We are interested in your item, NB400-114. Could you please provide us specific protocol of NB400-114 for IHC-P?

Name: Fatty Acid Synthase/FASN Antibody
Brand: Novus Biologicals
SKU: NB400-114
Rating: 4 (2 reviews)

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If you were requesting the protocol for WB, then we have a special one for this antibody because it is highly acetylated and tightly associated with membrane. However, we don't have any special IHC-P protocol for NB400-114, and the general one can be used: view protocol. (If there was a special IHC-P protocol for this antibody, it would have posted on the webpage for this product; but it does not) The only thing you will concern for using this general IHC-P would be how to do antigen retrieval. Human Protein Atlas shows that for performing antigen retrieval during IHC-P experiments ProteinAtlas site, HIER pH 6 can be applied. For this, both human and mouse tissues are applicable to HIER pH 6.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, Po, Ch, Fe, Ha, Pl, PmSpecies Glossary
Applications	WB, ICC/IF, IHC, IP, KD
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated

Immunogen	A synthetic peptide, conjugated to KLH, made near the N-terminus of mouse FAS. [Swiss-Prot# P19096]
Localization	Cytoplasmic
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	FASN
Purity	Unpurified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer	Whole antisera
Preservative	0.05% Sodium Azide
Purity	Unpurified

Publications using NB400-114	Applications	Species
Gupta K, Mukherjee S, Sen S, Sonawane M Coordinated activities of Myosin Vb isoforms and mTOR signaling regulate epithelial cell morphology during development Development (Cambridge, England) 2022-03-15 [PMID: 35299238]
Han Y, Lee Y, Jang Y et Al. Aspirin Improves Nonalcoholic Fatty Liver Disease and Atherosclerosis through Regulation of the PPARdelta-AMPK-PGC-1 alpha Pathway in Dyslipidemic Conditions BioMed Research International 2020-03-19 [PMID: 32258142] (WB, Mouse, Human)	WB	Mouse, Human
Iizuka Y, Chiba K, Kim H et al. Impact of discontinuation of fish oil after pioglitazone-fish oil combination therapy in diabetic KK mice J. Nutr. Biochem. 2019-11-07 [PMID: 31760227] (WB, Mouse)	WB	Mouse
Gang, X;Xuan, L;Zhao, X;Lv, Y;Li, F;Wang, Y;Wang, G; Speckle-type POZ protein suppresses lipid accumulation and prostate cancer growth by stabilizing fatty acid synthase Prostate 2019-04-07 [PMID: 30955223] (WB, Human)	WB	Human
Li MD, Vera NB, Yang Y et al. Adipocyte OGT governs diet-induced hyperphagia and obesity Nat Commun 2018-11-30 [PMID: 30504766] (WB, Mouse)	WB	Mouse
Walz JZ, Saha J, Arora A et al. Fatty acid synthase as a potential therapeutic target in feline oral squamous cell carcinoma Vet Comp Oncol 2017-09-04 [PMID: 28871635] (WB, Feline)	WB	Feline
Park SH, Sung YY, Jang S et al. The Korean herbal medicine, Do In Seung Gi-Tang, attenuates atherosclerosis via AMPK in high-fat diet-induced ApoE(-/-) mice. BMC Complement Altern Med. 2016-09-08 [PMID: 27608856] (IF/IHC, Mouse)	IF/IHC	Mouse
Chen CC, Chuang WT, Lin AH et al. Andrographolide inhibits adipogenesis of 3T3-L1 cells by suppressing C/EBPbeta expression and activation. Toxicol. Appl. Pharmacol. 2016-09-15 [PMID: 27475717] (ICC/IF, Mouse)	ICC/IF	Mouse
Karlas A, Berre S, Couderc T et al. A human genome-wide loss-of-function screen identifies effective chikungunya antiviral drugs. Nat Commun. 2016-05-14 [PMID: 27177310] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Gang X, Yang Y, Zhong J et al. P300 acetyltransferase regulates fatty acid synthase expression, lipid metabolism and prostate cancer growth. Oncotarget 2016-03-22 [PMID: 26934656] (WB)	WB
Kim K, Cho YM, Park BH et al. Histological and immunohistochemical markers for progression prediction in transurethrally resected high-grade non-muscle invasive bladder cancer Int J Clin Exp Pathol. 2015-01-01 [PMID: 25755769] (IHC-P, Human) Details: Fatty Acid Synthase/FASN antibody used for IHC-P on formalin-fixed paraffin-embedded TMA sections that were creataed from human bladder cancer tissue blocks of TURBT specimens. The primary antibody was used at 1:500 dilution and the staining protocol involved automated system BenchMark XT from Ventana Medical Systems and an ultraView Universal DAB detection kit. FASN was found to localize to the cytoplasm of tumor cells and a representative image is shown in Figure 1F.	IHC-P	Human
Eser PO, Vanden Heuvel JP, Araujo J et al. Marine- and plant-derived omega-3 fatty acids differentially regulate prostate cancer cell proliferation. Mol Clin Oncol 2013-05-01 [PMID: 24649190] (Plant)		Plant
He Y, Li Y, Zhao T et al. Ursolic Acid Inhibits Adipogenesis in 3T3-L1 Adipocytes through LKB1/AMPK Pathway. PLoS One 2013-07-26 [PMID: 23922935] (WB, Mouse)	WB	Mouse
Zhang Wencheng, Wang Qilong, Song Ping, Zou Ming-Hui. Liver kinase b1 is required for white adipose tissue growth and differentiation. Diabetes 2013-01-01 [PMID: 23396401] (WB, Mouse)	WB	Mouse
Grenier E, Garofalo C, Delvin E, Levy E. Modulatory Role of PYY in Transport and Metabolism of Cholesterol in Intestinal Epithelial Cells plos One 2012-01-01 [PMID: 22844422] (WB, Human)	WB	Human
Oliva J, French SW, Li J et al. Proteasome inhibitor treatment reduced fatty acid, triacylglycerol and cholesterol synthesis Experimental and Molecular Pathology 2012-08-01 [PMID: 22445925] (WB, Rat)	WB	Rat
Chow JD, Jones ME, Prelle K, Simpson ER, Boon WC. A selective estrogen receptor ? agonist ameliorates hepatic steatosis in the male aromatase knockout mouse. J;210(3):323-34. 2011-09-01 [PMID: 21705395] (WB, Mouse)	WB	Mouse
Basciano H et al. Metabolic effects of dietary cholesterol in an animal model of insulin resistance and hepatic steatosis. Am J Physiol Endocrinol Metab;297(2):E462-73. 2009-08-01 [PMID: 19509184] (WB)	WB
Basciano H, Miller A, Baker C et al. LXR{alpha} activation perturbs hepatic insulin signaling stimulates production of apolipoprotein B-containing lipoproteins. Am J Physiol Gastrointest Liver Physiol;297(2):G323-332. 2009-01-01 [PMID: 19497957] (WB, Hamster)	WB	Hamster
Bryzgalova G, Lundholm L, Portwood N,et al. Mechanisms of antidiabetogenic and body weight-lowering effects of estrogen in high-fat diet-fed mice. Am J Physiol Endocrinol Metab;295(4):E904-12. 2008-10-01 [PMID: 18697913]
Vavere, AL et al. 1-11C-acetate as a PET radiopharmaceutical for imaging fatty acid synthase expression in prostate cancer. J Nucl Med;49(2):327-34. 2008-02-01 [PMID: 18199615]
Heemers, HV et al. Androgen deprivation increases p300 expression in prostate cancer cells. Cancer Res;67(7):3422-30. 2007-04-01 [PMID: 17409453]
Gao H, Bryzgalova G, Hedman E et al. Long-term administration of estradiol decreases expression of hepatic lipogenic genes and improves insulin sensitivity in ob/ob mice: a possible mechanism is through direct regulation of signal transducer and activator of transcription 3. Mol Endocrinol;20(6):1287-99. 2006-06-01 [PMID: 16627594]
Dridi S et al. FAS inhibitor cerulenin reduces food intake and melanocortin receptor gene expression without modulating the other (an)orexigenic neuropeptides in chickens. Am J Physiol Regul Integr Comp Physiol;291(1):R138-47. 2006-07-01 [PMID: 16455759] (WB, IHC-P, Chicken)	WB, IHC-P	Chicken
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Fatty Acid Synthase/FASN Antibody

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Gene Symbol	FASN
Entrez	2194 (Human) 14104 (Mouse)
Uniprot	P19096(Mouse)

	Kristine Griffett 03/12/2013
Application:	IF
Species:	Mouse

	Verified Customer 12/06/2011
Application:	WB
Species:	Rat