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HspA1L Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: HspA1L Antibody [NBP1-92012] - Analysis in human testis and endometrium tissues. Corresponding HspA1L RNA-seq data are presented for the same tissues.
Western Blot: HspA1L Antibody [NBP1-92012] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: HspA1L Antibody [NBP1-92012] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Western Blot: HspA1L Antibody [NBP1-92012] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). ...read more
Immunohistochemistry-Paraffin: HspA1L Antibody [NBP1-92012] - Staining of human endometrium shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: HspA1L Antibody [NBP1-92012] - Staining of human kidney shows no positivity in cells in tubules as expected.
Immunohistochemistry-Paraffin: HspA1L Antibody [NBP1-92012] - Staining of human prostate shows no positivity in glandular cells as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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HspA1L Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KLYQGGCTGPACGTGYVPGRPATGPTIEEVD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPA1L
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
HspA1L Protein (NBP1-92012PEP)
Publications
Read Publication using
NBP1-92012 in the following applications:

  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HspA1L Antibody

  • heat shock 10kDa protein 1-like
  • Heat shock 70 kDa protein 1-Hom
  • Heat shock 70 kDa protein 1L
  • heat shock 70 kDa protein 1-like
  • heat shock 70kD protein-like 1
  • heat shock 70kDa protein 1-like
  • HSP70-1L
  • HSP70-HOM
  • HSP70T
  • hum70t

Background

HSPA1L encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-92012
Species: Hu, Mu, Rt
Applications: WB, IHC

Publications for HspA1L Antibody (NBP1-92012)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IP, WB.


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(1)
WB
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for HspA1L Antibody (NBP1-92012). (Showing 1 - 1 of 1 FAQ).

  1. Does the protein array contain other HSP70 (HSP70/HSPA1A)?
    • The proteins used in their protein array analysis is proprietary; however based on a blast of the immunogen, the closest homology to any other HSP is 59% to HSPA2.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HSPA1L