Western Blot: HSP70/HSPA1A Antibody [NBP1-77456] - Analysis of HSP70 in (1) human brain, (2) human liver, (3) human testes, (4) human skeletal muscle, and (5) human heart.
Immunohistochemistry: HSP70/HSPA1A Antibody [NBP1-77456] - Analysis of Hsp70 in human xenograft kidney cancer using DAB with hematoxylin counterstain.
Simple Western: HSP70/HSPA1A Antibody [NBP1-77456] - Lane view shows a specific band for Hsp70 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.
Hsp70 (Heat Shock 70kDa Protein 1A) belongs to a family of conserved ubiquitously expressed heat shock proteins with similar structure in virtually all living organisms. Hsp70 has plays an important role of the cell's machinery for protein folding. Hsp70 is also involved in stabilizing proteins against aggregation and allows them to refold by binding to hydrophobic residues exposed by stress. Additionally, Hsp70 directly inhibits heat-induced apoptosis.
Immunogen
A synthetic peptide made to a C-terminus portion of the human Hsp70 protein (between residues 600-641) [UniProt # P08107]
Localization
Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPA1A
Purity
Immunogen affinity purified
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Use in Human reported in scientific literature (PMID:33760187).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified
Alternate Names for HSP70/HSPA1A Antibody - BSA Free
dnaK-type molecular chaperone HSP70-1
FLJ54303
FLJ54370
FLJ54392
FLJ54408
FLJ75127
Heat shock 70 kDa protein 1/2
heat shock 70 kDa protein 1A/1B
heat shock 70kD protein 1A
heat shock 70kDa protein 1A
heat shock-induced protein
HSP70
HSP70.1/HSP70.2
HSP70-1
HSP70-1/HSP70-2
HSP70-1A
HSP70I
HSP72
HSPA1
HSPA1A
HSPA1B
Background
Hsp70 (Heat Shock 70kDa Protein 1A) belongs to a family of conserved ubiquitously expressed heat shock proteins with similar structure in virtually all living organisms. Hsp70 has plays an important role of the cell's machinery for protein folding. Hsp70 is also involved in stabilizing proteins against aggregation and allows them to refold by binding to hydrophobic residues exposed by stress. Additionally, Hsp70 directly inhibits heat-induced apoptosis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Aoki S, Harada K, Kawai S, et al. Expression of Heat Shock Proteins in Response to Mild Short-term Heat Shock in Human Deciduous Dental Pulp Fibroblast-like Cells Journal of Hard Tissue Biology 2021-01-26
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