Glut1 Antibody - BSA Free

Glucose transporter 1 (GLUT1) or solute carrier family 2 (SLC2A1) is a member of the GLUT family of monosaccharides and polyols transporters. GLUT proteins transport glucose across cellular membranes through facilitative mechanisms and play a key role in glucose homeostasis (1). Fourteen GLUT proteins have been identified in the human, which are encoded by SLC2A genes 1-14 and are broadly expressed in many cell types and tissues. GLUT family members differ in sequence homology, substrate specificity and expression patterns. Based on sequence homology, GLUT family members are classified into Class I (GLUT1, 2, 3, 4, and GLUT14), Class II (GLUT5, 7, 9, and 11), and Class III (GLUT6, 8, 10, 12 and 13) (1). Structurally, GLUT transporters are integral membrane glycoproteins consisting of 12 membrane spanning helical domains, a single N-linked glycosylation site, and having cytoplasmic facing carboxy and amino terminal domains (2).

GLUT1 (Human glycosylated form theoretical molecular weight 55kDa) functions primarily as a glucose transporter but can transport other substrates including mannose, galactose and glucosamine across the membrane (3). Like other GLUT family members, GLUT1 is broadly expressed, nevertheless it is the predominant glucose transporter expressed in red blood cells and brain endothelial cells (1). SLC2A1 mutations underscore the autosomal dominant disorder GLUT1 deficiency syndrome (GLUTI-DS) which is characterized by low glucose levels in the brain or hypoglycorrhachia due to insufficient glucose transport across the blood brain barrier (2, 4, 5). Phenotypically, GLUT1-DS is characterized by early onset seizures, neurologic developmental delay, microcephaly, and ataxia (4). GLUT1 is highly expressed in the endothelium of cutaneous vascular lesions and serves as a marker for the diagnosis of juvenile or infantile hemangiomas (6).

References

1. Augustin, R. (2010). The protein family of glucose transport facilitators: It's not only about glucose after all. IUBMB Life. https://doi.org/10.1002/iub.315

2. Mueckler, M., & Thorens, B. (2013). The SLC2 (GLUT) family of membrane transporters. Molecular Aspects of Medicine. https://doi.org/10.1016/j.mam.2012.07.001

3. Stein, W. D., & Litman, T. (2015). Carrier-Mediated Transport. In Channels, Carriers, and Pumps. https://doi.org/10.1016/b978-0-12-416579-3.00004-6

4. Pearson, T. S., Akman, C., Hinton, V. J., Engelstad, K., & De Vivo, D. C. (2013). Phenotypic spectrum of glucose transporter type 1 deficiency syndrome (Glut1 DS). Current Neurology and Neuroscience Reports. https://doi.org/10.1007/s11910-013-0342-7

5. Messana, T., Russo, A., Vergaro, R., Boni, A., Santucci, M., & Pini, A. (2018). Glucose transporter type 1 deficiency syndrome: Developmental delay and early-onset ataxia in a novel mutation of the SLC2A1 gene. Journal of Pediatric Neurosciences. https://doi.org/10.4103/JPN.JPN_169_17

6. van Vugt, L. J., van der Vleuten, C. J. M., Flucke, U., & Blokx, W. A. M. (2017). The utility of GLUT1 as a diagnostic marker in cutaneous vascular anomalies: A review of literature and recommendations for daily practice. Pathology Research and Practice. https://doi.org/10.1016/j.prp.2017.04.023

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Rabbit.

We have publications tested in 12 applications: Block/Neutralize, Chemotaxis, FLOW, ICC/IF, IF/IHC, IHC-Fr, IHC-P, Immunocytochemistry/ Immunofluorescence, In vitro, PCR, WB, Western Blot.

Showing Publications 1 - 10 of 62. Show All 62 Publications. Collapse Publications.

Publications using NB110-39113	Applications	Species
Galiger C, Zohora FT, Dorneburg C et al. The survivin-ran inhibitor LLP-3 decreases oxidative phosphorylation, glycolysis and growth of neuroblastoma cells BMC cancer 2023-11-25 [PMID: 38007466]
Nishioku T, Anzai R, Hiramatsu S et al. Lactate dehydrogenase A inhibition prevents RANKL-induced osteoclastogenesis by reducing enhanced glycolysis Journal of Pharmacological Sciences 2023-12-01 [PMID: 37973217] (WB, Mouse)	WB	Mouse
Artico LL, Ruas JS, Teixeira Júnior JR et al. IGFBP7 Fuels the Glycolytic Metabolism in B-Cell Precursor Acute Lymphoblastic Leukemia by Sustaining Activation of the IGF1R-Akt-GLUT1 Axis International journal of molecular sciences 2023-06-02 [PMID: 37298628] (FLOW)	FLOW
Alexandria Béland-Millar, Alexia Kirby, Yen Truong, Julie Ouellette, Sozerko Yandiev, Khalil Bouyakdan, Chantal Pileggi, Shama Naz, Melissa Yin, Micaël Carrier, Pavel Kotchetkov, Marie-Kim St-Pierre, Marie-Ève Tremblay, Julien Courchet, Mary-Ellen Harper, Thierry Alquier, Claude Messier, Adam J Shuhendler, Baptiste Lacoste 16p11.2 haploinsufficiency reduces mitochondrial biogenesis in brain endothelial cells and alters brain metabolism in adult mice. Cell reports 2023-06-05 [PMID: 37149866]
Nsiah NY, Inman DM. Destabilizing COXIV in M�ller Glia Increases Retinal Glycolysis and Alters Scotopic Electroretinogram Cells 2022-11-24 [PMID: 36497016] (Immunocytochemistry/ Immunofluorescence)	Immunocytochemistry/ Immunofluorescence
Yeudall S, Upchurch CM, Seegren PV et al. Macrophage acetyl-CoA carboxylase regulates acute inflammation through control of glucose and lipid metabolism Science advances 2022-11-25 [PMID: 36417534] (WB, Mouse) Details: Dilution of 1:1000 was used	WB	Mouse
Bertelli PM, Pedrini E, Hughes D et al. Long term high glucose exposure induces premature senescence in retinal endothelial cells Frontiers in Physiology 2022-08-26 [PMID: 36091370]
Jassim AH, Nsiah NY, Inman DM. Ocular Hypertension Results in Hypoxia within Glia and Neurons throughout the Visual Projection Antioxidants (Basel) 2022-04-29 [PMID: 35624752]
He X, Zeng H, Cantrell AC et al. Knockout of TIGAR enhances myocardial phosphofructokinase activity and preserves diastolic function in heart failure Journal of Cellular Physiology 2022-08-01 [PMID: 35621078] (Western Blot, Block/Neutralize)	Western Blot, Block/Neutralize
Pham K, Hanaford AR, Poore BA et al. Comprehensive Metabolic Profiling of MYC-Amplified Medulloblastoma Tumors Reveals Key Dependencies on Amino Acid, Tricarboxylic Acid and Hexosamine Pathways Cancers 2022-03-03 [PMID: 35267619] (WB, Human)	WB	Human
García-Gómez P, Golán I, S Dadras M et al. NOX4 regulates TGF?-induced proliferation and self-renewal in glioblastoma stem cells Molecular oncology 2022-05-01 [PMID: 35203105] (WB, Human) Details: Multi-plex staining; Dilutions: 1:800	WB	Human
Wang X, Hu J, Fang Y et al. Multi-Omics Profiling to Assess Signaling Changes upon VHL Restoration and Identify Putative VHL Substrates in Clear Cell Renal Cell Carcinoma Cell Lines Cells 2022-01-29 [PMID: 35159281] (WB, Human)	WB	Human
Silva JVV, Ganesan S, Wickramasinghe HKJP Et al. Effects of branched-chain amino acids on glucose uptake and lactose synthesis rates in bovine mammary epithelial cells and lactating mammary tissue slices Journal of dairy science 2021-11-18 [PMID: 34802743]
Wessendarp M, Watanabe-Chailland M, Liu S Et al. Role of GM-CSF in regulating metabolism and mitochondrial functions critical to macrophage proliferation Mitochondrion 2021-11-01 [PMID: 34740864] (FLOW, Mouse)	FLOW	Mouse
Li Q Toll-like receptor 2/7 activation enhances the metabolism and functions of CD8+ T and B cells Thesis 2020-01-01 (FLOW, PCR, Mouse)	FLOW, PCR	Mouse
Jaiswal AK, Yadav J, Makhija S Et al. Short palate, lung, and nasal epithelial clone 1 (SPLUNC1) level determines steroid-resistant airway inflammation in aging Am J Physiol Lung Cell Mol Physiol 2021-12-01 [PMID: 34851736] (FLOW, Mouse) Details: Citation using the PE version of this antibody.	FLOW	Mouse
Pang L, Ng KT, Liu J et al. Plasmacytoid dendritic cells recruited by HIF-1 alpha /eADO/ADORA1 signaling induce immunosuppression in hepatocellular carcinoma Cancer letters 2021-09-16 [PMID: 34536555] (IF/IHC, Human)	IF/IHC	Human
Visavadiya NP, Rossiter HB, Khamoui AV. Distinct glycolytic pathway regulation in liver, tumour and skeletal muscle of mice with cancer cachexia Cell Biochemistry and Function 2021-08-01 [PMID: 34129243]
Shriwas P, Roberts D, Li Y et al. A small-molecule pan-class I glucose transporter inhibitor reduces cancer cell proliferation in vitro and tumor growth in vivo by targeting glucose-based metabolism Cancer & metabolism 2021-03-26 [PMID: 33771231] (IF/IHC)	IF/IHC
Abo El Gheit RE, Soliman NA, Badawi GA et al. Retinoprotective effect of agmatine in streptozotocin-induced diabetic rat model: avenues for vascular and neuronal protection : Agmatine in diabetic retinopathy Journal of physiology and biochemistry 2021-02-26 [PMID: 33635523] (IHC-P)	IHC-P
Johnson RM, Olatunde AC, Woodie LN et al. The Systemic and Cellular Metabolic Phenotype of Infection and Immune Response to Listeria monocytogenes Frontiers in Immunology 2021-02-08 [PMID: 33628207]
Balukoff NC, Ho JJD, Theodoridis PR et al. A translational program that suppresses metabolism to shield the genome Autophagy 2020-11-08 [PMID: 33188200]
Girotra M, Trachsel V, Roch A, Lutolf MP In Vivo Pre-Instructed HSCs Robustly Execute Asymmetric Cell Divisions In Vitro Int J Mol Sci 2020-11-06 [PMID: 33153113] (Mouse) Details: Citation using the PE version of this antibody.		Mouse
Okazaki Y, Murray J, Ehsani A et al. Increased glucose metabolism in Arid5b-/- skeletal muscle is associated with the down-regulation of TBC1 domain family member 1 (TBC1D1) Biol Res 2020-10-06 [PMID: 33023658]
Nesci V, Russo E, Arcidiacono B et al. Metabolic Alterations Predispose to Seizure Development in High-Fat Diet-Treated Mice: the Role of Metformin Mol. Neurobiol. 2020-08-12 [PMID: 32785826] (WB)	WB
Helou DG, Shafiei-Jahani P, Lo R et al. PD-1 pathway regulates ILC2 metabolism and PD-1 agonist treatment ameliorates airway hyperreactivity Nat Commun 2020-08-10 [PMID: 32778730] (Mouse) Details: Citation using the PE format of this antibody.		Mouse
Trachsel V In vitro fate mapping of stem cell development at the single cell level Thesis 2018-01-01 (FLOW, In vitro, Human) Details: The Phycoerythrin conjugated version of this antibody was used as part of this experiment, NB110-39113PE.	FLOW, In vitro	Human
Jaiswal AK, Makhija S, Stahr N Et al. Pyruvate kinase M2 in lung APCs regulates Alternaria-induced airway inflammation Immunobiology 2020-08-05 [PMID: 32747016] Details: Citation using the PE version of this antibody.
Cheung KCP, Fanti S, Mauro C et al. Preservation of microvascular barrier function requires CD31 receptor-induced metabolic reprogramming Nat Commun 2020-07-17 [PMID: 32681081] Details: Citation using the PE/Cy7 format of this antibody.
Zhang J, Zhang Y et al. LAIR-1 overexpression inhibits epithelial-mesenchymal transition in osteosarcoma via GLUT1-related energy metabolism. World J Surg Oncol 2020-06-20 [PMID: 32563267] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Ho JJD, Balukoff NC, Theodoridis PR et al. A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism Nat Commun 2020-05-29 [PMID: 32472050] (WB, Human) Details: Citation using the HRP format of this antibody.	WB	Human
Baumann T, Dunkel A, Schmid C et al. Regulatory myeloid cells paralyze T cells through cell-cell transfer of the metabolite methylglyoxal Nat. Immunol. 2020-05-01 [PMID: 32327756]
Staricha K, Meyers N, Garvin J et al. Effect of high glucose condition on glucose metabolism in primary astrocytes Brain Res. 2020-02-04 [PMID: 32032612] (WB, Rat)	WB	Rat
Galle-Treger L, Hurrell BP, Lewis G Autophagy is critical for group 2 innate lymphoid cell metabolic homeostasis and effector function J Allergy Clin Immunol. [PMID: 31738991] (FLOW, Mouse)	FLOW	Mouse
Bartel K, Muller R, von Schwarzenberg K Differential regulation of AMP-activated protein kinase in healthy and cancer cells explains why V-ATPase inhibition selectively kills cancer cells J Biol Chem. 2019-11-15 [PMID: 31604821] (WB, Human) Details: Citation used the HRP format of this antibody.	WB	Human
McGuire JL, Correll EA, Lowery AC et al. Pioglitazone improves working memory performance when administered in chronic TBI Neurobiol. Dis. 2019-09-09 [PMID: 31513844] (WB, Rat)	WB	Rat
Li X, Wen MZ, Su LX et al. Local suture ligation-assisted percutaneous sclerotherapy for Kasabach-Merritt phenomenon-associated kaposiform haemangioendothelioma. Oncol Lett 2019-01-01 [PMID: 30655857] (IHC-P, Human)	IHC-P	Human
Appel S, Grothe J, Storck S et al. A potential role for GSK3beta in glucose-driven intrauterine catch-up growth in maternal obesity Endocrinology 2018-12-10 [PMID: 30535296] (Mouse)		Mouse
Patel S, Fu S, Mastio J et al. Unique pattern of neutrophil migration and function during tumor progression. Nat. Immunol. 2018-11-01 [PMID: 30323345] (FLOW, Mouse)	FLOW	Mouse
Pacella I, Procaccini C, Focaccetti C et al. Fatty acid metabolism complements glycolysis in the selective regulatory T cell expansion during tumor growth Proc Proc Natl Acad Sci U S A. 2018-07-10 [PMID: 29941600] (FLOW, Mouse) Details: Citation using the PE form of this antibody.	FLOW	Mouse
Ashok A, Singh N Prion protein modulates glucose homeostasis by altering intracellular iron Scientific Reports 2018-04-26 [PMID: 29700330] (WB)	WB
Kishore M, Cheung KCP, Fu H et al. Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis Immunity. 2017-11-21 [PMID: 29669254] (FLOW, Mouse) Details: The Phycoerythrin conjugated version of this antibody was used as part of this experiment, NB110-39113PE.	FLOW	Mouse
Xu X, Cui N, Wang E. Application of an acellular dermal matrix to a rabbit model of oral mucosal defects Exp Ther Med 2018-01-05 [PMID: 29456650] (WB, Rabbit)	WB	Rabbit
Ho JJD, Balukoff NC, Cervantes G, Malcolm PD. Oxygen-Sensitive Remodeling of Central Carbon Metabolism by Archaic eIF5B. Cell Rep. 2018-01-02 [PMID: 29298419] (WB)	WB
Thwe P, Pelgrom L, Cooper R et al. Cell-Intrinsic Glycogen Metabolism Supports Early Glycolytic Reprogramming Required for Dendritic Cell Immune Responses. Cell Metab 2017 Sep 05 [PMID: 28877459] (FLOW, Mouse). 2017-09-05 [PMID: 28877459] (FLOW, Mouse) Details: This citation used the Alexa Fluor 488 form of this antbody	FLOW	Mouse
Cai TT, Ye SB, Liu YN et al. LMP1-mediated glycolysis induces myeloid-derived suppressor cell expansion in nasopharyngeal carcinoma PLoS Pathog. 2017-07-01 [PMID: 28732079] (ICC/IF, Human)	ICC/IF	Human
Kuhnel E, Kleff V, Stojanovska V et al. PlacentalSpecific Overexpression of sFlt1 Alters Trophoblast Differentiation and Nutrient Transporter Expression in an IUGR Mouse Model. Journal of Cellular Biochemistry. 2016-11-09 [PMID: 27859593] (WB, Mouse)	WB	Mouse
Nusken E, Gellhaus A, Kuhnel E et al. Increased Rat Placental Fatty Acid, But Decreased Amino Acid and Glucose Transporters Potentially Modify Intrauterine Programming. J. Cell. Biochem. 2015-11-21 [PMID: 26590355] (WB, Rat)	WB	Rat
Jacobs I, Hectors SJ, Schabel MC et al. Cluster analysis of DCE-MRI data identifies regional tracer-kinetic changes after tumor treatment with high intensity focused ultrasound. NMR Biomed. [PMID: 26390040] (IHC-Fr, Mouse) Details: Citation using the DyLight 650 form of this antibody.	IHC-Fr	Mouse
Wasser, Y, Y The Role of Dlk1 in the Gene Regulatory Network Underlying Motor Neuron Diversification Thesis 2022-01-01 (Western Blot, Mouse)	Western Blot	Mouse
Trompette A, Gollwitzer ES, Pattaroni C et al. Dietary Fiber Confers Protection against Flu by Shaping Ly6c- Patrolling Monocyte Hematopoiesis and CD8+ T Cell Metabolism Immunity 2018-05-15 [PMID: 29768180] (Mouse)		Mouse
De Riccardis L, Rizzello A, Ferramosca A et al. Bioenergetics profile of CD4(+) T cells in relapsing remitting multiple sclerosis subjects J. Biotechnol. 2015-02-19 [PMID: 25701681] (WB, Human) Details: Glut1 antibody used for WB analysis on CD4+ T cells from control and MS patients at a dilution of 1:1,000 with overnight incubation (Fig. 2D).	WB	Human
Tambe Y, Hasebe M, Kim CJ et al. The drs tumor suppressor regulates glucose metabolism via lactate dehydrogenase-B Mol. Carcinog. 2015-01-24 [PMID: 25620379] (WB, Mouse)	WB	Mouse
Yang F, Lu W, Lin W et al. Enhancement of Neurotrophic Factors in Astrocyte for Neuroprotective Effects in Brain Disorders Using Low-intensity Pulsed Ultrasound Stimulation. Brain Stimul. 2014-12-04 [PMID: 25558041] (WB, Human)	WB	Human
Rodriguez-Espinosa O, Rojas-Espinosa O, Moreno-Altamirano MM et al. Metabolic requirements for neutrophil extracellular traps nets) formation. Immunology 2014-12-27 [PMID: 25545227] (FLOW, Human) Details: Using the DyLight 650 conjugated version of NB110-39113, catalog number NB110-39113C.	FLOW	Human
Takeshita Y, Obermeier B, Cotleur A et al. An in vitro blood-brain barrier model combining shear stress and endothelial cell/astrocyte co-culture. J. Neurosci. Methods. 2014-05-22 [PMID: 24858797] (ICC/IF, Human)	ICC/IF	Human
Moreau JL, Artap ST, Shi H et al. Cited2 is required in trophoblasts for correct placental capillary patterning. Dev. Biol. 2014-05-05 [PMID: 24803182] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Luo W, Chang R, Zhong J, Pandey A, Semenza GL. Histone demethylase JMJD2C is a coactivator for hypoxia-inducible factor 1 that is required for breast cancer progression. Proc Natl Acad Sci U S A. 2012-12-04 [PMID: 23129632] (Chemotaxis)	Chemotaxis
Pena-Llopis S, Vega-Rubin-de-Celis S, Schwartz JC, et al. Regulation of TFEB and V-ATPases by mTORC1 EMBO J 2011-07-01 [PMID: 21804531] (WB, Mouse)	WB	Mouse
Luo W, Hu H, Chang R et al. Pyruvate Kinase M2 is a PHD3-Stimulated Coactivator for Hypoxia-Inducible Factor 1. Cell;145(5):732-44. 2011-05-27 [PMID: 21620138] (Chemotaxis, WB, Human)	Chemotaxis, WB	Human
Naga Prasad SV, Duan Z-H, Gupta MK et al. Unique MicroRNA Profile in End-stage Heart Failure Indicates Alterations in Specific Cardiovascular Signaling Networks. J Biol Chem;284(40):27487-27499. 2009-01-01 [PMID: 19641226] (WB, Human)	WB	Human
Hergovich A et al. Priming-dependent phosphorylation and regulation of the tumor suppressor pVHL by glycogen synthase kinase 3. Mol Cell Biol;26(15):5784-96. 2006-08-01 [PMID: 16847331] (WB, Human)	WB	Human
Show All 62 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Verified Customer	WB	Mouse	04/23/2019	View Summary Application Western Blot Sample Tested Mouse skeletal muscle Species Mouse Lot F-7
Enlarge	5 reviewed by: Verified Customer	ICC	Human	10/24/2017	View Summary Application Immunocytochemistry Sample Tested beta cells Species Human Lot F-2 Comments Comments use at 1:100
Enlarge	5 reviewed by: Ajay Ashok	WB	Human	06/12/2017	View Summary Application Western Blot Sample Tested Brain Species Human Comments Comments use at 1:500
Enlarge	5 reviewed by: Ajay Ashok	ICC	Mouse	06/12/2017	View Summary Application Immunocytochemistry Sample Tested mouse eye Species Mouse Comments Comments use at 1:100 concentration
Enlarge	5 reviewed by: Ajay Ashok	WB	Human and Mouse	06/12/2017	View Summary Application Western Blot Sample Tested Retina (inner nuclear layer) Species Human and Mouse Comments Comments Use at a conc of 1:500 for 20ug sample protein loaded
Enlarge	5 reviewed by: Marie-estelle LOSFELD	WB	Human	03/09/2014	View Summary Application Western Blot Sample Tested membrane fraction from lung cancer cells Species Human
Enlarge	5 reviewed by: Verified Customer	WB	Human	09/01/2010	View Summary Application Western Blot Sample Tested HeLa cell lysates, Sample Amount: 20 ug Species Human

Array NB110-39113

• Glut1 Antibodies
• Glut1 ELISA Kits
• Glut1 Lysate
• Glut1 Proteins