HIF-1 alpha ELISA Kits: Novus Biologicals

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DYC1935-2

Human/Mouse Total HIF-1 alpha/HIF1A DuoSet IC ELISA, 2 Plate

(32 Publications)

1 Kit (for 2 Plates)

DYC1935-2

ELISA HIF-1 alpha [Biotin]

Lysates prepared from NIH-3T3 mouse embryonic fibroblast cells, untreated or treated with 150 µM of CoCl2, were incubated in wells coated with Human/Mouse Total HIF-1 alpha Capture Antibody. Unbound material was removed by washing, and bound material was solubilized in SDS gel sample buffer. Captured proteins were electrophoresed, transferred to PVDF membranes and immunoblotted with the Human/Mouse Total HIF-1 alpha Detection Antibody.

Lysates were prepared from MCF-7 human breast cancer cells, untreated or treated with 150 µM CoCl2. HIF-1 alpha was measured with the Human/Mouse Total HIF-1 alpha DuoSet IC ELISA and the same lysates were immunoblotted (inset) with Goat Anti-HIF-1 alpha Antibody (Catalog # AF1935). The DuoSet IC ELISA results correlate with the relative amounts of HIF-1 alpha detected by Western blot.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
Biotin	DYC1935E
Biotin	DYC1935-5

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