Using the Hif-1 Alpha Antibody in Prostate Cancer Research

The Hypoxia-inducible Factor 1 (HIF1) protein is a heterodimeric transcription factor which plays an important role in mammalian oxygen homeostasis in conditions of hypoxia, or low oxygen concentration. HIF-1 alpha antibody reagents are widely used in disease research, as hypoxia is known to contribute to many diseases including cancer, pulmonary hypertension and congenital heart disease. We at Novus Biologicals are one of the leading antibody suppliers of HIF-1 products.

HIF-1 alpha is one of two subunits forming the HIF-1 heterodimer. Under normal conditions it undergoes continual translation, ubiquitination and degradation, but in hypoxic conditions degradation ceases while transcription increases. Expression of HIF-1 alpha is regulated by several enzymes, including the E3 ubiquitin-protein ligase Siah2.

HIF-1 regulates a number of genes known to be over expressed in certain tumours. Recently J.Qi et al, of the Sanford-Burnham Medical Research Institute, recruited the HIF-1 alpha antibody in a research study which threw light on the role of HIF-1 and Siah2 in the formation of the neuroendocrine (NE) phenotype and aggressive NE prostate tumors. The study used the TRAMP prostate cancer mouse model.

It was seen that prostate tumor formation was suppressed in those mice deficient in Siah2. In normal mice, HIF-1alpha interacted with FoxA2, a transcription factor widely expressed in neuroendocrine tissue, promoting recruitment of the transcriptional co-activator p300. This selectively activated the HIF-regulated genes, Sox9, Hes6, and Jmjd1a. These proteins are highly expressed in metastatic prostate adenocarcinomas (PCas), PCa metastasis, NE tumor formation and the NE phenotype – which is seen in around 30% of NE tumors.

Novus is a worldwide leader in hypoxia research reagents, offering a highly validated line of top quality antibodies,hypoxic lysates, and much more.

Novus Biologicals offers many HIF-1 alpha reagents for your research needs including:

• HIF-1 alpha Antibodies
• HIF-1 alpha Lysates
• HIF-1 alpha RNAi
• HIF-1 alpha Kits
• HIF-1 alpha Antibody Pairs
• HIF-1 alpha Antibody Packs
• HIF-1 alpha Peptides and Proteins