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Goat anti-Mouse IgG Secondary Antibody [HRP]

Images

 
Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was ...read more
Detection of Myocilin in human skeletal muscle using 5 ug/mL of Mouse monoclonal anti-human Myocilin primary antibody (MAB3446) followed by 30 min incubation with Goat anti Mouse HRP conjugated secondary antibodies ...read more
Inverse correlation between the expression of MITF-M and that of miR-155 in melanoma cell lines incubated with IL-1 beta .(A) The expression of MITF-M, Tyrosinase and miR-155 was analyzed by quantitative RT-PCR in 8 ...read more
miR-155 downregulates MITF-M in LB2201-MEL melanoma cell line.(A) MITF-M and (B) Tyrosinase expression was analyzed by quantitative RT-PCR in melanoma cell line LB2201-MEL 24h and 48h after the transfection with a mimic ...read more
Quantitative and qualitative analysis of protein synthesis in wild-type and Mstn−/− Tibialis anterior and gastrocnemius muscle using SUnSET.(A-B) Slot-blot western analysis for quantification of protein synthesis in ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
HRP

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Goat anti-Mouse IgG Secondary Antibody [HRP] Summary

Immunogen
Mouse IgG
Specificity
Detects mouse IgG1, IgG2A, IgG2B, and IgG3 heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with human IgG and rabbit IgG is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Western Blot
Reviewed Applications
Read 3 Reviews rated 4.7
using
HAF007 in the following applications:

Publications
Read Publications using
HAF007 in the following applications:

Packaging, Storage & Formulations

Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Sodium Phosphate and Proclin 300.
Preservative
Proclin 300

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat anti-Mouse IgG Secondary Antibody [HRP]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (HAF007)(248)

We have publications tested in 17 confirmed species: Human, Mouse, Rat, Bat, Canine, E. coli, Macaca mulatta (Rhesus Macaque), Mycobacterium smegmatis, Ovine, Plasmodium falciparum, Porcine, Primate - C. aethiops, Primate - Macaca fascicularis (Cynomolgus Macaque), Transgenic Mouse, Xenograft, Yeast, Yeast - Saccharomyces cerevisiae.

We have publications tested in 16 applications: Bioassay, Co-Immunoprecipitation, ELISA Capture, ELISA Detection, ELISA Development, ELISA Development (Secondary), Flow Cytometry, Functional Assay, ICC, IF, IHC, IHC-P, Neutralization, Secondary Antibody, Simple Western, Western Blot.


Filter By Application
Bioassay
(2)
Co-Immunoprecipitation
(1)
ELISA Capture
(3)
ELISA Detection
(6)
ELISA Development
(2)
ELISA Development (Secondary)
(1)
Flow Cytometry
(1)
Functional Assay
(1)
ICC
(2)
IF
(1)
IHC
(4)
IHC-P
(2)
Neutralization
(1)
Secondary Antibody
(1)
Simple Western
(1)
Western Blot
(101)
All Applications
Filter By Species
Human
(59)
Mouse
(40)
Rat
(11)
Bat
(1)
Canine
(1)
E. coli
(1)
Macaca mulatta (Rhesus Macaque)
(1)
Mycobacterium smegmatis
(1)
Ovine
(1)
Plasmodium falciparum
(2)
Porcine
(1)
Primate - C. aethiops
(1)
Primate - Macaca fascicularis (Cynomolgus Macaque)
(1)
Transgenic Mouse
(1)
Xenograft
(2)
Yeast
(1)
Yeast - Saccharomyces cerevisiae
(1)
All Species
Showing Publications 1 - 10 of 248. Show All 248 Publications.
Publications using HAF007 Applications Species
Ekchariyawat, P;Saengfak, R;Sanongkiet, S;Charoenwongpaiboon, T;Khongpraphan, S;Mala, S;Luangjindarat, C;Munyoo, B;Chabang, N;Charoensutthivarakul, S;Borwornpinyo, S;Tuchinda, P;Ponpuak, M;Pudla, M;Utaisincharoen, P; ECDD-S16 targets vacuolar ATPase: A potential inhibitor compound for pyroptosis-induced inflammation PloS one 2023-11-27 [PMID: 38011122] (Western Blot, Mouse) Western Blot Mouse
Terry L. Hafer, Abby Felton, Yennifer Delgado, Harini Srinivasan, Michael Emerman A CRISPR Screen of HIV Dependency Factors Reveals That CCNT1 Is Non-Essential in T Cells but Required for HIV-1 Reactivation from Latency Viruses 8/31/2023 [PMID: 37766271]
Huang, Y;Cuan, X;Zhu, W;Yang, X;Zhao, Y;Sheng, J;Zi, C;Wang, X; An EGCG Derivative in Combination with Nimotuzumab for the Treatment of Wild-Type EGFR NSCLC International journal of molecular sciences 2023-09-13 [PMID: 37762316] (Western Blot, Xenograft, Human) Western Blot Xenograft, Human
Heejin Lee, Hye-Ji Kim, Dong-Kyu Choi, Eu n.‐A. Ko, Jae-Hyeog Choi, Yohan Seo et al. Dopaminergic cell protection and alleviation of neuropsychiatric disease symptoms by VMAT2 expression through the class I HDAC inhibitor TC-H 106 Pharmacol Res Perspect 9/23/2023 [PMID: 37740715]
Marina Barrio-Calvo, Søren Vester Kofoed, Sofie Cens Holste, Anders Bundgård Sørensen, Nadia Viborg, Jens Vindahl Kringelum et al. Targeting neoantigens to APC-surface molecules improves the immunogenicity and anti-tumor efficacy of a DNA cancer vaccine Frontiers in Immunology 8/29/2023 [PMID: 37720215]
Huang, Y;Zhu, L;Cheng, S;Dai, R;Huang, C;Song, Y;Peng, B;Li, X;Wen, J;Gong, Y;Hu, Y;Qian, L;Zhu, L;Zhang, F;Yu, L;Yi, C;Gu, W;Ling, Z;Ma, L;Tang, W;Peng, L;Shi, G;Zhang, Y;Sun, B; Solar ultraviolet B radiation promotes ?-MSH secretion to attenuate the function of ILC2s via the pituitary-lung axis Nature communications 2023-09-12 [PMID: 37699899] (Western Blot, Transgenic Mouse, Mouse) Western Blot Transgenic Mouse, Mouse
Duy, DL;Kim, N; Yeast transcription factor Msn2 binds to G4 DNA Nucleic acids research 2023-08-24 [PMID: 37615577] (Western Blot, Yeast - Saccharomyces cerevisiae) Western Blot Yeast - Saccharomyces cerevisiae
Xu W, Zhao L. An Enzyme-Linked Immunosorbent Assay for the Detection of Mitochondrial DNA-Protein Cross-Links from Mammalian Cells DNA (Basel) 8/21/2023 [PMID: 37601565]
Hafer, TL;Felton, A;Delgado, Y;Srinivasan, H;Emerman, M; A CRISPR screen of HIV dependency factors reveals CCNT1 is non-essential in T cells but required for HIV-1 reactivation from latency bioRxiv : the preprint server for biology 2023-07-28 [PMID: 37546973] (Western Blot, Human) Western Blot Human
Vitor Teixeira, Ibrahim Mohamed, Jean-Claude Lavoie Disturbances of the Lung Glutathione System in Adult Guinea Pigs Following Neonatal Vitamin C or Cysteine Deficiency Antioxidants (Basel) 6/29/2023 [PMID: 37507901]
Show All 248 Publications.

Reviews for IgG Antibody (HAF007) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using HAF007:
Filter by Applications
ELISA
(2)
WB
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
ELISA Mouse 08/12/2023
View

Summary

ApplicationELISA
Sample Testedmouse serum
SpeciesMouse
LotFIM3221081
  4
reviewed by:
Verified Customer
ELISA Mouse 08/12/2023
View

Summary

ApplicationELISA
Sample TestedSerum
SpeciesMouse
LotFIM3221081
  5
reviewed by:
Verified Customer
WB Human 04/15/2021
View

Summary

ApplicationWestern Blot
Sample TestedHEK293T and NIH3T3 whole cell lysate
SpeciesHuman
LotFIM3120081

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IgG Antibody (HAF007) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IgG Products

Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Verified Customer
08/12/2023
Application: ELISA
Species: Mouse

Verified Customer
08/12/2023
Application: ELISA
Species: Mouse

Verified Customer
04/15/2021
Application: WB
Species: Human

Bioinformatics

Gene Symbol IGHG1