AMPK alpha 1 Antibodies
We offer AMPK alpha 1 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunoprecipitation (Negative), Sandwich ELISA, Simple Western, Western Blot. Each AMPK alpha 1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our AMPK alpha 1 Antibodies can be used in a variety of model species: Bovine, Canine, Chicken, Human, Insect, Invertebrate, Mouse, Primate, Rat. Use the list below to choose the AMPK alpha 1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our AMPK alpha 1 polyclonal antibodies and browse our AMPK alpha 1 monoclonal antibody catalog.
Alternate Names for AMPK alpha 1 Antibodies
anti-AMPK alpha 1 antibody, anti-PRKAA1 antibody, anti-AMP -activate kinase alpha 1 subunit antibody, anti-AMP-activated protein kinase, catalytic, alpha-1,5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody, anti-AMPK alpha 1,5'-AMP-activated protein kinase, catalytic alpha-1 chain antibody, anti-AMPK antibody, anti-AMPK subunit alpha-1 antibody, anti-AMPK1 antibody, anti-AMPKa1 antibody, anti-EC 2.7.11 antibody, anti-EC 2.7.11.1 antibody, anti-MGC33776 antibody, anti-MGC57364 antibody, anti-protein kinase, AMP-activated, alpha 1 catalytic subunit antibody
13 Results for "ampk-alpha-1" in Primary Antibodies
![Simple Western: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Simple Western lane view shows a specific band for AMPK Alpha 1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Simple-Western-NBP2-22127-img0006.jpg "Simple Western: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Simple Western lane view shows a specific band for AMPK Alpha 1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Western blot analysis using AMPK alpha 1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Western-Blot-NBP2-22127-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Immunofluorescence analysis of NTERA-2 cells using AMPK alpha 1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Immunocytochemistry-Immunofluorescence-NBP2-22127-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Ability to combat ROS varies across cell lines. Western blot analysis showing levels of AMPK and activated AMPK (T172) at 24, 48 and 72 hours following exposure to 0.4 uM GNE-617. Full-length western blots are shown in S2D Fig. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0164166), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Western-Blot-NBP2-22127-img0009.jpg)
![Immunohistochemistry-Paraffin: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using AMPK alpha 1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Immunohistochemistry-Paraffin-NBP2-22127-img0005.jpg)
![Flow Cytometry: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - An intracellular stain was performed on NIH3T3 cells with AMPK alpha 1 Antibody (2B7) NBP2-22127 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Flow-Cytometry-NBP2-22127-img0008.jpg)
![Flow Cytometry: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - An intracellular stain was performed on RH-30 cells with AMPK alpha 1 Antibody (2B7) NBP2-22127PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Flow-Cytometry-NBP2-22127-img0007.jpg)
![ELISA: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-ELISA-NBP2-22127-img0001.jpg)
![Western Blot: AMPK alpha 1 Antibody [NB100-239] - Detection of human and mouse AMPK alpha 1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AMPK alpha 1 antibody NB100-239 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-Western-Blot-NB100-239-img0006.jpg "Western Blot: AMPK alpha 1 Antibody [NB100-239] - Detection of human and mouse AMPK alpha 1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AMPK alpha 1 antibody NB100-239 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Immunohistochemistry: AMPK alpha 1 Antibody [NB100-239] - Detection of human AMPK alpha 1 by immunohistochemistry. Sample: FFPE section of human smooth muscle. Antibody: Affinity purified rabbit anti-AMPK alpha 1 (NB100-239). Detection: DAB](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-Immunohistochemistry-NB100-239-img0005.jpg)
![Immunoprecipitation: AMPK alpha 1 Antibody [NB100-239] - Detection of human AMPK alpha 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AMPK alpha 1 antibody NB100-239 (lot 2) used for IP at 3 ug per reaction. AMPK alpha 1 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated AMPK alpha 1, NB100-239 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-Immunoprecipitation-NB100-239-img0007.jpg)
![Western Blot: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - Immunoblot analysis of total cell extracts from MIN6 cells grown in the medium containing indicated glucose using AMPK alpha 1 (Phospho-Ser496) Antibody. This image was submitted via customer review.](http://images.novusbio.com/fullsize/AMPK-alpha-1-[p-Ser496]-Antibody-Western-Blot-NB100-81784-img0016.jpg "Western Blot: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - Immunoblot analysis of total cell extracts from MIN6 cells grown in the medium containing indicated glucose using AMPK alpha 1 (Phospho-Ser496) Antibody. This image was submitted via customer review.")
![Immunocytochemistry/Immunofluorescence: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - Staining of methanol-fixed Hela cells using NB100-81784](http://images.novusbio.com/fullsize/AMPK-alpha-1-[p-Ser496]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-81784-img0011.jpg)
![Immunohistochemistry-Paraffin: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - Paraffin-embedded human colon carcinoma tissue using NB100-81784 (left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/AMPK-alpha-1-[p-Ser496]-Antibody-Immunohistochemistry-Paraffin-NB100-81784-img0013.jpg)
![Western Blot: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - analysis of extracts from C6 cells, treated with EGF or calf intestinal phosphatase (CIP), using AMPK alpha1 (Phospho-Ser496) Antibody.](http://images.novusbio.com/fullsize/AMPK-alpha-1-[p-Ser496]-Antibody-Western-Blot-NB100-81784-img0009.jpg)
![Western Blot: AMPK alpha 1 [p Ser496] Antibody [NB100-81784] - Analysis of extracts from HUVEC cells untreated or treated with UV using AMPK alpha 1(Phospho-Ser496)Antibody](http://images.novusbio.com/fullsize/AMPK-alpha-1-[p-Ser496]-Antibody-Western-Blot-NB100-81784-img0015.jpg)
![Western Blot: AMPK alpha 1 Antibody [NBP2-49430] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-Western-Blot-NBP2-49430-img0002.jpg "Western Blot: AMPK alpha 1 Antibody [NBP2-49430] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG")
![Immunohistochemistry: AMPK alpha 1 Antibody [NBP2-49430] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-Immunohistochemistry-NBP2-49430-img0001.jpg)
![Western Blot: AMPK alpha 1 Antibody (RM301) [NBP2-61491] - Western Blot of HeLa cells lysate using NBP2-61491 at a 1:100 dilution](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-RM301-Western-Blot-NBP2-61491-img0005.jpg "Western Blot: AMPK alpha 1 Antibody (RM301) [NBP2-61491] - Western Blot of HeLa cells lysate using NBP2-61491 at a 1:100 dilution")
![Immunohistochemistry: AMPK alpha 1 Antibody (RM301) [NBP2-61491] - Immunohistochemical staining of formalin fixed and paraffin embedded human colon tissue section using NBP2-61491 at a 1:200 dilution](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-RM301-Immunohistochemistry-NBP2-61491-img0004.jpg)
![Western Blot: AMPK alpha 1 [p Ser496] Antibody (S01-3B7) [NBP3-19825] - Western blot detection of AMPK alpha 1 (phospho-Ser496) in Ramos cell lysates using NBP3-19825 (1:1000 diluted). Predicted band size: 64kDa. Observed band size: 64kDa.](http://images.novusbio.com/fullsize/AMPK-alpha-1-p-Ser496-Antibody-S01-3B7-Western-Blot-NBP3-19825-img0002.jpg "Western Blot: AMPK alpha 1 [p Ser496] Antibody (S01-3B7) [NBP3-19825] - Western blot detection of AMPK alpha 1 (phospho-Ser496) in Ramos cell lysates using NBP3-19825 (1:1000 diluted). Predicted band size: 64kDa. Observed band size: 64kDa.")
![Immunocytochemistry/Immunofluorescence: AMPK alpha 1 [p Ser496] Antibody (S01-3B7) [NBP3-19825] - Immunocytochemistry of AMPK alpha 1 (phospho-Ser496) (green) in hela using NBP3-19825 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/AMPK-alpha-1-p-Ser496-Antibody-S01-3B7-Immunocytochemistry-Immunofluorescence-NBP3-19825-img0001.jpg)
![Western Blot: AMPK alpha 1 [p Ser485] Antibody [NBP3-05462] - Western blot analysis of extracts of 293T cells, using AMPK alpha 1 antibody (NBP3-05462) at 1:1000 dilution.293T cells were treated by oligomycin (0.5 uM) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/AMPK-alpha-1-[p-Ser485]-Antibody-Western-Blot-NBP3-05462-img0001.jpg "Western Blot: AMPK alpha 1 [p Ser485] Antibody [NBP3-05462] - Western blot analysis of extracts of 293T cells, using AMPK alpha 1 antibody (NBP3-05462) at 1:1000 dilution.293T cells were treated by oligomycin (0.5 uM) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: AMPK alpha 1 [p Ser 496] Antibody (SR2088) [NBP3-21561] - Western blot analysis using NBP3-21561 in (1) 293T cell lysate treated with LP; (2) 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-21561_rabbit-ampk-alpha-1-p-ser-496-mab-sr2088-5720231456543.jpg "Western Blot: AMPK alpha 1 [p Ser 496] Antibody (SR2088) [NBP3-21561] - Western blot analysis using NBP3-21561 in (1) 293T cell lysate treated with LP; (2) 293T cell lysate.")
![Western Blot: AMPK alpha 1 Antibody (1G4) [H00005562-M01] - PRKAA1 monoclonal antibody (M01), clone 1G4 Analysis of PRKAA1 expression in HepG2.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-1G4-Western-Blot-H00005562-M01-img0004.jpg "Western Blot: AMPK alpha 1 Antibody (1G4) [H00005562-M01] - PRKAA1 monoclonal antibody (M01), clone 1G4 Analysis of PRKAA1 expression in HepG2.")
![ELISA: AMPK alpha 1 Antibody (1G4) [H00005562-M01] - Detection limit for recombinant GST tagged PRKAA1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-1G4-ELISA-H00005562-M01-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: AMPK alpha 1 Antibody [NBP2-58302] - Staining of human cell line U-251 MG shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58302-img0001.jpg "Immunocytochemistry/Immunofluorescence: AMPK alpha 1 Antibody [NBP2-58302] - Staining of human cell line U-251 MG shows localization to nuclear speckles.")