How many units do we need to purchase in order to stain 150 formalin fixed, paraffin embedded kidney biopsies? It depends on the amount used per slide, so could you please confirm how much antibody do you use per slide in IHC-P? Also, is the ab compatible with ENVISION FLEX+, HIGH pH, FOR AS?

Unfortunately there is no number that I can provide to you as it is impossible to know for sure how many slides you will be able to stain with a vial. This will depend on your experimental conditions, your samples and how highly expressed the protein is, and what dilution you end up choosing. This antibody is recommended to be used at a 1:1000 dilution for IHC staining as this has worked well for our lab. You should order a single vial and test to determine what amount will be best for your assay rather than order too much or too little without first working with the antibody. As for your second question, that is not something that our lab has tested with so I am unsure if it is compatible or not. It has a fairly normal range pH in the buffer (7-8) so most likely the functions best around that range and not at a high pH.

Could you please let me know the immunogen peptide location for this Ab?

The immunogen used to create this antibody was made to a cytoplasmic area of the protein.

I'm going to use your CA-9 Antibody for paraffin-Immunohistochemistry. Do I have to do any special pretreatments?

We recommend antigen retrieval to ensure the epitope is not masked, and we also recommend that you add a permeablizing agent to access the epitope in the nucleus. A protocol specific for this product can be found under Product PDFs, labelled as "IHC Protocol".

We dilute our antibodies in 5% milk TBS-Tween and usually freeze-thaw them multiple times. Is this possible with this antibody when diluted?

Freezing is not recommended for NB100-417 under any circumstances. Unfortunately, the antibody would no longer be covered by our guarantee if it was stored at a temperature that did not match our storage instructions. Of course, you are free to test your proposed conditions if you would like, but we would not be able to accept responsibility if the antibody was damaged by this.

Is this antibody is carrier-free? If not, do you have a carrier-free version?

NB100-417 is carrier protein free, but does contain 0.1% sodium azide. The buffer formulation is: Tris-citrate/phosphate, pH 7-8 with 0.1% sodium azide. The following products are carrier protein and azide free (catalog numbers 23300002, H00000768-B01P and H00000768-D01P). If you would like to use NB100-417, we do offer antibody purification kits to remove unwanted buffer ingredients. See this link here for those products.

I have a couple of questions regarding the Carbonic Anhydrase IX Antibody (NB100-417) listed on your website. We are wondering if this antibody is carrier-free, and if not, whether you have a carrier-free version?

NB100-417 is carrier protein free but does contain 0.025% sodium azide. The buffer formulation is: PBS with 0.025% sodium azide. The following products are carrier protein and azide free (catalog numbers NBP2-34411, NBP2-34408, and NBP2-33152). If you would like to use NB100-417, we do offer antibody purification kits to remove unwanted buffer ingredients.

I am interested in using the CA9 antibody (NB100-417) that you supply. We dilute our antibodies in 5% milk TBS-Tween and usually freeze-thaw them multiple times. Is this possible with this antibody when diluted?

I have checked with the lab and they have informed me that freezing is not recommended for NB100-417 under any circumstances. Unfortunately, the antibody would no longer be covered by our guarantee if it was stored at a temperature that did not match our storage instructions. Of course, you are free to test your proposed conditions if you would like, but we would not be able to accept responsibility if the antibody was damaged by this.

Could this antibody (CA9) be safely stored at -80C?

The recommended storage temperature for NB100-417 is -20C. It may be possible to store this antibody at -80C, but this has not been confirmed by our lab. It is most important to ensure that that antibody does not undergo too many freeze-thaw cycles.

Novus Biologicals products are now on bio-techne.com

Carbonic Anhydrase IX/CA9 Antibody - BSA Free

Name: Carbonic Anhydrase IX/CA9 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NB100-417
Rating: 4.4 (7 reviews)

(271 Publications)

(7 Reviews)

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Images

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Enlarge)

CA9 expressed in hypoxic regions as assessed by pimonidazole staining (A), but also observed in non-pimonidazole areas (B). Most of CA9 expressed in pimonidazole positive regions (C). Proliferation (BrdUrd) & apoptosis ...read more

Orthogonal Strategies: Formalin-fixed paraffin-embedded tissue sections of human stomach were probed for Carbonic Anhydrase IX/CA9 mRNA (ACD RNAScope Probe, catalog # 559348; Fast Red chromogen, ACD catalog # ...read more

Analysis on various human cell lysates. Specific bands were detected for Carbonic Anhydrase IX/CA9 in HEK-293 and A498 cell lines at a molecular weight of approximately 50 kDa. WB image submitted by a verified customer ...read more

Immunohistochemical representative microphotographs representing the HIF-1alpha, GLUT-1, and CAIX expression in endometrial cancer according to FIGO classification (IA, IB, II, IIIA, IIIC, and IV). Primary objective ...read more

Biological Strategies: Hypoxias impact on proteins of the mitochondrial ISC assembly machinery. (A) Immunoblotting analyzed the total protein extracts from HeLa cells grown in normoxia (Nx, 21% O2) or hypoxia ...read more

Biological Strategies: Analysis using the DyLight 488 conjugate of NB100-417. Staining of Carbonic Anhydrase IX (red) in human glioma U87 cells. DAPI counterstains nuclei (blue). ICC/IF image submitted by a ...read more

Simple Western lane view shows a specific band for CAIX in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

An intracellular stain was performed on U-87 MG Cells with NB100-417 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of ...read more

Western Blot analysis of Carbonic Anhydrase IX/CA9 Antibody on human breast cancer MCF7 cells. Image from verified customer review.

A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Carbonic Anhydrase IX/CA9 Antibody NB100-417 at 2 ug/ml ...read more

An intracellular stain was performed on A431 cells with Carbonic Anhydrase IX/CA9 Antibody NB100-417 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. ...read more

Analysis in 1) HeLa, 2) MDA-MB-231, and 3) A549 whole cell lysates. Specific bands were detected for Carbonic Anhydrase IX/CA9 at a molecular weight of 50 kDa.

Analysis on rat renal cortex. A specific band was detected at a molecular weight of approximately 50 kDa.

Immunocytochemical analysis of CNHCs with antibodies against the RCC marker CAIX. Clusters of CNHCs cytomorphologically classified as uncertain malignant (-UMF) with cytoplasmic positive staining with antibodies against ...read more

Analysis of human colon carcinoma, xenografted in mice. IHC-Fr image submitted by a verified customer review.

IHC analysis of a FFPE tissue section of human breast cancer using CAIX antibody at 1:1000 dilution. The primary antibody bound to CAIX antigens in the tissue section was detected using a HRP labeled secondary antibody ...read more

Strong cytoplasmic and membranous CA9 staining in glandular cells of human stomach tissue. Citrate buffer pH 6 antigen retrieval. Antibody at 1:250 dilution and detected with an Alexa Fluor 546 labeled goat-anti-rabbit ...read more

Immunofluorescence of human RCC tumor cryosections using NB100-417 (Panel A). Panel B shows staining with normal rabbit serum.

Colorectal cancer xenograft growth was suppressed by CB-PIC(20 and 50 mg/kg body weight) in female athymic nude mice. Starting three days after SW620 cell inoculation, CB-PIC (20 and 50 mg/kg body weight) was injected ...read more

Genetic Strategies: Iron depletion and nitric oxide stress and Iron depletion induce the truncated VDAC1 form accumulation. (D) Total protein extracts were analyzed by immunoblotting using VDACs poly antibody and ...read more

Immunohistochemical representative microphotographs representing the HIF-1 alpha , GLUT-1, and CAIX expression in endometrial cancer according to FIGO classification (IA, IB, II, IIIA, IIIC, and IV). Primary objective ...read more

The images are representative of immunohistochemistry staining of nuclear staining of proliferation marker Ki67, membrane protein CD 44, and CA IX. The images of scaffold culture (left panel) are compared with cell ...read more

Chemosensitivity of the CTC SCLC lines (IC50, mean ± SD) and immunohistochemistry of sections of tumorospheres. All differences between single cells (SC) and tumorospheres (TS) are statistically significant. ...read more

Rhebl1 re-introduction restored mitochondrial biogenesis in muscle-specific TAZ knockout mice.a RNA was isolated from gastrocnemius muscle of wild type (WT) and muscle-specific TAZ knockout (mKO) mice intramuscularly ...read more

Product Details

Summary

Reactivity	Hu, Mu, Rt, PlSpecies Glossary
Applications	WB, Simple Western, ELISA, Flow, Func, GS, ICC/IF, IHC, IP, MA, WB, ChIP, Dual ISH-IHC
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Format	BSA Free
Concentration	1.0 mg/ml

Validated by:

Biological Strategies

Genetic Strategies

Orthogonal Strategies

Order Details

View Available Conjugates

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB100-417AF350

Alexa Fluor 405 NB100-417AF405

Alexa Fluor 488 NB100-417AF488

Alexa Fluor 532 NB100-417AF532

Alexa Fluor 594 NB100-417AF594

Alexa Fluor 647 NB100-417AF647

Alexa Fluor 700 NB100-417AF700

Alexa Fluor 750 NB100-417AF750

Allophycocyanin NB100-417APC

Allophycocyanin/Cy7 NB100-417APCCY7

Biotin NB100-417B

DyLight 350 NB100-417UV

DyLight 405 NB100-417V

DyLight 488 NB100-417G

DyLight 550 NB100-417R

DyLight 594 NB100-417DL594

DyLight 650 NB100-417C

DyLight 680 NB100-417FR

DyLight 755 NB100-417IR

FITC NB100-417F

HRP NB100-417H

Janelia Fluor 549 NB100-417JF549

Janelia Fluor 646 NB100-417JF646

PE NB100-417PE

PE/Atto594 NB100-417PEATT594

PE/Cy5.5 NB100-417PECY55

PE/Cy7 NB100-417PECY7

PerCP NB100-417PCP

mFluor Violet 450 SE NB100-417MFV450

mFluor Violet 500 SE NB100-417MFV500

mFluor Violet 610 SE NB100-417MFV610

View Available Formulations

Catalog# & Formulation	Size	Price
BSA Free NB100-417SS

100% Guarantee on every product

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-417AF350
Alexa Fluor 405	NB100-417AF405
Alexa Fluor 488	NB100-417AF488
Alexa Fluor 532	NB100-417AF532
Alexa Fluor 594	NB100-417AF594
Alexa Fluor 647	NB100-417AF647
Alexa Fluor 700	NB100-417AF700
Alexa Fluor 750	NB100-417AF750
Allophycocyanin	NB100-417APC
Allophycocyanin/Cy7	NB100-417APCCY7
Biotin	NB100-417B
DyLight 350	NB100-417UV
DyLight 405	NB100-417V
DyLight 488	NB100-417G
DyLight 550	NB100-417R
DyLight 594	NB100-417DL594
DyLight 650	NB100-417C
DyLight 680	NB100-417FR
DyLight 755	NB100-417IR
FITC	NB100-417F
HRP	NB100-417H
Janelia Fluor 549	NB100-417JF549
Janelia Fluor 646	NB100-417JF646
PE	NB100-417PE
PE/Atto594	NB100-417PEATT594
PE/Cy5.5	NB100-417PECY55
PE/Cy7	NB100-417PECY7
PerCP	NB100-417PCP
mFluor Violet 450 SE	NB100-417MFV450
mFluor Violet 500 SE	NB100-417MFV500
mFluor Violet 610 SE	NB100-417MFV610

Carbonic Anhydrase IX/CA9 Antibody - BSA Free Summary

Immunogen	This Carbonic Anhydrase IX/CA9 Antibody was made from a synthetic peptide from the C-terminal sequence of human Carbonic Anhydrase IX (within residues 400-459) [UniProt# Q16790].
Localization	Membrane.
Marker	Hypoxia Marker
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	CA9
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

Chromatin Immunoprecipitation (ChIP) 1:10-1:500
Dual RNAscope ISH-IHC 1:1000
ELISA reported in scientific literature (PMID 19963243)
Flow Cytometry 1:1000
Gel Super Shift Assays
Immunoblotting
Immunocytochemistry/ Immunofluorescence 2 - 5 ug/ml
Immunohistochemistry 1:200 - 1:500
Immunohistochemistry-Frozen 1:200 - 1:500
Immunohistochemistry-Paraffin 1:200 - 1:500
Immunoprecipitation 1:10 - 1:500. Use reported in multiple pieces of scientific literature
Microarray reported in scientific literature (PMID 31955345)
Proximity Ligation Assay
Simple Western 1:50
Western Blot 1 - 3 ug/ml

Application Notes

In Western blot a band is observed approx. 53 kDa. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Control

HeLa Whole Cell Lysate
Carbonic Anhydrase IX/CA9 Overexpression Lysate

Control Peptide

Carbonic Anhydrase IX/CA9 Protein (NB100-417PEP)

Reviewed Applications

Read 7 Reviews rated 4.4

using
NB100-417 in the following applications:

Western Blot
Immunohistochemistry-Paraffin
Other
Immunocytochemistry

Publications

Read Publications using
NB100-417 in the following applications:

B/N
1 publication
Chemotaxis
2 publications
ELISA
1 publication
EMSA
1 publication
ICC/IF
18 publications
IF
1 publication
IF/ICC
1 publication
IF/IHC
76 publications
IHC
8 publications
IHC-Fr
12 publications
IHC-P
73 publications
MiAr
1 publication
PLA
1 publication
WB
43 publications

Packaging, Storage & Formulations

Storage	Store at -20C. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.02% Sodium Azide
Concentration	1.0 mg/ml
Purity	Immunogen affinity purified

Alternate Names for Carbonic Anhydrase IX/CA9 Antibody - BSA Free

CA9
CAIX
CA-IX
Carbonate dehydratase IX
carbonic anhydrase 9
Carbonic Anhydrase IX
carbonic dehydratase
EC 4.2.1.1
G250
Membrane antigen MN
MN
P54/58N
PMW1
RCC
RCC-associated antigen G250
RCC-associated protein G250
Renal cell carcinoma-associated antigen G250

Background

Carbonic anhydrase alpha, isozyme IX, belongs to a family of zinc-containing metalloproteins which hydrate carbon dioxide to generate bicarbonate ions and protons (1). This main catalytic function allows carbonic anhydrase IX to participate in cellular pH regulation. The large family of carbonic anhydrase metalloproteins includes three major classes which have been identified based on sequence and structure analysis. The alpha class is a monomer found in mammals. The beta class may occur as a dimer, tetramer, hexamer or octamer and is found in plants, algae, and bacteria. Lastly, the gamma class is a trimer found in bacteria and represents the most ancient carbonic anhydrase. These three classes of carbonic anhydrase enzymes lack sequence or structural similarities, but all share a conserved active site zinc atom (1).

Carbonic anhydrase IX (theoretical molecular weight 50kDa) belongs to the monomeric alpha class and is a single pass-transmembrane protein with two extracellular domains which serve catalytic and cell adhesion functions (2, 3). By cooperating with sodium bicarbonate cotransporters (NBC), lactate and proton exporting monocarboxylic acid transporters (MCT), and a sodium/hydrogen exchanger (NHE), carbonic anhydrase IX is involved in pH regulation across the cell membrane. This functional property protects cancer cells from intracellular acidification and partly explains the role of carbonic anhydrase IX in cancer cell survival and proliferation. In contrast, the pH regulating activity of carbonic anhydrase IX induces extracellular acidification, which has been implicated in epithelial to mesenchymal transition (EMT) and promoting cancer invasion. Carbonic anhydrase IX is frequently overexpressed in cancer cells (e.g., colorectal-, breast-, lung-carcinoma and brain tumors), an effect promoted by hypoxia within the tumor microenvironment (4). An exception are tumors carrying pVHL inactivating mutations, such as clear cell renal cell carcinoma (ccRCC), where HIF-alpha is stabilized due to dysfunctional proteasomal targeting and can induce HRE (Hypoxia Response Element) containing genes even under physiological normoxia (5). Carbonic anhydrase IX may be detected by immunostaining in tumors, which is found in association with necrotic tissue and metastatic cells. Because the expression of carbonic anhydrase IX correlates with both tumor grade and stage, analysis of its expression in tumors serves as a prognostic factor (4, 6).

References

1. Tripp, B. C., Smith, K., & Ferry, J. G. (2001). Carbonic Anhydrase: New Insights for an Ancient Enzyme. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.R100045200

2. Nishimori, I., & Onishi, S. (2001). Carbonic anhydrase isozymes in the human pancreas. Digestive and Liver Disease. https://doi.org/10.1016/s1590-8658(01)80138-9

3. Zavadova, Z., & Zavada, J. (2005). Carbonic anhydrase IX (CA IX) mediates tumor cell interactions with microenvironment. Oncology Reports. https://doi.org/10.3892/or.13.5.977

4. Pastorekova, S., & Gillies, R. J. (2019). The role of carbonic anhydrase IX in cancer development: links to hypoxia, acidosis, and beyond. Cancer and Metastasis Reviews. https://doi.org/10.1007/s10555-019-09799-0

5. Haase, V. (2009). The VHL Tumor Suppressor: Master Regulator of HIF. Current Pharmaceutical Design. https://doi.org/10.2174/138161209789649394

6. Young, J. R., Coy, H., Kim, H. J., Douek, M., Sisk, A., Pantuck, A. J., & Raman, S. S. (2018). Association of the gross appearance of intratumoral vascularity at MDCT with the carbonic anhydrase IX score in clear cell renal cell carcinoma. American Journal of Roentgenology. https://doi.org/10.2214/AJR.18.19725

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Carbonic Anhydrase IX/CA9 Antibody (NB100-417)(271)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Plant.

We have publications tested in 14 applications: B/N, Chemotaxis, ELISA, EMSA, ICC/IF, IF, IF/ICC, IF/IHC, IHC, IHC-Fr, IHC-P, MiAr, PLA, WB.

Showing Publications 1 - 10 of 271. Show All 271 Publications.

Publications using NB100-417	Applications	Species
Imanishi M, Inoue T, Fukushima K et al. CA9 and PRELID2; hypoxia-responsive potential therapeutic targets for pancreatic ductal adenocarcinoma as per bioinformatics analyses Journal of Pharmacological Sciences 2023-12-01 [PMID: 37973221] (WB, Human)	WB	Human
Pearsall SM, Williamson SC, Humphrey S et al. Lineage plasticity in SCLC generates non-neuroendocrine cells primed for vasculogenic mimicry Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 2023-07-14 [PMID: 37455012] (WB) Details: Dilutions: 1:500	WB
Zwergel C, Aventaggiato M, Garbo S et al. Novel 1,4-Dihydropyridines as Specific Binders and Activators of SIRT3 Impair Cell Viability and Clonogenicity and Downregulate Hypoxia-Induced Targets in Cancer Cells Journal of Medicinal Chemistry 2023-07-27 [PMID: 37439550] (WB)	WB
Wu J, Jing X, Du Q et al. Disruption of the Clock Component Bmal1 in Mice Promotes Cancer Metastasis through the PAI-1-TGF-?-myoCAF-Dependent Mechanism Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023-06-17 [PMID: 37330661] (IHC-P, Mouse) Details: IHC-P: antibdy dilution: 1:200. Antigen retrieval: citrate-based solution pH 6.0 (H-3300, Vector)	IHC-P	Mouse
Ito R, Yashiro M, Tsukioka T et al. Usefulness of pyruvate dehydrogenase-E1? expression to determine SUVmax cut-off value of [(18)F]FDG-PET for predicting lymph node metastasis in lung cancer Scientific Reports 2023-01-28 [PMID: 36709375] (IHC-P)	IHC-P
Perera RKS Investigating the role of the tumor microenvironment on tumor cell invasion and lymphatic dissemination Thesis (IF/IHC, Mouse)	IF/IHC	Mouse
Griesinger AM, Riemondy K, Eswaran N et al. Multi-omic approach identifies hypoxic tumor-associated myeloid cells that drive immunobiology of high-risk pediatric ependymoma iScience 2023-09-15 [PMID: 37694144]
Aboouf MA, Armbruster J, Guscetti F et al. Endogenous myoglobin expression in mouse models of mammary carcinoma reduces hypoxia and metastasis in PyMT mice Scientific reports 2023-05-09 [PMID: 37161046] (IHC, Mouse)	IHC	Mouse
Glassman D, Kim MS, Spradlin M et al. Exploiting metabolic vulnerabilities after anti-VEGF antibody therapy in ovarian cancer iScience 2023-02-17 [PMID: 36824283]
Eloranta K, Pihlajoki M, Liljeström E et al. SLC-0111, an inhibitor of carbonic anhydrase IX, attenuates hepatoblastoma cell viability and migration Frontiers in oncology 2023-01-26 [PMID: 36776327] (WB)	WB
Mardente S, Aventaggiato M, Splendiani E et al. Extra-Cellular Vesicles Derived from Thyroid Cancer Cells Promote the Epithelial to Mesenchymal Transition (EMT) and the Transfer of Malignant Phenotypes through Immune Mediated Mechanisms International journal of molecular sciences 2023-02-01 [PMID: 36769076] (WB, Human)	WB	Human
Magnusson P, Jauhiainen S, Onyeogaziri F et al. Proteomics on Human Cerebral Cavernous Malformations Reveals Novel Biomarkers for the Disease Pathology SSRN 2023-11-06
Fujita N, Bondoc A, Ishida J et al. Combination Treatment with Histone Deacetylase and Carbonic Anhydrase 9 Inhibitors Shows Therapeutic Potential in Experimental Diffuse Intrinsic Pontine Glioma Research Square 2022-03-10 (WB, Human)	WB	Human
Massenet-Regad L, Poirot J, Jackson M et al. Large-scale analysis of cell-cell communication reveals angiogenin-dependent tumor progression in clear cell renal cell carcinoma iScience 2023-10-01 [PMID: 38025776] (IF/ICC, Human)	IF/ICC	Human
Fantin J, Toutain J, Pérès EA et al. Assessment of hypoxia and oxidative-related changes in a lung-derived brain metastasis model by [64Cu][Cu(ATSM)] PET and proteomic studies EJNMMI research 2023-11-25 [PMID: 38006431] (IHC-Fr, Human)	IHC-Fr	Human
Sun X, Sui W, Mu Z et al. Mirabegron displays anticancer effects by globally browning adipose tissues Nature communications 2023-11-22 [PMID: 37993438] (IHC, Mouse)	IHC	Mouse
Rao H, Liu C, Wang A et al. SETD2 deficiency accelerates sphingomyelin accumulation and promotes the development of renal cancer Nature communications 2023-11-21 [PMID: 37989747] (IHC, Mouse)	IHC	Mouse
Vinciguerra A, Bedarida V, Pronier C et al. Expression, Prognostic Value and Correlation with HPV Status of Hypoxia-Induced Markers in Sinonasal Squamous Cell Carcinoma Journal of Personalized Medicine 2023-04-29 [PMID: 37240937] (IHC-P, Human)	IHC-P	Human
Aventaggiato M, Preziosi A, Cheraghi Bidsorkhi H et al. ZnO Nanorods Create a Hypoxic State with Induction of HIF-1 and EPAS1, Autophagy, and Mitophagy in Cancer and Non-Cancer Cells International journal of molecular sciences 2023-04-09 [PMID: 37108134] (WB, Human)	WB	Human
Aubin RG, Montelongo J, Hu R, Camara PG Clustering-independent estimation of cell abundances in bulk tissues using single-cell RNA-seq data bioRxiv : the preprint server for biology 2023-02-07 [PMID: 36798206] (IHC-P, Human)	IHC-P	Human
Li Y, Lih TM, Dhanasekaran SM et al. Histopathologic and proteogenomic heterogeneity reveals features of clear cell renal cell carcinoma aggressiveness Cancer cell 2022-12-20 [PMID: 36563681] (IHC-P, Human)	IHC-P	Human
Jing X, Wu J, Dong C et al. COVID-19 instigates adipose browning and atrophy through VEGF in small mammals Nature metabolism 2022-12-01 [PMID: 36482111] (IHC-P, WB, Mouse)	IHC-P, WB	Mouse
Agudelo JP, Upadhyay D, Zhang D et al. Multiparametric Magnetic Resonance Imaging and Metabolic Characterization of Patient-Derived Xenograft Models of Clear Cell Renal Cell Carcinoma Metabolites 2022-11-15 [PMID: 36422257] (IHC-P, Human)	IHC-P	Human
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Kim En, Kim Gh, Kim J et al. A Pyloric Gland-Phenotype Ovarian Mucinous Tumor Resembling Lobular Endocervical Glandular Hyperplasia in a Patient with Peutz-Jeghers Syndrome. J Pathol Transl Med 2016-08-22 [PMID: 27550049] (IHC-P)	IHC-P
van Kuijk SJ, Gieling RG, Niemans R et al. The Sulfamate Small Molecule CAIX Inhibitor S4 Modulates Doxorubicin Efficacy. PLoS ONE. 2016-08-12 [PMID: 27513947] (IF/IHC, Human)	IF/IHC	Human
Hirakawa T, Yashiro M, Doi Y et al. Pancreatic Fibroblasts Stimulate the Motility of Pancreatic Cancer Cells through IGF1/IGF1R Signaling under Hypoxia. PLoS ONE. 2016-08-04 [PMID: 27487118] (IF/IHC, Human)	IF/IHC	Human
Yoon C, Chang KK, Lee JH et al. Multimodal targeting of tumor vasculature and cancer stem-like cells in sarcomas with VEGF-A inhibition, HIF-1a inhibition, and hypoxia-activated chemotherapy Oncotarget 2016-07-12 [PMID: 27374091] (Mouse)		Mouse
Leek R, Grimes DR, Harris AL, McIntyre A. Methods: Using Three-Dimensional Culture (Spheroids) as an In Vitro Model of Tumour Hypoxia. Adv. Exp. Med. Biol. 2016-06-21 [PMID: 27325267] (IF/IHC, Mouse, Human)	IF/IHC	Mouse, Human
He H, Yang Y, Xiang Z et al. A Sensitive IHC Method for Monitoring Autophagy-Specific Markers in Human Tumor Xenografts. J Biomark. 2016-06-01 [PMID: 27247826] (IHC-P, Human)	IHC-P	Human
Hecht JL, Zsengeller ZK, Spiel M, Karumanchi SA. Revisiting decidual vasculopathy. Placenta. 2016-04-08 [PMID: 27238712] (IHC-P, Human)	IHC-P	Human
Xu W, Ghosh S, Comhair SA et al. Increased mitochondrial arginine metabolism supports bioenergetics in asthma. J. Clin. Invest. 2016-05-23 [PMID: 27214549] (IHC-P, Mouse)	IHC-P	Mouse
Badar T, Handisides DR, Benito JM et al. A Phase I Study of Evofosfamide, an Investigational Hypoxia-Activated Prodrug, in Patients with Advanced Leukemia. Am. J. Hematol. 2016-05-11 [PMID: 27169385] (IHC-P, Human)	IHC-P	Human
Faes S, Planche A, Uldry E et al. Targeting carbonic anhydrase IX improves the anti-cancer efficacy of mTOR inhibitors. Oncotarget. 2016-06-14 [PMID: 27153561] (IF/IHC, Mouse)	IF/IHC	Mouse
Ono M, Yamada K, Bensaddek D et al. Enhanced snoMEN Vectors Facilitate Establishment of GFP-HIF-1alpha Protein Replacement Human Cell Lines. PLoS ONE. 2016-04-30 [PMID: 27128805] (WB, Human)	WB	Human
Eikrem O, Beisland C, Hjelle K et al. Transcriptome Sequencing (RNAseq) Enables Utilization of Formalin-Fixed, Paraffin-Embedded Biopsies with Clear Cell Renal Cell Carcinoma for Exploration of Disease Biology and Biomarker Development PLoS ONE 2016-02-23 [PMID: 26901863] (IHC-P, Human)	IHC-P	Human
Radoul M, Chaumeil MM, Eriksson P et al. MR Studies of Glioblastoma Models Treated with Dual PI3K/mTOR Inhibitor and Temozolomide:Metabolic Changes Are Associated with Enhanced Survival. Mol. Cancer Ther. 2016-05-01 [PMID: 26883274] (IF/IHC, Mouse)	IF/IHC	Mouse
Scholz A, Harter PN, Cremer S et al. Endothelial cell-derived angiopoietin-2 is a therapeutic target in treatment-naive and bevacizumab-resistant glioblastoma. EMBO Mol Med. 2016-01-05 [PMID: 26666269] (IHC-P, Human)	IHC-P	Human
Ortmann B, Bensaddek D, Carvalhal S et al. CDK-dependent phosphorylation of PHD1 on serine 130 alters its substrate preference in cells J Cell Sci. 2015-12-31 [PMID: 26644182] (WB, Human)	WB	Human
Mitsuhashi A, Goto H, Saijo A et al. Fibrocyte-like cells mediate acquired resistance to anti-angiogenic therapy with bevacizumab. Nat Commun. 2015-12-04 [PMID: 26635184] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Andre H, Tunik S, Aronsson M, Kvanta A. Hypoxia-Inducible Factor-1alpha Is Associated With Sprouting Angiogenesis in the Murine Laser-Induced Choroidal Neovascularization Model. ARVO Journals. 2015-10-01 [PMID: 26501235] (ICC/IF, Mouse)	ICC/IF	Mouse
Klier M, Jamali S, Ames S et al. Catalytic activity of human carbonic anhydrase isoform IX is displayed both extra- and intracellularly. FEBS J 2016-01-01 [PMID: 26470855]
Islam SS, Mokhtari RB, Akbari P et al. Simultaneous Targeting of Bladder Tumor Growth, Survival, and Epithelial-to-Mesenchymal Transition with a Novel Therapeutic Combination of Acetazolamide (AZ) and Sulforaphane (SFN). Target Oncol 2015-10-10 [PMID: 26453055]
Kinoshita H, Yashiro M, Fukuoka T et al. Diffuse-type Gastric Cancer Cells Switch Their Driver Pathways from FGFR2 Signaling to SDF1/CXCR4 Axis in Hypoxic Tumor Microenvironments. Carcinogenesis 2015-09-18 [PMID: 26385890] (IHC-P, Mouse)	IHC-P	Mouse
Jamali S, Klier M, Ames S et al. Hypoxia-induced carbonic anhydrase IX facilitates lactate flux in human breast cancer cells by non-catalytic function. Sci Rep 2015-09-04 [PMID: 26337752] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Baine MK, Turcu G, Zito CR et al. Characterization of tumor infiltrating lymphocytes in paired primary and metastatic renal cell carcinoma specimens. Oncotarget 2015-09-22 [PMID: 26317902]
Song CW, Lee YJ, Griffin RJ et al. Indirect Tumor Cell Death After High-Dose Hypofractionated Irradiation: Implications for Stereotactic Body Radiation Therapy and Stereotactic Radiation Surgery. Int J Radiat Oncol Biol Phys 2015-09-01 [PMID: 26279032]
Ward C, Meehan J, Mullen P et al. Evaluation of carbonic anhydrase IX as a therapeutic target for inhibition of breast cancer invasion and metastasis using a series of in vitro breast cancer models Oncotarget 2015-07-04 [PMID: 26259239]
Kasashima H, Yashiro M, Kinoshita H et al. Lysyl oxidase is associated with the epithelial-mesenchymal transition of gastric cancer cells in hypoxia. Gastric Cancer 2016-04-01 [PMID: 26100130]
Yoon C, Lee HJ, Park DJ et al. Hypoxia-activated chemotherapeutic TH-302 enhances the effects of VEGF-A inhibition and radiation on sarcomas. Br. J. Cancer. 2015-05-26 [PMID: 26010414] (ICC/IF, IHC-P, Human)	ICC/IF, IHC-P	Human
Brossa Alessia, Grange Cristina, Mancuso Letizia et al. Sunitinib but not VEGF blockade inhibits cancer stem cell endothelial differentiation. Oncotarget 2015-01-01 [PMID: 25948774] (IF/IHC, ICC/IF, Mouse)	IF/IHC, ICC/IF	Mouse
Dulloo I, Phang BH, Othman R et al. Hypoxia-inducible TAp73 supports tumorigenesis by regulating the angiogenic transcriptome. Nat. Cell Biol. 2015-04-01 [PMID: 25774835] (IHC-P, Human)	IHC-P	Human
Cotroneo E, Ashek A, Wang L et al. Iron Homeostasis and Pulmonary Hypertension: Iron Deficiency Leads to Pulmonary Vascular Remodeling in the Rat. Circ. Res. 2015-03-12 [PMID: 25767292] (IF/IHC, Rat)	IF/IHC	Rat
Kang HJ, Kim IH, Sung CO et al. Expression of carbonic anhydrase 9 is a novel prognostic marker in resectable hepatocellular carcinoma Virchows Arch. 2015-01-11 [PMID: 25577552] (IHC-P, Human) Details: Carbonic Anhydrase IX/CA9 antibody used at 1:1000 dilution for IHC-P staining on formalin-fixed, paraffin-embedded tissue sections/TMAs created from surgically resected hepatocellular carcinoma/HCC and adjacent non-neoplastic liver tissue - 32 minutes of heat-induced epitope retrieval with Cell Conditioning 1 /CC1 buffer, 16 minutes of primary antibody incubation on Ventana's autoimmunostainer/Bench Mark XT automatic immunostaining device, staining development with Ventana's OptiView DAB IHC Detection Kit. Fig 2a-f documents the staining images showing weak, moderate and strong membranous expression levels of carbonic anhydrase 9 protein and the overall data in this study establishes CA9 protein as a poor prognostic factor in resectable HCC patients.	IHC-P	Human
Mudie S, Bandarra D, Batie M et al. PITX1, a specificity determinant in the HIF-1alpha-mediated transcriptional response to hypoxia. Cell Cycle 2014-01-01 [PMID: 25558831] (WB)	WB
Lee H, Yoon C, Park DJ et al. Inhibition of Vascular Endothelial Growth Factor A and Hypoxia-Inducible Factor 1alpha Maximizes the Effects of Radiation in Sarcoma Mouse Models Through Destruction of Tumor Vasculature. Int. J. Radiat. Oncol. Biol. Phys. 2014-12-24 [PMID: 25544668]
Janmohamed SR, Brinkhuizen T, den Hollander JC et al. Support for the hypoxia theory in the pathogenesis of infantile haemangioma. Clin. Exp. Dermatol. 2014-12-16 [PMID: 25511669] (IHC-P, Human)	IHC-P	Human
Lin XY, Zhang H, Tang N et al. Expression and diagnostic implications of carbonic anhydrase IX in several tumours with predominantly clear cell morphology. Histopathology. 2014-11-27 [PMID: 25431204] (IHC-P, Human) Details: Carbonic Anhydrase IX/CA9 antibody used for IHC-P on sections of clear cell renal cell carcinoma (ccRCC; primary and metastatic) and several other type of tumors with predominantly clear cell morphology - tissues previously fixed in 10% neutral buffered formalin and embedded in paraffin, 4um sections, routine IHC-P processing with streptavidin-peroxidase detection system. ccRCC, and breast / pulmonary/hepatic clear cell carcinoma, urothelial carcinoma with clear cell change, clear cell meningioma/ependymoma, haemangioblastoma, and clear cell hidradenoma showed expression of CAIX. See full text for staining images and the expression levels.	IHC-P	Human
Gellert LL, Mehra R, Chen YB et al. The diagnostic accuracy of percutaneous renal needle core biopsy and its potential impact on the clinical management of renal cortical neoplasms. Arch. Pathol. Lab. Med. 2014-12-01 [PMID: 25427045] (IHC-P, Human) Details: Carbonic Anhydrase IX/CA9 antibody used for IHC-P on sections of human renal biopsies having sarcomatoid area with focal necrosis and clear cell renal cell carcinoma (Figure 3.).	IHC-P	Human
Santos Cd, Tijeras-Raballand A, Serova M et al. Effects of preset sequential administrations of sunitinib and everolimus on tumour differentiation in Caki-1 renal cell carcinoma. Br. J. Cancer. 2014-11-25 [PMID: 25422908] (IHC-P, Human)	IHC-P	Human
Meijer Tineke W H, Bussink Johan, Zatovicova Miriam et al. Tumor microenvironmental changes induced by the sulfamate carbonic anhydrase IX inhibitor S4 in a laryngeal tumor model. PloS One 2014-01-01 [PMID: 25225880] (IF/IHC, Mouse)	IF/IHC	Mouse
Hwa JS, Kwon OJ, Park JJ et al. The prognostic value of immunohistochemical markers for oral tongue squamous cell carcinoma. Eur Arch Otorhinolaryngol. 2014-08-29 [PMID: 25169079]
Chen LQ, Howison CM, Spier C et al. Assessment of carbonic anhydrase IX expression and extracellular pH in B-cell lymphoma cell line models. Leuk. Lymphoma. 2014-08-17 [PMID: 25130478]
Motzer Robert J, Hutson Thomas E, Hudes Gary R et al. Investigation of novel circulating proteins, germ line single-nucleotide polymorphisms, and molecular tumor markers as potential efficacy biomarkers of first-line sunitinib therapy for advanced renal cell carcinoma. Cancer Chemother Pharmacol. 2014-08-07 [PMID: 25100134] (IHC-P, Human)	IHC-P	Human
Guo Jianying, Shinriki Satoru, Su Yu et al. Hypoxia suppresses cylindromatosis (CYLD) expression to promote inflammation in glioblastoma: possible link to acquired resistance to anti-VEGF therapy. Oncotarget 2014-01-01 [PMID: 25071012] (IF/IHC, Human)	IF/IHC	Human
Sadlecki P, Bodnar M, Grabiec M. The Role of Hypoxia-Inducible Factor-1a, Glucose Transporter-1,(GLUT-1) and Carbon Anhydrase IX in Endometrial Cancer Patients. BioMed Research International 2014-03-12 [PMID: 24745019] (IHC-P, Human)	IHC-P	Human
Heijmen L, Voert EGW, Punt CJA. Monitoring hypoxia and vasculature during bevacizumab treatment in a murine colorectal cancer model. Contrast Media Mol Imaging 2014-05-01 [PMID: 24700751] (IHC-Fr, Human) Details: Using the Biotin conjugated version of NB100-417, catalog number NB100-417B.	IHC-Fr	Human
Kwon OJ, Park JJ, Ko GH et al. HIF-1a and CA-IX as predictors of locoregional control for determining the optimal treatment modality for early-stage laryngeal carcinoma. Head Neck 2014-02-12 [PMID: 24677746] (IHC-P, Human)	IHC-P	Human
Chen X, Iliopoulos D, Zhang Q et al. XBP1 promotes triple-negative breast cancer by controlling the HIF1a pathway. Nature 2014-04-03 [PMID: 24670641] (IHC-P, Human)	IHC-P	Human
Pinato DJ, Tan TM, Toussi ST et al. An expression signature of the angiogenic response in gastrointestinal neuroendocrine tumours: correlation with tumour phenotype and survival outcomes. Br J Cancer. 2013-11-14 [PMID: 24231952] (IHC-P, Human)	IHC-P	Human
Wen YA, Stevens PD, Gasser ML et al. Downregulation of PHLPP Expression Contributes to Hypoxia-Induced Resistance to Chemotherapy in Colon Cancer Cells. Mol Cell Biol. 2013-11-01 [PMID: 24061475] (WB, Human)	WB	Human
Wasser, Y, Y The Role of Dlk1 in the Gene Regulatory Network Underlying Motor Neuron Diversification Thesis 2022-01-01 (WB, Mouse)	WB	Mouse
Morifuji Yoshihiro, Onishi Hideya, Iwasaki Hironori et al. Reoxygenation from chronic hypoxia promotes metastatic processes in pancreatic cancer through the Hedgehog signaling. Cancer Sci. 2014-02-11 [PMID: 24397700]
Polanski Radoslaw, Hodgkinson Cassandra L, Fusi Alberto et al. Activity of the monocarboxylate transporter 1 inhibitor AZD3965 in small cell lung cancer. Clin Cancer Res. 2014-02-15 [PMID: 24277449] (IF/IHC)	IF/IHC
Chaudary N, Pintilie M, Schwock J et al. Characterization of the Tumor-Microenvironment in Patient-Derived Cervix Xenografts (OCICx). Cancers (Basel) 2012-01-01 [PMID: 24213469] (IF/IHC)	IF/IHC
Zhang M, Boughton P, Rose B et al. The Use of Porous Scaffold as a Tumor Model. Int J Biomater. 2013-01-01 [PMID: 24101930] (IF/IHC, Human)	IF/IHC	Human
El-Heliebi A, Kroneis T, Zohrer E et al. Are morphological criteria sufficient for the identification of circulating tumor cells in renal cancer?. J Transl Med. 2013-09-17 [PMID: 24044779] (ICC/IF, Human)	ICC/IF	Human
Fahling M, Mathia S, Paliege A et al. Tubular von Hippel-Lindau Knockout Protects against Rhabdomyolysis-Induced AKI. J Am Soc Nephrol. 2013-08-22 [PMID: 23970125]
Cioffi S, Martucciello S, Fulcoli FG et al. Tbx1 regulates brain vascularization. Hum Mol Genet. 2013-09-07 [PMID: 23945394] (ICC/IF, IHC-Fr, Mouse)	ICC/IF, IHC-Fr	Mouse
Kumar S, Mokhtari RB, Oliveira ID et al. Tumor Dynamics in Response to Antiangiogenic Therapy with Oral Metronomic Topotecan and Pazopanib in Neuroblastoma Xenografts. Transl Oncol 2013-08-01 [PMID: 23908692] (IF/IHC, Human)	IF/IHC	Human
Dubois L, Peeters SG, van Kuijk SJ et al. Targeting carbonic anhydrase IX by nitroimidazole based sulfamides enhances the therapeutic effect of tumor irradiation: A new concept of dual targeting drugs. Radiother Oncol 2013-07-09 [PMID: 23849171] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Campanile C, Arlt MJ, Kramer SD et al. Characterization of Different Osteosarcoma Phenotypes by PET Imaging in Preclinical Animal Models. J Nucl Med 2013-06-25 [PMID: 23801674]
Bussolati B, Lauritano C, Moggio A et al. Renal CD133+/CD73+ Progenitors Produce Erythropoietin under Hypoxia and Prolyl Hydroxylase Inhibition. J Am Soc Nephrol 2013-05-09 [PMID: 23661806]
Carbonell WS, Delay M, Jahangiri A et al. beta1 Integrin Targeting Potentiates Antiangiogenic Therapy and Inhibits the Growth of Bevacizumab-Resistant Glioblastoma. Cancer Res 2013-05-15 [PMID: 23644530] (IHC-P, ICC/IF, Human)	IHC-P, ICC/IF	Human
Cho SY, Lee HJ, Lee HJ et al. Activation of AMP-Activated Protein Kinase alpha and Extracelluar Signal-Regulated Kinase Mediates CB-PIC-Induced Apoptosis in Hypoxic SW620 Colorectal Cancer Cells Evid Based Complement Alternat Med 2013-01-01 [PMID: 23589723] (IHC-P, Human)	IHC-P	Human
Aarntzen EH, Srinivas M, Bonetto F et al. Targeting of 111In-Labeled Dendritic Cell Human Vaccines Improved by Reducing Number of Cells. Clin Cancer Res 2013-03-15 [PMID: 23382117] (IF/IHC, Human)	IF/IHC	Human
Zhang Q, Bai X, Chen W et al. Wnt/beta-catenin signaling enhances hypoxia-induced epithelial-mesenchymal transition in hepatocellular carcinoma via crosstalk with hif-1alpha signaling. Carcinogenesis 2013-01-27 [PMID: 23358852] (WB, Human)	WB	Human
Heijmen L, Punt CJ, Ter Voert EG et al. Monitoring the effects of bevacizumab beyond progression in a murine colorectal cancer model: a functional imaging approach. Invest New Drugs 2013-01-17 [PMID: 23325291] (ICC/IF, IF/IHC, Mouse) Details: Using the Biotin conjugated version of NB100-417, catalog number NB100-417B.	ICC/IF, IF/IHC	Mouse
Pinato DJ, Ramachandran R, Toussi ST et al. Immunohistochemical markers of the hypoxic response can identify malignancy in phaeochromocytomas and paragangliomas and optimize the detection of tumours with VHL germline mutations Br J Cancer 2012-12-20 [PMID: 23257898] (IF/IHC, Human)	IF/IHC	Human
Delhaas T, Van der Meer SF, Schaart G et al. Steep Increase in Myonuclear Domain Size During Infancy Anat Rec (Hoboken) 2012-12-04 [PMID: 23213045] (IF/IHC, ICC/IF, Human)	IF/IHC, ICC/IF	Human
Proescholdt MA, Merrill MJ, Stoerr EM et al. Function of carbonic anhydrase IX in glioblastoma multiforme Neuro Oncol 2012-11-01 [PMID: 23074198] (IF/IHC, WB, Human)	IF/IHC, WB	Human
Currie MJ, Beardsley BE, Harris GC et al. Immunohistochemical analysis of cancer stem cell markers in invasive breast carcinoma and associated ductal carcinoma in situ: relationships with markers of tumor hypoxia and microvascularity Hum Pathol 2012-10-01 [PMID: 23036368] (IF/IHC, Human)	IF/IHC	Human
Piao Y, Liang J, Holmes L et al. Glioblastoma resistance to anti-VEGF therapy is associated with myeloid cell infiltration, stem cell accumulation, and a mesenchymal phenotype Neuro Oncol 2012-09-10 [PMID: 22965162] (IF/IHC, Human, Mouse)	IF/IHC	Human, Mouse
Muriel Lopez C, Esteban E, Berros JP et al. Prognostic Factors in Patients With Advanced Renal Cell Carcinoma Clin Genitourin Cancer 2012-09-06 [PMID: 22959659] (IF/IHC, Human)	IF/IHC	Human
Brouillet S, Murthi P, Hoffmann P et al. EG-VEGF controls placental growth and survival in normal and pathological pregnancies: case of fetal growth restriction (FGR) Cell Mol Life Sci 2012-09-02 [PMID: 22941044] (IF/IHC, Human)	IF/IHC	Human
Engler JR, Robinson AE, Smirnov I et al. Increased microglia/macrophage gene expression in a subset of adult and pediatric astrocytomas PLoS One 2012-01-01 [PMID: 22937035] (IF/IHC, Human)	IF/IHC	Human
Yin S, Kaluz S, Devi NS et al. Arylsulfonamide KCN1 Inhibits In Vivo Glioma Growth and Interferes with HIF Signaling by Disrupting HIF-1alpha Interaction with Cofactors p300/CBP Clin Cancer Res 2012-12-15 [PMID: 22923450] (WB, Human)	WB	Human
Park I, Hu S, Bok R et al. Evaluation of heterogeneous metabolic profile in an orthotopic Human glioblastoma xenograft model using compressed sensing hyperpolarized 3D 13C magnetic resonance spectroscopic imaging. Magn Reson Med 2013-07-01 [PMID: 22851374] (IHC-P, Rat)	IHC-P	Rat
Fromy B, Lingueglia E, Sigaudo-Roussel D et al. Asic3 is a neuronal mechanosensor for pressure-induced vasodilation that protects against pressure ulcers Nat Med 2012-07-29 [PMID: 22842475] (IF/IHC, Mouse)	IF/IHC	Mouse
Eguchi D, Ikenaga N, Ohuchida K et al. Hypoxia enhances the interaction between pancreatic stellate cells and cancer cells via increased secretion of connective tissue growth factor J Surg Res 2012-07-06 [PMID: 22795353] (IF/IHC, Human)	IF/IHC	Human
Albert S, Hourseau M, Halimi C et al. Prognostic value of the chemokine receptor CXCR4 and epithelial-to-mesenchymal transition in patients with squamous cell carcinoma of the mobile tongue. Oral Oncol 2012-12-01 [PMID: 22776129]
Muriel Lopez C, Esteban E, Astudillo A, Pardo P, Berros JP, Izquierdo M, Crespo G, Fonseca PJ, Sanmamed M, Martinez-Camblor P. Predictive factors for response to treatment in patients with advanced renal cell carcinoma. Invest New Drugs. 2012-05-27 [PMID: 22644070] (IF/IHC, Human)	IF/IHC	Human
Pellegrini L, Pucci B, Villanova L et al. SIRT3 protects from hypoxia and staurosporine-mediated cell death by maintaining mitochondrial membrane potential and intracellular pH Cell Death Differ [PMID: 22595756] (WB, Human) Details: Used the IHC-Prediluted form of this antibody.	WB	Human
Weber DC, Tille JC, Combescure C et al. The prognostic value of expression of HIF1alpha, EGFR and VEGF-A, in localized prostate cancer for intermediate- and high-risk patients treated with radiation therapy with or without androgen deprivation therapy Radiat Oncol 2012-04-30 [PMID: 22546016] (IF/IHC, Human)	IF/IHC	Human
Onishi H, Morifuji Y, Kai M, Suyama K, Iwasaki H, Katano M. Hedgehog inhibitor decreases chemosensitivity to 5-fluorouracil and gemcitabine under hypoxic conditions in pancreatic cancer. Cancer Sci. 2012-04-06 [PMID: 22486854] (IF/IHC, ICC/IF, Mouse)	IF/IHC, ICC/IF	Mouse
Speisky D, Duces A, Bieche I et al. Molecular Profiling of Pancreatic Neuroendocrine Tumors in Sporadic and Von Hippel-Lindau Patients. Clin Cancer Res;18(10):2838-2849. 2012-05-15 [PMID: 22461457] (IF/IHC, Human)	IF/IHC	Human
Matsumura Y, Ishii G, Aokage K, Kuwata T, Hishida T, Yoshida J, Nishimura M, Nagai K, Ochiai A. Morphophenotypic characteristics of intralymphatic cancer and stromal cells susceptible to lymphogenic metastasis. Cancer Sci. 2012-03-16 [PMID: 22429811] (IF/IHC, Human)	IF/IHC	Human
Wang Y, Roche O, Xu C et al. Hypoxia promotes ligand-independent EGF receptor signaling via hypoxia-inducible factor-mediated upregulation of caveolin-1 Proc Natl Acad Sci U S A 2012-03-12 [PMID: 22411794] (WB, EMSA, Human)	WB, EMSA	Human
Lim CS, Kiriakidis S, Paleolog EM, Davies AH. Increased activation of the hypoxia-inducible factor pathway in varicose veins. J Vasc Surg;55(5):1427-1439e1. 2012-05-01 [PMID: 22277691] (WB, Human)	WB	Human
Deschamps L, Bacha D, Rebours V, Mebarki M, Bretagnol F, Panis Y, Bedossa P, Ruszniewski P, Couvelard A. The Expression of the Hypoxia Markers CA9 and CXCR4 Is Correlated with Survival in Patients with Neuroendocrine Tumours of the Ileum. Neuroendocrinology. 2011-11-29 [PMID: 22133596] (IF/IHC, Human)	IF/IHC	Human
Jung JH, Kim HJ, Yeom J, Yoo C, Shin J, Yoo J, Kang CS, Lee C. Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer. J Gastroenterol;47(1):37-48. 2012-01-01 [PMID: 22015694] (WB, Human)	WB	Human
Guise CP, Abbattista MR, Tipparaju SR et al. Diflavin Oxidoreductases Activate the Bioreductive Prodrug PR-104A under Hypoxia. Mol Pharmacol. 2011-10-07 [PMID: 21984255]
Ogino T, Onishi H, Suzuki H et al. Inclusive estimation of complex antigen presentation functions of monocyte-derived dendritic cells differentiated under normoxia and hypoxia conditions. Cancer Immunology, Immunotherapy: CII. 2011-09-20 [PMID: 21932134]
Melvin A, Mudie S, Rocha S. The chromatin remodeler ISWI regulates the cellular response to hypoxia: role of FIH. Mol Biol Cell 2011-11-01 [PMID: 21900490] (Human)		Human
Benito J, Shi Y, Szymanska B et al. Pronounced Hypoxia in Models of Murine and Human Leukemia: High Efficacy of Hypoxia-Activated Prodrug PR-104. PLoS ONE. 2011-01-01 [PMID: 21853076]
Garcia-Parra R, Wood D, Shah RB et al. Investigation on tumor hypoxia in resectable primary prostate cancer as demonstrated by (18)F-FAZA PET/CT utilizing multimodality fusion techniques. Eur J Nucl Med Mol Imaging. 2011-07-16 [PMID: 21833840] (IHC-P, Human)	IHC-P	Human
Chaumeil MM, Ozawa T, Park I et al. Hyperpolarized 13C MR spectroscopic imaging can be used to monitor Everolimus treatment in vivo in an orthotopic rodent model of glioblastoma. NeuroImage. [PMID: 21807103] (IHC-P, Rat)	IHC-P	Rat
Chamboredon S, Ciais D, Desroches-Castan A et al. Hypoxia-inducible Factor-1{alpha} mRNA: a new target for destabilization by tristetraprolin in endothelial cells. Mol Biol Cell. 2011-07-20 [PMID: 21775632] (WB, Human)	WB	Human
Al-Ahmadie HA, Alden D, Fine SW et al. Role of immunohistochemistry in the evaluation of needle core biopsies in adult renal cortical tumors: an ex vivo study. Am. J. Surg. Pathol. 2011-07-01 [PMID: 21677535] (IF/IHC, Human)	IF/IHC	Human
Mackenzie KA, Miller AP, Hock BD, Gardner J, Simcock JW, Roake JA, Dachs GU, Robinson BA, Currie MJ. Angiogenesis and host immune response contribute to the aggressive character of non-melanoma skin cancers in renal transplant recipients. Histopathology;58(6):875-85. 2011-05-01 [PMID: 21585427] (IF/IHC, Human)	IF/IHC	Human
Nelson MP, Christmann BS, Werner JL et al. IL-33 and M2a alveolar macrophages promote lung defense against the atypical fungal pathogen Pneumocystis murina. J Immunol 2011-02-15 [PMID: 21220696] (Chemotaxis)	Chemotaxis
Dachs Gabi U, Kano Maiko, Volkova Ekaterina et al. A profile of prognostic and molecular factors in European and Maori breast cancer patients. BMC Cancer 2010-01-01 [PMID: 20932344] (IF/IHC, Human)	IF/IHC	Human
Li Y, Wang H, Tu C et al. Role of hypoxia and EGF on expression, activity, localization and phosphorylation of carbonic anhydrase IX in MDA-MB-231 breast cancer cells. Biochim Biophys Acta. 2010-10-01 [PMID: 20920536] (WB, Human)	WB	Human
Lapinski JE, Chen L, Zhou M. Distinguishing clear cell renal cell carcinoma, retroperitoneal paraganglioma, and adrenal cortical lesions on limited biopsy material: utility of immunohistochemical markers. Appl Immunohistochem Mol Morphol;18(5):414-21. 2010-10-01 [PMID: 20861762] (IF/IHC, Human)	IF/IHC	Human
O'Toole D, Couvelard A, Rebours V et al. Molecular markers associated with response to chemotherapy in gastro-entero-pancreatic neuroendocrine tumors. Endocr Relat Cancer 2010-12-01 [PMID: 20570957] (IF/IHC, Human)	IF/IHC	Human
DeJohn C Noninvasive Imaging of the Immune-Vascular Crosstalk in Head and Neck Cancer Thesis 2023-01-01
Newton IP, Kenneth NS, Appleton PL et al. Adenomatous polyposis coli and hypoxia-inducible factor-1{alpha} have an antagonistic connection. Mol Biol Cell 2010-11-01 [PMID: 20844082] (Chemotaxis)	Chemotaxis
Pistollato F, Rampazzo E, Persano L et al. Interaction of hypoxia-inducible factor-1a and Notch signaling regulates medulloblastoma precursor proliferation and fate. Stem Cells 2010-11-01 [PMID: 20827750] (IHC-P, Human)	IHC-P	Human
Kato Y, Yashiro M, Noda S et al. Expression of a hypoxia-associated protein, carbonic anhydrase-9, correlates with malignant phenotypes of gastric carcinoma. Digestion 2010-01-01 [PMID: 20588040] (IF/IHC, Human)	IF/IHC	Human
Stillebroer AB, Mulders PF, Boerman OC et al. Carbonic anhydrase IX in renal cell carcinoma: implications for prognosis, diagnosis, and therapy. Eur Urol 2010-07-01 [PMID: 20359812]
Pistollato F, Abbadi S, Rampazzo E et al. Intratumoral hypoxic gradient drives stem cells distribution and MGMT expression in glioblastoma. Stem Cells 2010-05-01 [PMID: 20309962] (IHC-P, Human)	IHC-P	Human
Park I, Larson PE, Zierhut ML et al. Hyperpolarized 13C magnetic resonance metabolic imaging: application to brain tumors. Neuro Oncol 2010-02-01 [PMID: 20150380] (IF/IHC)	IF/IHC
Arvidsson Y, Bergstrom A, Arvidsson L et al. Hypoxia stimulates CXCR4 signalling in ileal carcinoids. Endocr Relat Cancer 2010-06-01 [PMID: 20071457] (IF/IHC, Human)	IF/IHC	Human
Zhou GX, Ireland J, Rayman P et al. Quantification of carbonic anhydrase IX expression in serum and tissue of renal cell carcinoma patients using enzyme-linked immunosorbent assay: prognostic and diagnostic potentials. Urology 2010-02-01 [PMID: 19963243] (ELISA, Human)	ELISA	Human
Narita T, Yin S, Gelin CF et al. Identification of a Novel Small Molecule HIF-1{alpha} Translation Inhibitor. Clin Cancer Res 9;15(19):6128-6136. [PMID: 19789328] (WB, Human)	WB	Human
Lucio-Eterovic AK, Piao Y, de Groot JF. Mediators of Glioblastoma Resistance Invasion during Antivascular Endothelial Growth Factor Therapy. Clin Cancer Res:1078-0432.CCR-1009-0575. 2009-01-01 [PMID: 19567589] (IHC-P, Human)	IHC-P	Human
Loinard C, Ginouves A, Vilar J et al. Inhibition of prolyl hydroxylase domain proteins promotes therapeutic revascularization. Circulation 2009-07-01 [PMID: 19546390] (WB, Mouse, Human)	WB	Mouse, Human
Lee MH The immunological profile of renal cell carcinoma patients Thesis 2023-01-01 (IHC, Human)	IHC	Human
Hong YS, Cho HJ, Kim SY et al. Carbonic anhydrase 9 is a predictive marker of survival benefit from lower dose of bevacizumab in patients with previously treated metastatic colorectal cancer. BMC Cancer 2009-01-01 [PMID: 19619339] (IF/IHC, Human)	IF/IHC	Human
Li Y, Wang H, Oosterwijk E et al. Antibody-specific detection of CAIX in breast and prostate cancers. Biochem Biophys Res Commun 2009-08-28 [PMID: 19538935] (IHC-P, Human)	IHC-P	Human
Chen J, Kobayashi M, Darmanin S et al. Hypoxia-Mediated Up-Regulation of Pim-1 Contributes to Solid Tumor Formation. Am J Pathol;175(1):400-411. 2009-01-01 [PMID: 19528349] (IHC-P, Mouse)	IHC-P	Mouse
Di Napoli A, Signoretti S. Tissue biomarkers in renal cell carcinoma: issues and solutions. Cancer 2009-05-15 [PMID: 19402057] (Human)		Human
Banuelos CA, Banath JP, Kim J-Y et al. {gamma}H2AX Expression in Tumors Exposed to Cisplatin Fractionated Irradiation. Clin Cancer Res;15(10):3344-3353. 2009-01-01 [PMID: 19401347] (IHC-P, Human)	IHC-P	Human
Li Ying, Wang Hai, Oosterwijk Egbert et al. Expression and activity of carbonic anhydrase IX is associated with metabolic dysfunction in MDA-MB-231 breast cancer cells. Cancer Investigation 2009-01-01 [PMID: 19367501] (WB, Human)	WB	Human
Nakao M, Ishii G, Nagai K et al. Prognostic significance of carbonic anhydrase IX expression by cancer-associated fibroblasts in lung adenocarcinoma. Cancer 2009-06-15 [PMID: 19365853] (WB, Human)	WB	Human
Dumais J. Plant morphogenesis: a role for mechanical signals. Curr Biol 2009-03-10 [PMID: 19278636] (IF/IHC, Plant)	IF/IHC	Plant
Bachmann IM, Ladstein RG, Straume O et al. Tumor necrosis is associated with increased alphavbeta3 integrin expression and poor prognosis in nodular cutaneous melanomas. BMC Cancer 2008-01-01 [PMID: 19061491] (IF/IHC, Human)	IF/IHC	Human
Skapa P, Hyrsl L, Zavada J et al. Carbonic anhydrase IX expression in clear cell renal cell carcinomas negatively correlates with the proportion of the granular cell component. J Clin Oncol 2008-08-01 [PMID: 18669473] (Human)		Human
Sakata K, Someya M, Nagakura H et al. Brachytherapy for oral tongue cancer: an analysis of treatment results with various biological markers. Jpn J Clin Oncol;38(6):402-7. 2008-06-01 [PMID: 18573850] (IHC-P, Human)	IHC-P	Human
Al-Ahmadie HA, Alden D, Qin LX et al. Carbonic anhydrase IX expression in clear cell renal cell carcinoma: an immunohistochemical study comparing 2 antibodies. Am J Surg Pathol 2008-03-01 [PMID: 18300814] (IF/IHC, Human)	IF/IHC	Human
Baia GS, Dinca EB, Ozawa T et al. An orthotopic skull base model of malignant meningioma. Brain Pathol 2008-04-01 [PMID: 18093250] (IHC-P, Mouse)	IHC-P	Mouse
Gauter-Fleckenstein B, Fleckenstein K, Owzar K et al. Comparison of two Mn porphyrin-based mimics of superoxide dismutase in pulmonary radioprotection. Free Radic Biol Med 2008-03-15 [PMID: 18082148] (IHC-P, Rat)	IHC-P	Rat
Van den Eynden GG, Van der Auwera I, Van Laere SJ et al. Angiogenesis and hypoxia in lymph node metastases is predicted by the angiogenesis and hypoxia in the primary tumour in patients with breast cancer. Br J Cancer;93(10):1128-36. 2005-11-14 [PMID: 16251878] (IHC-P, Human)	IHC-P	Human
Chen L, Chen L, Huang J et al. Tyrosine kinase inhibitor sunitinib targets the vasculature of clear cell renal cell carcinoma: a morphometrical study of treatment effect. Int J Clin Exp Pathol. 2016-01-01 (IHC-P, Human)	IHC-P	Human
Kaidi A, Moorghen M, Williams AC et al. Is the downregulation of EphB2 receptor expression during colorectal tumorigenesis due to hypoxia?. Gut 56(11):1637-8. 2007-11-01 [PMID: 17938437] (IHC-P, Human)	IHC-P	Human
Westbury CB, Pearson A, Nerurkar A et al. Hypoxia can be detected in irradiated normal human tissue: a study using the hypoxic marker pimonidazole hydrochloride. Br J Radiol;80(959):934-8. 2007-11-01 [PMID: 17908818] (IHC-P, Human)	IHC-P	Human
Atkins M, Regan M, McDermott D et al. Carbonic anhydrase IX expression predicts outcome of interleukin 2 therapy for renal cancer. Clin Cancer Res;11(10):3714-21. 2005-05-15 [PMID: 15897568] (IF/IHC, Human)	IF/IHC	Human
Aubin RG Understanding the Cellular and Gene-Regulatory Mechanisms Underlying the Mesenchymal Transition of Ependymoma Tumor Cells Using Omics Approaches Thesis 2023-01-01
Yoo, H et al. Expression of the hypoxia marker carbonic anhydrase 9 is associated with anaplastic phenotypes in meningiomas. Clin Cancer Res;13 (1):68-75. 2007-01-01 [PMID: 17200340] (IHC-P, Human)	IHC-P	Human
Sathornsumetee S, Cao Y, Marcello JE et al. Tumor angiogenic and hypoxic profiles predict radiographic response and survival in malignant astrocytoma patients treated with bevacizumab and irinotecan. J Clin Oncol;26(2):271-8. 2008-01-10 [PMID: 1818266] (IHC-P, Human)	IHC-P	Human
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Reviews for Carbonic Anhydrase IX/CA9 Antibody (NB100-417) (7) 4.47

Average Rating: 4.4

(Based on 7 reviews)

We have 7 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-417:

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(3)

IHC-P

(2)

Immunofluorescence in frozen tissue/Immunofluorescence in vibratome cut tissue

(1)

ICC

(1)

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(5)

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reviewed by:
Daiana Drehmer

Human

05/31/2022

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Summary

Application	Western Blot
Sample Tested	breast cancer MCF7 cells,Cancer Cell lysate
Species	Human
Lot	AQ-2

Immunohistochemistry-Paraffin Carbonic Anhydrase IX/CA9 NB100-417

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reviewed by:
Lydia Taverne

IHC-P

Human

01/27/2020

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Summary

Application	Immunohistochemistry-Paraffin
Sample Tested	Stomach tissue
Species	Human
Lot	AK

Comments

Used citrate pH 6 antigen retrieval buffer to prepare the tissue. Applied the antibody at 1:250 concentration and detected with an AF546 labelled goat-anti-rabbit secondary antibody. Nuclei detected with DAPI.

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reviewed by:
Verified Customer

Immunofluorescence in frozen tissue/Immunofluorescence in vibratome cut tissue

Mouse

01/23/2019

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Summary

Application	Other
Sample Tested	P15 Mouse Brain
Species	Mouse
Lot	AB1

Comments

PFA fixed P15 mouse brains were cut 80µm using a vibratome and the sections were blocked in NGS (10%) in PBST (0.3% TritonX100) for 2 hrs at RT and incubated in primary antibody prepared in blocking buffer at 4 degrees overnight. after 3 washes in PBS (10 min each), the sections were stained in alexa-fluor conjugated secondary ab for 2 hrs at RT.

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reviewed by:
Verified Customer

IHC-P

Mouse

10/25/2017

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Summary

Application	Immunohistochemistry-Paraffin
Sample Tested	IHC on parrafin sections (mouse)
Species	Mouse
Lot	AD-1

reviewed by:
rajesha rupaimoole

Human

12/10/2014

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Summary

Application	Western Blot
Sample Tested	A2780 ovarian cancer cells
Species	Human

Western Blot Carbonic Anhydrase IX/CA9 NB100-417

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reviewed by:
Xiuquan Luo

Human

07/21/2014

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Summary

Application	Western Blot
Sample Tested	20 ug whole cell lysate
Species	Human

Immunocytochemistry Carbonic Anhydrase IX/CA9 NB100-417

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reviewed by:
Sonia Rocha

ICC

Human

12/21/2011

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Summary

Application	Immunocytochemistry
Sample Tested	Human cells
Species	Human

Product General Protocols

View specific protocols for Carbonic Anhydrase IX/CA9 Antibody (NB100-417):

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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FAQs for Carbonic Anhydrase IX/CA9 Antibody (NB100-417). (Showing 1 - 9 of 9 FAQs).

How many units do we need to purchase in order to stain 150 formalin fixed, paraffin embedded kidney biopsies? It depends on the amount used per slide, so could you please confirm how much antibody do you use per slide in IHC-P? Also, is the ab compatible with ENVISION FLEX+, HIGH pH, FOR AS?
- Unfortunately there is no number that I can provide to you as it is impossible to know for sure how many slides you will be able to stain with a vial. This will depend on your experimental conditions, your samples and how highly expressed the protein is, and what dilution you end up choosing. This antibody is recommended to be used at a 1:1000 dilution for IHC staining as this has worked well for our lab. You should order a single vial and test to determine what amount will be best for your assay rather than order too much or too little without first working with the antibody. As for your second question, that is not something that our lab has tested with so I am unsure if it is compatible or not. It has a fairly normal range pH in the buffer (7-8) so most likely the functions best around that range and not at a high pH.
Could you please let me know the immunogen peptide location for this Ab?
- The immunogen used to create this antibody was made to a cytoplasmic area of the protein.
I'm going to use your CA-9 Antibody for paraffin-Immunohistochemistry. Do I have to do any special pretreatments?
- We recommend antigen retrieval to ensure the epitope is not masked, and we also recommend that you add a permeablizing agent to access the epitope in the nucleus. A protocol specific for this product can be found under Product PDFs, labelled as "IHC Protocol".
We dilute our antibodies in 5% milk TBS-Tween and usually freeze-thaw them multiple times. Is this possible with this antibody when diluted?
- Freezing is not recommended for NB100-417 under any circumstances. Unfortunately, the antibody would no longer be covered by our guarantee if it was stored at a temperature that did not match our storage instructions. Of course, you are free to test your proposed conditions if you would like, but we would not be able to accept responsibility if the antibody was damaged by this.
Is this antibody is carrier-free? If not, do you have a carrier-free version?
- NB100-417 is carrier protein free, but does contain 0.1% sodium azide. The buffer formulation is: Tris-citrate/phosphate, pH 7-8 with 0.1% sodium azide. The following products are carrier protein and azide free (catalog numbers 23300002, H00000768-B01P and H00000768-D01P). If you would like to use NB100-417, we do offer antibody purification kits to remove unwanted buffer ingredients. See this link here for those products.
I have a couple of questions regarding the Carbonic Anhydrase IX Antibody (NB100-417) listed on your website. We are wondering if this antibody is carrier-free, and if not, whether you have a carrier-free version?
- NB100-417 is carrier protein free but does contain 0.025% sodium azide. The buffer formulation is: PBS with 0.025% sodium azide. The following products are carrier protein and azide free (catalog numbers NBP2-34411, NBP2-34408, and NBP2-33152). If you would like to use NB100-417, we do offer antibody purification kits to remove unwanted buffer ingredients.
I am interested in using the CA9 antibody (NB100-417) that you supply. We dilute our antibodies in 5% milk TBS-Tween and usually freeze-thaw them multiple times. Is this possible with this antibody when diluted?
- I have checked with the lab and they have informed me that freezing is not recommended for NB100-417 under any circumstances. Unfortunately, the antibody would no longer be covered by our guarantee if it was stored at a temperature that did not match our storage instructions. Of course, you are free to test your proposed conditions if you would like, but we would not be able to accept responsibility if the antibody was damaged by this.
Could this antibody (CA9) be safely stored at -80C?
- The recommended storage temperature for NB100-417 is -20C. It may be possible to store this antibody at -80C, but this has not been confirmed by our lab. It is most important to ensure that that antibody does not undergo too many freeze-thaw cycles.
What is the predicted theoretical molecular weight of Carbonic Anhydrase IX/CA9 antibodies?
- The TMW for Carbonic Anhydrase IX/CA9 antibodies is 50 kDa. Please note the observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Control Lysate(s)

HeLa Whole Cell Lysate
Carbonic Anhydrase IX/CA9 Overexpression Lysate

Secondary Antibodies

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Blogs on Carbonic Anhydrase IX/CA9.

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Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff... Read full blog post.

Carbonic anhydrase IX (CAIX) - a reliable histochemical marker of hypoxia
Carbonic anhydrase IX is a member of the carbonic anhydrase family. This family consists of catalytic enzymes capable of converting carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. This family of molecules is abundantly e... Read full blog post.

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Recent Reviews

4.4

5	4
4	2
3	1
2	0
1	0

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	Daiana Drehmer 05/31/2022
Application:	WB
Species:	Human

read this review

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	Lydia Taverne 01/27/2020
Application:	IHC-P
Species:	Human

read this review

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	Verified Customer 01/23/2019
Application:	Immunofluorescence in frozen tissue/Immunofluorescence in vibratome cut tissue
Species:	Mouse

read this review

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CA9

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

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Publications for Carbonic Anhydrase IX/CA9 Antibody (NB100-417)(271)

Reviews for Carbonic Anhydrase IX/CA9 Antibody (NB100-417) (7) 4.47

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Product General Protocols

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FAQs for Carbonic Anhydrase IX/CA9 Antibody (NB100-417). (Showing 1 - 9 of 9 FAQs).

Control Lysate(s)

Secondary Antibodies

Isotype Controls

Additional Carbonic Anhydrase IX/CA9 Products

Research Areas for Carbonic Anhydrase IX/CA9 Antibody (NB100-417)

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