CD8 Antibody (53-6.7) - BSA Free

CD8, also known as Leu-2 or T8 in human and Lyt2 or Lyt3 in mouse, is a cell surface glycoprotein belonging to the immunoglobulin supergene family (1, 2). CD8 is expressed on cytotoxic T-lymphocytes (T-cells), most thymocytes, between 35-45% of peripheral blood lymphocytes, and a population of natural killer (NK) cells (1, 2). The CD8 molecule consists of disulfide-linked alpha (alpha) and beta (beta) chains that present on T-cells as either CD8alphaalpha homodimers or CD8alphabeta heterodimers (1, 3). Both alpha and beta chains consist of a signaling sequence, an extracellular Ig-like domain, a membrane proximal stalk region, a transmembrane domain, and a cytoplasmic tail (3). Human CD8alpha is processed as 235 amino acids (aa) in length with a theoretical molecular weight of ~26 kDa, while mouse CD8alpha is 247 aa and has a theoretical molecular weight of 27.5 kDa (4, 5). Functionally, CD8 acts as an antigen coreceptor on cytotoxic T-cells and interacts with the major histocompatibility complex (MHC) class I molecules on antigen presenting cells (APCs), mediating cell-cell interactions within the immune system. Conversely, CD4 molecules interact with antigens presented on MHC class II molecules and are activated to become helper T-cells (TH) (1,2). Interestingly, thymocytes can transiently express both CD4 and CD8 during the maturation process (2). Furthermore, the cytoplasmic tail of CD8 has a Lck (lymphocyte-specific protein tyrosine kinase) binding domain where Lck interacts with CD8, initiating a phosphorylation cascade that activates transcription factors and promotes T-cell activation (6). More specifically, CD8alphabeta functions as a T-cell co-receptor, while CD8alphaalpha promotes T-cell survival and differentiation (7).

Given its role in the immune system, CD8-deficiency in T-cells is a hallmark of many diseases and pathologies (8-10). Specifically, CD8+ T-cell deficiency is prevalent in chronic autoimmune diseases including multiple sclerosis, rheumatoid arthritis, ulcerative colitis, Crohn's disease, type 1 diabetes mellitus, and Graves' disease (8). Furthermore, cancers or chronic infection can lead to CD8 T-cell exhaustion as the continual antigen presentation and inflammatory signals eventually cause the CD8+ T-cells to lose functionality (9, 10). However, animal models and clinical studies have suggested that T-cells are capable of being reinvigorated using inhibitory receptor blockade resulting in better disease outcomes and these exhausted T-cells may be a potential therapeutic target (9, 10).

Alternative names for CD8 includes CD antigen: CD8a, CD8 antigen, alpha polypeptide (p32), CD8a molecule, CD8A, Leu2 T-lymphocyte antigen, LEU2, MAL, OKT8 T-cell antigen, p32, T cell co-receptor, T8 T-cell antigen, T-cell antigen Leu2, T-cell surface glycoprotein CD8 alpha chain, and T-lymphocyte differentiation antigen T8/Leu-2.

References

1. Littman D. R. (1987). The structure of the CD4 and CD8 genes. Annual review of immunology. https://doi.org/10.1146/annurev.iy.05.040187.003021

2. Naeim F. (2008). Chapter 2- Principles of Immunophenotyping. Hematopathology. https://doi.org/10.1016/B978-0-12-370607-2.00002-8.

3. Gao, G. F., & Jakobsen, B. K. (2000). Molecular interactions of coreceptor CD8 and MHC class I: the molecular basis for functional coordination with the T-cell receptor. Immunology today. https://doi.org/10.1016/s0167-5699(00)01750-3

4. UniProt (P01732)

5. UniProt (P01731)

6. Kappes D. J. (2007). CD4 and CD8: hogging all the Lck. Immunity. https://doi.org/10.1016/j.immuni.2007.11.002

7. Gangadharan, D., & Cheroutre, H. (2004). The CD8 isoform CD8alphaalpha is not a functional homologue of the TCR co-receptor CD8alphabeta. Current opinion in immunology. https://doi.org/10.1016/j.coi.2004.03.015

8. Pender M. P. (2012). CD8+ T-Cell Deficiency, Epstein-Barr Virus Infection, Vitamin D Deficiency, and Steps to Autoimmunity: A Unifying Hypothesis. Autoimmune diseases. https://doi.org/10.1155/2012/189096

9. Kurachi M. (2019). CD8+ T cell exhaustion. Seminars in immunopathology. https://doi.org/10.1007/s00281-019-00744-5

10. Hashimoto, M., Kamphorst, A. O., Im, S. J., Kissick, H. T., Pillai, R. N., Ramalingam, S. S., Araki, K., & Ahmed, R. (2018). CD8 T Cell Exhaustion in Chronic Infection and Cancer: Opportunities for Interventions. Annual review of medicine. https://doi.org/10.1146/annurev-med-012017-043208

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, Immunocytochemistry/ Immunofluorescence.

Showing Publications 1 - 10 of 28. Show All 28 Publications. Collapse Publications.

Publications using NBP1-49045	Applications	Species
Zhou J, Wang H, Shu T et al. Myeloid-intrinsic cell cycle-related kinase drives immunosuppression to promote tumorigenesis iScience 2023-10-20 [PMID: 37731616]
Won S, Lee C, Bae S et al. Mass-produced gram-negative bacterial outer membrane vesicles activate cancer antigen-specific stem-like CD8(+) T cells which enables an effective combination immunotherapy with anti-PD-1 Journal of Extracellular Vesicles 2023-08-10 [PMID: 37563797] (Immunocytochemistry/ Immunofluorescence)	Immunocytochemistry/ Immunofluorescence
Son S, Nam J, Kim AS et al. Induction of T-helper-17-cell-mediated anti-tumour immunity by pathogen-mimicking polymer nanoparticles Nature biomedical engineering 2022-12-23 [PMID: 36564626] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Salman S, Meyers DJ, Wicks EE Et al. HIF inhibitor 32-134D eradicates murine hepatocellular carcinoma in combination with anti-PD1 therapy J Clin Invest 2022-05-02 [PMID: 35499076] (FLOW, Mouse) Details: Citation using the Alexa Fluor 488 version of this antibody.	FLOW	Mouse
Reyes AF, Goldusky J, Bhimalli P et al. Tracking fluorescently labeled IL-15 and anti-PD-1 in the tumor microenvironment and draining lymph nodes Journal of immunological methods 2022-03-28 [PMID: 35358495] (IF/IHC, Mouse)	IF/IHC	Mouse
Jie X, Chen Y, Zhao Y et al. Targeting KDM4C enhances CD8+ T cell mediated antitumor immunity by activating chemokine CXCL10 transcription in lung cancer Journal for immunotherapy of cancer 2022-02-01 [PMID: 35121645] (IF/IHC, Mouse)	IF/IHC	Mouse
Zhang Z, Na H, Gan Q Et al. Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease? Aging cell 2021-10-23 [PMID: 34687487] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Yang W, Feng Y, Zhou J et al. A selective HDAC8 inhibitor potentiates antitumor immunity and efficacy of immune checkpoint blockade in hepatocellular carcinoma Science translational medicine 2021-04-07 [PMID: 33827976]
Block MS, Dietz AB, Gustafson MP et al. Th17-inducing autologous dendritic cell vaccination promotes antigen-specific cellular and humoral immunity in ovarian cancer patients Nat Commun Oct 14 2020 [PMID: 33057068] (IHC, Human)	IHC	Human
Hoover AA, Hufnagel DH, Harris W et al. Lupeol Counteracts the Proinflammatory Signalling Triggered in Macrophages by 7-Keto-Cholesterol: New Perspectives in the Therapy of Atherosclerosis BMC Cancer 2020-09-27 [PMID: 33028251] (IHC-P, Mouse)	IHC-P	Mouse
Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130] (FLOW, Mouse) Details: Citation using the PE format of this antibody.	FLOW	Mouse
Cai EP, Ishikawa Y, Zhang W et al. Genome-scale in vivo CRISPR screen identifies RNLS as a target for beta cell protection in type 1 diabetes Nat Metab 2020-07-27 [PMID: 32719542]
Westrich JA, Vermeer DW, Silva A et al. CXCL14 suppresses human papillomavirus-associated head and neck cancer through antigen-specific CD8+ T-cell responses by upregulating MHC-I expression Oncogene 2019-08-15 [PMID: 31417179] (ICC/IF, Mouse)	ICC/IF	Mouse
Chakrabarti J, Holokai L, Syu L et al. Hedgehog signaling induces PD-L1 expression and tumor cell proliferation in gastric cancer. Oncotarget 2018-12-21 [PMID: 30647844]
Fazia T, Pastorino R, Notartomaso S et al. Acid Sensing Ion Channel 2: a new potential player in the pathophysiology of multiple sclerosis Eur. J. Neurosci. 2018-12-14 [PMID: 30549327] (IF/IHC, Mouse)	IF/IHC	Mouse
Samanta D, Park Y, Ni X et al. Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells Proc. Natl. Acad. Sci. U.S.A. 2018-01-24 [PMID: 29367423] (FLOW, Mouse) Details: This citation used the Alexa Fluor 488 form of this antbody	FLOW	Mouse
Sierra RA, Trillo-Tinoco J, Mohamed E et al. Anti-Jagged immunotherapy inhibits MDSCs and overcomes tumor-induced tolerance Cancer Res. 2017-09-13 [PMID: 28904063] (ICC/IF, Mouse)	ICC/IF	Mouse
Ma S, Deng W, Liu J et al. Blockade of adenosine A2A receptor enhances CD8+ T cells response and decreases regulatory T cells in head and neck squamous cell carcinoma. Mol Cancer 2017-06-07 [PMID: 28592285] (IF/IHC, Mouse)	IF/IHC	Mouse
Cohen N, Shani O, Raz Y et al. Fibroblasts drive an immunosuppressive and growth-promoting microenvironment in breast cancer via secretion of Chitinase 3-like 1. Oncogene. 2017-04-03 [PMID: 28368410] (ICC/IF, Mouse)	ICC/IF	Mouse
Fazio F, Zappulla C, Notartomaso S et al. Cinnabarinic acid, an endogenous agonist of type-4 metabotropic glutamate receptor, suppresses experimental autoimmune encephalomyelitis in mice. Neuropharmacology 2014-03-03 [PMID: 24565643] (IHC-P, Mouse)	IHC-P	Mouse
Mendes da Silva A Developing New Pharmacological Tools to Modulate Transposable Elements' Activity in Colorectal Cancer Thesis 2023-01-01 (ICC/IF)	ICC/IF
Li A, Wu Y, Tang F et al. In Vivo Magnetic Resonance Imaging of CD8+ T Lymphocytes Recruiting to Glioblastoma in Mice. Cancer Biother. Radiopharm. 2016-11-01 [PMID: 27831762] (Mouse)		Mouse
KheradmT, Wang S, Gibly R et al. Permanent protection of PLG scaffold transplanted allogeneic islet grafts in diabetic mice treated with ECDI-fixed donor splenocyte infusions Biomaterials 2011-07-01 [PMID: 21458857] (ICC/IF, IF/IHC, Mouse)	ICC/IF, IF/IHC	Mouse
Bankoti J, Burnett A, Navarro S et al. Effects of TCDD on the fate of naive dendritic cells. Toxicol Sci. 2010-06-01 [PMID: 20211938]
McLellan AD, Kapp M, Eggert A et al. Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression. Blood. 2002-03-15 [PMID: 11877283] (FLOW, Mouse) Details: This citation used the FITC version of this antibody.	FLOW	Mouse
Takahashi K, Nakata M, Tanaka T et al. CD4 and CD8 regulate interleukin 2 responses of T cells. Proc Natl Acad Sci U S A. 1992-06-01 [PMID: 1608966]
Ledbetter JA, Herzenberg LA. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979-01-01 [PMID: 398327]
Bhatia S Protease activated nanosensors for the noninvasive diagnosis of community-acquired pneumonia Thesis 2022-01-01 (IHC-Fr, Mouse)	IHC-Fr	Mouse
Show All 28 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	1 reviewed by: Marc Sherman	IHC-P	Mouse	03/02/2021	View Summary Application Immunohistochemistry-Paraffin Sample Tested FFPE mouse liver Species Mouse Lot FSGO62011A Comments Comments Attempted IF with this primary antibody against CD8 at concentrations 1:500, 1:200, 1:100, 1:50, 1:20, and 1:10. At concentrations >1:100 we were able to see dim positivity for IHC with HRP, but we were never able to visualize CD8 cells with immunofluorescence using either Alexa 488 and Alexa 468 secondaries (both secondaries confirmed working against other rat primaries). Unfortunately we needed to costain against two other targets so IHC alone didn't work for our purposes. ***Novus Response: Thank you for reviewing our product. We are sorry to that that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
Enlarge	5 reviewed by: Verified Customer	IHC-P	Mouse	10/23/2017	View Summary Application Immunohistochemistry-Paraffin Sample Tested mouse Head and neck squamous cell carcinoma (HNSCC) Species Mouse Lot NBP1-49045 Comments Comments sections were incubated with antibody for CD8 alpha (Novus, 1:200, for mouse HNSCC samples) at 4 °C for 12 h.
Enlarge	5 reviewed by: Naiche Adler	IHC-P	Mouse	12/15/2014	View Summary Application Immunohistochemistry-Paraffin Sample Tested Mouse Spleen Species Mouse

Array NBP1-49045

• CD8 Antibodies
• CD8 Antibody Pairs
• CD8 ELISA Kits
• CD8 Lysates
• CD8 Proteins
• CD8 Proteins and Enzymes
• CD8 ELISA