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Mouse IL-6 Quantikine ELISA Kit, 2 Plate

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Increased production of proinflammatory cytokines and VEGF in the plasma and chondrocytes in high fat diet (HFD)-feeding mice.(a~d) levels of plasma IL-6 (a), TNF-alpha (b), IL-1 beta (c) and VEGF (d) in the C57BL/6 ...read more
PYC suppressed LPS-induced proinflammatory cytokine production in BV2 microglia.Cells were incubated with the indicated concentrations of PYC or vehicle for 1h before LPS treatment (500ng/mL). After 24 h incubation, the ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Mouse IL-6 Quantikine ELISA Kit, 2 Plate Summary

Background
The Quantikine Mouse IL-6 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse IL-6 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-6 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse IL-6. Results obtained using natural mouse IL-6 sho...wed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-6.
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Additional Information
2 plate pack size in process of discontinuation
Specificity
Natural and recombinant mouse IL-6
Source
N/A
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Il6

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
M6000B in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-6 Quantikine ELISA Kit, 2 Plate

  • B-cell differentiation factor
  • B-cell stimulatory factor 2
  • BSF2
  • BSF-2
  • CDF
  • CTL differentiation factor
  • HSF
  • hybridoma growth factor
  • IFNB2
  • IFN-beta-2
  • IL6
  • IL-6
  • Interferon beta-2
  • interleukin 6 (interferon, beta 2)
  • interleukin BSF-2
  • interleukin-6
  • MGI-2A

Background

Interleukin 6 (IL-6) is a pleiotropic, a-helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length and shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. Cells known to express IL-6 include CD8+ T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelins), sympathetic neurons, cerebral cortex neurons, adrenal medulla chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells and pancreatic islet beta cells. IL-6 production is generally correlated with cell activation and is normally kept in control by glucocorticoids, catecholamines, and secondary sex steroids. Normal human circulating IL-6 is in the 1 pg/mL range, with slight elevations during the menstrual cycle, modest elevations in certain cancers, and large elevations after surgery.

IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 Ra, triggering IL-6 Ra association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 Ra are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 Ra elicit responses from gp130-expressing cells that lack cell surface IL-6 Ra. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 Ra is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 Ra but not from other cytokines that use gp130 as a co-receptor. 

IL-6, along with TNF-a and IL-1, drives the acute inflammatory response. IL-6 is almost solely responsible for fever and the acute phase response in the liver, and it is important in the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in conditions such as obesity, insulin resistance, inflammatory bowel disease, arthritis, and sepsis. IL-6 modulates bone resorption and is a major effector of inflammatory joint destruction in rheumatoid arthritis through its promotion of Th17 cell development and activity. It contributes to atherosclerotic plaque development and destabilization as well as the development of inflammation-associated carcinogenesis.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-6 (M6000B)(655)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Mouseq, Transgenic Mouse, Xenograft.

We have publications tested in 1 application: ELISA Capture.


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ELISA Capture
(5)
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Human
(6)
Mouse
(643)
Rat
(1)
Mouseq
(1)
Transgenic Mouse
(9)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 655. Show All 655 Publications.
Publications using M6000B Applications Species
Song, JY;Lee, YJ;Lee, SH;Lee, JY; Enoximone alleviates atopic dermatitis-like skin inflammation via inhibition of type 2 T helper cell development International immunopharmacology 2024-09-17 [PMID: 39293315] (Mouse) Mouse
Xie, F;Kitagawa, Y;Ogata, H;Yasuhara, S;You, Z;Jeevendra Martyn, JA; Morphine induces inflammatory responses via both TLR4 and cGAS-STING signaling pathways Cytokine 2024-08-31 [PMID: 39217915] (Mouse) Mouse
Ben-Crentsil, NA;Mohammed Ismail, W;Balasis, ME;Newman, H;Quintana, A;Binder, M;Kruer, T;Neupane, S;Ferrall-Fairbanks, MC;Fernandez, J;Lasho, TL;Finke, CM;Ibrahim, ML;McGraw, KL;Wysota, M;Aldrich, AL;Ryder, CB;Letson, CT;Traina, J;McLemore, AF;Droin, N;Shastri, A;Yun, S;Solary, E;Sallman, DA;Beg, AA;Ma, L;Gaspar-Maia, A;Patnaik, MM;Padron, E; RNA shielding of P65 is required to potentiate oncogenic inflammation in TET2 mutated clonal hematopoiesis Cancer discovery 2024-08-27 [PMID: 39189614] (Mouse) Mouse
Bernard, A;Eggstein, C;Tang, L;Keller, M;Körner, A;Mirakaj, V;Rosenberger, P; Plexin C1 influences immune response to intracellular LPS and survival in murine sepsis Journal of biomedical science 2024-08-21 [PMID: 39169397] (Transgenic Mouse) Transgenic Mouse
Su, J;Chen, K;Sang, X;Feng, Z;Zhou, F;Zhao, H;Wu, S;Deng, X;Lin, C;Lin, X;Xie, L;Ye, H;Chen, Q; Huperzine a ameliorates sepsis-induced acute lung injury by suppressing inflammation and oxidative stress via ?7 nicotinic acetylcholine receptor International immunopharmacology 2024-08-18 [PMID: 39159557] (Mouse) Mouse
Su, J;Xiao, J;Deng, X;Lin, X;Xie, L;Ye, H;Lin, C;Zhou, F;Wu, S; Combining Aloin with TIENAM ameliorates cecal ligation and puncture-induced sepsis in mice by attenuating inflammation and modulating abdominal cavity microbiota International immunopharmacology 2024-08-17 [PMID: 39154534] (Mouse) Mouse
Shin, JY;Cho, BO;Park, JH;Kang, ES;Kim, JH;Ha, HY;Kim, YS;Jang, SI; Diospyros lotus leaf extract and its main component myricitrin inhibit itch?related IL?6 and IL?31 by suppressing microglial inflammation and microglial?mediated astrocyte activation Molecular medicine reports 2024-10-01 [PMID: 39129303] (Mouse) Mouse
Liu, X;Cui, L;Tao, Y;Xia, S;Hou, J;Cao, X;Xu, S; The deubiquitinase BAP1 and E3 ligase UBE3C sequentially target IRF3 to activate and resolve the antiviral innate immune response Cell reports 2024-08-08 [PMID: 39120972] (Mouse) Mouse
Tian, R;Li, R;Chen, Y;Liu, D;Li, Y;He, S;Pan, T;Qu, H;Tan, R; Shenfu injection ameliorates endotoxemia-associated endothelial dysfunction and organ injury via inhibiting PI3K/Akt-mediated glycolysis Journal of ethnopharmacology 2024-07-30 [PMID: 39089657] (Mouse) Mouse
Widjaja, AA;Lim, WW;Viswanathan, S;Chothani, S;Corden, B;Dasan, CM;Goh, JWT;Lim, R;Singh, BK;Tan, J;Pua, CJ;Lim, SY;Adami, E;Schafer, S;George, BL;Sweeney, M;Xie, C;Tripathi, M;Sims, NA;Hübner, N;Petretto, E;Withers, DJ;Ho, L;Gil, J;Carling, D;Cook, SA; Inhibition of IL-11 signalling extends mammalian healthspan and lifespan Nature 2024-07-17 [PMID: 39020175] (Mouse, Transgenic Mouse) Mouse, Transgenic Mouse
Show All 655 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-6 (M6000B). (Showing 1 - 1 of 1 FAQs).

  1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
    • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

Additional IL-6 Products

Blogs on IL-6. Showing 1-10 of 12 blog posts - Show all blog posts.


  Read full blog post.


  Read full blog post.

Rest in Peace: Is the Receptor Interacting Protein (RIP) Kinase-3 (RIPK3) a Protector or Offender?
By Jamshed Arslan, Pharm. D., PhD. Receptor interacting protein kinases (RIPKs) in necroptosisDeath is perhaps inevitable. Cell death can be programed and immunologically silent (apoptosis), unprogrammed and inflamm...  Read full blog post.


  Read full blog post.

Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.

Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein ...  Read full blog post.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

VEGF: Vascular endothelial growth factor
VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g...  Read full blog post.

TLR2 - I can recognize many foreign pathogens!
TLR2 is a member of the broad family of Toll-like receptors (TLR) that play an important role in pathogen recognition and innate immunity. TLRs, like other pattern recognition receptors (PRRs), recognize endogenous ligands released during cell or tiss...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.
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Bioinformatics

Gene Symbol Il6