![Western Blot: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - Western blot analysis using AMPK alpha 1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Azide-and-BSA-Free-Western-Blot-NBP2-80575-img0001.jpg "Western Blot: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - Western blot analysis using AMPK alpha 1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
| Applications | WB, Simple Western, ELISA, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | 2B7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human AMPK alpha 1 expressed in E. coli. [UniProt# Q13131] |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PRKAA1 |
| Purity | Ammonium sulfate precipitation |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | This AMPK alpha 1 (2B7) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:50. Separated by Size-Wes, Sally Sue/Peggy Sue. |
| Theoretical MW | 64 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Ammonium sulfate precipitation |
![Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg)
![Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16332.jpg)
![Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Flow_Cytometry_8283.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for AMPK alpha 1 Antibody (NBP2-80575)Find related products by research area.
|
|
AMPK Alpha 1 and lipid metabolism of adipocytes AMP-activated protein kinase (AMPK) is best known as a sensor of oxidative stress. AMPK is activated by increased intracellular AMP levels, which are a result of alterations in cellular metabolism from causes such as hypoxia, changes in ATP, sene... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PRKAA1 |
![Immunocytochemistry/Immunofluorescence: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - Immunofluorescence analysis of NTERA-2 cells using AMPK alpha 1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80575-img0002.jpg "Immunocytochemistry/Immunofluorescence: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - Immunofluorescence analysis of NTERA-2 cells using AMPK alpha 1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Image from the standard format of this antibody.")
![Immunohistochemistry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using AMPK alpha 1 mouse mAb with DAB staining. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80575-img0003.jpg "Immunohistochemistry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using AMPK alpha 1 mouse mAb with DAB staining. Image from the standard format of this antibody.")
![Flow Cytometry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - Flow cytometric analysis of PC-2 cells using AMPK alpha 1 mouse mAb (green) and negative control (purple). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80575-img0005.jpg "Flow Cytometry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - Flow cytometric analysis of PC-2 cells using AMPK alpha 1 mouse mAb (green) and negative control (purple). Image from the standard format of this antibody.")
![Flow Cytometry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - An intracellular stain was performed on RH-30 cells with AMPK alpha 1 Antibody (2B7) NBP2-22127PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80575-img0004.jpg "Flow Cytometry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - An intracellular stain was performed on RH-30 cells with AMPK alpha 1 Antibody (2B7) NBP2-22127PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Phycoerythrin.")
![ELISA: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Azide-and-BSA-Free-ELISA-NBP2-80575-img0006.jpg "ELISA: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng). Image from the standard format of this antibody.")
![Simple Western: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - Simple Western lane view shows a specific band for AMPK Alpha 1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Azide-and-BSA-Free-Simple-Western-NBP2-80575-img0007.jpg "Simple Western: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - Simple Western lane view shows a specific band for AMPK Alpha 1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.")
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![Immunohistochemistry-Paraffin TOR/mTOR [p Ser2448] Antibody - BSA Free](https://images.novusbio.com/images/TOR-mTOR-[p-Ser2448]-Antibody-Immunohistochemistry-Paraffin-NB600-607-img0005.jpg)
![Western Blot TOR/mTOR [p Ser2448] Antibody - BSA Free](https://images.novusbio.com/images/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NB600-607-img0006.jpg)
![Data TOR/mTOR [p Ser2448] Antibody - BSA Free](https://images.novusbio.com/images/TOR-mTOR-[p-Ser2448]-Antibody-N-A-NB600-607-img0008.jpg)
![N/A Adiponectin/Acrp30 [HRP]](https://images.novusbio.com/images2/DATA_Adiponectin_DRP300_ELISA_812.jpg)
![N/A Adiponectin/Acrp30 [HRP]](https://images.novusbio.com/images2/DATA_Adiponectin_DRP300_ELISA_813.jpg)
![N/A Adiponectin/Acrp30 [HRP]](https://images.novusbio.com/images2/Adiponectin_DRP300_ELISA_173.jpg)
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![N/A Leptin/OB [HRP]](https://images.novusbio.com/images2/Leptin_DLP00_ELISA_134.jpg)
![N/A Leptin/OB [HRP]](https://images.novusbio.com/images2/DATA_Leptin_DLP00_ELISA_749.jpg)
![Western Blot AMPK alpha 2 Antibody [Unconjugated]](https://images.novusbio.com/images2/AMPK_alpha_2_AF2850_Western_Blot_5219.jpg)
![Simple Western AMPK alpha 2 Antibody [Unconjugated]](https://images.novusbio.com/images2/AMPK_alpha_2_AF2850_Simple_Western_16678.jpg)
![Immunocytochemistry AMPK alpha 2 Antibody [Unconjugated]](https://images.novusbio.com/images2/AMPK_alpha_2_AF2850_Immunocytochemistry__Immunofluorescence_19686.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![Simple Western PYK2/FAK2 [p Tyr402] Antibody (592918) [Unconjugated]](https://images.novusbio.com/images2/PYK2_MAB6210_Simple_Western_16639.jpg)
![Western Blot PYK2/FAK2 [p Tyr402] Antibody (592918) [Unconjugated]](https://images.novusbio.com/images2/PYK2_MAB6210_Western_Blot_9430.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")