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PPAR gamma/NR1C3 Antibody - BSA Free

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Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

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Reviews & Publications
Protocols & FAQs
Support & Research

PPAR gamma/NR1C3 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal portion of the human PPAR gamma protein (between residues 20-120) [UniProt P37231]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPARG
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation reported in scientific literature (PMID 27862692)
  • Western Blot 2 ug/ml
Application Notes
In Western blot, a band is detected at ~55 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Adrenal Whole Tissue Lysate (Adult Whole Normal)
Human Adipose Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 2 Reviews rated 4
using
NBP2-22106 in the following applications:

Publications
Read Publications using
NBP2-22106 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27862692).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PPAR gamma/NR1C3 Antibody - BSA Free

  • CIMT1
  • NR1C3
  • NR1C3GLM1
  • Nuclear receptor subfamily 1 group C member 3
  • peroxisome proliferator-activated receptor gamma 1
  • peroxisome proliferator-activated receptor gamma
  • PPAR gamma
  • PPARG
  • PPARG1peroxisome proliferative activated receptor, gamma
  • PPARG2PPARgamma
  • PPAR-gamma

Background

Peroxisome proliferator-activated receptor gamma (PPARG), also known as nuclear receptor subfamily 1 group C member 3, NR1C3, GLM1, CIMT1, PPARG1, PPARG2, and PPARgamma, regulates adipocyte differentiation and binds peroxisome proliferators that include hypolipidemic drugs and fatty acids. PPARG regulates the peroxisomal beta-oxidation pathway of these fatty acids. PPARG activates transcription for acyl-CoA oxidase and regulates glucose homeostasis. PPARG is a member of the PPAR family, which is a subfamily of nuclear receptors. PPARG is found in the nucleus and the cytoplasm and is most prevalent in adipose tissue but also found in skeletal muscle, spleen, heart, liver, placenta, lung, and ovary. PPARG is found in three known isoforms (57.6, 54.8, and 21.6 kDa), which are produced by alternative splicing. The PPARG protein is subject to phosphorylation. PPARG has been linked to many diseases such as obesity, diabetes, atherosclerosis, and cancer. Recent research has indicated that mutations in PPARG play a role in non-alcoholic fatty liver disease (PMID: 22160635). Other research has found that using PPARG ligands as part of a chemotherapy treatment could help improve treatment for advanced pancreatic cancer (PMID: 22020928). Similar cancer research discovered that PPARG can be used to induce colorectal carcinoma cell death, making PPARG, in conjunction with survivin and caspase-3, a potential cancer cell regulator (PMID: 21765467).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Recent Reviews

4
5
1
4
0
3
1
2
0
1
0

Verified Customer
10/25/2019
Application: WB
Species: Mouse

Verified Customer
03/27/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PPARG
Entrez
Uniprot