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PYK2/FAK2 [p Tyr402] Antibody (592918) [Unconjugated]

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Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 30 minutes and 10 µg/mL Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-PYK2/FAK2 (Y402) Monoclonal Antibody (Catalog # MAB6210) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Phospho-PYK2/FAK2 (Y402) at approximately 105-115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 30 minutes and 10 µg/mL Mouse ...read more
Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line untreated (-) or treated (+) with 0.2 mg/mL Pervanadate (PV) for 30 minutes, loaded at 0.2 mg/mL. A specific band was detected for ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clone
592918
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
Carrier Free
MAB6210-SP
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PYK2/FAK2 [p Tyr402] Antibody (592918) [Unconjugated] Summary

Immunogen
Phosphopeptide containing the human PYK2/FAK2 Y402 site
Accession # Q14289
Modification
p Tyr402
Specificity
Detects human PYK2/FAK2 when phosphorylated at Y402.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PTK2B
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 5
using
MAB6210 in the following applications:
Publications
Read Publication using
MAB6210 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PYK2/FAK2 [p Tyr402] Antibody (592918) [Unconjugated]

  • CADTK
  • CADTKCAK-beta
  • CAK beta
  • CAKB
  • Calcium-dependent tyrosine kinase
  • Cell adhesion kinase beta
  • EC 2.7.10
  • FADK2
  • FAK2
  • FAK2EC 2.7.10.2
  • Focal adhesion kinase 2
  • FRNK
  • PKB
  • ppyk2
  • Proline-rich tyrosine kinase 2
  • protein kinase B
  • protein tyrosine kinase 2 beta
  • protein-tyrosine kinase 2-beta
  • PTK
  • PTK2B protein tyrosine kinase 2 beta
  • PTK2B
  • PYK2
  • PYK2Related adhesion focal tyrosine kinase
  • RAFTK
  • RAFTKFADK 2

Background

PYK2 (Proline-rich kinase 2; also Ptk2b, Fak2, RAFTK and CAK beta ) is a 112‑116 kDa member of the Fak subfamily, tyrosine protein kinase family. It is expressed in multiple cell types, including endothelial cells, vascular smooth muscle cells, megakaryocytes and neurons. PYK2 is activated by elevated intracellular Ca++ and is associated with MAPK pathway activation. Human PYK2 is 1009 amino acids (aa) in length. PYK2 phosphorylation at Tyr402 is associated with enzymatic activation, intercellular localization, cell growth, cell motility, and regulating molecular associations. Over aa 390‑410, human PYK2 is 100% aa identical to mouse PYK2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PYK2/FAK2 Antibody (MAB6210)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Western Blot
(1)
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Review for PYK2/FAK2 Antibody (MAB6210) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB6210:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot PYK2/FAK2 MAB6210
Enlarge 		5
reviewed by:
Verified Customer
 WB Human 09/21/2018
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Summary

ApplicationWestern Blot
Sample TestedBreast cancer cells
SpeciesHuman
LotCDRO0114121

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Western Blot PYK2/FAK2 MAB6210
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 Verified Customer
09/21/2018
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol PTK2B
Uniprot