidermal keratinocytes through TLR3-, PKR-, and MDA5/RIG-I-mediated differential signaling. J Immunol. 2008-08-15 [PMID: 18684960] (IHC-Fr, Human) Details: IHC (frozen cryostat sections): human skin biopsies, Fig 6A, B. Stronger TLR7 staining was seen in human papillomavirus-infected skin compared to healthy skin.	IHC-Fr	Human
Palazzo Marco, Gariboldi Silvia, Zanobbio Laura et al. Cross-talk among Toll-like receptors and their ligands. Int Immunol. 2008-05-01 [PMID: 18397908]
Smith EB, Schwartz M, Kawamoto H et al. Antitumor effects of imidazoquinolines in urothelial cell carcinoma of the bladder. J Urol. 2007-06-01 [PMID: 17509356] (WB) Details: Antibodies cited: 1. TLR7 (IMG-581) [WB, Fig.4 (MBT-2, TCCSUP, T24, and J82 cancer cell lines)].	WB
Brinkmann MM, Spooner E, Hoebe K et al. The interaction between the ER membrane protein UNC93B and TLR3, 7, and 9 is crucial for TLR signaling. J Cell Biol. 2007-04-23 [PMID: 17452530] (IP, Mouse) Details: IMG-581A (TLR7): IP (mouse bone marrow-derived dendritic cells), Fig. 6A,B,C.	IP	Mouse
Palladino M A, Johnson T A, Gupta R et al. Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tract. Biol Reprod. 2007-06-01 [PMID: 17314314]
Patole PS, Pawar RD, Lech M et al. Expression and regulation of Toll-like receptors in lupus-like immune complex glomerulonephritis of MRL-Fas(lpr) mice. Nephrol Dial Transplant. 2006-11-01 [PMID: 16954173] (IHC-Fr, Mouse) Details: For IHC (frozen) on mouse kidney sections (Fig. 5): TLR3 (IMG-516), TLR7 (IMG-581A), TLR9 (IMG-431) and Rabbit IgG isotype control antibody (20304). For Flow (Intacellular) and Flow (Cell surface) on J774 mouse macrophage-like cell line (Fig 6B): TLR7 (IMG-581A), TLR9 (IMG-431) and Rabbit IG isotype control antibody (20304).	IHC-Fr	Mouse
Lanki M Prognostic and Differential Diagnostic Biomarkers in Pancreatic Ductal Adenocarcinoma Thesis 2023-01-01 (IHC-P, Human)	IHC-P	Human
Pawar RD, Patole PS, Zecher D et al. Toll-like receptor-7 modulates immune complex glomerulonephritis. J Am Soc Nephrol. 2006-01-01 [PMID: 16280469] Details: Antibodies cited: 1. TLR3 (IMG-516) 2. TLR7 (IMG-581) [Flow (intracellular and cell surface), Fig.3: TLR3 (mesengial cells and J774 macrophages), TLR7 (J774 macrophages)]. [IHC-F, Fig.2 (kidneys of MRL1pr/1pr mice)].
Hart OM, Athie-Morales V, O'Connor GM, Gardiner CM. TLR7/8-mediated activation of human NK cells results in accessory cell-dependent IFN-gamma production. J Immunol. 2005-08-01 [PMID: 16034103] (WB, Human) Details: Antibodies cited: 1. TLR7 [(IMG-581A) WB, Fig. 1: TLR7 transfected cells and NKL human leukemia cell line. The specificity of the TLR7 antibody has been validated by WB using overexpressed TLR7 in 293T cells in Fig. 1.]. 2. TLR8 [(IMG-321A) WB, Fig. 1: TL	WB	Human
Srivastava MD, Srivastava BI. Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line. Leuk Res. 2005-07-01 [PMID: 15927677] (ICC/IF) Details: IF/ICC in SRIK-NKL cells: TLR2 (IMG416), TLR4 (IMG-417), TLR7 (IMG-581), and TLR9 (IMG-305).	ICC/IF
Francois S et al. Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad J Immunol [PMID: 15749901] (WB, Human) Details: Used the FITC form of this antibody.	WB	Human
Show All 80 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	IHC-Fr	Mouse	08/02/2019	View Summary Application Immunohistochemistry-Frozen Sample Tested Fixe mouse brain tissue Species Mouse Lot 0221872A-3
Enlarge	5 reviewed by: Verified Customer	WB	Rat	12/02/2017	View Summary Application Western Blot Sample Tested Dorsal root ganglion neurons Species Rat Lot 043015
Enlarge	5 reviewed by: Verified Customer	IHC-Fr	Mouse	08/29/2015	View Summary Application Immunohistochemistry-Frozen Sample Tested Mouse Ear Skin (frozen section) Species Mouse
Enlarge	5 reviewed by: Verified Customer	IF	Mouse	08/28/2015	View Summary Application Immunofluorescence Sample Tested Mouse Ear Skin (frozen section) Species Mouse

Array NBP2-24906

• TLR7 Antibodies
• TLR7 Antibody Pack
• TLR7 ELISA Kit
• TLR7 Proteins
• TLR7 Proteins and Enzymes