TOR/mTOR [p Ser2448] Antibody

Mammalian target of rapamycin (mTOR) is a serine and threonine protein kinase that regulates numerous cellular functions, in particular, the initiation of protein translation. Rapamycin is a natural product macrolide that induces G1 growth arrest in yeast, Drosophila, and mammalian cells. mTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, mTOR is a 245-kD protein referred to as RAFT1 with significant homology to the S. cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of mTOR to FKBP12-rapamycin correlates with the ability of these ligands to inhibit cell cycle progression.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: IF/IHC, IHC-P, WB.

Showing Publications 1 - 9 of 9.

Publications using NB600-607	Applications	Species
Serafin V, PorcU E, Cortese G et al. SYK Targeting Represents a Potential Therapeutic Option for Relapsed Resistant Pediatric ETV6-RUNX1 B-Acute Lymphoblastic Leukemia Patients Int J Mol Sci 2019-12-07 [PMID: 31817853] (WB, Human)	WB	Human
Cappoli N, Mezzogori D, Tabolacci E, Coletta I THE mTOR KINASE INHIBITOR RAPAMYCIN ENHANCES THE EXPRESSION AND RELEASE OF PRO-INFLAMMATORY CYTOKINE INTERLEUKIN 6 MODULATING THE ACTIVATION OF HUMAN MICROGLIAL CELLS EXCLI J 2019-10-25 [PMID: 31645839]
Lisi L, Ciotti GMP, Chiavari M et al. Phospho-mTOR expression in human glioblastoma microglia-macrophage cells Neurochem. Int. 2019-06-10 [PMID: 31195027] (WB, IF/IHC, Human)	WB, IF/IHC	Human
Rhee HJ, Shaib AH, Rehbach K et al. Pro-Inflammatory Activation of A New Immortalized Human Microglia Cell Line Brain Sci 2019-05-15 [PMID: 31096716] (WB, Human)	WB	Human
Zhou X, Yue GG, Chan AM et al. Eriocalyxin B, a novel autophagy inducer, exerts anti-tumor activity through the suppression of Akt/mTOR/p70S6K signaling pathway in breast cancer Biochem. Pharmacol. 2017-06-30 [PMID: 28669564] (IHC-P, Human)	IHC-P	Human
Lisi L, Laudati E, Navarra P, Dello Russo C. The mTOR kinase inhibitors polarize glioma-activated microglia to express a M1 phenotype. J Neuroinflammation 2014-07-23 [PMID: 25051975] (WB, Rat) Details: mTOR [p Ser2448] antibody used for WB on whole-cell lysates from rat's microglial cells activated by control-conditioned medium / C-CM or CM from C6 glioma cells activated with lipopolysaccharide/LPS or IFN-gamma (Figure 1).	WB	Rat
Lisi L, Stigliano E, Lauriola L et al. Proinflammatory-activated glioma cells induce a switch in microglial polarization and activation status, from a predominant M2b phenotype to a mixture of M1 and M2a/b polarized cells. ASN Neuro 2014-04-01 [PMID: 24689533] (WB, Rat)	WB	Rat
Lisi L, Navarra P, Feinstein DL, Dello Russo C. The mTOR kinase inhibitor rapamycin decreases iNOS mRNA stability in astrocytes. J Neuroinflammation 2011-01-05 [PMID: 21208419] (WB, Rat)	WB	Rat
Gupta M, Dillon SR, Ziesmer SC et al. A proliferation-inducing lig mediates follicular lymphoma B-cell proliferation cyclin D1 expression through phosphatidylinositol 3-kinase-regulated mammalian target of rapamycin activation. Blood;113(21):5206-5216. 2009-01-01 [PMID: 19321861]

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Lucia Lisi	WB	Human	01/17/2020	View Summary Application Western Blot Sample Tested Human microglia cell lysate Species Human Lot 29098
Enlarge	4 reviewed by: Lucia Lisi	IHC-P	Human	01/17/2020	View Summary Application Immunohistochemistry-Paraffin Sample Tested Human Brain Tissue Species Human Lot 29098

Array NB600-607

• TOR/mTOR Antibodies
• TOR/mTOR ELISA Kit
• TOR/mTOR Lysate
• TOR/mTOR Proteins