c-Fos Antibody (2H2)

c-Fos is an intermediate early gene that is one of four members of the FOS family of activator protein-1 (AP-1) transcription factors, which also includes Fra-1, Fra-2, and FosB (1-3). Under the FOS gene, human c-Fos is synthesized as a protein of 308 amino acids (aa) with a basic leucine zipper (bZip) domain important for dimerization, and a basic domain for interacting with DNA, with a theoretical molecular weight of ~40.6 kDa (4,5). c-Fos can heterodimerize with members of the JUN family (c-Jun, JunB, and JunD) to form the active transcription factor AP-1 complex (3,5). AP-1 related proteins bind TPA-related responsive elements (TRE), cAMP responsive elements (CRE), and related sequences, with c-Fos:c-Jun dimers partial to TRE sites (5).

In response to stimuli, c-Fos, which is encoded by protooncogenes, has a role in cell proliferation, differentiation, and transformation (3,6). A variety of stimuli can increase c-Fos expression such as growth factors, proinflammatory cytokines, UV radiation, neurotransmitters, hormones, injury, and stress (1,6). c-Fos has long been used as a marker for neuronal activity and is associated with neural and behavioral responses following stimuli (1-3, 6-7). Mouse studies have revealed that c-Fos is important for efficient neurogenesis and cortical development (3). Additionally, c-Fos signal can be used as a molecular marker for learning and memory, such as recognition and fear (2,7). Studies have found that repeated positive stimuli result in increased Fos expression while, conversely, repeated negative value stimuli are indicated by decreased signal (7). Intermediate early genes have also been implicated in neuropsychiatric disorders including showing altered c-Fos expression in a schizophrenia animal model (2). Furthermore, antipsychotics and antidepressants are both capable of impacting c-Fos expression (2).

References

1. Kovacs K. J. (1998). c-Fos as a transcription factor: a stressful (re)view from a functional map. Neurochemistry International. https://doi.org/10.1016/s0197-0186(98)00023-0

2. Gallo, F. T., Katche, C., Morici, J. F., Medina, J. H., & Weisstaub, N. V. (2018). Immediate Early Genes, Memory and Psychiatric Disorders: Focus on c-Fos, Egr1 and Arc. Frontiers in Behavioral Neuroscience. https://doi.org/10.3389/fnbeh.2018.00079

3. Velazquez, F. N., Caputto, B. L., & Boussin, F. D. (2015). c-Fos importance for brain development. Aging. https://doi.org/10.18632/aging.100862

4. Uniprot (P01100)

5. Wu, Z., Nicoll, M., & Ingham, R. J. (2021). AP-1 family transcription factors: a diverse family of proteins that regulate varied cellular activities in classical hodgkin lymphoma and ALK+ ALCL. Experimental Hematology & Oncology. https://doi.org/10.1186/s40164-020-00197-9

6. Shaulian, E., & Karin, M. (2001). AP-1 in cell proliferation and survival. Oncogene. https://doi.org/10.1038/sj.onc.1204383

7. Chung L. (2015). A Brief Introduction to the Transduction of Neural Activity into Fos Signal. Development & Reproduction. https://doi.org/10.12717/DR.2015.19.2.061

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC, IHC-Fr, WB.

Showing Publications 1 - 10 of 18. Show All 18 Publications. Collapse Publications.

Publications using NBP2-50037	Applications	Species
Zhai J, Li X, Hao H et al. Whisker Stimulation Alleviate ASD Behavior of BTBR Mice by Regulating Piezo2 Expression in DRG and Neuron Function in S1 and mPFC SSRN Electronic Journal 2022-12-20
Nagai Y, Kisaka Y, Nomura K et al. Dorsal raphe serotonergic neurons preferentially reactivate dorsal dentate gyrus cell ensembles associated with positive experience Cell reports 2023-02-22 [PMID: 36821440] (IHC, Mouse)	IHC	Mouse
Nagaoka K, Asaoka N, Nagayasu K et al. Enhancement of adenosine A2A signaling improves dopamine D2 receptor antagonist-induced dyskinesia via ?-arrestin signaling Frontiers in neuroscience 2023-01-24 [PMID: 36760795] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Bao C, Huang J, Wu H et al. Moxibustion alleviates depression-like behavior in rats with Crohn's disease by inhibiting the kynurenine pathway metabolism in the gut-brain axis Frontiers in neuroscience 2022-12-07 [PMID: 36570839] (WB, Rat)	WB	Rat
Kawai H, Bouchekioua Y, Nishitani N et al. Median raphe serotonergic neurons projecting to the interpeduncular nucleus control preference and aversion Nature communications 2022-12-22 [PMID: 36550097] (IHC-Fr, Mouse) Details: Dilution used in IHC-Fr 1:200	IHC-Fr	Mouse
Nagaoka K, Nagayasu K, Shirakawa H, Kaneko S Acetaminophen improves tardive akathisia induced by dopamine D2 receptor antagonists Journal of Pharmacological Sciences 2022-10-01 [PMID: 36522124] (IF/IHC, Rat)	IF/IHC	Rat
Takayama K, Tobori S, Andoh C et al. Autism Spectrum Disorder Model Mice Induced by Prenatal Exposure to Valproic Acid Exhibit Enhanced Empathy-Like Behavior via Oxytocinergic Signaling Biological & pharmaceutical bulletin 2022-07-31 [PMID: 35908894] (IHC-Fr, Mouse) Details: Dilution used for IHC 1:500	IHC-Fr	Mouse
Li Y, Zhu S, Xie K et al. TLR4 in Tph2 neurons modulates anxiety-related behaviors in a sex-dependent manner Neuropharmacology 2022-09-15 [PMID: 35787402] (ICC/IF) Details: Dilution: 1:1000	ICC/IF
Huang ST, Chen BB, Song ZJ et al. Unraveling the role of Epac1-SOCS3 signaling in the development of neonatal-CRD-induced visceral hypersensitivity in rats CNS neuroscience & therapeutics 2022-06-15 [PMID: 35702948] (IF/IHC, Rat)	IF/IHC	Rat
Figueroa C, Yang H, DiSpirito J Et al. Morphine exposure alters Fos expression in a sex-, age-, and brain region-specific manner during adolescence Developmental psychobiology 2021-09-01 [PMID: 34423851] (IF/IHC, Rat)	IF/IHC	Rat
Cao P, Chen C, Liu A Et Al. Early-life inflammation promotes depressive symptoms in adolescence via microglial engulfment of dendritic spines Neuron 2021-06-26 [PMID: 34233151]
Nagaoka K, Nagashima T, Asaoka N et al. Striatal TRPV1 activation by acetaminophen ameliorates dopamine D2 receptor antagonists-induced orofacial dyskinesia JCI insight 2021-04-15 [PMID: 33857021] (IF/IHC, Mouse)	IF/IHC	Mouse
Sun GL, Song ZJ, Peng XH et al. Projection-specific dopamine neurons in the ventral tegmental area participated in morphine-induced hyperalgesia and anti-nociceptive tolerance in male mice Journal of psychopharmacology (Oxford, England) 2021-03-21 [PMID: 33749357]
Hoshino N, Altarshan Y, Alzein A et al. Ephrin-A3 Is Required for Tonotopic Map Precision and Auditory Function in the Mouse Auditory Brainstem Research Square 2021-03-11 [PMID: 33731506]
Yoo H, Yang SH, Kim JY et al. Down-regulation of habenular calcium-dependent secretion activator 2 induces despair-like behavior Scientific reports 2021-02-12 [PMID: 33580180] (WB, Mouse)	WB	Mouse
Niu Y, Zeng X, Zhao L et al. Metabotropic glutamate receptor 5 regulates synaptic plasticity in a chronic migraine rat model through the PKC/NR2B signal The journal of headache and pain 2020-12-04 [PMID: 33276724] (WB, Rat)	WB	Rat
Bourgeois JR, Kalyanasundaram G, Figueroa C et al. A semi-automated brain atlas-based analysis pipeline for c-Fos immunohistochemical data J Neurosci Methods 2020-10-20 [PMID: 33091429] (IF/IHC, Rat)	IF/IHC	Rat
Beauchemin M, Geguchadze R, Guntur AR, et al. Exploring mechanisms of increased cardiovascular disease risk with antipsychotic medications: Risperidone alters the cardiac proteomic signature in mice Pharmacol. Res. 2019-12-23 [PMID: 31874253] (WB, Mouse)	WB	Mouse
Show All 18 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Peter Caradonna	ICC	Mouse	06/20/2017	View Summary Application Immunocytochemistry Sample Tested Adult spinal cord Species Mouse Comments Comments Immunofluorescence using NBP2-50037 at a dilution of 1:1000 on 4% PFA perfused 30 micron cryosections of mouse spinal cord. Tissue was blocked with 5% NGS/1% BSA/0.3% Triton X-100, then primary antibody was applied overnight at 4 C in the same diluent. Mouse on mouse avidin-biotin blocking kit with streptavidin-Alexa Fluor 488 conjugate was used for fluorescent detection.

Array NBP2-50037

• c-Fos Antibodies
• c-Fos Lysate
• c-Fos Proteins