HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
Concentration
5.0 mg/ml
Lysate Details for Array
Type
Tissue
Life Stage
Adult
Tissue
Heart
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole
Background
Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
Publications for Heart Lysate (NB820-59217)(7)
We have publications tested in 2 confirmed species: Human, Monkey.
We have publications tested in 3 applications: HPLC, MS, WB.
FAQs for Heart Lysate (NB820-59217). (Showing 1 - 4 of 4 FAQs).
Is this lysate a whole heart tissue or a particular heart myocard area - ventricle / atrium?
NB820-59217 is a lysate from the whole heart.
If I want to dilute the 20ug so it will give a volume larger than 4ul as supplied, what buffer do you recommend to use for that?
We recommend diluting in the buffer it comes supplied in, HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
How much protein do you recommend to use for each WB well for best results with a standard gel of 12 wells?
We recommend loading 10 to 20 ug per well.
I was looking at NB820-59217 and I would like to know if it is denatured at all?
They will not be native since they were lysed in this buffer:HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitorswith detergents
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