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Goat anti-Rabbit IgG Secondary Antibody [HRP]

Images

 
Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60 Polyclonal Antibody (AF1800) using a 1:1000 dilution of HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was ...read more
Detection of FoxP3 in human tonsil using 5 μg/mL of Rabbit monoclonal anti-human FoxP3 primary antibody (MAB8214) followed by 30 min incubation with Goat anti Rabbit HRP conjugated secondary antibodies (Catalog # ...read more
PARP-cleavage upon treatment with embryonic stimuli.Representative Western Blot analysis of PARP cleavage (full length PARP 116kDa; cleaved PARP 89 kDa) in non-differentiated (St-T1, lanes 1+2; KdS1, lanes 3+4) and ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
HRP

Goat anti-Rabbit IgG Secondary Antibody [HRP] Summary

Immunogen
Rabbit IgG
Specificity
Detects rabbit IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with human IgG, mouse IgG, and chicken IgY is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Western Blot
Reviewed Applications
Read 1 Review rated 5
using
HAF008 in the following applications:

Publications
Read Publications using
HAF008 in the following applications:

Packaging, Storage & Formulations

Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied as a 0.2 um filtered solution in Sodium Phosphate and Proclin 300.
Preservative
Proclin 300

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat anti-Rabbit IgG Secondary Antibody [HRP]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (HAF008)(252)

We have publications tested in 14 confirmed species: Human, Mouse, Rat, Bacteria - E. Coli, Bovine, Canine, Galling Sawfly, Porcine, Primate - Callithrix jacchus (Common Marmoset), Primate - Macaca nemestrina (Southern Pig-tailed Macaque), Rabbit, Transgenic Mouse, Xenograft, Yeast.

We have publications tested in 11 applications: Bioassay, Co-IP, ELISA Development, Flow Cytometry, Functional Assay, ICC, IHC, IHC-P, Secondary Antibody, Simple Western, Western Blot.


Filter By Application
Bioassay
(1)
Co-IP
(1)
ELISA Development
(1)
Flow Cytometry
(1)
Functional Assay
(1)
ICC
(1)
IHC
(6)
IHC-P
(1)
Secondary Antibody
(1)
Simple Western
(1)
Western Blot
(105)
All Applications
Filter By Species
Human
(51)
Mouse
(47)
Rat
(11)
Bacteria - E. Coli
(1)
Bovine
(1)
Canine
(1)
Galling Sawfly
(1)
Porcine
(1)
Primate - Callithrix jacchus (Common Marmoset)
(1)
Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
(1)
Rabbit
(1)
Transgenic Mouse
(1)
Xenograft
(1)
Yeast
(1)
All Species
Showing Publications 1 - 10 of 252. Show All 252 Publications.
Publications using HAF008 Applications Species
Eshchenko, N;Sergeeva, M;Zhuravlev, E;Kudria, K;Goncharova, E;Komissarov, A;Stepanov, G; A Knockout of the IFITM3 Gene Increases the Sensitivity of WI-38 VA13 Cells to the Influenza A Virus International journal of molecular sciences 2024-01-03 [PMID: 38203797] (Western Blot, Human) Western Blot Human
Nakanishi, R;Tanaka, M;Nisa, BU;Shimizu, S;Hirabayashi, T;Tanaka, M;Maeshige, N;Roy, RR;Fujino, H; Alternating current electromagnetic field exposure lessens intramyocellular lipid accumulation due to high-fat feeding via enhanced lipid metabolism in mice PloS one 2023-11-27 [PMID: 38011220] (Western Blot, Mouse) Western Blot Mouse
Ekchariyawat, P;Saengfak, R;Sanongkiet, S;Charoenwongpaiboon, T;Khongpraphan, S;Mala, S;Luangjindarat, C;Munyoo, B;Chabang, N;Charoensutthivarakul, S;Borwornpinyo, S;Tuchinda, P;Ponpuak, M;Pudla, M;Utaisincharoen, P; ECDD-S16 targets vacuolar ATPase: A potential inhibitor compound for pyroptosis-induced inflammation PloS one 2023-11-27 [PMID: 38011122] (Western Blot, Mouse) Western Blot Mouse
Terry L. Hafer, Abby Felton, Yennifer Delgado, Harini Srinivasan, Michael Emerman A CRISPR Screen of HIV Dependency Factors Reveals That CCNT1 Is Non-Essential in T Cells but Required for HIV-1 Reactivation from Latency Viruses 8/31/2023 [PMID: 37766271]
Huang, Y;Cuan, X;Zhu, W;Yang, X;Zhao, Y;Sheng, J;Zi, C;Wang, X; An EGCG Derivative in Combination with Nimotuzumab for the Treatment of Wild-Type EGFR NSCLC International journal of molecular sciences 2023-09-13 [PMID: 37762316] (Western Blot, Human, Xenograft) Western Blot Human, Xenograft
Heejin Lee, Hye-Ji Kim, Dong-Kyu Choi, Eu n.‐A. Ko, Jae-Hyeog Choi, Yohan Seo et al. Dopaminergic cell protection and alleviation of neuropsychiatric disease symptoms by VMAT2 expression through the class I HDAC inhibitor TC-H 106 Pharmacol Res Perspect 9/23/2023 [PMID: 37740715]
Huang, Y;Zhu, L;Cheng, S;Dai, R;Huang, C;Song, Y;Peng, B;Li, X;Wen, J;Gong, Y;Hu, Y;Qian, L;Zhu, L;Zhang, F;Yu, L;Yi, C;Gu, W;Ling, Z;Ma, L;Tang, W;Peng, L;Shi, G;Zhang, Y;Sun, B; Solar ultraviolet B radiation promotes ?-MSH secretion to attenuate the function of ILC2s via the pituitary-lung axis Nature communications 2023-09-12 [PMID: 37699899] (Western Blot, Transgenic Mouse, Mouse) Western Blot Transgenic Mouse, Mouse
Inwoo Seo, Sung‐Won Kim, Jiyu Hyun, Yu‐Jin Kim, Hyun Su Park, Jeong‐Kee Yoon et al. Enhancing viability and angiogenic efficacy of mesenchymal stem cells via HSP90 alpha and HSP27 regulation based on ROS stimulation for wound healing Bioengineering & Translational Medicine 6/7/2023 [PMID: 37693062]
Hafer, TL;Felton, A;Delgado, Y;Srinivasan, H;Emerman, M; A CRISPR screen of HIV dependency factors reveals CCNT1 is non-essential in T cells but required for HIV-1 reactivation from latency bioRxiv : the preprint server for biology 2023-07-28 [PMID: 37546973] (Western Blot, Human) Western Blot Human
Momčilović, S;Bogdanović, A;Milošević, MS;Mojsilović, S;Marković, DC;Kočović, DM;Vignjević Petrinović, S; Macrophages Provide Essential Support for Erythropoiesis, and Extracellular ATP Contributes to a Erythropoiesis-Supportive Microenvironment during Repeated Psychological Stress International journal of molecular sciences 2023-07-12 [PMID: 37511129] (Western Blot, Mouse) Western Blot Mouse
Show All 252 Publications.

Review for IgG Antibody (HAF008) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human and Pig.

Reviews using HAF008:
Filter by Applications
Simple Western
(1)
All Applications
Filter by Species
Human and Pig
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western IgG HAF008
Enlarge
5
reviewed by:
Verified Customer
Simple Western Human and Pig 08/14/2019
View

Summary

ApplicationSimple Western
Sample Testedhuman liver cells, Sample Amount: 15 ug,Liver tissue,Muscle tissue,Skin tissue
SpeciesHuman and Pig
LotFIN1819021

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IgG Antibody (HAF008). (Showing 1 - 1 of 1 FAQs).

  1. May I know the buffer used in this product?
    • It is supplied as a 0.2um filtered solution in sodium phosphate and Proclin 300

Additional IgG Products

Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Recent Reviews

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Verified Customer
08/14/2019
Application: Simple Western
Species: Human and Pig

Bioinformatics

Gene Symbol IGHG1