HO-1/HMOX1/HSP32 Antibody (HO-1-1)

Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ICC/IF, IF/IHC, IHC, IHC-P, Simple Western, WB.

Showing Publications 1 - 10 of 20. Show All 20 Publications. Collapse Publications.

Publications using NBP1-97507	Applications	Species
Peng R, Liu X, Wang C et al. Iron overload enhances TBI-induced cardiac dysfunction by promoting ferroptosis and cardiac inflammation Biochemical and biophysical research communications 2023-11-19 [PMID: 37801989] (IHC, Mouse)	IHC	Mouse
Hung YW, Ouyang C, Ping X et al. Extracellular arginine availability modulates eIF2? O-GlcNAcylation and heme oxygenase 1 translation for cellular homeostasis Journal of biomedical science 2023-05-22 [PMID: 37217939] (IHC-P, WB, ICC/IF, Human)	IHC-P, WB, ICC/IF	Human
Yoo MH, Lee SJ, Kim W et al. Bisphenol A impairs renal function by reducing Na+/K+-ATPase and F-actin expression, kidney tubule formation in vitro and in vivo Ecotoxicology and environmental safety 2022-10-04 [PMID: 36206637] (IF/IHC, ICC/IF, Rat, Human)	IF/IHC, ICC/IF	Rat, Human
Rai D, Tripathi AK, Sardar A et al. A novel BMP2 secretagogue ameliorates glucocorticoid induced oxidative stress in osteoblasts by activating NRF2 dependent survival while promoting Wnt/ beta-catenin mediated osteogenesis Free radical biology & medicine 2022-08-10 [PMID: 35963563] (WB, Mouse)	WB	Mouse
Arigela CS, Nelli G, Gan SH Et al. Bitter Gourd Honey Ameliorates Hepatic and Renal Diabetic Complications on Type 2 Diabetes Rat Models by Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Mechanisms Foods (Basel, Switzerland) 2021-11-20 [PMID: 34829154] (ICC/IF, Rat)	ICC/IF	Rat
Vendidandala NR, Yin TP, Nelli G Et al. Gallocatechin silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-kappa B pathways Life sciences 2021-10-06 [PMID: 34624322] (IHC-P, Rat)	IHC-P	Rat
Nesbitt NM, Malone LE, Liu Z et al. Divergent erythroid megakaryocyte fates in Blvrb-deficient mice establish non-overlapping cytoprotective functions during stress hematopoiesis Free radical biology & medicine 2020-12-24 [PMID: 33359909] (WB, Mouse)	WB	Mouse
Seimetz M, Sommer N, Bednorz M et al. NADPH oxidase subunit NOXO1 is a target for emphysema treatment in COPD Nat Metab 2020-06-01 [PMID: 32694733]
Seok J, Park H, Choi JH et al. Placenta-Derived Mesenchymal Stem Cells Restore the Ovary Function in an Ovariectomized Rat Model via an Antioxidant Effect Antioxidants (Basel) 2020-07-06 [PMID: 32640638] (WB, Rat)	WB	Rat
Kaya O, Orhan E, Sapmaz-Metin M, et al. The effects of epidermal growth factor on early burn-wound progression in rats Dermatol Ther 2019-12-18 [PMID: 31849151] (IF/IHC, Rat)	IF/IHC	Rat
Deng G, Ma C, Zhao H et al. Anti-edema and antioxidant combination therapy for ischemic stroke via glyburide-loaded betulinic acid nanoparticles Theranostics 2019-09-21 [PMID: 31660082] (WB, Human)	WB	Human
Tocmo R, Parkin K S-1-propenylmercaptocysteine protects murine hepatocytes against oxidative stress via persulfidation of Keap1 and activation of Nrf2 Free Radic. Biol. Med. 2019-07-23 [PMID: 31349040] (WB, Mouse)	WB	Mouse
Mossoba ME, Vohra S, Toomer H et al. Diglycolic acid induces HepG2/C3A liver cell toxicity in vitro Toxicol In Vitro. [PMID: 29890265] (Simple Western, Human)	Simple Western	Human
Dey S, Sayers CM, Verginadis II et al. ATF4-dependent induction of heme oxygenase 1 prevents anoikis and promotes metastasis. J Clin Invest 2015-07-01 [PMID: 26011642] (IF/IHC)	IF/IHC
Jang KJ, Mano H, Aoki K et al. Mitochondrial function provides instructive signals for activation-induced B-cell fates Nat Commun. Nat Commun 2015-04-10 [PMID: 25857523] (WB, Mouse) Details: Using the DyLight 650 conjugated version of NB600-302, catalog number NB600-302C.	WB	Mouse
Gupta A, Lacoste B, Pistel PJ et al. Neurotherapeutic effects of novel HO-1 inhibitors in vitro and in a transgenic mouse model of Alzheimer's disease J. Neurochem. 2014-08-11 [PMID: 25111043]
Kimbrel EA, Kouris NA, Yavanian G et al. Mesenchymal stem cell population derived from human pluripotent stem cells displays potent immunomodulatory and therapeutic properties. Stem Cells Dev 2014-03-20 [PMID: 24650034] (ICC/IF, Human) Details: Using the DyLight 488 conjugated version of NBP1-97507, catalog number NBP1-97507G.	ICC/IF	Human
FA Arosa et al. Red blood cells upregulate cytoprotective proteins and the labile iron pool in dividing human T cells despite a reduction in oxidative stress. Free Radic. Biol. Med. 35, 1404 . 2003-01-01 [PMID: 14642388] (WB, Human)	WB	Human
FR Jirik et al. Defective hematopoiesis and hepatic steatosis in mice with combined deficiencies of the genes encoding Fancc and Cu/Zn superoxide dismutase. Blood 98, 1003. 2001-01-01 [PMID: 11493445] (WB, Mouse)	WB	Mouse
DK Das et al. Hmox-1 constitutes an adaptive response to effect antioxidant cardioprotection: a study with transgenic mice heterozygous for targeted disruption of the Heme oxygenase-1 gene. Circulation 103, 1695. 2001-01-01 [PMID: 11273999] (WB, Mouse)	WB	Mouse
Show All 20 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Sitara Sankar	WB	Mouse	05/23/2017	View Summary Application Western Blot Sample Tested Mouse macrophages Species Mouse

Array NBP1-97507

• HO-1/HMOX1/HSP32 Antibodies
• HO-1/HMOX1/HSP32 ELISA Kit
• HO-1/HMOX1/HSP32 Lysate
• HO-1/HMOX1/HSP32 Proteins
• HO-1/HMOX1/HSP32 ELISA
• HO-1/HMOX1/HSP32 Proteins and Enzyme