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Goat anti-Rabbit IgG Secondary Antibody [HRP]

Images

 
Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60 Polyclonal Antibody (AF1800) using a 1:1000 dilution of HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was ...read more
Detection of FoxP3 in human tonsil using 5 μg/mL of Rabbit monoclonal anti-human FoxP3 primary antibody (MAB8214) followed by 30 min incubation with Goat anti Rabbit HRP conjugated secondary antibodies (Catalog # ...read more
PARP-cleavage upon treatment with embryonic stimuli.Representative Western Blot analysis of PARP cleavage (full length PARP 116kDa; cleaved PARP 89 kDa) in non-differentiated (St-T1, lanes 1+2; KdS1, lanes 3+4) and ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
HRP

Goat anti-Rabbit IgG Secondary Antibody [HRP] Summary

Immunogen
Rabbit IgG
Specificity
Detects rabbit IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with human IgG, mouse IgG, and chicken IgY is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Western Blot
Reviewed Applications
Read 1 Review rated 5
using
HAF008 in the following applications:

Publications
Read Publications using
HAF008 in the following applications:

Packaging, Storage & Formulations

Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied as a 0.2 um filtered solution in Sodium Phosphate and Proclin 300.
Preservative
Proclin 300

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat anti-Rabbit IgG Secondary Antibody [HRP]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (HAF008)(266)

We have publications tested in 17 confirmed species: Human, Mouse, Rat, Bacteria - E. Coli, Bovine, Canine, Frog - Xenopus (African Clawed Frog), Galling Sawfly, Insect - Aedes albopictus (Asian tiger mosquito), Porcine, Primate - Callithrix jacchus (Common Marmoset), Primate - Chlorocebus aethiops (African Green Monkey), Primate - Macaca nemestrina (Southern Pig-tailed Macaque), Rabbit, Transgenic Mouse, Xenograft, Yeast.

We have publications tested in 12 applications: Bioassay, Co-IP, ELISA Development, Flow Cytometry, Functional Assay, ICC, IHC, IHC-P, Immunohistochemistry, Secondary Antibody, Simple Western, Western Blot.


Filter By Application
Bioassay
(1)
Co-IP
(1)
ELISA Development
(1)
Flow Cytometry
(1)
Functional Assay
(1)
ICC
(1)
IHC
(5)
IHC-P
(1)
Immunohistochemistry
(1)
Secondary Antibody
(1)
Simple Western
(1)
Western Blot
(116)
All Applications
Filter By Species
Human
(57)
Mouse
(48)
Rat
(12)
Bacteria - E. Coli
(1)
Bovine
(2)
Canine
(1)
Frog - Xenopus (African Clawed Frog)
(1)
Galling Sawfly
(1)
Insect - Aedes albopictus (Asian tiger mosquito)
(1)
Porcine
(1)
Primate - Callithrix jacchus (Common Marmoset)
(1)
Primate - Chlorocebus aethiops (African Green Monkey)
(1)
Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
(1)
Rabbit
(1)
Transgenic Mouse
(2)
Xenograft
(1)
Yeast
(1)
All Species
Showing Publications 1 - 10 of 266. Show All 266 Publications.
Publications using HAF008 Applications Species
Seguchi, M;Yamaguchi, S;Tanaka, M;Mori, Y;Tsurudome, M;Ito, M; Effects of Alkaline Solutions on the Structure and Function of Influenza A Virus Viruses 2024-10-19 [PMID: 39459968] (Western Blot, Bovine) Western Blot Bovine
Guajardo-Contreras, GI;Dos-Santos, ALA;Chen, A;Niu, M;Beauchamp, E;Berthiaume, LG;Cochane, AW;Mouland, AJ; HIV-1 N-myristoylation-dependent hijacking of late endosomes/lysosomes to drive Gag assembly in macrophages Journal of cell science 2024-10-23 [PMID: 39439384] (Western Blot, Human) Western Blot Human
Song, B;Korolkova, O; Bafilomycin A1 Inhibits HIV-1 Infection by Disrupting Lysosomal Cholesterol Transport Viruses 2024-08-29 [PMID: 39339852] (Western Blot, Human) Western Blot Human
Pena Calderin, EH;Zheng, JJ;Boyd, NL;Lynch, W;Sansbury, BE;Spite, M;Hill, BG;Hellmann, J; Exercise-stimulated Resolvin Biosynthesis in Adipose Tissue is Abrogated by High Fat Diet-induced Adrenergic Deficiency bioRxiv : the preprint server for biology 2024-08-19 [PMID: 39229191] (Western Blot, Mouse) Western Blot Mouse
Bhattacharya, T;Alleman, EM;Noyola, AC;Emerman, M;Malik, HS; A conserved opal termination codon optimizes a temperature-dependent tradeoff between protein production and processing in alphaviruses bioRxiv : the preprint server for biology 2024-08-22 [PMID: 39229031] (Western Blot, Primate - Chlorocebus aethiops (African Green Monkey), Insect - Aedes albopictus (Asian tiger mosquito)) Western Blot Primate - Chlorocebus aethiops (African Green Monkey), Insect - Aedes albopictus (Asian tiger mosquito)
Schulthei beta , C;Paschold, L;Mohebiany, AN;Escher, M;Kattimani, YM;Müller, M;Schmidt-Barbo, P;Mensa-Vilaró, A;Aróstegui, JI;Boursier, G;de Moreuil, C;Hautala, T;Willscher, E;Jonas, H;Chinchuluun, N;Grosser, B;Märkl, B;Klapper, W;Oommen, PT;Gössling, K;Hoffmann, K;Tiegs, G;Czernilofsky, F;Dietrich, S;Freeman, A;Schwartz, DM;Waisman, A;Aksentijevich, I;Binder, M; A20 haploinsufficiency disturbs immune homeostasis and drives the transformation of lymphocytes with permissive antigen receptors Science advances 2024-08-23 [PMID: 39167656] (Western Blot, Human) Western Blot Human
Khongpraphan, S;Ekchariyawat, P;Sanongkiet, S;Luangjindarat, C;Sirisinha, S;Ponpuak, M;Midoeng, P;Pudla, M;Utaisincharoen, P; Differentiation in pyroptosis induction by Burkholderia pseudomallei and Burkholderia thailandensis in primary human monocytes, a possible cause of sepsis in acute melioidosis patients PLoS neglected tropical diseases 2024-07-01 [PMID: 39042701] (Western Blot, Human) Western Blot Human
Barbu, E;Mihaila, AC;Gan, AM;Ciortan, L;Macarie, RD;Tucureanu, MM;Filippi, A;Stoenescu, AI;Petrea, SV;Simionescu, M;Balanescu, SM;Butoi, E; The Elevated Inflammatory Status of Neutrophils Is Related to In-Hospital Complications in Patients with Acute Coronary Syndrome and Has Important Prognosis Value for Diabetic Patients International journal of molecular sciences 2024-05-08 [PMID: 38791147] (Western Blot, Human) Western Blot Human
Guo, T;Pei, F;Zhang, M;Yamada, T;Feng, J;Jing, J;Ho, TV;Chai, Y; Vascular architecture regulates mesenchymal stromal cell heterogeneity via P53-PDGF signaling in the mouse incisor Cell stem cell 2024-04-30 [PMID: 38703771] (Western Blot, Transgenic Mouse) Western Blot Transgenic Mouse
Pera, EM;Nilsson-De Moura, J;Pomeshchik, Y;Roybon, L;Milas, I; Inhibition of the serine protease HtrA1 by SerpinE2 suggests an extracellular proteolytic pathway in the control of neural crest migration eLife 2024-04-18 [PMID: 38634469] (Western Blot, Human, Frog - Xenopus (African Clawed Frog)) Western Blot Human, Frog - Xenopus (African Clawed Frog)
Show All 266 Publications.

Review for IgG Antibody (HAF008) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human and Pig.

Reviews using HAF008:
Filter by Applications
Simple Western
(1)
All Applications
Filter by Species
Human and Pig
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western IgG HAF008
Enlarge
5
reviewed by:
Verified Customer
Simple Western Human and Pig 08/14/2019
View

Summary

ApplicationSimple Western
Sample Testedhuman liver cells, Sample Amount: 15 ug,Liver tissue,Muscle tissue,Skin tissue
SpeciesHuman and Pig
LotFIN1819021

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IgG Antibody (HAF008). (Showing 1 - 1 of 1 FAQs).

  1. May I know the buffer used in this product?
    • It is supplied as a 0.2um filtered solution in sodium phosphate and Proclin 300

Additional IgG Products

Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Recent Reviews

5
5
1
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1
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Verified Customer
08/14/2019
Application: Simple Western
Species: Human and Pig

Bioinformatics

Gene Symbol IGHG1