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Human Leptin Quantikine ELISA Kit

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Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Human Leptin Quantikine ELISA Kit Summary

Background
The Quantikine Human Leptin Immunoassay is a 3.5 hour solid phase ELISA designed to measure soluble human Leptin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Leptin and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant Leptin accurately. Results obtained measuring natural huma...n Leptin showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Leptin.
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Specificity
Natural and recombinant human Leptin
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
LEP

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Interference observed with 1 or more available related molecules.
Publications
Read Publications using DLP00.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Leptin Quantikine ELISA Kit

  • FLJ94114
  • LEP
  • leptin (murine obesity homolog)
  • leptin (obesity homolog, mouse)
  • Leptin
  • OB
  • Obese protein
  • obese, mouse, homolog of
  • Obesity factor
  • OBOBS

Background

Leptin is a product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin are obese and diabetic, and have reduced activity, metabolism, and body temperature. Rat leptin shares approximately 96% and 82% sequence identity with the mouse and human protein, respectively. The expression of leptin mRNA is restricted to adipose tissue.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Leptin/OB (DLP00)(151)

We have publications tested in 3 confirmed species: Human, Mouse,  Human.


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Human
(149)
Mouse
(1)
 Human
(1)
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Showing Publications 1 - 10 of 151. Show All 151 Publications.
Publications using DLP00 Applications Species
Ma?uj?o-Balcerska, E;Pietras, T; Adipocytokines levels as potential biomarkers for discriminating patients with a diagnosis of depressive disorder from healthy controls Journal of psychiatric research 2024-01-20 [PMID: 38290234] (Human) Human
Hernandez, JD;Li, T;Ghannam, H;Rau, CM;Masuda, MY;Madura, JA;Jacobsen, EA;Filippis, E; Linking adipose tissue eosinophils, interleukin-4, and leptin in human obesity and insulin resistance JCI insight 2024-01-11 [PMID: 38206766] (Human) Human
Barril, S;Acebo, P;Millan-Billi, P;Luque, A;Sibila, O;Tarín, C;Tazi, A;Castillo, D;Hortelano, S; Bronchoalveolar cytokine profile differentiates Pulmonary Langerhans cell histiocytosis patients from other smoking-related interstitial lung diseases Respiratory research 2023-12-18 [PMID: 38111019] (Human) Human
Bilska, K;Dmitrzak-W?glarz, M;Osip, P;Pawlak, J;Paszy?ska, E;Permoda-Pachuta, A; Metabolic Syndrome and Adipokines Profile in Bipolar Depression Nutrients 2023-10-25 [PMID: 37960185] (Human) Human
Nederveen, JP;Mastrolonardo, AJ;Xhuti, D;Di Carlo, A;Manta, K;Fuda, MR;Tarnopolsky, MA; Novel Multi-Ingredient Supplement Facilitates Weight Loss and Improves Body Composition in Overweight and Obese Individuals: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial Nutrients 2023-08-23 [PMID: 37686725] (Human) Human
Sparks, L;Whytock, K;Divoux, A;Sun, Y;Pino, M;Yu, G;Smith, S;Walsh, M; A single nuclei atlas of aging human abdominal subcutaneous white adipose tissue Research square 2023-07-13 [PMID: 37503028] (Human) Human
Zhao, Z;Huang, J;Zhong, D;Wang, Y;Che, Z;Xu, Y;Hong, R;Qian, Y;Meng, Q;Yin, J; Associations of three thermogenic adipokines with metabolic syndrome in obese and non-obese populations from the China plateau: the China Multi-Ethnic Cohort BMJ open 2023-07-25 [PMID: 37491087] (Human) Human
Catalán-Lambán, A;Ojeda-Rodríguez, A;Marti Del Moral, A;Azcona-Sanjulian, C; Changes in objectively measured sleep after a multidisciplinary lifestyle intervention in children with abdominal obesity: A randomized trial Sleep medicine 2023-07-17 [PMID: 37487278] ( Human)  Human
Pletikosic, I;Marasovic Krstulovic, D;Bakovic, D;Susilovic Grabovac, Z;Tandara, L;Martinovic Kaliterna, D; Association of inflammatory biomarkers and disease activity with subclinical myocardial dysfunction in psoriatic arthritis Scientific reports 2023-06-26 [PMID: 37365233] (Human) Human
R Hambly, N Kearney, R Hughes, JM Fletcher, B Kirby Metformin Treatment of Hidradenitis Suppurativa: Effect on Metabolic Parameters, Inflammation, Cardiovascular Risk Biomarkers, and Immune Mediators International Journal of Molecular Sciences, 2023-04-09;24(8):. 2023-04-09 [PMID: 37108132] (Human) Human
Show All 151 Publications.

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Bioinformatics

Gene Symbol LEP