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Mouse IL-6 Quantikine ELISA Kit, 2 Plate

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Increased production of proinflammatory cytokines and VEGF in the plasma and chondrocytes in high fat diet (HFD)-feeding mice.(a~d) levels of plasma IL-6 (a), TNF-alpha (b), IL-1 beta (c) and VEGF (d) in the C57BL/6 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Catalog# & Conjugate Size Price
HRP
PM6000B
HRP
SM6000B
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Mouse IL-6 Quantikine ELISA Kit, 2 Plate Summary

Background
The Quantikine Mouse IL-6 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse IL-6 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-6 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse IL-6. Results obtained using natural mouse IL-6 sho...wed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-6.
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Additional Information
2 plate pack size in process of discontinuation
Specificity
Natural and recombinant mouse IL-6
Source
N/A
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Il6

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
M6000B in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-6 Quantikine ELISA Kit, 2 Plate

  • B-cell differentiation factor
  • B-cell stimulatory factor 2
  • BSF2
  • BSF-2
  • CDF
  • CTL differentiation factor
  • HSF
  • hybridoma growth factor
  • IFNB2
  • IFN-beta-2
  • IL6
  • IL-6
  • Interferon beta-2
  • interleukin 6 (interferon, beta 2)
  • interleukin BSF-2
  • interleukin-6
  • MGI-2A

Background

Interleukin 6 (IL-6) is a pleiotropic, a-helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length and shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. Cells known to express IL-6 include CD8+ T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelins), sympathetic neurons, cerebral cortex neurons, adrenal medulla chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells and pancreatic islet beta cells. IL-6 production is generally correlated with cell activation and is normally kept in control by glucocorticoids, catecholamines, and secondary sex steroids. Normal human circulating IL-6 is in the 1 pg/mL range, with slight elevations during the menstrual cycle, modest elevations in certain cancers, and large elevations after surgery.

IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 Ra, triggering IL-6 Ra association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 Ra are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 Ra elicit responses from gp130-expressing cells that lack cell surface IL-6 Ra. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 Ra is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 Ra but not from other cytokines that use gp130 as a co-receptor. 

IL-6, along with TNF-a and IL-1, drives the acute inflammatory response. IL-6 is almost solely responsible for fever and the acute phase response in the liver, and it is important in the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in conditions such as obesity, insulin resistance, inflammatory bowel disease, arthritis, and sepsis. IL-6 modulates bone resorption and is a major effector of inflammatory joint destruction in rheumatoid arthritis through its promotion of Th17 cell development and activity. It contributes to atherosclerotic plaque development and destabilization as well as the development of inflammation-associated carcinogenesis.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-6 (M6000B)(629)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Egyptian spiny mouse, Mouseq, Transgenic Mouse, Xenograft.

We have publications tested in 1 application: ELISA Capture.


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ELISA Capture
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Human
(5)
Mouse
(617)
Rat
(1)
Egyptian spiny mouse
(1)
Mouseq
(1)
Transgenic Mouse
(6)
Xenograft
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Showing Publications 1 - 10 of 629. Show All 629 Publications.
Publications using M6000B Applications Species
Chung, YC;Song, SJ;Lee, A;Jang, CH;Kim, CS;Hwang, YH; Isobavachin, a main bioavailable compound in Psoralea corylifolia, alleviates lipopolysaccharide-induced inflammatory responses in macrophages and zebrafish by suppressing the MAPK and NF-?B signaling pathways Journal of ethnopharmacology 2023-11-25 [PMID: 38012970] (Mouse) Mouse
Lee, SG;Park, CH;Kang, H; Effect of E. cava and C. indicum Complex Extract on Phorbol 12-Myristate 13-Acetate (PMA)-Stimulated Inflammatory Response in Human Pulmonary Epithelial Cells and Particulate Matter (PM)2.5-Induced Pulmonary Inflammation in Mice Pharmaceutics 2023-11-13 [PMID: 38004599] (Mouse) Mouse
Haller, AM;Wolfkiel, PR;Jaeschke, A;Hui, DY; Inactivation of Group 1B Phospholipase A2 Enhances Disease Recovery and Reduces Experimental Colitis in Mice International journal of molecular sciences 2023-11-10 [PMID: 38003345] (Mouse) Mouse
Xu, M;Shi, F;Gao, Y;Han, S;Huang, C;Hou, Q;Wen, X;Wang, B;Zhu, Z;Zou, L;Xiong, M;Dong, W;Tan, J; Arabinose confers protection against intestinal injury by improving integrity of intestinal mucosal barrier International immunopharmacology 2023-11-22 [PMID: 37995573] (Mouse) Mouse
Elean, M;Raya Tonetti, F;Fukuyama, K;Arellano-Arriagada, L;Namai, F;Suda, Y;Gobbato, N;Nishiyama, K;Villena, J;Kitazawa, H; Immunobiotic Ligilactobacillus salivarius FFIG58 Confers Long-Term Protection against Streptococcus pneumoniae International journal of molecular sciences 2023-10-30 [PMID: 37958756] (Mouse) Mouse
Chen, F;Xiong, BR;Xian, SY;Zhang, J;Ding, RW;Xu, M;Zhang, ZZ; Fibroblast growth factor 5 protects against spinal cord injury through activating AMPK pathway Journal of cellular and molecular medicine 2023-11-10 [PMID: 37950418] (Mouse) Mouse
Wang, J;Ling, D;Shi, L;Li, H;Peng, M;Wen, H;Liu, T;Liang, R;Lin, Y;Wei, L;Zhang, G;Chen, S; METTL3-mediated m6A methylation regulates ovarian cancer progression by recruiting myeloid-derived suppressor cells Cell & bioscience 2023-11-06 [PMID: 37932814] (Mouse, Transgenic Mouse) Mouse, Transgenic Mouse
Ur Rahim, J;Faheem, MM;Nawaz, S;Goswami, A;Rai, R; Synthesis and characterization of piperic acid conjugates with homochiral and heterochiral dipeptides containing phenylalanine and their application in skin cancer Peptides 2023-11-03 [PMID: 37923167] (Mouse) Mouse
Sun, C;Peng, S;Lv, Z;Guo, T;Zhang, L; Research of STEAP3 interaction with Rab7A and RACK1 to modulate the MAPK and JAK/STAT signaling in Osteoarthritis International immunopharmacology 2023-10-09 [PMID: 37820423] (Rat) Rat
Hou, C;Jiang, X;Sheng, W;Zhang, Y;Lin, Q;Hong, S;Zhao, J;Wang, T;Ye, X; Xinmaikang (XMK) tablets alleviate atherosclerosis by regulating the SREBP2-mediated NLRP3/ASC/Caspase-1 signaling pathway Journal of ethnopharmacology 2023-09-28 [PMID: 37777030] (Mouse) Mouse
Show All 629 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-6 (M6000B). (Showing 1 - 1 of 1 FAQs).

  1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
    • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

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Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.
Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.
The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein ...  Read full blog post.
The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.
VEGF: Vascular endothelial growth factor
VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g...  Read full blog post.
TLR2 - I can recognize many foreign pathogens!
TLR2 is a member of the broad family of Toll-like receptors (TLR) that play an important role in pathogen recognition and innate immunity. TLRs, like other pattern recognition receptors (PRRs), recognize endogenous ligands released during cell or tiss...  Read full blog post.
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Bioinformatics

Gene Symbol Il6