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Human CCL2/MCP-1 Quantikine ELISA Kit

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

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Catalog# & Conjugate Size Price

Human CCL2/MCP-1 Quantikine ELISA Kit Summary

Background
The Quantikine Human MCP-1 Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure MCP-1 in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human MCP-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human MCP-1. Results obtained using natural human ...MCP-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MCP-1.
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Specificity
Natural and recombinant human MCP-1
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
CCL2

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DCP00.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CCL2/MCP-1 Quantikine ELISA Kit

  • C-C motif chemokine ligand 2
  • CCL2
  • GDCF-2
  • HC11
  • HSMCR30
  • MCAF
  • Mcp1
  • MCP-1
  • SCYA2
  • SMC-CF

Background

CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4+ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CCL2/JE/MCP-1 (DCP00)(250)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Fish, Porcine, Primate - Chlorocebus aethiops (African Green Monkey), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque).


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Human
(231)
Mouse
(2)
Rat
(3)
Fish
(1)
Porcine
(3)
Primate - Chlorocebus aethiops (African Green Monkey)
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(2)
Primate - Macaca mulatta (Rhesus Macaque)
(7)
All Species
Showing Publications 1 - 10 of 250. Show All 250 Publications.
Publications using DCP00 Applications Species
Brüser, L;Teichmann, E;Hinz, B; Effect of Flavonoids on MCP-1 Expression in Human Coronary Artery Endothelial Cells and Impact on MCP-1-Dependent Migration of Human Monocytes International journal of molecular sciences 2023-11-07 [PMID: 38003237] (Human) Human
Jalan-Sakrikar, N;Anwar, A;Yaqoob, U;Gan, C;Lagnado, AB;Wixom, AQ;Jurk, D;Huebert, RC; Telomere dysfunction promotes cholangiocyte senescence and biliary fibrosis in Primary Sclerosing Cholangitis JCI insight 2023-09-14 [PMID: 37707950] (Human) Human
F Bruschi, B Pinto, P Fallahi, SM Ferrari, A Antonelli Increased neutrophil derived chemokines (CXCL10 and CCL2) in human trichinellosis as possible serological markers of the polarization of the immune response against the parasite Cytokine, 2023-04-12;166(0):156205. 2023-04-12 [PMID: 37058963] (Human) Human
Abasubong, KP;Jiang, GZ;Guo, HX;Wang, X;Huang, YY;Dai, YJ;Li, XF;Yanzou, D;Gabriel, NN;Liu, WB; Oral bovine serum albumin administration alleviates inflammatory signals and improves antioxidant capacity and immune response under thioacetamide stress in blunt snout bream fed a high-calorie diet Fish & shellfish immunology 2023-08-12 [PMID: 37579810] (Fish) Fish
Klersy, A;Meyer, S;Leuschner, F;Kessler, T;Hecker, M;Wagner, AH; Ectodomain Shedding by ADAM17 Increases the Release of Soluble CD40 from Human Endothelial Cells under Pro-Inflammatory Conditions Cells 2023-07-25 [PMID: 37566005] (Human) Human
Peng, J;Lin, Z;Chen, W;Ruan, J;Deng, F;Yao, L;Rao, M;Xiong, X;Xu, S;Zhang, X;Liu, X;Sun, X; Vemurafenib induces a noncanonical senescence-associated secretory phenotype in melanoma cells which promotes vemurafenib resistance Heliyon 2023-01-01 [PMID: 37456058] (Human) Human
Chow, FC;Mundada, NS;Abohashem, S;La Joie, R;Iaccarino, L;Arechiga, VM;Swaminathan, S;Rabinovici, GD;Epel, ES;Tawakol, A;Hsue, PY; Psychological stress is associated with arterial inflammation in people living with treated HIV infection Brain, behavior, and immunity 2023-06-25 [PMID: 37369339] (Human) Human
Kim, MJ;Kim, JY;Shin, JH;Kang, Y;Lee, JS;Son, J;Jeong, SK;Kim, D;Kim, DH;Chun, E;Lee, KY; FFAR2 antagonizes TLR2- and TLR3-induced lung cancer progression via the inhibition of AMPK-TAK1 signaling axis for the activation of NF-?B Cell & bioscience 2023-06-07 [PMID: 37287005] (Human) Human
F Parween, SP Singh, HH Zhang, N Kathuria, FA Otaizo-Car, A Shamsaddin, PJ Gardina, S Ganesan, J Kabat, HA Lorenzi, TG Myers, JM Farber Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells bioRxiv : the preprint server for biology, 2023-02-13;0(0):. 2023-02-13 [PMID: 36789428] (Human) Human
Show All 250 Publications.

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Bioinformatics

Gene Symbol CCL2