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HSF1 Antibody

Images

 
Western Blot: HSF1 Antibody [NBP1-97475] - Analysis of HSF1: Lane 1: Molecular Weight Marker, Lane 2: HSF1 (human), (recombinant), Lane 3: HeLa Cell Lysate, Lane 4: HeLa Cell Lysate (Heat Shocked), Lane 5: 3T3 Cell ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ma, Pm, RbSpecies Glossary
Applications WB, ELISA, GS, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

HSF1 Antibody Summary

Immunogen
Recombinant human HSF1.
Specificity
Recognizes nonphosphorylated HSF1 at ~60kDa and phosphorylated HSF1 at ~80kDa.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSF1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Gel Super Shift Assays 1:1-1:100
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000 (colorimetric)
Application Notes
ELISA reactivity was reported in (PMID:12732732).
Theoretical MW
95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-97475 in the following applications:

  • 1 publication
  • GS
    2 publications
  • IP
    2 publications
  • WB
    5 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for HSF1 Antibody

  • heat shock factor protein 1
  • heat shock transcription factor 1HSTF1HSF 1
  • HSF1
  • HSTF 1
  • HSTF1

Background

HSFs (Heat Shock family of transcription factors), which consists of HSF 1-4, bind to highly conserved Heat shock elements (HSEs) in the promoter regions of heat shock genes, ultimately regulating the expression of Heat shock proteins (Hsps). On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleoplasmic distribution.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSF1 Antibody (NBP1-97475)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ELISA, GS, IP, WB.


Filter By Application
ELISA
(1)
GS
(2)
IP
(2)
WB
(5)
All Applications
Filter By Species
Human
(2)
All Species
Showing Publications 1 - 9 of 9.
Publications using NBP1-97475 Applications Species
Onay Ucar E, Sengelen A, Mertoglu Kamali E Hsp27, Hsp60, Hsp70, or Hsp90 depletion enhances the antitumor effects of resveratrol via oxidative and ER stress response in human glioblastoma cells Biochemical Pharmacology 2023-02-01 [PMID: 36603687] (WB, Human)

Details:
1:1000 WB dilution
WB Human
Kiyga E, Adıguzel Z, Onay Ucar E Temozolomide increases heat shock proteins in extracellular vesicles released from glioblastoma cells Molecular biology reports 2022-06-25 [PMID: 35752701] (WB, Human) WB Human
TW Mu et al. Chemical and biological approaches synergize to ameliorate protein-folding diseases. Cell 134, 769 . 2008-01-01 [PMID: 18775310] (WB) WB
G Steiner et al. TNFalpha mediates susceptibility to heat-induced apoptosis by protein phosphatase-mediated inhibition of the HSF1/hsp70 stress response:; . Cell Death Differ 10, 1126. 2003-01-01 [PMID: 14502236] (GS) GS
DM Chuang et al. Postinsult treatment with lithium reduces brain damage and facilitates neurological recovery in a rat ischemia/reperfusion model:; . PNAS 100, 6210. 2003-01-01 [PMID: 12732732] (ELISA) ELISA
FR Sharp et al. 17-beta-estradiol induces heat shock proteins in brain arteries and potentiates ischemic heat shock protein induction in glia and neurons. J. Cereb. Blood Flow Metab. 22, 183. 2002-01-01 [PMID: 11823716] (WB) WB
M Di Rosa et al. Role of cyclopentenone prostaglandins in rat carrageenin pleurisy:;. FEBS Lett 508, 61. 2001-01-01 [PMID: 11707269] (GS, WB) GS, WB
R Voellmy et al. Evidence for a mechanism of repression of heat shock factor 1 transcriptional activity by a multichaperone complex:; J. Biol Chem 276, 45791. 2001-01-01 [PMID: 11583998] (IP) IP
DS Latchman et al. Signal transducer and activator of transcription-1 and heat shock factor-1 interact and activate the transcription of the Hsp-70 and Hsp-90 beta gene promoters; J. Biol Chem 274, 1723. 1999-01-01 [PMID: 9880553] (IP) IP

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HSF1
Entrez
Uniprot