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HSP70/HSPA1A Antibodies

We offer HSP70/HSPA1A Antibodies for use in common research applications: Electron Microscopy, ELISA, Flow Cytometry, Gel Super Shift Assays, Immunoassay, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunomicroscopy, Immunoprecipitation, Simple Western, Western Blot. Each HSP70/HSPA1A Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our HSP70/HSPA1A Antibodies can be used in a variety of model species: Amphibian, Avian, Bacteria, Bovine, C. elegans, Canine, Chicken, Drosophila, E. coli, Fish, Guinea Pig, Hamster, Human, Insect, Monkey, Mouse, Plant, Porcine, Primate, Rabbit, Rat, Sheep, Xenopus, Yeast. Use the list below to choose the HSP70/HSPA1A Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our HSP70/HSPA1A polyclonal antibodies and browse our HSP70/HSPA1A monoclonal antibody catalog.

Alternate Names for HSP70/HSPA1A Antibodies

anti-HSP70/HSPA1A antibody, anti-HSPA1A antibody, anti-dnaK-type molecular chaperone HSP70-1 antibody, anti-FLJ54303 antibody, anti-FLJ54370 antibody, anti-FLJ54392 antibody, anti-FLJ54408 antibody, anti-FLJ75127 antibody, anti-Heat shock 70 kDa protein 1/2 antibody, anti-heat shock 70 kDa protein 1A/1B antibody, anti-heat shock 70kD protein 1A antibody, anti-heat shock 70kDa protein 1A antibody, anti-heat shock-induced protein antibody, anti-HSP70-1 antibody, anti-HSP70-1/HSP70-2 antibody, anti-HSP70-1A antibody, anti-HSP70.1/HSP70.2 antibody, anti-HSP70I antibody, anti-HSP72 antibody, anti-HSPA1 antibody, anti-HSPA1B antibody

25 Results for "hsp70-hspa1a" in Primary Antibodies

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MAB1663

HSP70/HSPA1A Antibody (242707) [Unconjugated]

(17 Publications)

100 μg

MAB1663

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with a 42 °C heat shock for 30 minutes with a 3 hour recovery. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB1663), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSP70/HSPA1A at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

HSP70/HSPA1A was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB1663) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) by heat shocked (HS), loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 67 kDa (as indicated) using 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB1663) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

KNK437 inhibition assay of cell cultures. (A) Viability test results of KNK437 (10, 50 and 100 μM) inhibition assay of mononuclear peripheral blood burst formation unit-erythroid (BFU-E) cultures, with and without EPO. (B) Viability test results of a KNK437 (50 and 100 μM) inhibition assay of CD34+ bone marrow BFU-E cultures, with and without EPO. (C) Viability test results of KNK437 treatment (50 μM) on HEL and Ba/F3 JAK2 V617F (BAF3MUT) cell lines. X axis: concentration (μM); Y axis: % of viability cells (viability test) or BFU-E (BFU-E cultures) (D) Molecular effects of KNK437 in HEL cell line Western blot of HSP90, HSP70, p-JAK2 (decreased with KNK437 treatment), p-ERK and p-P38. Actin was used as the housekeeping control. (E) Molecular effects of KNK437 in Ba/F3 cell line Western blot of JAK2, p-ERK, ERK, p-STAT5, STAT5 AND Actin, (F) Western blot of HSP90, HSP70 , JAK2, STAT5 and p-STAT5. Expression levels of HSP70, JAK2 and p-STAT5 decreased after HSP70 siRNA interference on HEL cell line. Actin was used as the housekeeping control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24252366), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1663-SP

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AF1663

HSP70/HSPA1A Antibody [Unconjugated]

(15 Publications)

50 μg

AF1663

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, L-929 mouse fibroblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat HSP70/HSPA1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1663) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HSP70/HSPA1A at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western shows lysates of Exosome Standards (HT‑29) (NBP3-11685), Exosome Standards (Human Urine) (NBP2-49840) and human liver tissue, loaded at 0.5 mg/ml. A specific band was detected for HSP70/HSPA1A at approximately 67 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human/Mouse/Rat HSP70/HSPA1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1663). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

<b>Biological Strategies Validation. </b>Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with heat shocked (HS), loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 66 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat HSP70/HSPA1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1663). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 66 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat HSP70/HSPA1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1663). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

<b>Biological Strategies Validation. </b>RFA increases HSP70 levels and activates MyD88. a C57BL/6 mice were exposed to RF and skin HSP70, HSc70, and HSP90 levels were then analyzed by western blotting at 6 and 24 h (hr) using GAPDH as internal control. b Representative IHC images of HSP70 expression in RF-treated and non-treated skin at 24 h. Scale: 250 µm. c WT and MyD88 KO mice were exposed to RF or sham treatment followed by ID injection of 10 µg OVA into RF or sham-treated skin. Serum anti-OVA antibody titer was measured 2 weeks later. n = 4–5. d WT mice were intradermally injected with 100 µg Pepinh-Control or Pepinh-MyD, or the same volume of PBS 3 and 1 h before RF treatment and ID OVA immunization at 10 µg dose. OVA immunization alone served as control (No adjuvant). Serum anti-OVA antibody titer was measured 2 weeks later. n = 4–5. One-way ANOVA with Tukey’s multiple comparison test was used to compare differences between groups in c and d. *p < 0.05; **p < 0.01. NS not significant. Representative of two independent experiments in c and d Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30209303), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1663-SP

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NB110-61582

HSP70/HSPA1A Antibody (C92F3A-5)

(1 Review)

(8 Publications)

0.2 mg

NB110-61582

Flow Cytometry: HSP70/HSPA1A Antibody (C92F3A-5) [NB110-61582] - Fluorescence Activated Cell Sorting analysis using Mouse Anti-Hsp70: FITC Monoclonal Antibody, Clone C92F3A-5 (NB110-61582). Tissue: Heat Shocked CD3+ CD8+ T cells . Species: Mouse. Primary Antibody: Mouse Anti-Hsp70: FITC Monoclonal Antibody (NB110-61582) at 1:1000. Courtesy of: Cheryl Cameron, Vaccine and Gene Therapy Instit. Florida.

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (C92F3A-5) [NB110-61582] - Tissue: Heat Shocked Melanoma cells. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 16 hours at RT. Secondary Antibody: Biotin Goat Anti-Mouse. Courtesy of: Dr. Ewa Malusecka, Maria Sklodowska-Curie Memorial Cancer Ceter and Inst. Of Oncology, Poland.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Ce, Ch, Dr, Fi, Gp, Ha, Pm, Rb, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ce, Ch, Dr, Fi, Gp, Ha, Pm, Rb, Sh

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP1-77456

HSP70/HSPA1A Antibody - BSA Free

(11 Publications)

0.1 ml

NBP1-77456

Simple Western: HSP70/HSPA1A Antibody [NBP1-77456] - Lane view shows a specific band for Hsp70 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77456AF350
Alexa Fluor 405	NBP1-77456AF405
Alexa Fluor 488	NBP1-77456AF488
Alexa Fluor 532	NBP1-77456AF532
Alexa Fluor 594	NBP1-77456AF594
Alexa Fluor 647	NBP1-77456AF647
Alexa Fluor 700	NBP1-77456AF700
Alexa Fluor 750	NBP1-77456AF750
Allophycocyanin	NBP1-77456APC
Biotin	NBP1-77456B
CoraFluor 1	NBP1-77456CL1
DyLight 350	NBP1-77456UV
DyLight 405	NBP1-77456V
DyLight 488	NBP1-77456G
DyLight 550	NBP1-77456R
DyLight 594	NBP1-77456DL594
DyLight 650	NBP1-77456C
DyLight 680	NBP1-77456FR
DyLight 755	NBP1-77456IR
FITC	NBP1-77456F
HRP	NBP1-77456H
Janelia Fluor 525	NBP1-77456JF525
Janelia Fluor 549	NBP1-77456JF549
Janelia Fluor 646	NBP1-77456JF646
Janelia Fluor 669	NBP1-77456JF669
PE	NBP1-77456PE
PerCP	NBP1-77456PCP
mFluor Violet 450 SE	NBP1-77456MFV450
mFluor Violet 500 SE	NBP1-77456MFV500
mFluor Violet 610 SE	NBP1-77456MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77456SS

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NBP1-77455

HSP70/HSPA1A Antibody - BSA Free

(1 Review)

(5 Publications)

0.1 ml

NBP1-77455

Flow Cytometry: HSP70/HSPA1A Antibody [NBP1-77455] - An intracellular stain was performed on Jurkat cells with HSP70/HSPA1A Antibody NBP1-77455AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: HSP70/HSPA1A Antibody [NBP1-77455] - Single cell suspension generated from B16-F10 tumor excised from C57BL/6 Mouse stained with Hsp70 & FITC secondary (blue) or unstained (red). [Image provided by a varified research professional; see Product Reviews for details on protocol employed].

Flow Cytometry: HSP70/HSPA1A Antibody [NBP1-77455] - An intracellular stain was performed on A549 cells with HSP70/HSPA1A Antibody NBP1-77455AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Simple Western: HSP70/HSPA1A Antibody [NBP1-77455] - Lane view shows a specific band for Hsp70 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Pm

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77455AF350
Alexa Fluor 405	NBP1-77455AF405
Alexa Fluor 488	NBP1-77455AF488
Alexa Fluor 532	NBP1-77455AF532
Alexa Fluor 594	NBP1-77455AF594
Alexa Fluor 647	NBP1-77455AF647
Alexa Fluor 700	NBP1-77455AF700
Alexa Fluor 750	NBP1-77455AF750
Allophycocyanin	NBP1-77455APC
Biotin	NBP1-77455B
CoraFluor 1	NBP1-77455CL1
DyLight 350	NBP1-77455UV
DyLight 405	NBP1-77455V
DyLight 488	NBP1-77455G
DyLight 550	NBP1-77455R
DyLight 594	NBP1-77455DL594
DyLight 650	NBP1-77455C
DyLight 680	NBP1-77455FR
DyLight 755	NBP1-77455IR
FITC	NBP1-77455F
HRP	NBP1-77455H
Janelia Fluor 525	NBP1-77455JF525
Janelia Fluor 549	NBP1-77455JF549
Janelia Fluor 646	NBP1-77455JF646
Janelia Fluor 669	NBP1-77455JF669
PE	NBP1-77455PE
PerCP	NBP1-77455PCP
mFluor Violet 450 SE	NBP1-77455MFV450
mFluor Violet 500 SE	NBP1-77455MFV500
mFluor Violet 610 SE	NBP1-77455MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77455SS

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NB110-96427

HSP70/HSPA1A Antibody (BB70)

(1 Review)

(5 Publications)

0.2 mg

NB110-96427

Western Blot: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - analysis of Bovine MDBK cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 . Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000.

Immunohistochemistry: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - Detection in paraffin embedded liver sections and nuclear sections of rat hepatocytes by staining with BB70. First pictures in series show two hours after exposure to stress, the second shows the control.

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Courtesy of: G. Matic, University of Belgrade, Serbia.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Fi, Gp, Ha, Rb, Sh, Xp, Ye

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Fi, Gp, Ha, Rb, Sh, Xp, Ye

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 405	NB110-96427AF405
Alexa Fluor 488	NB110-96427AF488
Alexa Fluor 647	NB110-96427AF647
Alexa Fluor 700	NB110-96427AF700
Biotin	NB110-96427B
DyLight 350	NB110-96427UV
DyLight 405	NB110-96427V
DyLight 488	NB110-96427G
DyLight 550	NB110-96427R
DyLight 650	NB110-96427C
DyLight 680	NB110-96427FR
DyLight 755	NB110-96427IR
FITC	NB110-96427F
HRP	NB110-96427H

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NBP1-21690

HSP70/HSPA1A Antibody (5A5)

(8 Publications)

0.1 mg

NBP1-21690

Reactivity:

Hu, Mu, Rt, Am, Ch, Dr, Fi, Ye

Species Glossary

Reactivity: Hu, Mu, Rt, Am, Ch, Dr, Fi, Ye

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 405	NBP1-21690AF405
Alexa Fluor 488	NBP1-21690AF488
Alexa Fluor 647	NBP1-21690AF647
Alexa Fluor 700	NBP1-21690AF700
Biotin	NBP1-21690B
CoraFluor 1	NBP1-21690CL1
DyLight 350	NBP1-21690UV
DyLight 405	NBP1-21690V
DyLight 488	NBP1-21690G
DyLight 550	NBP1-21690R
DyLight 650	NBP1-21690C
DyLight 680	NBP1-21690FR
DyLight 755	NBP1-21690IR
FITC	NBP1-21690F
HRP	NBP1-21690H
Janelia Fluor 525	NBP1-21690JF525
Janelia Fluor 549	NBP1-21690JF549
Janelia Fluor 646	NBP1-21690JF646
Janelia Fluor 669	NBP1-21690JF669
mFluor Violet 450 SE	NBP1-21690MFV450
mFluor Violet 500 SE	NBP1-21690MFV500
mFluor Violet 610 SE	NBP1-21690MFV610

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NBP2-02190

HSP70/HSPA1A Antibody (OTI3G9)

0.1 ml

NBP2-02190

Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded prostate tissue using anti-Hsp70 mouse monoclonal antibody.

Flow Cytometry: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190] - Analysis of permeabilized Jurkat cells, using anti-HSPA1A antibody( Red), compared to an isotype control (green), and a PBS control (blue).(1:100)

Western Blot: Hsp70 Antibody (3G9) [NBP2-02190] Analysis of extracts (35ug) from 9 different cell lines by using anti-Hsp70 monoclonal antibody.

Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Hsp70 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded endometrium tissue using anti-Hsp70 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded Kidney tissue using anti-Hsp70 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded liver tissue using anti-Hsp70 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded pancreas tissue using anti-Hsp70 mouse monoclonal antibody.

Reactivity:

Hu, Mu, Rt, Ca, Pm, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Pm, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-70954AF350
Alexa Fluor 405	NBP2-70954AF405
Alexa Fluor 488	NBP2-70954AF488
Alexa Fluor 532	NBP2-70954AF532
Alexa Fluor 594	NBP2-70954AF594
Alexa Fluor 647	NBP2-70954AF647
Alexa Fluor 700	NBP2-70954AF700
Alexa Fluor 750	NBP2-70954AF750
Allophycocyanin	NBP2-70954APC
Biotin	NBP2-70954B
CoraFluor 1	NBP2-70954CL1
DyLight 350	NBP2-70954UV
DyLight 405	NBP2-70954V
DyLight 488	NBP2-70954G
DyLight 550	NBP2-70954R
DyLight 594	NBP2-70954DL594
DyLight 650	NBP2-70954C
DyLight 680	NBP2-70954FR
DyLight 755	NBP2-70954IR
FITC	NBP2-70954F
HRP	NBP2-70954H
Janelia Fluor 525	NBP2-70954JF525
Janelia Fluor 549	NBP2-70954JF549
Janelia Fluor 646	NBP2-70954JF646
Janelia Fluor 669	NBP2-70954JF669
PE	NBP2-70954PE
PerCP	NBP2-70954PCP
mFluor Violet 450 SE	NBP2-70954MFV450
mFluor Violet 500 SE	NBP2-70954MFV500
mFluor Violet 610 SE	NBP2-70954MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-70954

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MAB16631

HSP70/HSPA1A Antibody (998953) [Unconjugated]

100 μg

MAB16631

Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 42° C heat shock for 30 minutes and allowed to recover for 3 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB16631) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HSP70/HSPA1A at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB16631) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HSP70/HSPA1A at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HSP70/HSPA1A was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB16631) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

HSP70/HSPA1A was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB16631) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 42oC heat shocked (HS) for 30 minutes and allowed to recover for 3 hours, loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 70 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB16631) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB16631-SP

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NB110-96425

HSP70/HSPA1A Antibody (N27F3-4)

(3 Publications)

0.2 mg

NB110-96425

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Ce, Ch, Dr, Fi, Gp, Ha, Pl, Pm, Rb, Sh, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ce, Ch, Dr, Fi, Gp, Ha, Pl, Pm, Rb, Sh, Xp

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 405	NB110-96425AF405
Alexa Fluor 488	NB110-96425AF488
Alexa Fluor 647	NB110-96425AF647
Alexa Fluor 700	NB110-96425AF700
Allophycocyanin	NB110-96425APC
Biotin	NB110-96425B
DyLight 350	NB110-96425UV
DyLight 405	NB110-96425V
DyLight 488	NB110-96425G
DyLight 550	NB110-96425R
DyLight 650	NB110-96425C
DyLight 680	NB110-96425FR
DyLight 755	NB110-96425IR
FITC	NB110-96425F
HRP	NB110-96425H
Janelia Fluor 525	NB110-96425JF525
Janelia Fluor 669	NB110-96425JF669
PE	NB110-96425PE
PerCP	NB110-96425PCP

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NBP1-97872

HSP70/HSPA1A Antibody

(1 Review)

(1 Publication)

0.05 mg

NBP1-97872

Reactivity:

Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, Dr, Fi, Gp, Ha, Pl, Pm, Sh, Ye

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, Dr, Fi, Gp, Ha, Pl, Pm, Sh, Ye

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-46806

HSP70/HSPA1A Antibody

(2 Publications)

0.1 ml

NBP2-46806

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody [NBP2-46806] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Western Blot: HSP70/HSPA1A Antibody [NBP2-46806] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10, Lane 2: Human cell line RT-4, Lane 3: Human cell line U-251 MG

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody [NBP2-46806] - Hsp70 / Hsc70 Antibody [NBP2-46806] - Staining of human testis shows strong cytoplasmic and nuclear positivity in subset of cells in seminiferus ducts.

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody [NBP2-46806] - Staining of human prostate shows moderate nuclear positivity in a subset of glandular cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

HSP70/HSPA1A Antibodies

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HSP70/HSPA1A Antibody - BSA Free

HSP70/HSPA1A Antibody - BSA Free

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HSP70/HSPA1A Antibody (5A5)

HSP70/HSPA1A Antibody (OTI3G9)

HSP70/HSPA1A Antibody (998953) [Unconjugated]

HSP70/HSPA1A Antibody (N27F3-4)

HSP70/HSPA1A Antibody

HSP70/HSPA1A Antibody