Western Blot: HSP90 alpha Antibody [NB120-2928] - Analysis of 2-fold serial dilutions of HeLa cell lysate, starting at 10 ug, per well.
Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody [NB120-2928] - Analysis of Heat Shock Protein 86 (HSP86, green) in HeLa cells and NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton ...read more
Biological Strategies: Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Signaling consequences of STA-8666 treatment. HSP90 alpha. Magnification: 20x. Scale bars: 75um, except for vimentin, ...read more
Western Blot: HSP90 alpha Antibody [NB120-2928] - Figure 1 shows a Western blot of mouse HSP84 and HSP86 using NB120-2928 and NB120-2927 respectively.
Western Blot: HSP90 alpha Antibody [NB120-2928] - Analysis of epidermoid carcinoma (A431) cells using Heat Shock Protein 86 Polyclonal Antibody. Decreasing amounts of A431 whole cell lysates were probed with PA3-013 at ...read more
Western Blot: HSP90 alpha Antibody [NB120-2928] - Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP86 polyclonal antibody at a dilution of ...read more
Western Blot: HSP90 alpha Antibody [NB120-2928] - Analysis of 2-fold serial dilutions of HeLa cell lysate, starting at 10 ug, per well.
Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Both normal and cancer biopsies of deparaffinized Human kidney tissues.
Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Heat-induced epitope retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 20 mins at 95C. Following antigen retrieval, tissues blocked ...read more
Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.
Immunohistochemistry: HSP90 alpha Antibody [NB120-2928] - HSP90 alpha is upregulated in epithelial cells lining liver cysts.(A, B) Representative hematoxylin stained liver sections with immunohistochemical detection of ...read more
Immunohistochemistry: HSP90 alpha Antibody [NB120-2928] - HSP90 alpha is upregulated in epithelial cells lining liver cysts.(A, B) Representative hematoxylin stained liver sections with immunohistochemical detection of ...read more
Immunoprecipitation: HSP90 alpha Antibody [NB120-2928] - Analysis of Heat Shock Protein (HSP86) was performed on HeLa cells. Antigen-antibody complexes formed by incubating 500ug whole cell lysate with 2ug of HSP86 ...read more
Signaling consequences of STA-8666 treatment.A.-F. Left, Representative images and right, quantification of IHC or immunofluorescence for Ki-67 (A), cleaved caspase 3 (B), pS824-KAP1 (C), pT202/Y204-ERK1/2 (D), vimentin ...read more
IP: HSP 86 exists primarily as homodimers in HeLa cells. This antibody is capable of precipitating HSP 86 that is complexed with other proteins such as the aryl hydrocarbon (Ah) receptor.
Publications
Read Publications using NB120-2928 in the following applications:
Rabbit reactivity reported in scientific literature (PMID: 21129982).
Packaging, Storage & Formulations
Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified
Alternate Names for HSP90 alpha Antibody
FLJ31884
Heat shock 86 kDa
heat shock 90kD protein 1, alpha
heat shock 90kD protein 1, alpha-like 4
heat shock 90kD protein, alpha-like 4
heat shock 90kDa protein 1, alpha
heat shock protein 90kDa alpha (cytosolic), class A member 1
heat shock protein HSP 90-alpha
HSP86
Hsp89
HSP89A
HSP90 alpha
Hsp90
HSP90A
HSP90AA1
HSP90AHSP86
HSP90N
HSPC1
HSPC1HSP 86
HSPCA
HSPCAHSP89A
HSPCAL1
HSPCAL4
HSPN
LAP2
Renal carcinoma antigen NY-REN-38
Background
Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP 90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP 90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, Ah receptor, and some kinases. Studies have shown that murine HSP 90 exists as two forms, HSP 84 and HSP 86, coded by related but separate genes, with 86% homologous amino acid sequences. These forms are analogous to the two forms of human HSP 90, HSP 89 beta and HSP 89 alpha. In an unstressed mouse fibroblast, the basal level of HSP 84 is found to be double that of HSP 86. However, after heat shock, HSP 86 shows a greater increase. Studies also suggest that upon cellular differentiation, the level of HSP 86, but not HSP 84, decreases. HSP 84 and HSP 86, which may be subject to estrogenic regulation, have been found as components of the non-DNA binding form of mouse glucocorticoid receptor, but dissociated from the transformed DNA-binding form.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
=
÷
Review this Product
Be the first to review our HSP90 alpha Antibody and receive a gift card or discount.