NOD2 Antibody (2D9) - BSA Free

The NOD2 (nucleotide-binding oligomerization domain containing 2) protein is expressed in the antigen-presenting cells (monocytes and macrophages) and in intestinal Paneth cells, keratinocytes, T cells, NK cells, and CD34+ stem cells. Alongwith Apaf-1 and CIITA, NOD2 belongs to NLR/NOD leucine-rich repeat containing protein family. NOD2 generally localizes to the cytoplasm from where it translocates to cell membranes, a mechanism suggested as important for its functionality. After interacting with CARD-CARD, NOD2 may bind/activate RIP2/RICK and this active NOD2:RIP2 complex then stimulates MAPK (JNK, ERK, p38), NF-kB, autophagy and antigen presentation. NOD2 has an ability to senses the presence of MDP (muramyl dipeptide), a breakdown product of peptidoglycan found in bacteria, and after recognition of intracellular bacteria, NOD2 pathway execute directing the secretion of antimicrobial peptides and cytokines/chemokines to induce innate immune clearance of the pathogen as well as to tailor the adaptive immune system to help fight the bacterial infection. Defects in NOD2 are the cause of Blau syndrome (BS), susceptibility to inflammatory bowel disease type 1 (IBD1) and sarcoidosis early-onset (EOS).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: FLOW, ICC/IF, IF/IHC, IHC-P, IP, WB.

Showing Publications 1 - 10 of 26. Show All 26 Publications. Collapse Publications.

Publications using NB100-524	Applications	Species
Thakker P, Ariana A, Hajjar S et al. XIAP promotes the expansion and limits the contraction of CD8 T cell response through cell extrinsic and intrinsic mechanisms respectively PLoS pathogens 2023-06-22 [PMID: 37347786] (WB, Mouse)	WB	Mouse
Li X, Liu S, Jin L et al. NOD2 inhibits the proliferation of esophageal adenocarcinoma cells through autophagy Journal of cancer research and clinical oncology 2022-10-31 [PMID: 36316517] (WB, Human)	WB	Human
Hardman CS, Chen YL, Salimi M et al. IL-6 effector function of group 2 innate lymphoid cells (ILC2) is NOD2 dependent Science Immunology 2021-05-28 [PMID: 34021026]
Huaman M, Qualls J, Jose S et al. Mycobacterium bovis Bacille-Calmette-GuErin Infection Aggravates Atherosclerosis Frontiers in Immunology 2020-12-18 [PMID: 33391278] (FLOW, Mouse)	FLOW	Mouse
Mai E, Percopo CM, Limkar AR et al Respiratory Epithelial Cells Respond to Lactobacillus plantarum but Provide No Cross-Protection against Virus-Induced Inflammation Viruses 2020-12-30 [PMID: 33374950] (FLOW, Mouse) Details: Citation using the Azide Free version of this antibody.	FLOW	Mouse
Lipinski S, Petersen BS, Barann M et al. Missense variants in NOX1 and p22phox in a case of very-early-onset inflammatory bowel disease are functionally linked to NOD2 Cold Spring Harb Mol Case Stud. 2017-11-07 [PMID: 30709874] (WB, IP, Human)	WB, IP	Human
Singh K, Han K, Tilve S et al. Parkin targets NOD2 to regulate astrocyte endoplasmic reticulum stress and inflammation. Glia. 2018-11-01 [PMID: 30378174] (IP, Mouse)	IP	Mouse
Li J, Li B, Cheng Y et al. The synergistic effect of NOD2 and TLR4 on the activation of autophagy in human submandibular gland inflammation. J. Oral Pathol. Med. 2018-10-26 [PMID: 30367515] (WB, Human)	WB	Human
Subramanian K1, Bergman P, Henriques-Normark B. Vitamin D Promotes Pneumococcal Killing and Modulates Inflammatory Responses in Primary Human Neutrophils. J Innate Immun. [PMID: 28241127] (FLOW, Human) Details: Citation using the FITC form of this antibody.	FLOW	Human
Kapoor A, Forman M, Arav-Boger R. Activation of Nucleotide Oligomerization Domain 2 (NOD2) by Human Cytomegalovirus Initiates Innate Immune Responses and Restricts Virus Replication. PLoS ONE 2014-03-27 [PMID: 24671169] (WB, Human)	WB	Human
Marischen L, Wesch D, Oberg HH et al. Functional expression of NOD2 in freshly isolated human peripheral blood ?d T-cells. Scand J Immunol. 2011-03-15 [PMID: 21410503]
Tikka C, Manthari R, Niu R, et al. Dendritic cell CX3CR1 and macrophages F4/80 play a central role in between gut micro biome and inflammation in Arsenic induced mice bioRxiv 2021-01-26 (IF/IHC, Mouse)	IF/IHC	Mouse
Zhang L, Zhang B, Wei M et Al. TRIM22 inhibits endometrial cancer progression through the NOD2/NF kappa B signaling pathway and confers a favorable prognosis Int J Oncol 2020-03-04 [PMID: 32319602] (ICC/IF, WB, IF/IHC, IHC-P, Human)	ICC/IF, WB, IF/IHC, IHC-P	Human
Tikka C, Manthari R, Ommati M, et al. Immune disruption occurs through altered gut microbiome and NOD2 in arsenic induced mice: Correlation with colon cancer markers Chemosphere 2020-01-01 [PMID: 31927375] (ICC/IF, IF/IHC, Mouse)	ICC/IF, IF/IHC	Mouse
Valdez-Miramontes CE, Trejo MartInez LA, Torres-JuArez F et al. Nicotine modulates molecules of the innate immune response in epithelial cells and macrophages during infection with M. tuberculosis Clin Exp Immunol. 2019-11-03 [PMID: 31631328] Details: Citation used the Alexa Fluor 647 format of this antibody.
Mey L, Jung M, Roos F et al. NOD1 and NOD2 of the innate immune system is differently expressed in human clear cell renal cell carcinoma, corresponding healthy renal tissue, its vasculature and primary isolated renal tubular epithelial cells J. Cancer Res. Clin. Oncol. 2019-03-22 [PMID: 30903318] (WB, FLOW, Human)	WB, FLOW	Human
Lipinski S, Grabe N, Jacobs et al. RNAi screening identifies mediators of NOD2 signaling: Implications for spatial specificity of MDP recognition Proc Natl Acad Sci U S A 2012-12-03 [PMID: 23213202] (IF/IHC, ICC/IF, WB, Human)	IF/IHC, ICC/IF, WB	Human
Billmann-Born S, Till A, Arlt A et al. Genome-wide expression profiling identifies an impairment of negative feedback signals in the Crohn's disease-associated NOD2 variant L1007fsinsC J Immunol 2011-04-01 [PMID: 21335489] (WB, Human)	WB	Human
von Kampen O, Lipinski S, Till A, Martin SJ, Nietfeld W, Lehrach H, Schreiber S, Rosenstiel P. Caspase recruitment domain-containing protein 8 (CARD8) negatively regulates NOD2-mediated signaling. J Biol Chem;285(26):19921-6. 2010-06-25 [PMID: 20385562] (ICC/IF, Human)	ICC/IF	Human
Cooney R et al. NOD2 stimulation induces autophagy in dendritic cells influencing bacterial handling and antigen presentation. Nat. Med. Dec 6 2009. 2009-01-01 [PMID: 19966812]
Lipinski S, Till A, Sina C et al. DUOX2-derived reactive oxygen species are effectors of NOD2-mediated antibacterial responses. J Cell Sci;122(19):3522-3530. 2009-01-01 [PMID: 19759286] (WB, IP, Human)	WB, IP	Human
Chauhan VS, Sterka DG Jr, Furr SR, Young AB, Marriott I. NOD2 plays an important role in the inflammatory responses of microglia and astrocytes to bacterial CNS pathogens. Glia;57(4):414-23. 2009-03-01 [PMID: 18803303] (WB, Mouse)	WB	Mouse
Kim TH, Payne U, Zhang X, Iwanaga Y, Davey MP, Rosenbaum JT, Inman RD. Altered host:pathogen interactions conferred by the Blau syndrome mutation of NOD2. Rheumatol Int;27(3):257-62. 2007-01-01 [PMID: 17096091]
McDonald C, Chen FF, Ollendorff V, Ogura Y, Marchetto S, Lecine P, Borg JP, Nunez G. A role for Erbin in the regulation of Nod2-dependent NF-kappaB signaling. J Biol Chem;280(48):40301-9. 2005-12-02 [PMID: 16203728]
Ogura Y, Lala S, Xin W et al. Expression of NOD2 in Paneth cells: a possible link to Crohn's ileitis. Gut;52(11):1591-7. 2003-11-01 [PMID: 14570728] (IHC-P, Mouse)	IHC-P	Mouse
Lala S, Ogura Y, Osborne C et al. Crohn's disease and the NOD2 gene: a role for paneth cells. Gastroenterology;125(1):47-57. 2003-07-01 [PMID: 12851870] (IF/IHC, Human)	IF/IHC	Human
Show All 26 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Alexander Chamessian	IF	Mouse	10/03/2014	View Summary Application Immunofluorescence Sample Tested PFA fixed Mouse Sciatic Nerve Slice Species Mouse

Array NB100-524

• NOD2 Antibodies
• NOD2 Lysate
• NOD2 Proteins