PPAR gamma/NR1C3 Antibody - BSA Free

Peroxisome proliferator-activated receptor gamma (PPARG), also known as nuclear receptor subfamily 1 group C member 3, NR1C3, GLM1, CIMT1, PPARG1, PPARG2, and PPARgamma, regulates adipocyte differentiation and binds peroxisome proliferators that include hypolipidemic drugs and fatty acids. PPARG regulates the peroxisomal beta-oxidation pathway of these fatty acids. PPARG activates transcription for acyl-CoA oxidase and regulates glucose homeostasis. PPARG is a member of the PPAR family, which is a subfamily of nuclear receptors. PPARG is found in the nucleus and the cytoplasm and is most prevalent in adipose tissue but also found in skeletal muscle, spleen, heart, liver, placenta, lung, and ovary. PPARG is found in three known isoforms (57.6, 54.8, and 21.6 kDa), which are produced by alternative splicing. The PPARG protein is subject to phosphorylation. PPARG has been linked to many diseases such as obesity, diabetes, atherosclerosis, and cancer. Recent research has indicated that mutations in PPARG play a role in non-alcoholic fatty liver disease (PMID: 22160635). Other research has found that using PPARG ligands as part of a chemotherapy treatment could help improve treatment for advanced pancreatic cancer (PMID: 22020928). Similar cancer research discovered that PPARG can be used to induce colorectal carcinoma cell death, making PPARG, in conjunction with survivin and caspase-3, a potential cancer cell regulator (PMID: 21765467).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: IF/IHC, In Vivo, WB.

Showing Publications 1 - 10 of 11. Show All 11 Publications. Collapse Publications.

Publications using NBP2-22106	Applications	Species
Hyo Lim Lee, Jong Min Kim, Min Ji Go, Seung Gyum Joo, Tae Yoon Kim, Han Su Lee, Ju Hui Kim, Jin-Sung Son, Ho Jin Heo Fermented Protaetia brevitarsis Larvae Ameliorates Chronic Ethanol-Induced Hepatotoxicity in Mice via AMPK and TLR-4/TGF-beta 1 Pathways Journal of Microbiology and Biotechnology 2024-03-28 [PMID: 38111317]
Wu J, Wang X, Zhang M et al. RXR Agonists Enhance Lenalidomide Anti-Myeloma Activity and T Cell Functions while Retaining Glucose-Lowering Effect Cells 2023-08-03 [PMID: 37566072] (In Vivo)	In Vivo
Moon S, Dilthey B, Guan S et al. Genetic deletion of skeletal muscle iPLA2? results in mitochondrial dysfunction, muscle atrophy and alterations in whole-body energy metabolism iScience 2023-06-01 [PMID: 37275531]
Wu J, Chu E, Paul B, Kang Y Mechanistic Studies and a Retrospective Cohort Study: The Interaction between PPAR Agonists and Immunomodulatory Agents in Multiple Myeloma Cancers 2022-10-27 [PMID: 36358696] (WB, Human)	WB	Human
Sha Y, Wu J, Paul B et al. PPAR agonists attenuate lenalidomide's anti-myeloma activity in vitro and in vivo Cancer letters 2022-07-21 [PMID: 35872263]
Tavallaee G, Lively S, Rockel JS et al. Contribution of microRNA-27b-3p to synovial fibrotic responses in knee osteoarthritis Arthritis & rheumatology (Hoboken, N.J.) 2022-07-06 [PMID: 35791923] (IF/IHC, Mouse)	IF/IHC	Mouse
Fidoamore A, Cristiano L, Laezza C et al. Energy metabolism in glioblastoma stem cells: PPARa a metabolic adaptor to intratumoral microenvironment Oncotarget 2017-12-12 [PMID: 29312541] (WB, Human)	WB	Human
Miller WR. PPARgamma agonism attenuates cocaine seeking behavior via association with ERk MAPk. Thesis. (WB, Rat)	WB	Rat
Li L, Gan H, Jin H, et al. Astragaloside IV promotes microglia/macrophages M2 polarization and enhances neurogenesis and angiogenesis through PPAR gamma pathway after cerebral ischemia/reperfusion injury in rats International immunopharmacology 2021-01-08 [PMID: 33429332]
Miller, CN;Samuels, JS;Azhar, Y;Parmar, A;Shashidharamurthy, R;Rayalam, S; Guggulsterone Activates Adipocyte Beiging through Direct Effects on 3T3-L1 Adipocytes and Indirect Effects Mediated through RAW264.7 Macrophages Medicines (Basel) 2019-01-31 [PMID: 30709026] (WB, Mouse)	WB	Mouse
Miller WR, Fox RG, Stutz SJ et al. PPARgamma agonism attenuates cocaine cue reactivity Addict Biol. 2016-11-11 [PMID: 27862692] Details: Citation using the HRP form of this antibody.
Show All 11 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Mouse	10/25/2019	View Summary Application Western Blot Sample Tested Adipose tissue Species Mouse Lot a-4 Comments Comments Mouse small intestine was homogenised and protein content was quantified by a BCA assay. Twenty micrograms of protein were resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membranes. Membranes were probed with primary antibody PPAR gamma diluted 1:1000 in 5% BSA 2, before incubation with Anti-rabbit secondary horseradish peroxidase-conjugated antibody 1:5000. Blots were visualised with Immobilon Western Chemiluminescence HRP Substrate and imaged with Syngene chemiluminescence imaging system.
Enlarge	3 reviewed by: Verified Customer	WB	Mouse	03/27/2018	View Summary Application Western Blot Sample Tested 3T3-L1 mouse embryonic fibroblast adipose-like cell line Species Mouse Lot A-3 Comments Comments Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-422106SS] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated on a 4-12% gel by SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk.

Array NBP2-22106

• PPAR gamma/NR1C3 Antibodies
• PPAR gamma/NR1C3 Kit
• PPAR gamma/NR1C3 Lysates
• PPAR gamma/NR1C3 Proteins