B7-2/CD86 Antibody (BU63)

B7-2, also referred to as CD86, is a glycosylated type 1 membrane protein that is a member of the immunoglobulin (Ig) superfamily (1). B7-2/CD86 is constitutively expressed on antigen presenting cells (APCs) such as dendritic cells (DCs), macrophages, and B cells and functions in controlling immune responses through either costimulatory or coinhibitory signals (1,2). Expression of B7-2 is upregulated by APCs upon activation and can be induced in T cells (1,3). The human B7-2 protein, encoded by the CD86 gene, is 329 amino acids (aa) in length with a theoretical molecular weight (MW) of 37.6 kDa (4). The B7-2 protein contains characteristic Ig variable-like (IgV) and constant-like (IgC) domains within its extracellular region (1,3). B7-2/CD86 has structural similarity and shares ~25% sequence homology with another B7 family molecule, B7-1/CD80 (3,5). Both B7-1 and B7-2 are ligands for the activating receptor CD28 and the regulatory receptor cytotoxic T-lymphocyte antigen 4 (CTLA-4) which are expressed on subsets of T cells (1-3,5-7). However, B7-1 and B7-2 bind to CTLA-4 with higher affinity than CD28 (3,5,6). B7-2/CD86 binding to CD28 results in costimulatory signals to promote T cell activation, proliferation, and cytokine production (1-3. 5-7). Binding to CTLA-4 initiates coinhibitory signaling to attenuate the pro-inflammatory T cell response, while also promoting the suppressive function of regulatory T (Treg) cells through expression of indoleamine 2,3-deoxygenase (IDO) (1-3,6,7). Molecules involved in T cell co-signaling have become considerable targets of interest for cancer immunotherapy (7). The anti-CTLA-4 monoclonal antibody ipilimumab, first approved for the treatment of metastatic melanoma, prevents B7-1/B7-2 molecules from binding to CTLA-4, thus driving B7-CD28 binding and promoting costimulatory signals and antitumor effects (1,7,8). Combination therapies targeting co-signaling molecules are currently under investigation to improve antitumor response for treatment of both melanoma and non-melanoma cancers (7,8).

References

1. Collins M, Ling V, Carreno BM. The B7 family of immune-regulatory ligands. Genome Biol. 2005;6(6):223. https://doi.org/10.1186/gb-2005-6-6-223

2. Greaves P, Gribben JG. The role of B7 family molecules in hematologic malignancy. Blood. 2013;121(5):734-744. https://doi.org/10.1182/blood-2012-10-385591

3. Bolandi N, Derakhshani A, Hemmat N, et al. The Positive and Negative Immunoregulatory Role of B7 Family: Promising Novel Targets in Gastric Cancer Treatment. Int J Mol Sci. 2021;22(19):10719. https://doi.org/10.3390/ijms221910719

4. Uniprot (P42081)

5. Bhatia S, Edidin M, Almo SC, Nathenson SG. B7-1 and B7-2: similar costimulatory ligands with different biochemical, oligomeric and signaling properties. Immunol Lett. 2006;104(1-2):70-75. https://doi.org/10.1016/j.imlet.2005.11.019

6. Ohue Y, Nishikawa H. Regulatory T (Treg) cells in cancer: Can Treg cells be a new therapeutic target?. Cancer Sci. 2019;110(7):2080-2089. https://doi.org/10.1111/cas.14069

7. Chen L, Flies DB. Molecular mechanisms of T cell co-stimulation and co-inhibition [published correction appears in Nat Rev Immunol. 2013 Jul;13(7):542]. Nat Rev Immunol. 2013;13(4):227-242. https://doi.org/1010.1038/nri3405

8. Karimi A, Alilou S, Mirzaei HR. Adverse Events Following Administration of Anti-CTLA4 Antibody Ipilimumab. Front Oncol. 2021;11:624780. https://doi.org/101010.3389/fonc.2021.624780

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: ICC/IF, IF, IF/IHC, IHC-Fr, IHC-P, Immunocytochemistry/ Immunofluorescence, WB.

Showing Publications 1 - 10 of 27. Show All 27 Publications. Collapse Publications.

Publications using NBP2-25208	Applications	Species
Luo Y, Ali T, Liu Z et al. EPO prevents neuroinflammation and relieves depression via JAK/STAT signaling Life Sciences 2023-09-01 [PMID: 37769806] (WB, Mouse)	WB	Mouse
Jia H, Wei P, Zhou S et al. Attenuated Salmonella carrying siRNA-PD-L1 and radiation combinatorial therapy induces tumor regression on HCC through T cell-mediated immuno-enhancement Cell Death Discovery 2023-08-28 [PMID: 37640735] (Immunocytochemistry/ Immunofluorescence)	Immunocytochemistry/ Immunofluorescence
Tan Y, Coyle RC, Barrs RW et al. Nanowired human cardiac organoid transplantation enables highly efficient and effective recovery of infarcted hearts Science Advances 2023-08-04 [PMID: 37540743]
Chen S, Xing Z, Geng M et al. Macrophage fusion event as one prerequisite for inorganic nanoparticle-induced antitumor response Science advances 2023-07-21 [PMID: 37467339] (IHC-P, Mouse)	IHC-P	Mouse
Wang B, Chen Y, Chang H et al. Zinc Oxide Nanoparticles Exacerbate Skin Epithelial Cell Damage by Activating M1 Macrophages by Upregulating the Nlrp3 Inflammasome and Exosome Secretion after Uvb Irradiation-Induced Skin Injury SSRN Electronic Journal 2023-02-22 (IHC-P, Mouse)	IHC-P	Mouse
Shi C, Zhang Y, Wu G et al. Hyaluronic Acid-Based Reactive Oxygen Species-Responsive Multifunctional Injectable Hydrogel Platform Accelerating Diabetic Wound Healing Advanced healthcare materials 2023-11-09 [PMID: 37943252]
Yao S, Zhou Z, Wang L et al. Targeting endometrial inflammation in intrauterine adhesion ameliorates endometrial fibrosis by priming MSCs to secrete C1INH iScience 2023-06-01 [PMID: 37456855]
Chen L, Song X, Yao Z et al. Gelatin nanofiber-reinforced decellularized amniotic membrane promotes axon regeneration and functional recovery in the surgical treatment of peripheral nerve injury Biomaterials 2023-09-01 [PMID: 37352606] (IHC-P)	IHC-P
Tan X, Fang P, Li K et al. A HER2-targeted antibody-novel DNA topoisomerase I inhibitor conjugate induces durable adaptive antitumor immunity by activating dendritic cells mAbs 2023-06-14 [PMID: 37314961] (IHC-P, Human) Details: 1:200 dilution	IHC-P	Human
Ling Q, Fang J, Zhai C et al. Berberine induces SOCS1 pathway to reprogram the M1 polarization of macrophages via miR-155-5p in colitis-associated colorectal cancer European journal of pharmacology 2023-04-13 [PMID: 37059377]
Jia X, Xie L, Liu y et al. Astragalus polysaccharide (APS) exerts protective effect against acute ischemic stroke (AIS) through enhancing M2 micoglia polarization by regulating adenosine triphosphate (ATP)/ purinergic receptor (P2X7R) axis Bioengineered 2022-02-01 [PMID: 35166175] (IHC-P, Rat)	IHC-P	Rat
Luo ZW, Xia K, Liu YW et al. Extracellular Vesicles from Akkermansia muciniphila Elicit Antitumor Immunity Against Prostate Cancer via Modulation of CD8+ T Cells and Macrophages International journal of nanomedicine 2021-04-20 [PMID: 33907401] (IF/IHC, Human)	IF/IHC	Human
Liu P, Peng C, Chen X et al. Acitretin Promotes the Differentiation of Myeloid-Derived Suppressor Cells in the Treatment of Psoriasis Frontiers in Medicine 2021-03-23 [PMID: 33834031] (IHC-P, Mouse)	IHC-P	Mouse
Yang D, Shen L, Chen R et al. The Effect of Talaromyces Marneffei Infection on CD86 Expression in THP-1 Cells Infect Drug Resist 2021-03-01 [PMID: 33642869]
Klinge U, Dievernich A, Tolba R et al. CD68+ macrophages as crucial components of the foreign body reaction demonstrate an unconventional pattern of functional markers quantified by analysis with double fluorescence staining J. Biomed. Mater. Res. Part B Appl. Biomater. 2020-05-30 [PMID: 32475069] (ICC/IF, Human)	ICC/IF	Human
Li Ml, Su Xm, Ren Y et Al. HDAC8 inhibitor attenuates airway responses to antigen stimulus through synchronously suppressing galectin-3 expression and reducing macrophage-2 polarization Respir. Res. 2020-02-28 [PMID: 32111211] (IF/IHC, Mouse)	IF/IHC	Mouse
Shehata MM, Saad Eldien HM, Meligy FY, Bahaidarh SY The Possible Protective Role of Barley Seeds on the Spleen after Administration of Glucocorticoids in Adult Albino Rats: A Histological and Immunohistochemical Study J Microsc Ultrastruct 2019-11-18 [PMID: 31803571] (Rat)		Rat
Joly-Amado A, Davtyan H, Serraneau K et al. Active immunization with tau epitope in a mouse model of tauopathy induced strong antibody response together with improvement in short memory and pSer396-tau pathology. Neurobiol Dis. 2020-02-01 [PMID: 31629891] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Zhu LF, Li L, Wang XQ et al. M1 macrophages regulate TLR4/AP1 via paracrine to promote alveolar bone destruction in periodontitis Oral Dis 2019-07-30 [PMID: 31361069]
Liu D, Guo M, Zhou P et al. TSLP promote M2 macrophages polarization and cardiac healing after myocardial infarction Biochem. Biophys. Res. Commun. 2019-06-18 [PMID: 31227217] (IF, Mouse)	IF	Mouse
Kaushik NK, Kaushik N, Adhikari M et al. Preventing the Solid Cancer Progression via Release of Anticancer-Cytokines in Co-Culture with Cold Plasma-Stimulated Macrophages Cancers (Basel) 2019-06-18 [PMID: 31216715] (IHC-P, Mouse)	IHC-P	Mouse
Zhao J, Zhang Z, Xue Y et al. Anti-tumor macrophages activated by ferumoxytol combined or surface-functionalized with the TLR3 agonist poly (I : C) promote melanoma regression Theranostics 2018-11-29 [PMID: 30613299] (ICC/IF, Human)	ICC/IF	Human
Ginsenoside Rg3 Mitigates Atherosclerosis Progression in Diabetic apoE-/- Mice by Skewing Macrophages to the M2 Phenotype. Ginsenoside Rg3 Mitigates Atherosclerosis Progression in Diabetic apoE-/- Mice by Skewing Macrophages to the M2 Phenotype. Front Pharmacol. 2018-05-09 [PMID: 29867472] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Li K, Xu W, Chen Y et al. Piezoelectric Nanostructured Surface for Ultrasound?Driven Immunoregulation to Rescue Titanium Implant Infection Advanced Functional Materials 2023-04-18
Rivera Del Alamo M, Diaz-Lobo M, Busquets S et al. Specific expression pattern of tissue cytokines analyzed through the Surface Acoustic Wave technique is associated with age-related spontaneous benign prostatic hyperplasia in rats. Biochem Biophys Rep 2018-01-01 [PMID: 29872731] (WB, Rat)	WB	Rat
W B, Chen Y, Chang H et al. Zinc oxide nanoparticles exacerbate skin epithelial cell damage by upregulating the NLRP3 inflammasome and exosome secretion in M1 macrophages after UVB irradiation-induced skin injury Research Square 2023-09-15 (IHC-P, Mouse)	IHC-P	Mouse
Schliefsteiner C, Peinhaupt M, Kopp S et al. Human Placental Hofbauer Cells Maintain an Anti-inflammatory M2 Phenotype despite the Presence of Gestational Diabetes Mellitus Front Immunol 2017-08-21 [PMID: 28824621] (WB, Human)	WB	Human
Show All 27 Publications. Collapse Publications.

Array NBP2-25208

• B7-2/CD86 Antibodies
• B7-2/CD86 Antibody Pairs
• B7-2/CD86 ELISA Kits
• B7-2/CD86 Proteins
• B7-2/CD86 Proteins and Enzymes
• B7-2/CD86 ELISA