![Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Expression of Fatty Acid Synthase in chicken hypothalamus. A: total RNA (1 ug) isolated from different tissues (brain, hypothalamus, liver and muscle) was subjected to RT-PCR using specific primers for chicken Fatty Acid Synthase (GenBank accession JO4485) or ribosomal 18S as a control (GenBank accession AF173612) B: tissue lysates (brain, hypothalmus, liver and muscle) were subjected to Western blot as described in MATERIALS AND METHODS. Blots were incubated with anti-Fatty Acid Synthase antibody and revealed by enhanced chemiluminescence. Picture compliments of Dridi S. et al, Am J Physiol Regul Integr Comp Physiol. 2006 Jul;291(1):R138-47. Epub 2006 Feb 2.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Western-Blot-NB400-114-img0011.jpg "Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Expression of Fatty Acid Synthase in chicken hypothalamus. A: total RNA (1 ug) isolated from different tissues (brain, hypothalamus, liver and muscle) was subjected to RT-PCR using specific primers for chicken Fatty Acid Synthase (GenBank accession JO4485) or ribosomal 18S as a control (GenBank accession AF173612) B: tissue lysates (brain, hypothalmus, liver and muscle) were subjected to Western blot as described in MATERIALS AND METHODS. Blots were incubated with anti-Fatty Acid Synthase antibody and revealed by enhanced chemiluminescence. Picture compliments of Dridi S. et al, Am J Physiol Regul Integr Comp Physiol. 2006 Jul;291(1):R138-47. Epub 2006 Feb 2.")
| Reactivity | Hu, Mu, Rt, Po, Ch, Fe, Ha, Pl, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | A synthetic peptide, conjugated to KLH, made near the N-terminus of mouse FAS. [Swiss-Prot# P19096] |
| Localization | Cytoplasmic |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | FASN |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||
| Application Notes | This Fatty Acid Synthase antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation and Western Blot where a band at ~272 kDa is observed. May see 1 or 2 minor cross-reacting lower MW bands in liver tissue. In ICC/IF cytoplasmic staining can be seen in MCF7 cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
||
| Theoretical MW | 272 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
||
| Control |
|
||
| Reviewed Applications |
|
||
| Publications |
|
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | Whole antisera |
| Preservative | 0.05% Sodium Azide |
| Purity | Unpurified |
![Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg)
![Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16332.jpg)
![Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Flow_Cytometry_8283.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
|
reviewed by:
Kristine Griffett |
IF | Mouse | 03/12/2013 |
Summary
|
|||||||
|
reviewed by:
Verified Customer |
WB | Rat | 12/06/2011 |
Summary
|
Secondary Antibodies |
Isotype Controls |
Research Areas for Fatty Acid Synthase/FASN Antibody (NB400-114)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
5 | |
4 | |
3 | |
2 | |
1 |
| Kristine Griffett 03/12/2013 |
||
| Application: | IF | |
| Species: | Mouse |
| Verified Customer 12/06/2011 |
||
| Application: | WB | |
| Species: | Rat |
![Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NB400-114] - The Fas antibody was tested in MCF-7 cells at a 1:2000 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Immunocytochemistry-Immunofluorescence-NB400-114-img0010.jpg "Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NB400-114] - The Fas antibody was tested in MCF-7 cells at a 1:2000 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.")
![Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NB400-114] - Immunohistochemical localization of FAS in chicken hypothalmus. Paraffin sections were obtained from 3-wk-old broiler chickens.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Immunohistochemistry-Paraffin-NB400-114-img0009.jpg "Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NB400-114] - Immunohistochemical localization of FAS in chicken hypothalmus. Paraffin sections were obtained from 3-wk-old broiler chickens.")
![Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Analysis of Fatty Acid Synthase, using NB400-114. Samples: 50 ug of total mouse liver lysate.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Western-Blot-NB400-114-img0007.jpg "Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Analysis of Fatty Acid Synthase, using NB400-114. Samples: 50 ug of total mouse liver lysate.")
![Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NB400-114] - Fatty acid synthesis requirement for CHIKV life cycle. Confocal section of CHIKV replicon-infected HeLa cells labelled for FASN, dsRNA and 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms11320), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Immunocytochemistry-Immunofluorescence-NB400-114-img0012.jpg "Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NB400-114] - Fatty acid synthesis requirement for CHIKV life cycle. Confocal section of CHIKV replicon-infected HeLa cells labelled for FASN, dsRNA and 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms11320), licensed under a CC-BY license.")
![Knockdown Validated: Fatty Acid Synthase/FASN Antibody [NB400-114] - Fatty acid synthesis requirement for CHIKV life cycle. Western blot showing silencing efficiency of FASN-, ACC- and ACLY-specific siRNAs on CHIKV replication (n=10 for each data set).](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Knockdown-Validated-NB400-114-img0013.jpg "Knockdown Validated: Fatty Acid Synthase/FASN Antibody [NB400-114] - Fatty acid synthesis requirement for CHIKV life cycle. Western blot showing silencing efficiency of FASN-, ACC- and ACLY-specific siRNAs on CHIKV replication (n=10 for each data set).")
![Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Fatty acid synthesis requirement for CHIKV life cycle.(a) Impact of FASN or ACLY knockdown on CHIKV replication. Closed & open symbols indicate replicates from the primary screen & during validation, respectively. (b) Western blot showing silencing efficiency of siRNAs used in c. (c) Impact of FASN-, ACC- & ACLY-specific siRNAs on CHIKV replication (n=10 for each data set). (d) Confocal section of CHIKV replicon-infected HeLa cells labelled for FASN, dsRNA & 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 10 μm. (e) Co-localization analysis of cells labelled as in d & in Supplementary Fig. 3b, plotted as Pearson's coefficient per cell. Each symbol corresponds to a cell stack from three independent experiments (n=29 cells for FASN, 30 cells for ACC & 31 cells for ACLY); median values shown in red. (f) Effect of FASN (cerulenin, n=12 for each data set), ACC (TOFA, n=11 for each data set) & ACLY (BMS-303141 n=11 for each data set) inhibitors on CHIKV replication. (g) Real-time cell toxicity assay performed on HeLa cells (n=3 for each point). Excepted for b & d where representative images are shown & for g where the mean±s.d. is shown for each point of a representative experiment, all data represent the means±s.e.m. of three independent experiments analysed using one-way analysis of variance with Tukey's post test (*P<0.05; **P<0.01; ***P<0.001; NSP≥0.05). NS, not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27177310), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-114_rabbit-polyclonal-fatty-acid-synthase-fasn-antibody-31020241618926.jpg "Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Fatty acid synthesis requirement for CHIKV life cycle.(a) Impact of FASN or ACLY knockdown on CHIKV replication. Closed & open symbols indicate replicates from the primary screen & during validation, respectively. (b) Western blot showing silencing efficiency of siRNAs used in c. (c) Impact of FASN-, ACC- & ACLY-specific siRNAs on CHIKV replication (n=10 for each data set). (d) Confocal section of CHIKV replicon-infected HeLa cells labelled for FASN, dsRNA & 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 10 μm. (e) Co-localization analysis of cells labelled as in d & in Supplementary Fig. 3b, plotted as Pearson's coefficient per cell. Each symbol corresponds to a cell stack from three independent experiments (n=29 cells for FASN, 30 cells for ACC & 31 cells for ACLY); median values shown in red. (f) Effect of FASN (cerulenin, n=12 for each data set), ACC (TOFA, n=11 for each data set) & ACLY (BMS-303141 n=11 for each data set) inhibitors on CHIKV replication. (g) Real-time cell toxicity assay performed on HeLa cells (n=3 for each point). Excepted for b & d where representative images are shown & for g where the mean±s.d. is shown for each point of a representative experiment, all data represent the means±s.e.m. of three independent experiments analysed using one-way analysis of variance with Tukey's post test (*P<0.05; **P<0.01; ***P<0.001; NSP≥0.05). NS, not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27177310), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Immunohistochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Immunohistochemistry_21186.jpg)
![Western Blot Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Western_Blot_11656.jpg)
![Immunocytochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Immunocytochemistry_11911.jpg)
![N/A Leptin/OB [HRP]](https://images.novusbio.com/images2/Leptin_DLP00_ELISA_134.jpg)
![N/A Leptin/OB [HRP]](https://images.novusbio.com/images2/DATA_Leptin_DLP00_ELISA_749.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![Western Blot SCD-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/SCD-1_AF7550_Western_Blot_12344.jpg)
![Knockout Validated SCD-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/SCD1_AF7550_Knockout_Validated_24050.jpg)
![Knockout Validated SCD-1 Antibody [Unconjugated]](https://images.novusbio.com/images/af7550_human-scd-1-affinity-purified-polyclonal-ab-20820241518560.png)
![SDS-PAGE ErbB2/Her2 [Unconjugated]](https://images.novusbio.com/images2/ErbB2_1129-ER_70.jpg)
![Bioactivity ErbB2/Her2 [Unconjugated]](https://images.novusbio.com/images2/1129-er_recombinant-human-erbb2-her2-fc-chimera-protein-cf-bioactivity-7122020142841.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")