TLR6 Antibody (86B1153.2) - BSA Free

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. The amino acid sequence of human TLR6 is most similar to hTLR1 with 69% identity at the amino acid level. Human TLR6 consists of a predicted 807 amino acids with a molecular weight of approximately 92 kDa. Human and mouse TLR6 share an amino acid identity of 73%. TLR6 activates both NF-kB and c-Jun N-terminal Kinase (JNK). The amino acids in the cytoplasmic domain of IL-1 receptor, which are critical for NF-kB activation, are conserved in TLR6. It has also been shown that TLR2-mediated response to a phenol-soluble factor from staphylococcus epidermidis is enhanced by TLR6. TLR6 is predominantly expressed in spleen, thymus, ovary, and lung.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 9 applications: Block/Neutralize, FLOW, Flow Cytometry, Flow Cytometry Control, Flow-CS, IF/IHC, IHC-P, Immunohistochemistry, WB.

Showing Publications 1 - 10 of 19. Show All 19 Publications. Collapse Publications.

Publications using NB100-56536	Applications	Species
Sato Y, Wakita A, Maeda E et al. High TLR6 Expression Status Predicts a More Favorable Prognosis after Esophagectomy for Locally Advanced Thoracic Esophageal Squamous Cell Carcinoma Current oncology (Toronto, Ont.) 2023-05-04 [PMID: 37232814] (Immunohistochemistry, Human)	Immunohistochemistry	Human
Qiu C, Wang J, Zhu L et al. Improving the ex vivo expansion of human tumor-reactive CD8 + T cells by targeting toll-like receptors Frontiers in Bioengineering and Biotechnology 2022-10-31 [PMID: 36394017] (Flow Cytometry, Block/Neutralize)	Flow Cytometry, Block/Neutralize
Schoniger S, Grafe H, Wipplinger M, Schoon H. Expression of Toll-like receptors 2, 4 and 6 in the equine chorioallantois. BioMed Research International. 2018-11-25 [PMID: 30502912] (IF/IHC)	IF/IHC
Schoniger S, Grafe H, Schoon H. Expression of Toll-like receptors 2, 4 and 6 in different cell populations of the equine endometrium. Veterinary Immunology and Immunopathology. 2017-01-22 [PMID: 28242004]
Renkonen J, Toppila-Salmi S, Joenvaara S et al. Expression of Toll-like receptors in nasal epithelium in allergic rhinitis APMIS. 2015-06-08 [PMID: 26061394] (IHC-P, Human) Details: TLR7 antibody (Imgenex IMG-581A) was used for IHC-P on nasal biopsies from healthy non-smoking volunteers both allergic to birch pollen and non-allergic controls. Nasal biopsies were collected before and after off-seasonal intranasal birch pollen or diluent challenge and the immunostaining assay implicated - 4um paraffin sections, antigen retrieval withTris-HCl pH 8.5, processing on Autostainer, use of primary at 1.67 ug/mL concentration, Rabbit/Mouse REAL EnVision Detection system and Peroxidase/DAB+ detection (Fig 1, Fig 2D ).	IHC-P	Human
Lopez MC, Palmer BE, Lawrence DA. Naive T cells, unconventional NK and NKT cells, and highly responsive monocyte-derived macrophages characterize human cord blood Immunobiology 2014-06-11 [PMID: 24986635] (Flow-CS, Human) Details: This citation used the PE version of this antibody.	Flow-CS	Human
Regan T, Nally K, Carmody R et al. Identification of TLR10 as a Key Mediator of the Inflammatory Response to Listeria monocytogenes in Intestinal Epithelial Cells and Macrophages. J Immunol 2013-12-15 [PMID: 24198280] (FLOW)	FLOW
Harman AN, Bye CR, Nasr N et al. Identification of lineage relationships and novel markers of blood and skin human dendritic cells. J Immunol. 2013-01-01 [PMID: 23183897] Details: Antibodies cited in Fig 4B for flow cytometric analysis of TLR expression in human monocyte-derived dendritic cells (MDDC), CD14+ monocytes, myeloid DC, and plasmacytoid DC:1. TLR2-FITC, clone T2.1 (IMG-416C): Flow (cell surface)2. TLR4-PE, clone HTA124 (
Oberg HH, Ly TT, Ussat S et al. Differential but direct abolishment of human regulatory T cell suppressive capacity by various TLR2 ligands. J Immunol. 2010-05-01 [PMID: 20363971] (Flow Cytometry Control) Details: Flow(intracellular) cytometry: TLR2/CD282 PE (IMG-416D) and TLR6/CD286 (IMG-304A). T cells and Tregs were separated from freshly isolated human PBMCs, Fig S3.	Flow Cytometry Control
van den Berk LC, Jansen BJ, Siebers-Vermeulen KG et al. Toll-like receptor triggering in cord blood mesenchymal stem cells. J Cell Mol Med. 2009-09-01 [PMID: 20196781] (Flow-CS, Human) Details: flow (cell surface) cytometry: TLR5 (IMG-663A), TLR6 (IMG-304), TLR8 (IMG-321). Human mesenchymal stem cells, Fig 1C.	Flow-CS	Human
Pietschmann K, Beetz S, Welte S et al. Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes. Scand J Immunol. 2009-09-01 [PMID: 19703014] (Flow Cytometry Control, Human)	Flow Cytometry Control	Human
Pegu A, Qin S, Fallert Junecko BA et al. Human lymphatic endothelial cells express multiple functional TLRs. J Immunol. 2008-03-01 [PMID: 18292566] Details: Antibodies cited [Flow (Cell surface) and Flow (Intracellular), human lymphatic endothelial cells, Fig. 1D]: 1. IMG-663C (TLR5-FITC) 2. IMG-304C (TLR6-FITC).
Peiser M, Koeck J, Kirschning CJ et al. Human Langerhans cells selectively activated via Toll-like receptor 2 agonists acquire migratory and CD4+T cell stimulatory capacity. J Leukoc Biol. 2008-05-01 [PMID: 18252867] (Flow-CS) Details: IMG-304A Flow (Cell Surface), Fig. 1C [primary human Langerhans cells (LC), LC cells generated from monocytes, and dendritic cells generated from monocytes].	Flow-CS
Yavuz S, Elbir Y, Tulunay A et al. Differential expression of toll-like receptor 6 on granulocytes and monocytes implicates the role of microorganisms in Behcet's disease etiopathogenesis. Rheumatol Int. 2008-03-01 [PMID: 17934735] (Flow Cytometry Control, Human) Details: Flow (intracellular), human peripheral blood mononuclear cells and granulocytes, Figs. 1, 3.	Flow Cytometry Control	Human
Murciano C, Villamon E, Yanez A et al. In vitro response to Candida albicans in cultures of whole human blood from young and aged donors. FEMS Immunol Med Microbiol. 2007-11-01 [PMID: 17714490] Details: The following anitbodies were used in flow (cell surface) cytometry: TLR2/CD282 FITC (IMG-416C), TLR4 FITC (IMG-417C), TLR6/CD286 FITC (IMG-304C). Human whole blood from both young and elderly volunteers, Fig 1. Note: The fluorescence mean intensity was m
Wong CK, Cheung PF, Ip WK et al. Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils. Am J Respir Cell Mol Biol. 2007-07-01 [PMID: 17332440] (WB, Human) Details: Human blood eosinophils and neutrophils from buffy coat: For WB, Fig. 1A: TLR1 (IMG-5012), TLR5 (IMG-664), TLR6 (IMG-304A), TLR7 (IMG-540), TLR8 (IMG-321A), TLR9 (IMG-305A). For Flow (Intracellular) and Flow (Surface), Fig. 1B: TLR1 (IMG-5021), TLR2 (IMG-416C), TLR3 (IMG-315C), TLR4 (IMG-417C), TLR5 (IMG-663C), TLR6 (IMG-304C), TLR7 (IMG-665A), TLR8 (IMG-321C), TLR9 (IMG-305C).	WB	Human
Hajishengallis G, Tapping RI, Harokopakis E et al. Differential interactions of fimbriae and lipopolysaccharide from Porphyromonas gingivalis with the Toll-like receptor 2-centred pattern recognition apparatus. Cell Microbiol. 2006-10-01 [PMID: 16984411]
Ganley-Leal LM, Liu X, Wetzler LM. Toll-like receptor 2-mediated human B cell differentiation Clin Immunol 2006-09-01 [PMID: 16766226] (FLOW) Details: This citation used the Biotin version of this antibody.	FLOW
Cognasse F, Hamzeh H, Chavarin P et al. Evidence of Toll-like receptor molecules on human platelets. Immunol Cell Biol. 2005-04-01 [PMID: 15748217] Details: TLR2-PE (IMG-416D), TLR4-PE (IMG-417D), TLR6 (IMG-304A), TLR8-PE (IMG-321D), TLR9-PE (IMG-305D). Applications: Intracellular Flow Cytomety and Cell Surface Flow Cytometry: Figs 1 and 2. A comparison of staining results, intracellular versus cell surface flow cytometry is shown. Cell type: Human platelets.
Show All 19 Publications. Collapse Publications.

Array NB100-56536

• TLR6 Antibodies
• TLR6 ELISA Kit
• TLR6 Lysate
• TLR6 Proteins
• TLR6 Proteins and Enzymes