Imiquimod, TLR7 ligand

5 mg size will be provided as 5 x 1.0 mg vials.

Imiquimod, TLR7 ligand, an imidazoquinoline amine analog to guanosine, is an immune response modifier with potent indirect antiviral activity. The antiviral activity of imiquimod was first shown in guinea pigs infected with herpes simplex virus [1]. Imiquimod is now an approved treatment for external genital warts caused by human papillomavirus infection. This low molecular synthetic molecule induces the production of cytokines such as IFN-Alpha. Unlike R848, Imiquimod activates only TLR7 but not TLR8 [2]. This activation is MyD88-dependent and leads to the induction of the transcription factor NF-kB [3].

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 9 applications: Block/Neutralize, Flow Cytometry Control, Func, In Vivo, In vitro, In-vitro, Knockdown Validated, LA, Western Blot.

Showing Publications 1 - 10 of 13. Show All 13 Publications. Collapse Publications.

Publications using NBP2-26228	Applications	Species
Liu Y, Diamond SL. Activation of Most Toll-Like Receptors in Whole Human Blood Attenuates Platelet Deposition on Collagen under Flow Journal of Immunology Research 2023-01-17 [PMID: 36703865] (Block/Neutralize)	Block/Neutralize
Jalloh S, Olejnik J, Berrigan J et al. CD169-mediated restrictive SARS-CoV-2 infection of macrophages induces pro-inflammatory responses PLOS Pathogens 2022-10-24 [PMID: 36279285] (Western Blot, Knockdown Validated)	Western Blot, Knockdown Validated
Ye H, Pan J, Gong E et al. Inhibitory Effect of Immunologically Activated Mesenchymal Stem Cells on Lung Cancer Cell Growth and Metastasis Cancer biotherapy & radiopharmaceuticals 2021-03-26 [PMID: 33769841]
Effects of TLR Ligands on the Expression of Cytokines and Possible Role of NFkB in its Process in the Theca of Chicken Follicles Kang Y, Nii T, Isobe N J Poult Sci [PMID: 32055188] (Func, Chicken)	Func	Chicken
Hinks TSC, Marchi E, Jabeen M et al. Activation and In Vivo Evolution of the MAIT Cell Transcriptome in Mice and Humans Reveals Tissue Repair Functionality Cell Rep 2019-09-17 [PMID: 31533045] (In Vivo, Mouse, Human)	In Vivo	Mouse, Human
Parthasarathy G, Philipp MT. Intracellular TLR7 is activated in human oligodendrocytes in response to Borrelia burgdorferi exposure. Neurosci. Lett. 2018-02-03 [PMID: 29408631] (Human)		Human
Yabe-Wada T, Matsuba S, Takeda K et al. TLR signals posttranscriptionally regulate the cytokine trafficking mediator sortilin. Sci Rep. 2016-05-25 [PMID: 27220277] (Func, In vitro, Human, Mouse)	Func, In vitro	Human, Mouse
Li Q, Ma Y, Li L et al. Flagellin influences the expression of a variety of important cytokines and chemokines without affecting the immune status of umbilical cord mesenchymal stem cells. Mol Med Rep 2015-09-01 [PMID: 26330280] (In-vitro, Func)	In-vitro, Func
Nohmi K, Tokuhara D, Tachibana D et al. Zymosan Induces Immune Responses Comparable with Those of Adults in Monocytes, Dendritic Cells, and Monocyte-Derived Dendritic Cells from Cord Blood. J. Pediatr. 2015-05-06 [PMID: 25957979] (LA, In vitro, Func, Human) Details: Zymosan, TLR2 ligand (Imgenex IMG-2212) was used for in-vitro stimulation experiments involving human heparinized cord or adult blood Monocytes, peripheral blood dendritic cells (DCs) and monocyte-derived DCs (MoDCs). Zymosan was employed at 1 ug/mL concentration on Monocytes as well as on MoDCs and at 0.5 ug/mL on DCs. See full text for experimental details and results.	LA, In vitro, Func	Human
Zhang L, Liu D, Pu D et al. The TLR7 agonist Imiquimod promote the immunogenicity of msenchymal stem cells Biological Research. 2015-01-17 [PMID: 25654296] (Human) Details: Imiquimod, TLR7 ligand used for stimulating human umbilical cord mesenchymal stem cells (UCMSCs) in experiments involving study of the immunogenicity of MSCs- Imiquimod dissolved in DMSO at 2.5 mg/ml concentration to make a stock solution and then added the stock solution to 6 wells plates containing 1.5 x 10(5) cells in 2 ml medium at 10 ug/ml working concentration. Experiments also involved Imiquimod treatment to co-cultures of PBMC and UCMSC.		Human
Valencia Pacheco GJ, Pinzon Herrera F, Cruz Lopez JJ et al. Expression and activation of intracellular receptors TLR7, TLR8 and TLR9 in peripheral blood monocytes from HIV-infected patients. Colomb Med (Cali). 2013-06-30 [PMID: 24892454] (Flow Cytometry Control)	Flow Cytometry Control
Kusagaya H, Fujisawa T, Yamanaka K et al. Toll-like receptor-mediated airway IL-17C enhances epithelial host defense in an autocrine/paracrine manner. Am. J. Respir. Cell Mol. Biol. 2014-01-01 [PMID: 23944933] (In vitro, Human) Details: TLR ligand treatment (primary normal human bronchial epithelial cells), Figs 1, S1. CpG-ODN was used at 10 ug/ml.	In vitro	Human
Gillaux C, Mehats C, Vaiman D et al. Functional screening of TLRs in human amniotic epithelial cells. J Immunol. 2011-09-01 [PMID: 21775685] Details: TLR ligands: TLR1/2 (IMG-2201), TLR3 (IMG-2203), TLR4 (IMG-2204), TLR5 (IMG-2205), TLR6/2 (IMG-2206), TLR7 (IMG-2207), TLR9 (IMG-2209Hpt). The effects of ligand stimulation was measured by various readout assays, refer to the figures for details (Figs 2-8, S1).
Show All 13 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer			08/15/2017	View Summary Lot IMG-2207 Comments Comments Imiquimod was added into the medium at final concentration of 10 μg/ml. Imiquimod enhanced the osteo-differentiation ability of mesenchymal stem cells.

Array NBP2-26228

• Imiquimod, TLR7 ligand Support Product