Reactivity | Hu, Mu, Rt, CaSpecies Glossary |
Applications | WB, Simple Western, Flow, ICC/IF, IHC, IP |
Description | This pack contains 1 vial each of: NB100-654SS (0.025 mL), NB100-479SS (0.025 mL), NB100-449SS (0.025 mL), NB100-105 (0.05 mL) and NB100-131 (0.05 mL). |
Immunogen | For NB 100-654: Human HIF-1 alpha amino acids 432-528. For NB 100-105: Human HIF-1 alpha amino acids 432-528. For NB 100-131: Human HIF-1 alpha amino acids 329-530. For NB 100-449: Human HIF-1 alpha amino acids 775-826. For NB 100-479: Antibody was developed against a fusion protein including amino acids 530 - 825 of the mouse HIF-1 alpha protein [Uniprot# Q61221]. |
Specificity | These antibodies are specific for HIF-1alpha. |
Gene | HIF1A |
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Application Notes | See individual datasheets of components for their validated applications. Western Blot: A starting dilution of 1:1,000 is recommended with titration up or down depending upon the signal and background. Immunohistochemistry: Both positive and negative feedback have been provided on all antibodies included in this pack. A starting dilution of 1:250 is recommended with titration up or down depending upon the signal and background. |
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Storage | Store at 4C. Do not freeze. |
Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Ming Lu |
WB | Human | 08/08/2014 |
Summary
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Enlarge |
reviewed by:
Ming Lu |
WB | Human | 08/05/2014 |
Summary
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Research Areas for HIF-1 alpha Antibody Pack (NB100-905)Find related products by research area.
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Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ... Read full blog post. |
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Read full blog post. |
Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax... Read full blog post. |
mTOR Signaling and the Tumor Microenvironment By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ... Read full blog post. |
Bad news for stomach cancer: BAMBI protein inhibits gastric carcinoma via TGF-beta/epithelial-mesenchymal transition signaling By Jamshed Arslan Pharm.D. Gastric carcinoma is the second leading cause of cancer-related deaths worldwide. One of the key features of gastric carcinoma is acidosis, which promotes growth and metastasis of gastric ... Read full blog post. |
Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto... Read full blog post. |
Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy... Read full blog post. |
Forecasting and Targeting a Rare Cancer with Hypoxia-Inducible Factor By Jamshed Arslan Pharm.D. Cancers of nerve, adipose, and other soft tissues are called soft tissue sarcomas (STS). Malignant peripheral nerve sheath tumor (MPNST) is an example of a rare and hard-to-treat STS; eve... Read full blog post. |
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Ming Lu 08/08/2014 |
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Application: | WB | |
Species: | Human |
Ming Lu 08/05/2014 |
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Application: | WB | |
Species: | Human |
Gene Symbol | HIF1A |