EGLN1/PHD2 Antibody

PHD2 (Prolyl Hydroxylase Domain-containing protein 2) belongs to the Prolyl-4-hydroxylase domain (PHD) family of proteins and is encoded by the Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1) gene (1). Human EGLN1/PHD2 is a ubiquitously expressed enzyme that is 426 amino acids (aa) long with a theoretical molecular weight of ~46 kDa. Structurally PHD2 contains a nuclear export signal (NES, aa 6-20), an N-terminal MYND zinc finger domain (aa 21-58), and a C-terminal catalytic domain (aa 291-392) (2, 3). Functionally, PHD2 serves as an oxygen sensor and is responsible for post-translational modification of Hypoxia-inducible factor alpha (HIF-1alpha), a component of a transcriptional complex involved in oxygen homeostasis (1-3). During normoxia, PHD2 is responsible for oxygen-dependent hydroxylation of HIF-1alpha proline residue 402, 564, or both (3). The hydroxylation event promotes the binding of von Hippel-Lindau protein (VHL) and targets HIF1-alpha for ubiquitination and degradation (4, 5).

EGLN1/PHD2 has been implicated in several critical processes including erythropoiesis, angiogenesis, and metabolism as well as various pathologies such as cancer (2, 5, 6). Studies in mice have found that somatic deletion of PHD2 resulted in higher vascular endothelial growth factor A (VEGF-A) levels, increased blood vessel formation, and more erythropoietin (EPO), leading to severe polycythemia or erythrocytosis (high red blood cell (RBC) volume) (6). Another study revealed that specific point mutations in EGLN1/PHD2 led to elevated EPO and RBC mass associated with hemorrhages and strokes (6). Accordingly, given the known role of PHD2 in inhibition of EPO production, PHD2 inhibitors are being studied as a potential therapeutic for anemia (6). Additionally, dysregulation in EGLN1, and specifically the PHD2-VHL-HIF-1alpha pathway, has been associated with the development of pheochromocytomas (PCC) and sympathetic paragangliomas (PGL), which are rare neuroendocrine tumors (2). Besides pathological features, EGLN1/PHD2 may also be important for high altitude adaptation as two coding sequence variants in PHD2 are prevalent in the Tibetan population but is very rare in people at lower altitudes (2).

Alternate names for EGLN1/PHD2 include HIF Prolyl Hydroxylase 2, PH2, Prolyl hydroxylase domain containing protein 2, HIF2PH2, HIF-Prolyl hydroxylase 2, egl nine homolog 1, and C1orf12.

References

1. Amorim-Pires, D., Peixoto, J., & Lima, J. (2016). Hypoxia Pathway Mutations in Pheochromocytomas and Paragangliomas. Cytogenetic and genome research. https://doi.org/10.1159/000457479

2. Gardie, B., Percy, M. J., Hoogewijs, D., Chowdhury, R., Bento, C., Arsenault, P. R., Richard, S., Almeida, H., Ewing, J., Lambert, F., McMullin, M. F., Schofield, C. J., & Lee, F. S. (2014). The role of PHD2 mutations in the pathogenesis of erythrocytosis. Hypoxia (Auckland, N.Z.). https://doi.org/10.2147/HP.S54455

3. Minervini, G., Quaglia, F., & Tosatto, S. C. (2015). Insights into the proline hydroxylase (PHD) family, molecular evolution and its impact on human health. Biochimie. https://doi.org/10.1016/j.biochi.2015.07.009

4. Semenza G. L. (2007). Hypoxia-inducible factor 1 (HIF-1) pathway. Science's STKE : signal transduction knowledge environment. https://doi.org/10.1126/stke.4072007cm8

5. Chan, D. A., & Giaccia, A. J. (2010). PHD2 in tumour angiogenesis. British journal of cancer. https://doi.org/10.1038/sj.bjc.6605682

6. Meneses, A. M., & Wielockx, B. (2016). PHD2: from hypoxia regulation to disease progression. Hypoxia (Auckland, N.Z.). https://doi.org/10.2147/HP.S53576

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate.

We have publications tested in 7 applications: Block/Neutralize, ICC/IF, IF/IHC, IHC-P, IP, WB, Western Blot.

Showing Publications 1 - 10 of 92. Show All 92 Publications. Collapse Publications.

Publications using NB100-137	Applications	Species
Nicholas D Nolan, Xuan Cui, Brian M Robbings, Aykut Demirkol, Kriti Pandey, Wen-Hsuan Wu, Hannah F Hu, Laura A Jenny, Chyuan-Sheng Lin, Daniel T Hass, Jianhai Du, James B Hurley, Stephen H Tsang CRISPR editing of anti-anemia drug target rescues independent preclinical models of retinitis pigmentosa. Cell reports. Medicine 2024-04-19 [PMID: 38518771]
Lassi Luomala, Kalle Mattila, Paula Vainio, Harry Nisén, Teijo Pellinen, Jouni Lohi, Teemu D. Laajala, Petrus Järvinen, Anna‐Riina Koskenniemi, Panu Jaakkola, Tuomas Mirtti Low nuclear expression of HIF ‐hydroxylases PHD2 / EGLN1 and PHD3 / EGLN3 are associated with poor recurrence‐free survival in clear cell renal cell carcinoma Cancer Medicine 2024-02-24 [PMID: 38400673]
Sallais J, Park C, Alahari S et al. HIF1 inhibitor acriflavine rescues early-onset preeclampsia phenotype in mice lacking placental prolyl hydroxylase domain protein-2 JCI insight 2022-10-13 [PMID: 36227697] (WB, Mouse)	WB	Mouse
Schlegel C, Liu K, Spring B et al. Decreased expression of hypoxia-inducible factor 1 alpha (HIF-1 alpha) in cord blood monocytes under anoxia Pediatric research 2022-07-29 [PMID: 35906309] (WB, Human)	WB	Human
Bhute V, Harte J, Houghton J, Maxwell P Mannose binding lectin is hydroxylated by collagen prolyl-4-hydroxylase and inhibited by some PHD inhibitors Kidney360 2022-04-04 [PMID: 35368589]
Jian CB, Yu XE, Gao HD et al. Liposomal PHD2 Inhibitors and the Enhanced Efficacy in Stabilizing HIF-1alpha Nanomaterials (Basel, Switzerland) 2022-01-03 [PMID: 35010112] (WB, Human)	WB	Human
Li J, Lu X, Wei L Et al. PHD2 attenuates high-glucose-induced blood retinal barrier breakdown in human retinal microvascular endothelial cells by regulating the Hif-1 alpha/VEGF pathway Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2021-11-13 [PMID: 34773469] (WB, Human)	WB	Human
C Iacobini, M Vitale, G Pugliese, S Menini Normalizing HIF-1alpha Signaling Improves Cellular Glucose Metabolism and Blocks the Pathological Pathways of Hyperglycemic Damage Biomedicines, 2021-09-02;9(9):. 2021-09-02 [PMID: 34572324]
Kumar P, Verma V, Mohania D Et al. Leukemia associated RUNX1T1 gene reduced proliferation and invasiveness of glioblastoma cells Journal of cellular biochemistry 2021-08-09 [PMID: 34369622]
Marinaccio C, Suraneni P, Celik H et al. LKB1/STK11 Is a Tumor Suppressor in the Progression of Myeloproliferative Neoplasms Cancer Discovery 2021-06-01 [PMID: 33579786] (Western Blot, Block/Neutralize)	Western Blot, Block/Neutralize
Wang F, Yu H, Huang S et al. Jian-Pi-Yi-Shen Regulates EPO and Iron Recycling Protein Expressions in Anemic Rats with Chronic Kidney Disease: Accumulation of Hypoxia Inducible Factor-2 alpha via ERK Signaling Evid Based Complement Alternat Med 2020-11-12 [PMID: 33178327]
Shi M, Dai WQ, Jia RR et al. APCCDC20-mediated degradation of PHD3 stabilizes HIF-1a and promotes tumorigenesis in hepatocellular carcinoma Cancer Lett 2020-10-09 [PMID: 33039559] (IP, WB, Mouse)	IP, WB	Mouse
Mei H, Wu N, Huang X et al. Possible mechanisms by which silkworm faeces extract ameliorates adenine-induced renal anaemia in rats J Ethnopharmacol 2020-10-03 [PMID: 33022342] (WB, Rat)	WB	Rat
Sundaramurthi, H, Roche, S L Et al. Selective Histone Deacetylase 6 Inhibitors Restore Cone Photoreceptor Vision or Outer Segment Morphology in Zebrafish and Mouse Models of Retinal Blindness. Front Cell Dev Biol 2020-09-29 [PMID: 32984302] (IP, Mouse)	IP	Mouse
Dey A, Prabhudesai S, Zhang Y et al. Cystathione ?-synthase regulates HIF-1? stability through persulfidation of PHD2 Science Advances 2020-07-03 [PMID: 32937467] (Western Blot)	Western Blot
Settelmeier S, Schreiber T, MAki J et al. Prolyl hydroxylase domain 2 reduction enhances skeletal muscle tissue regeneration after soft tissue trauma in mice PLoS ONE 2020-05-15 [PMID: 32413092] (IF/IHC, Mouse)	IF/IHC	Mouse
Fang C, Manes TD, Liu L et al. A RASSF1A-HIF1 alpha loop drives Warburg effect in cancer and pulmonary hypertension Nat Commun 2019-05-13 [PMID: 31086178] (IP, Human)	IP	Human
Kurelac I, Iommarini L, Vatrinet R et al. Inducing cancer indolence by targeting mitochondrial Complex I is potentiated by blocking macrophage-mediated adaptive responses Nat Commun 2019-02-22 [PMID: 30796225] (WB, Human)	WB	Human
Miikkulainen P, Hogel H, Seyednasrollah F et al. Hypoxia-inducible factor (HIF)-prolyl hydroxylase 3 (PHD3) maintains high HIF2A mRNA levels in clear cell renal cell carcinoma. J. Biol. Chem. 2019-01-07 [PMID: 30617181]
Liu S, Zhang G, Guo J et al. Loss of Phd2 cooperates with BRAFV600E to drive melanomagenesis Nat Commun 2018-12-21 [PMID: 30575721] (IF/IHC, Human)	IF/IHC	Human
Deygas M, Gadet R, Gillet G et al. Redox regulation of EGFR steers migration of hypoxic mammary cells towards oxygen. Nat Commun. 2018-10-31 [PMID: 30382089] (WB, Human)	WB	Human
Kim G, Das R, Rao X et al. CITED2 restrains pro-inflammatory macrophage activation and response. Mol Cell Biol 2017-12-04 [PMID: 29203644] (WB, Mouse)	WB	Mouse
Hu J, Wang W, Zhang F et al. Hypoxia inducible factor-1 alpha mediates the profibrotic effect of albumin in renal tubular cells Sci Rep 2017-11-20 [PMID: 29158549] (WB, Rat)	WB	Rat
Miles AL, Burr SP, Grice GL, Nathan JA. The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1a prolyl hydroxylation by regulating cellular iron levels. Elife. 2017-03-15 [PMID: 28296633] (ICC/IF, Human)	ICC/IF	Human
Wottawa M, Naas S, Bottger J et al. Hypoxia-stimulated membrane trafficking requires T-plastin. Acta Physiol (Oxf) 2017-02-20 [PMID: 28218996]
Kozlova N, Wottawa M, Katschinski DM et al. Hypoxia-inducible factor prolyl hydroxylase 2 (PHD2) is a direct regulator of epidermal growth factor receptor (EGFR) signaling in breast cancer. Oncotarget 2017-02-07 [PMID: 28038470]
Burr SP, Costa AS, Grice GL et al. Mitochondrial Protein Lipoylation and the 2-Oxoglutarate Dehydrogenase Complex Controls HIF1a Stability in Aerobic Conditions. Cell Metab. 2016-11-08 [PMID: 27923773] (WB, Human)	WB	Human
Chen T, Zhou Q, et al. miR-17/20 Controls Prolyl Hydroxylase 2 (PHD2)/Hypoxia-Inducible Factor 1 (HIF1) to Regulate Pulmonary Artery Smooth Muscle Cell Proliferation. J Am Heart Assoc 2016-12-05 [PMID: 27919930] (WB, Mouse)	WB	Mouse
Lam YT, Lecce L, Tan JT et al. Androgen Receptor-Mediated Genomic Androgen Action Augments Ischemia-Induced Neovascularization. Endocrinology 2016-12-01 [PMID: 27754785]
Tao Y, Lin F, Li R et al. Prolyl hydroxylase-2 inhibits liver tumor cell proliferation and cyclin D1 expression in a hydroxylase-dependent manner. Int. J. Biochem. Cell Biol. 2016-06-13 [PMID: 27307407] (WB, Human)	WB	Human
Mysore VS, Szablowski J, Dervan PB, Frost PJ. A DNA-binding Molecule Targeting the Adaptive Hypoxic Response in Multiple Myeloma Has Potent Antitumor Activity. Mol Cancer Res. [PMID: 26801054] (WB, Human)	WB	Human
Sun W, Kosyna FK, Jelkmann W, Depping R. Prolyl-4-Hydroxylase 2 Potentially Contributes to Hepatocellular Carcinoma-Associated Erythrocytosis by Maintaining Hepatocyte Nuclear Factor-4alpha Expression. Cell. Physiol. Biochem. 2015-12-24 [PMID: 26624507] (WB, Human)	WB	Human
Vogler M, Zieseniss A, Hesse AR et al. Pre- and post-conditional inhibition of prolyl-4-hydroxylase domain enzymes protects the heart from an ischemic insult Pflugers Arch. 2015-01-13 [PMID: 25578858] (IHC-P, Mouse) Details: PHD3/HIF Prolyl Hydroxylase 3 antibody used at 1:10,000 dilution for IHC-P application on heart sections from C57BL/6 mice after 1, 6, and 24 h of treatment (40-fold magnification) with ICA, a specific PHD inhibitor 2-(1-chloro-4-hydroxyisoquinoline-3-carboxamido) acetate - two doses of 40 mg/kg BW ICA or vehicle i.p., either 6 and 1 h before myocardial infarction or 1 and 5 h after myocardial infarction (Fig 3).	IHC-P	Mouse
Bo H Role of oxygen on Nuclear factor erythroid-2-like 1 (NFE2L1 or Nrf1) function and stability Thesis (WB, Human)	WB	Human
Hogel H, Miikkulainen P, Bino L, Jaakkola PM. Hypoxia inducible prolyl hydroxylase PHD3 maintains carcinoma cell growth by decreasing the stability of p27. Mol. Cancer. 2015-07-30 [PMID: 26223520] (WB, Human) Details: PHD1 antibody used for WB on 786-O cell under hypoxia (1% O2) and normoxia (21 % O2) by siPHD1 (Figure 1C).	WB	Human
Ryou MG, Choudhury GR, Li W et al. Methylene blue-induced neuronal protective mechanism against hypoxia-reoxygenation stress Neuroscience. 2015-06-03 [PMID: 26047733] (WB, Human)	WB	Human
Pang X, Wang Z, Yin D, Zhang Z. Overexpression of hypoxia-inducible factor prolyl- hydoxylase attenuated by HCG-induced vascular endothelial growth factor expression in luteal cells. Mol Med Rep 2015-05-16 [PMID: 25975603]
Kumar P, Gullberg U, Olsson I, Ajore R. Myeloid translocation gene-16 co-repressor promotes degradation of hypoxia-inducible factor 1. PLoS ONE. 2015-05-15 [PMID: 25974097] (IP, WB, Primate, Human)	IP, WB	Primate, Human
Hsiao HW, Hsu TS, Liu WH et al. Deltex1 antagonizes HIF-1alpha and sustains the stability of regulatory T cells in vivo Nat Commun. 2015-02-20 [PMID: 25695215] (WB, Mouse, Human)	WB	Mouse, Human
Li J, Yuan W, Jiang S et al. Prolyl-4-hydroxylase Domain Protein 2 Controls NF-kB/p65 Transactivation and Enhances the Catabolic Effects of Inflammatory Cytokines on cells of the Nucleus Pulposus J. Biol. Chem. 2015-01-29 [PMID: 25635047] (ICC/IF, Rat)	ICC/IF	Rat
Henze At, Garvalov Bk, Seidel S et al. Loss of PHD3 allows tumours to overcome hypoxic growth inhibition and sustain proliferation through EGFR. Nat Commun. 2014-11-25 [PMID: 25420773]
Botting KJ, McMillen IC, Forbes H, Nyengaard, JR. Chronic hypoxemia in late gestation decreases cardiomyocyte number but does not change expression of hypoxia-responsive genes. J Am Heart Assoc. 2014-08-02 [PMID: 25085511] (WB)	WB
Sun W, Jelkmann W, Depping R. Prolyl-4-hydroxylase 2 enhances hypoxia-induced glioblastoma cell death by regulating the gene expression of hypoxia-inducible factor-a. Cell Death Dis 2014-07-11 [PMID: 25010988] (WB, Human) Details: PHD2/HIF Prolyl Hydroxylase 2 antibody used for WB at 1 : 500 dilution on 30-60ug lysates of U87MG, U138MG and U343MG cells (Figure 1 - wild type control and PHD2 knock-down cells), and in U87MG cells transfected with empty vector pcDNA or PHD2 plasmid (Figure 7 a- endogenous and over-expressed PHD2).	WB	Human
Lindholm ME, Fischer H, Poellinger L et al. Negative regulation of HIF in skeletal muscle of elite endurance athletes - a tentative mechanism promoting oxidative metabolism Am. J. Physiol. Regul. Integr. Comp. Physiol. 2014-06-04 [PMID: 24898836] (WB, Human) Details: PHD3/HIF Prolyl Hydroxylase 3 antibody used for WB on human biopsy samples (vastus lateralis muscle obtained via percutaneous needle technique) in experiments involving endurance-trained /elite cyclists-triathletes and moderately active men (Figures 2B, 4 )	WB	Human
Caprara V, Silvia S, Garrafa E et al. Endothelin-1 regulates hypoxia-inducible factor-1a and-2a stability through prolyl hydroxylase domain 2 inhibition in human lymphatic endothelial cells. Life sciences 2014-03-06 [PMID: 24607784] (WB, Human)	WB	Human
Place TL, Nauseef JT, Peterson MK et al. Prolyl-4-Hydroxylase 3 (PHD3) Expression Is Downregulated during Epithelial-to-Mesenchymal Transition. PLoS One 2013-12-18 [PMID: 24367580] (WB, Human)	WB	Human
Rawluszko AA, Bujnicka KE, Horbacka K et al. Expression and DNA methylation levels of prolyl hydroxylases PHD1, PHD2, PHD3 and asparaginyl hydroxylase FIH in colorectal cancer. BMC Cancer. 2013-11-06 [PMID: 24195777] (WB, Human)	WB	Human
Zhu Q, Hu J, Han WQ et al. Silencing of HIF Prolyl-Hydroxylase 2 Gene in the Renal Medulla Attenuates Salt-Sensitive Hypertension in Dahl S Rats. Am J Hypertens. 2013-11-04 [PMID: 24190904] (WB, Rat)	WB	Rat
Tan JT, Prosser HC, Vanags LZ et al. High-density lipoproteins augment hypoxia-induced angiogenesis via regulation of post-translational modulation of hypoxia-inducible factor 1alpha. FASEB J. 2013-09-10 [PMID: 24022405] (WB, Human)	WB	Human
Han WQ, Zhu Q, Hu J et al. Hypoxia-inducible Factor Prolyl-hydroxylase-2 Mediates Transforming Growth Factor Beta 1-induced Epithelial-mesenchymal Transition In Renal Tubular Cells. Biochim Biophys Acta 2013-03-01 [PMID: 23466866]
Kaufmann MR, Barth S, Konietzko U et al. Dysregulation of Hypoxia-Inducible Factor by Presenilin/gamma-Secretase Loss-of-Function Mutations. J Neurosci 2013-01-30 [PMID: 23365231] (WB, Human)	WB	Human
Wu S, Kasim V, Kano MR et al. Transcription factor YY1 contributes to tumor growth by stabilizing hypoxia factor HIF-1a in a p53-independent manner Cancer Res. 2013-03-15 [PMID: 23328582] (WB, Human)	WB	Human
Wottawa M, Leisering P, Ahlen Mv et al. Knockdown of prolyl-4-hydroxylase domain 2 inhibits tumor growth of human breast cancer MDA-MB-231 cells by affecting TGF-beta1 processing. Int J Cancer 2013-06-15 [PMID: 23225569] (WB, IF/IHC, Human)	WB, IF/IHC	Human
De Saedeleer CJ, Copetti T, Porporato PE et al. Lactate Activates HIF-1 in Oxidative but Not in Warburg-Phenotype Human Tumor Cells PLoS One 2012-01-01 [PMID: 23082126] (WB, Human)	WB	Human
Persano L, Pistollato F, Rampazzo E et al. BMP2 sensitizes glioblastoma stem-like cells to Temozolomide by affecting HIF-1 alpha stability and MGMT expression Cell Death Dis 2012-10-18 [PMID: 23076220] (WB, Human)	WB	Human
Verstraete M, Debucquoy A, Devos E et al. Investigation of possible endogenous hypoxia markers in colorectal cancer Int J Radiat Biol 2012-08-02 [PMID: 22852555] (IF/IHC, Human)	IF/IHC	Human
Fujita N, Markova D, Anderson DG, Chiba K, Toyama Y, Shapiro IM, Risbud MV. Expression of Prolyl Hydroxylases (PHDs) Is Selectively Controlled by HIF-1 and HIF-2 Proteins in Nucleus Pulposus Cells of the Intervertebral Disc: DISTINCT ROLES OF PHD2 AND PHD3 PROTEINS IN CONTROLLING HIF-1? ACTIVITY IN HYPOXIA. J Biol Chem;287(20):16975-86. 2012-05-11 [PMID: 22451659] (ICC/IF, WB, Rat, Human)	ICC/IF, WB	Rat, Human
Niecknig H, Tug S, Reyes BD, Kirsch M, Fandrey J. Berchner-Pfannschmidt U Role of reactive oxygen species in the regulation of HIF-1 by prolyl hydroxylase 2 under mild hypoxia. Free Radic Res. 2012-04-03 [PMID: 22360728] (WB, Human)	WB	Human
Kamphues C, Wittschieber D, Klauschen F et al. Prolyl hydroxylase domain 2 protein is a strong prognostic marker in human gastric cancer. Pathobiology 2012-01-01 [PMID: 22236543] (Human)		Human
Manalo DJ, Baek JH, Buehler PW et al. Inactivation of prolyl hydroxylase domain (PHD) protein by epigallocatechin (EGCG) stabilizes hypoxia-inducible factor (HIF-1[alpha]) and induces hepcidin (Hamp) in rat kidney. Biochemical and Biophysical Research Communications. 2011-11-25 [PMID: 22138393]
Hogel H, Rantanen K, Jokilehto T et al. Prolyl hydroxylase PHD3 enhances the hypoxic survival and G1 to S transition of carcinoma cells. PLoS One. 2011-01-01 [PMID: 22087251]
Wollenick K, Hu J, Kristiansen G et al. Synthetic transactivation screening reveals ETV4 as broad coactivator of hypoxia-inducible factor signaling. Nucleic Acids Res 2012-03-01 [PMID: 22075993]
Nandal A, Ruiz JC, Subramanian P, Ghimire-Rijal S, Sinnamon RA, Stemmler TL, Bruick RK, Philpott CC. Activation of the HIF prolyl hydroxylase by the iron chaperones PCBP1 and PCBP2. Cell Metab;14(5):647-57. 2011-11-02 [PMID: 22055506] (WB, Human)	WB	Human
Hemelrijk CK, Hildenbrandt H. Some causes of the variable shape of flocks of birds. PLoS One 2011-01-01 [PMID: 21829627]
Luo W, Hu H, Chang R et al. Pyruvate Kinase M2 is a PHD3-Stimulated Coactivator for Hypoxia-Inducible Factor 1. Cell;145(5):732-44. 2011-05-27 [PMID: 21620138] (WB, Human)	WB	Human
Brockmeier U, Platzek C, Schneider K et al. The function of hypoxia-inducible factor (HIF) is independent of the endoplasmic reticulum protein OS-9. PLoS One. 2011-04-29 [PMID: 21559462] (WB, Human)	WB	Human
Baek JH, Reiter CE, Manalo D et al. Induction of hypoxia inducible factor (HIF-1a) in rat kidneys by iron chelation with the hydroxypyridinone, CP94 Biochim Biophys Acta 2011-04-01 [PMID: 21558026] (WB, Human)	WB	Human
Fisher A, Hill C, Webber L et al. MVPA is associated with lower weight gain in 8-10 year old children: a prospective study with 1 year follow-up. PLoS One 2011-01-01 [PMID: 21552554] (Human)		Human
Wang Z, Tang L, Zhu Q et al. Hypoxia-inducible factor-1[alpha] contributes to the profibrotic action of angiotensin II in renal medullary interstitial cells. Kidney Int;79(3):300-310. 2011-02-01 [PMID: 20881940]
Bordoli MR, Stiehl DP, Borsig L et al. Prolyl-4-hydroxylase PHD2- and hypoxia-inducible factor 2-dependent regulation of amphiregulin contributes to breast tumorigenesis. Oncogene. 2011-02-03 [PMID: 20856199] (IHC-P, Human)	IHC-P	Human
Trisciuoglio Daniela, Gabellini Chiara, Desideri Marianna et al. Bcl-2 regulates HIF-1alpha protein stabilization in hypoxic melanoma cells via the molecular chaperone HSP90. PLoS One 2010-01-01 [PMID: 20668552] (WB, Human)	WB	Human
Jokilehto T. The Cellular Oxygen Sensor PHD2 in Cancer Growth 2011. Doctoral thesis, University of Turku, Finland.
Vogel S, Wottawa M, Farhat K et al. Prolyl hydroxylase domain (PHD) 2 affects cell migration and F-actin formation via RhoA/rho-associated kinase-dependent cofilin phosphorylation. J Biol Chem 2010-10-29 [PMID: 20801873] (WB, Human)	WB	Human
Jokilehto T, Hogel H, Heikkinen P et al. Retention of prolyl hydroxylase PHD2 in the cytoplasm prevents PHD2-induced anchorage-independent carcinoma cell growth Exp Cell Res 2010-04-01 [PMID: 20156434] (WB, IF/IHC, Human)	WB, IF/IHC	Human
Brokers N, Le-Huu S, Vogel S et al. Increased chemoresistance induced by inhibition of HIF-prolyl-hydroxylase domain enzymes. Cancer Sci 2010-01-01 [PMID: 19817749]
Luukkaa M, Jokilehto T, Kronqvist P et al. Expression of the cellular oxygen sensor PHD2 (EGLN-1) predicts radiation sensitivity in squamous cell cancer of the head and neck. Int J Radiat Biol. 2009-10-01 [PMID: 19639506] (IHC-P, Human)	IHC-P	Human
Chan DA, Kawahara TL, Sutphin PD et al. Tumor vasculature is regulated by PHD2-mediated angiogenesis and bone marrow-derived cell recruitment. Cancer Cell 2009-06-02 [PMID: 19477431]
Schultz K, Murthy V, Tatro JB et al. Prolyl hydroxylase 2 deficiency limits proliferation of vascular smooth muscle cells by hypoxia-inducible factor-1{alpha}-dependent mechanisms. Am J Physiol Lung Cell Mol Physiol;296(6):L921-927. 2009-01-01 [PMID: 19304911] (WB, Human)	WB	Human
Aprelikova O, Pandolfi S, Tackett S et al. Melanoma Antigen-11 Inhibits the Hypoxia-Inducible Factor Prolyl Hydroxylase 2 and Activates Hypoxic Response. Cancer Res 2009-01-15 [PMID: 19147576] (IP, WB, ICC/IF, Human)	IP, WB, ICC/IF	Human
Newpher TM, Ehlers MD. Glutamate receptor dynamics in dendritic microdomains. Neuron 2008-05-22 [PMID: 18498731] (Human)		Human
Rantanen K, Pursiheimo J, Hogel H, Himanen V, Metzen E, Jaakkola PM. Prolyl Hydroxylase PHD3 Activates Oxygen-dependent Protein Aggregation. Mol Biol Cell;19(5):2231-40. 2008-05-01 [PMID: 18337469] (ICC/IF, Human)	ICC/IF	Human
Barth, S, Nesper, J, Hasgall, P A, Wirthner, R, Nytko, K J, Edlich, F, Katschinski, D M, Stiehl, D P, Wenger, R H, Camenisch, G. The peptidyl-prolyl cis/trans isomerase FKBP38 determines HIF prolyl-4-hydroxylase PHD2 protein stability, MCB01324-01306. Mol Cell Biol27(10):3758-68. 2007-05-01 [PMID: 17353276] (WB, Human)	WB	Human
Yin, L, Kharba, S, Kufe, D. Mucin 1 Oncoprotein Blocks Hypoxia-inducible Factor 1{alpha} Activation in a Survival Response to Hypoxia, . J Biol Chem282(1):257-66. 2007-01-05 [PMID: 17102128] (WB, Human)	WB	Human
Li N et al. Expression actions of HIF prolyl-4-hydroxylase in the rat kidneys. Am J Physiol Renal Physiol 292: F207 - F216. 2007-01-01 [PMID: 16885149] (WB, IHC-P, Rat)	WB, IHC-P	Rat
McMahon, S et al. PROTEIN SYNTHESIS, POST-TRANSLATION MODIFICATION, DEGRADATION: TGF beta 1 induces HIF-1 stabilization through selective inhition of PHD2 expression. J Biol Chem, 10.1074/jbc.M604507200. 2006-01-01 [PMID: 16815840] (WB, Human)	WB	Human
Wu YL, Dudognon C, Nguyen E et al. Immunodetection of human telomerase reverse-transcriptase (hTERT) re-appraised: nucleolin and telomerase cross paths. J Cell Sci 2006-07-01 [PMID: 16772337]
Ietta, F et al. Dynamic HIF-1alpha regulation during human placental development. Biol Reprod. 2006-01-01 [PMID: 16611863] (WB, Human)	WB	Human
Elvidge, GP et al. Concordant regulation of gene expression by hypoxia 2-oxoglutarate depdendent dioxygenase inhibition; the role of HIF-1a, HIF-2a other pathways. J Biol Chem 281, 15215-15226. 2006-01-01 [PMID: 16565084] (WB, Human)	WB	Human
Jokilehto T, Rantanen K, Luukkaa M et al. Overexpression and nuclear translocation of hypoxia-inducible factor prolyl hydroxylase PHD2 in head and neck squamous cell carcinoma is associated with tumor aggressiveness. Clin Cancer Res 2006-02-01 [PMID: 16489060] (WB, IF/IHC, Human)	WB, IF/IHC	Human
Asikainen, TM et al. Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells explants of primate lung. PNAS 102(29): 10212-10217. 2005-01-01 [PMID: 16009933] (WB, Human)	WB	Human
Dalgard CL, Lu H, Mohyeldin A, Verma A. Endogenous 2-oxoacids differentially regulate expression of oxygen sensors. Biochem J 2004-06-01 [PMID: 14984367] (Human)		Human
Show All 92 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	Immunofluorescence and WB	Human and mouse liver cells	01/19/2020	View Summary Application Other Sample Tested Protein and paraffin sections Species Human and mouse liver cells Lot A1
Enlarge	5 reviewed by: John Collins	WB	Human	10/23/2017	View Summary Application Western Blot Sample Tested Human epithelial cell lines Species Human Lot A3
Enlarge	5 reviewed by: Verified Customer	WB	Human	10/08/2015	View Summary Application Western Blot Sample Tested OPM2 cells whole cell lysate Species Human Lot A3
Enlarge	5 reviewed by: Verified Customer	WB	Human	06/30/2015	View Summary Application Western Blot Sample Tested Cell lysates from prostate cancer cell lines Species Human Lot Lot A3
	4 reviewed by: Verified Customer	WB	Human	01/05/2015	View Summary Application Western Blot Sample Tested See PMID 23225569 Species Human
	4 reviewed by: Verified Customer	WB	Human	12/12/2014	View Summary Application Western Blot Sample Tested See PMID 23328582 Species Human
	5 reviewed by: Verified Customer	WB	Human	11/21/2014	View Summary Application Western Blot Species Human
	5 reviewed by: Verified Customer	WB	Other	09/22/2014	View Summary Application Western Blot Sample Tested Human SH-SY5Y or rat PC12 whole cell lysate. Species Other Lot A
	5 reviewed by: Verified Customer	WB	Other	08/24/2014	View Summary Application Western Blot Sample Tested SK-N-BE(2) whole cell lysate Species Other Lot A
Enlarge	5 reviewed by: Verified Customer	IF	Human	04/28/2014	View Summary Application Immunofluorescence Sample Tested U2OS (human osteosarcoma cell line) Species Human
Enlarge	5 reviewed by: Verified Customer	WB	Human	04/28/2014	View Summary Application Western Blot Sample Tested U87MG, U138MG, U343MG whole cell lysates Species Human
	5 reviewed by: Verified Customer	WB	Human	07/08/2010	View Summary Application Western Blot Sample Tested HeLa cell lysates, Sample Amount: 20 ug Species Human

Array NB100-137

• EGLN1/PHD2 Antibodies
• EGLN1/PHD2 Lysate
• EGLN1/PHD2 Proteins