TLR2 Antibody (TL2.1) - Azide Free

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Canine.

We have publications tested in 8 applications: B/N, FLOW, Flow Cytometry Control, Flow-CS, ICC/IF, IHC-P, In vitro, WB.

Showing Publications 1 - 10 of 40. Show All 40 Publications. Collapse Publications.

Publications using NB100-56726	Applications	Species
Ji X, Zhang X et al The Heparin-Binding Hemagglutinin of Nocardia cyriacigeorgica GUH-2 Stimulates Inflammatory Cytokine Secretion Through Activation of Nuclear Factor kappa B and Mitogen-Activated Protein Kinase Pathways via TLR4 Front Cell Infect Microbiol 2020-03-03 [PMID: 32117792] (WB, WB, Human) Details: Citation using the Azide Free version of this antibody.	WB, WB	Human
The E, Yao Q, Zhang P et al. Mechanistic Roles of Matrilin-2 and Klotho in Modulating the Inflammatory Activity of Human Aortic Valve Cells Cells. [PMID: 32046115] (WB, Human)	WB	Human
Ji X, Zhang X, Li H et al. Nfa34810 facilitates Nocardia farcinica invasion of host cells and stimulates TNF-alpha secretion through activation of the NF-kappa B and MAPK pathways via TLR4 Infect Immun. [PMID: 31964749]
Li F, Song R, Ao L et al. ADAMTS5 Deficiency in Calcified Aortic Valves Is Associated With Elevated Pro-Osteogenic Activity in Valvular Interstitial Cells Arterioscler thromb Vasc Biol 2017-05-25 [PMID: 28546218] (WB, B/N, Human) Details: This reference used the Azide Free version of NB100-56722.	WB, B/N	Human
Kabisch R, Mejias-Luque R, Gerhard M, Prinz C. Involvement of Toll-Like Receptors on Helicobacter pylori-Induced Immunity. PLoS ONE. [PMID: 25153703] (B/N, Human) Details: Citation using the Azide Free form of this antibody.	B/N	Human
Rojas-Bernabe A, Garcia-Hernandez O, Maldonado-Bernal C et al. Leishmania mexicana lipophosphoglycan activates ERK and p38 MAP kinase and induces production of proinflammatory cytokines in human macrophages through TLR2 and TLR4. Parasitology [PMID: 24512642] (B/N, In vitro, Human) Details: Used the Azide Free form of this antibody.	B/N, In vitro	Human
Klink M, Brzezinska M, Szulc I et al. Cholesterol oxidase is indispensable in the pathogenesis of Mycobacterium tuberculosis PLoS One. PLoS One. 2013-09-09 [PMID: 24039915] (B/N, Human) Details: This citation used the PE version of this antibody.	B/N	Human
Tsai CC, Lin CR, Tsai HY et al. The immunologically active oligosaccharides isolated from wheatgrass modulate monocytes via Toll-like receptor-2 signaling J Biol Chem 2013-05-01 [PMID: 23629653]
Abdi J, Mutis T, Garssen J, Redegeld F. Characterization of the Toll-like receptor expression profile in human multiple myeloma cells. PLoS One. 2013-04-08 [PMID: 23593278] Details: Antibodies cited [multiple myeloma (MM) cell lines, human intestine tissue lysate, PBMC, mouse splenocytes] for WB (Figs 2, 3, 4A, 4B, 5, Table 1) and Flow (Intracellular): Figs 4C, 6-8, Table 1: 1. TLR1 (IMG-5012) 2. TLR2 (IMG-416A)3. TLR3 (IMG-315A)4 TL
Brzezinska M, Szulc I, Brzostek A et al. The role of 3-ketosteroid 1(2)-dehydrogenase in the pathogenicity of Mycobacterium tuberculosis. BMC Microbiol. [PMID: 23425360] (B/N, Human) Details: Citation using the Azide Free form of this antibody.	B/N	Human
Harman AN, Bye CR, Nasr N et al. Identification of lineage relationships and novel markers of blood and skin human dendritic cells. J Immunol. 2013-01-01 [PMID: 23183897] Details: Antibodies cited in Fig 4B for flow cytometric analysis of TLR expression in human monocyte-derived dendritic cells (MDDC), CD14+ monocytes, myeloid DC, and plasmacytoid DC:1. TLR2-FITC, clone T2.1 (IMG-416C): Flow (cell surface)2. TLR4-PE, clone HTA124 (
Shimizu T, Kida Y, Kuwano K. Cytoadherence-dependent induction of inflammatory responses by Mycoplasma pneumoniae. Immunology. 2011-05-01 [PMID: 21320122] (WB) Details: TLR2/CD282 (IMG-416A). WB: THP-1 cells treated with living or heat killed M. pneumoniae, Fig 3.	WB
Dasgupta G, Chentoufi AA, You S et al. Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses. Invest Ophthalmol Vis Sci. 2011-05-17 [PMID: 21273544] Details: Products cited: Flow (intracellular), Fig 1A ( CD4+CD25+ regulatory T cells purified from rabbit conjunctiva) & Fig 2A (CD11b+ cells isolated from rabbit conjunctiva): TLR2/CD282 PE (IMG-416D), TLR3/CD283 (IMG-315D), TLR4/CD284 (IMG-417D), TLR8/CD288 (IMG
Melchjorsen J, Rintahaka J, SOby S et al. Early innate recognition of herpes simplex virus in human primary macrophages is mediated via the MDA5/MAVS-dependent and MDA5/MAVS/RNA polymerase III-independent pathways. J Virol. [PMID: 20739519] Details: Citation using the Azide Free form of this antibody.
Oberg HH, Ly TT, Ussat S et al. Differential but direct abolishment of human regulatory T cell suppressive capacity by various TLR2 ligands. J Immunol. 2010-05-01 [PMID: 20363971] Details: Flow(intracellular) cytometry: TLR2/CD282 PE (IMG-416D) and TLR6/CD286 (IMG-304A). T cells and Tregs were separated from freshly isolated human PBMCs, Fig S3.
Kasari Villu, Kurg Kristi, Margus Tonu et al. The Escherichia coli mqsR and ygiT genes encode a new toxin-antitoxin pair. J Bacteriol. [PMID: 20233923] Details: Citation using the Azide Free form of this antibody.
Ding J, Rapista A, Teleshova N et al. Neisseria gonorrhoeae enhances HIV-1 infection of primary resting CD4+ T cells through TLR2 activation. J Immunol. 2010-03-15 [PMID: 20147631] (Flow-CS, Human) Details: TLR2/CD282 (IMG-416C). Flow (Cell surface), Fig 6D (human CD4+T cells).	Flow-CS	Human
Prabha C, Rajashree P, Sulochana DD. TLR2 and TLR4 expression on the immune cells of tuberculous pleural fluid. Immunol Lett. 2008-04-15 [PMID: 18295348] Details: TLR2- FITC (IMG-416C): Flow (cell surface): Figs. 1A, B (human CD4+T cells, CD8+T cells, B cells, CD16+56+ cells and monocytes); 2(CD4+T cells); 4A, B (human Treg cells). Flow (intracellular): Fig. 3A, B (CD4+T cells) 2. TLR4- FITC (IMG-417C).Flow (cell surface): Figs. 1B, C (human CD4+T cells, CD8+T cells, B cells, CD16+56+ cells and monocytes); 2(CD4+T cells); 4A, B (human Treg cells). Flow (intracellular): Fig. 3A, B (CD4+T cells).
Tsai CC, Lin CR, Tsai HY et al. The immunologically active oligosaccharides isolated from wheatgrass modulate monocytes via Toll-like receptor-2 signaling J Biol Chem Details: This publication used the FITC conjugated form of this antibody (Cat# NB100-56058) and PE conjugated form of this antibody (Cat# NBP2-24909) and the Biotin conjugated form of this antibody (Cat# NB100-56056).
Rydberg C, Mansson A, Uddman R et al. Toll-like receptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas. Immunology. 2009-09-01 [PMID: 19740321] Details: TLR2/CD282 PE (IMG-416D), TLR3/CD283 (IMG-315A), TLR3/CD283 (IMG-315D), TLR5 PE (IMG-663D), TLR5 (IMG-663). 1. IHC(paraffin): Human head and neck squamous (HNSCC) cell carcinomas showing keratinized cell carcinoma from the larynx stained with TLR2 (IMG-416A) and TLR5 antibodies (IMG-663A), Fig 1A, 1B, & 1C. 2. Flow (intracellular): TLR2 PE (IMG-416D), TLR3 PE (IMG-315D), and TLR5 PE (IMG-663D) antibodies were used in human bronchial epithelial (NL-20) and human pharyngeal squamous (Detroit-562) carcinoma cell line, Fig 2C.
Yang X, Fullerton DA, Su X et al Pro-osteogenic phenotype of human aortic valve interstitial cells is associated with higher levels of Toll-like receptors 2 and 4 and enhanced expression of bone morphogenetic protein 2. J Am Coll Cardiol. 2009-02-10 [PMID: 19195606] Details: Citation using the Azide Free version of this antibody.
Ji S, Shin JE, Kim YS et al. Toll-like receptor 2 and NALP2 mediate induction of human beta-defensins by fusobacterium nucleatum in gingival epithelial cells. Infect Immun 2009-03-01 [PMID: 19103770] (Flow-CS)	Flow-CS
Guzy C, Paclik D, Schirbel A et al. The probiotic Escherichia coli strain Nissle 1917 induces gammadelta T cell apoptosis via caspase- and FasL-dependent pathways. Int Immunol. 2008-07-01 [PMID: 18448456] Details: Antibodies cited [Flow (Cell Surface) (human PBMCs), Fig. 5]: 1. TLR2 FITC [IMG-416C] 2. TLR4 FITC [IMG-417C].
Burgener IA, Jungi TW. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008-07-15 [PMID: 18439687] Details: Canine (dog) PBMC subpopulations: 1. 10083K [IC-Flow (Intracellular Staining Flow Assay) Kit.
Pegu A, Qin S, Fallert Junecko BA et al. Human lymphatic endothelial cells express multiple functional TLRs. J Immunol. 2008-03-01 [PMID: 18292566] Details: Antibodies cited [Flow (Cell surface) and Flow (Intracellular), human lymphatic endothelial cells, Fig. 1D]: 1. IMG-663C (TLR5-FITC) 2. IMG-304C (TLR6-FITC).
Wong CK, Cheung PF, Ip WK et al. Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils. Am J Respir Cell Mol Biol. 2007-07-01 [PMID: 17332440] Details: Human blood eosinophils and neutrophils from buffy coat: For WB, Fig. 1A: TLR1 (IMG-5012), TLR5 (IMG-664), TLR6 (IMG-304A), TLR7 (IMG-540), TLR8 (IMG-321A), TLR9 (IMG-305A). For Flow (Intracellular) and Flow (Surface), Fig. 1B: TLR1 (IMG-5021), TLR2 (IMG-416C), TLR3 (IMG-315C), TLR4 (IMG-417C), TLR5 (IMG-663C), TLR6 (IMG-304C), TLR7 (IMG-665A), TLR8 (IMG-321C), TLR9 (IMG-305C).
Menzies BE, Kenoyer A. Signal transduction and nuclear responses in Staphylococcus aureus-induced expression of human beta-defensin 3 in skin keratinocytes. Infect Immun. 2006-12-01 [PMID: 16954397] (FLOW, Human) Details: TLR2 [IMG-416E] 1. FA (human keratinocytes), Fig. 2.	FLOW	Human
Xu S, Koldovsky U, Xu M et al High-avidity antitumor T-cell generation by toll receptor 8-primed, myeloid- derived dendritic cells is mediated by IL-12 production. Surgery. 2006-08-01 [PMID: 16904966] Details: Citation using the Azide Free version of this antibody.
Shimizu T, Kida Y, Kuwano K. A dipalmitoylated lipoprotein from Mycoplasma pneumoniae activates NF-kappa B through TLR1, TLR2, and TLR6. J Immunol. 2005-10-01 [PMID: 16177110] Details: TLR2 [IMG-416E] 1. FA (THP-1 cells), Fig. 2.
Srivastava MD, Srivastava BI. Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line. Leuk Res. 2005-07-01 [PMID: 15927677] (ICC/IF) Details: IF/ICC in SRIK-NKL cells: TLR2 (IMG416), TLR4 (IMG-417), TLR7 (IMG-581), and TLR9 (IMG-305).	ICC/IF
Cognasse F, Hamzeh H, Chavarin P et al. Evidence of Toll-like receptor molecules on human platelets. Immunol Cell Biol. 2005-04-01 [PMID: 15748217] Details: TLR2-PE (IMG-416D), TLR4-PE (IMG-417D), TLR6 (IMG-304A), TLR8-PE (IMG-321D), TLR9-PE (IMG-305D). Applications: Intracellular Flow Cytomety and Cell Surface Flow Cytometry: Figs 1 and 2. A comparison of staining results, intracellular versus cell surface flow cytometry is shown. Cell type: Human platelets.
Bazzocchi C, Mortarino M, Comazzi S et al. Expression and function of Toll-like receptor 2 in canine blood phagocytes. Vet Immunol Immunopathol. 2005-03-10 [PMID: 15661327] (Canine) Details: TLR2 [IMG-416A] 1. Flow (whole blood cells from dogs), Fig. 3.		Canine
Zhong F, Cao W, Chan E et al. Deviation from major codons in the Toll-like receptor genes is associated with low Toll-like receptor expression Immunology 2005-01-01 [PMID: 15606798] (Flow Cytometry Control) Details: This citation used the FITC version of this antibody.	Flow Cytometry Control
Gibson FC 3rd, Hong C, Chou HH et al. Innate immune recognition of invasive bacteria accelerates atherosclerosis in apolipoprotein E-deficient mice. Circulation. 2004-06-08 [PMID: 15123526] Details: TLR2 [IMG-416]: 1. IHC:P (aortic tissue), Fig. 2b 2. Flow (Cell Surface) (P gingivalis, mutant P gingivalis), Figs. 3a, 3b.
Mempel M, Voelcker V, Kollisch G et al. Toll-like receptor expression in human keratinocytes: nuclear factor kappaB controlled gene activation by Staphylococcus aureus is toll-like receptor 2 but not toll-like receptor 4 or platelet activating factor receptor dependent. J Invest Dermatol. 2003-12-01 [PMID: 14675188] (ICC/IF, Human) Details: TLR2 (IMG-416) 2. TLR4 (IMG-417) [IF/ICC, Fig.2A and 2D (human keratinocytes)].	ICC/IF	Human
Lotz Sonja, Aga Eresso, Wilde Inga et al. Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2. J Leukoc Biol. 2004-03-01 [PMID: 14673018]
Medvedev AE, Lentschat A, Wahl LM et al. Dysregulation of LPS-induced Toll-like receptor 4-MyD88 complex formation and IL-1 receptor-associated kinase 1 activation in endotoxin-tolerant cells. J Immunol. 2002-11-01 [PMID: 12391239] (WB, FLOW, Human) Details: TLR2 (IMG-416A): 1. WB (human monocytes), Fig. 3 2. Flow (Cell Surface) (human monocytes), Fig. 4.	WB, FLOW	Human
Flo TH, Halaas O, Torp S et al. Differential expression of Toll-like receptor 2 in human cells. J Leukoc Biol. 2001-03-01 [PMID: 11261796] (Flow-CS) Details: Antibodies cited: TLR2 (IMG-416): 1. Flow (Cell Surface): Figs 1 (whole blood), 5 (granulocytes), and 6 (monocytes) 2. IP: Fig 2 (monocytes) 3. IF/ICC: Fig 3 (monocyte-derived macrophages).	Flow-CS
Faure E, Thomas L, Xu H et al. Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NF-kappa B activation. J Immunol 2001-02-01 [PMID: 11160251] (WB, IHC-P) Details: This citation used the Biotin version of this antibody.	WB, IHC-P
Flo TH, Halaas O, Lien E et al. Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide. J Immunol. 2000-02-15 [PMID: 10657659] Details: TLR2 (IMG-416): 1. Generation of the IMG-416 TL2.1 antibody clone, recognizing a TLR2-associated epitope (Materials and Methods).
Show All 40 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Verified Customer	Flow	Human	12/29/2014	View Summary Application Flow Cytometry Sample Tested See PMID 23425360 Species Human
	4 reviewed by: Verified Customer	Flow	Human	12/29/2014	View Summary Application Flow Cytometry Sample Tested See PMID 24039915 Species Human

Array NB100-56726

• TLR2 Antibodies
• TLR2 ELISA Kits
• TLR2 Lysates
• TLR2 Proteins
• TLR2 ELISA
• TLR2 Proteins and Enzymes