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HSP70/HSPA1A Antibody - BSA Free

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Western Blot: HSP70/HSPA1A Antibody [NBP1-77456] - Analysis of HSP70 in (1) human brain, (2) human liver, (3) human testes, (4) human skeletal muscle, and (5) human heart.
Immunohistochemistry: HSP70/HSPA1A Antibody [NBP1-77456] - Analysis of Hsp70 in human xenograft kidney cancer using DAB with hematoxylin counterstain.
Simple Western: HSP70/HSPA1A Antibody [NBP1-77456] - Lane view shows a specific band for Hsp70 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Simple Western, Flow, IHC, MS, B/N
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

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HSP70/HSPA1A Antibody - BSA Free Summary

Background
Hsp70 (Heat Shock 70kDa Protein 1A) belongs to a family of conserved ubiquitously expressed heat shock proteins with similar structure in virtually all living organisms. Hsp70 has plays an important role of the cell's machinery for protein folding. Hsp70 is also involved in stabilizing proteins against aggregation and allows them to refold by binding to hydrophobic residues exposed by stress. Additionally, Hsp70 directly inhibits heat-induced apoptosis.
Immunogen
A synthetic peptide made to a C-terminus portion of the human Hsp70 protein (between residues 600-641) [UniProt # P08107]
Localization
Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPA1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Block/Neutralize reported in scientific literature (PMID 30417859)
  • Electron Microscopy reported in scientific literature (PMID 33335563)
  • Flow Cytometry reported in scientific literature (PMID 33760187)
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Simple Western 1:50
  • Western Blot 1:1000
Application Notes
This Hsp70 antibody is useful for Western blot and IHC-paraffin embedded sections.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
Human Liver Whole Tissue Lysate (Adult Whole Normal)
Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
Human Heart Whole Tissue Lysate (Adult Whole Normal)
Human Testis Whole Tissue Lysate (Adult Whole Normal)
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publications using
NBP1-77456 in the following applications:

Reactivity Notes

Use in Human reported in scientific literature (PMID:33760187).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HSP70/HSPA1A Antibody - BSA Free

  • dnaK-type molecular chaperone HSP70-1
  • FLJ54303
  • FLJ54370
  • FLJ54392
  • FLJ54408
  • FLJ75127
  • Heat shock 70 kDa protein 1/2
  • heat shock 70 kDa protein 1A/1B
  • heat shock 70kD protein 1A
  • heat shock 70kDa protein 1A
  • heat shock-induced protein
  • HSP70
  • HSP70.1/HSP70.2
  • HSP70-1
  • HSP70-1/HSP70-2
  • HSP70-1A
  • HSP70I
  • HSP72
  • HSPA1
  • HSPA1A
  • HSPA1B

Background

Hsp70 (Heat Shock 70kDa Protein 1A) belongs to a family of conserved ubiquitously expressed heat shock proteins with similar structure in virtually all living organisms. Hsp70 has plays an important role of the cell's machinery for protein folding. Hsp70 is also involved in stabilizing proteins against aggregation and allows them to refold by binding to hydrophobic residues exposed by stress. Additionally, Hsp70 directly inhibits heat-induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSP70/HSPA1A Antibody (NBP1-77456)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: B/N, FLOW, IHC-Fr, Immunohistochemistry, WB.


Filter By Application
B/N
(1)
FLOW
(1)
IHC-Fr
(1)
Immunohistochemistry
(1)
WB
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
Rat
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP1-77456 Applications Species
Chan L, Hsu W, Chen KY et al. Therapeutic Effect of Human Adipocyte-derived Stem Cell-derived Exosomes on a Transgenic Mouse Model of Parkinson's Disease In vivo (Athens, Greece) 2023-08-31 [PMID: 37652511] (WB, Human) WB Human
Ibrahim T, Wu P, Wang LJ et al. Sex-dependent differences in the genomic profile of lingual sensory neurons in na�ve and tongue-tumor bearing mice Scientific Reports 2023-08-12 [PMID: 37573456] (Immunohistochemistry) Immunohistochemistry
Ibrahim T, Wu P, Wang LJ et al. Sex-dependent Differences in the Genomic Profile of Lingual Sensory Neurons in Naïve and Tongue-Tumor Bearing Mice bioRxiv : the preprint server for biology 2023-05-14 [PMID: 36711730] (IHC-Fr, Mouse) IHC-Fr Mouse
Brewster LM, Coombs GB, Garcia VP et al. Effects of circulating extracellular microvesicles from spinal cord injured adults on endothelial cell function Clin. Sci. 2020-03-27 [PMID: 32219341] (Human) Human
Wu Hsing-Hsien, Huang Chien-Cheng, Chang Ching-Ping et al. Heat Shock Protein 70 (HSP70) Reduces Hepatic Inflammatory and Oxidative Damage in a Rat Model of Liver Ischemia/Reperfusion Injury with Hyperbaric Oxygen Preconditioning. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research 2018-11-12 [PMID: 30417859] (B/N, Rat) B/N Rat
Aoki S, Harada K, Kawai S, et al. Expression of Heat Shock Proteins in Response to Mild Short-term Heat Shock in Human Deciduous Dental Pulp Fibroblast-like Cells Journal of Hard Tissue Biology 2021-01-26
Chung CC, Chan L, Chen JH et al. Plasma extracellular vesicles tau and beta-amyloid as biomarkers of cognitive dysfunction of Parkinson's disease FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-10-01 [PMID: 34478572]
Show All 11 Publications.

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Bioinformatics

Gene Symbol HSPA1A
Uniprot