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AMPK alpha 1 Antibody (2B7) [PE/Cy7]

 
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Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC
Clone
2B7
Clonality
Monoclonal
Host
Mouse
Conjugate
PE/Cy7

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-22127AF350
Alexa Fluor 405
NBP2-22127AF405
Alexa Fluor 488
NBP2-22127AF488
Alexa Fluor 532
NBP2-22127AF532
Alexa Fluor 594
NBP2-22127AF594
Alexa Fluor 647
NBP2-22127AF647
Alexa Fluor 700
NBP2-22127AF700
Alexa Fluor 750
NBP2-22127AF750
Allophycocyanin
NBP2-22127APC
Allophycocyanin/Cy7
NBP2-22127APCCY7
Biotin
NBP2-22127B
DyLight 350
NBP2-22127UV
DyLight 405
NBP2-22127V
DyLight 488
NBP2-22127G
DyLight 550
NBP2-22127R
DyLight 594
NBP2-22127DL594
DyLight 650
NBP2-22127C
DyLight 680
NBP2-22127FR
DyLight 755
NBP2-22127IR
FITC
NBP2-22127F
HRP
NBP2-22127H
Janelia Fluor 549
NBP2-22127JF549
Janelia Fluor 646
NBP2-22127JF646
PE
NBP2-22127PE
PE/Atto594
NBP2-22127PEATT594
PE/Cy5.5
NBP2-22127PECY55
PerCP
NBP2-22127PCP
Unconjugated
NBP2-22127
mFluor Violet 450 SE
NBP2-22127MFV450
mFluor Violet 500 SE
NBP2-22127MFV500
mFluor Violet 610 SE
NBP2-22127MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-22127
BSA Free
NBP2-22127
BSA Free
NBP2-22127SS
Azide and BSA Free
NBP2-80575
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AMPK alpha 1 Antibody (2B7) [PE/Cy7] Summary

Immunogen
Purified recombinant fragment of human AMPK alpha 1 expressed in E. coli. [UniProt# Q13131]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRKAA1
Purity
Ammonium sulfate precipitation
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Ammonium sulfate precipitation

Alternate Names for AMPK alpha 1 Antibody (2B7) [PE/Cy7]

  • AMP -activate kinase alpha 1 subunit
  • AMP-activated protein kinase, catalytic, alpha-1,5'-AMP-activated protein kinase catalytic subunit alpha-1
  • AMPK alpha 1
  • AMPK alpha 1,5'-AMP-activated protein kinase, catalytic alpha-1 chain
  • AMPK subunit alpha-1
  • AMPK
  • AMPK1
  • AMPKa1
  • EC 2.7.11
  • EC 2.7.11.1
  • MGC33776
  • MGC57364
  • PRKAA1
  • protein kinase, AMP-activated, alpha 1 catalytic subunit

Background

The 5'-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non-fermentor) kinase family (1) AMPK is a heterotrimeric protein comprising alpha (63 kDa), beta (38 kDa) and gamma (38 kDa) subunits. The alpha subunit is the catalytic subunit (which requires phosphorylation to be active) (2-3). Beta and gamma are non-catalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (4). AMPK also appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PRKAA1