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Human CCL2/MCP-1 Quantikine ELISA Kit

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Human CCL2/MCP-1 Quantikine ELISA Kit Summary

Background
The Quantikine Human MCP-1 Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure MCP-1 in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human MCP-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human MCP-1. Results obtained using natural human ...MCP-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MCP-1.
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Specificity
Natural and recombinant human MCP-1
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
CCL2

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DCP00.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CCL2/MCP-1 Quantikine ELISA Kit

  • C-C motif chemokine ligand 2
  • CCL2
  • GDCF-2
  • HC11
  • HSMCR30
  • MCAF
  • Mcp1
  • MCP-1
  • SCYA2
  • SMC-CF

Background

CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4+ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CCL2/JE/MCP-1 (DCP00)(255)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Fish, Porcine, Primate - Chlorocebus aethiops (African Green Monkey), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque).


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Human
(236)
Mouse
(2)
Rat
(3)
Fish
(1)
Porcine
(3)
Primate - Chlorocebus aethiops (African Green Monkey)
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(2)
Primate - Macaca mulatta (Rhesus Macaque)
(7)
All Species
Showing Publications 1 - 10 of 255. Show All 255 Publications.
Publications using DCP00 Applications Species
Longo, M;Paolini, E;Di Benedetto, P;Tomassini, E;Meroni, M;Dongiovanni, P; DGAT1 and DGAT2 Inhibitors for Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) Management: Benefits for Their Single or Combined Application International journal of molecular sciences 2024-08-21 [PMID: 39201759] (Human) Human
Brüser, L;Teichmann, E;Hinz, B; Effect of Flavonoids on MCP-1 Expression in Human Coronary Artery Endothelial Cells and Impact on MCP-1-Dependent Migration of Human Monocytes International journal of molecular sciences 2023-11-07 [PMID: 38003237] (Human) Human
Iacovacci, J;Serafini, MS;Avuzzi, B;Badenchini, F;Cicchetti, A;Devecchi, A;Dispinzieri, M;Doldi, V;Giandini, T;Gioscio, E;Mancinelli, E;Noris Chiorda, B;Orlandi, E;Palorini, F;Possenti, L;Reis Ferreira, M;Villa, S;Zaffaroni, N;De Cecco, L;Valdagni, R;Rancati, T; Intestinal microbiota composition is predictive of radiotherapy-induced acute gastrointestinal toxicity in prostate cancer patients EBioMedicine 2024-07-18 [PMID: 39029427] (Human) Human
Scovino, AM;Dahab, EC;Diniz-Lima, I;de Senna Silveira, E;Barroso, SPC;Cardoso, KM;Nico, D;Makhoul, GJ;da Silva-Junior, EB;Freire-de-Lima, CG;Freire-de-Lima, L;Fonseca, LMD;Valente, N;Nacife, V;Machado, A;Araújo, M;Vieira, GF;Pauvolid-Corrêa, A;Siqueira, M;Morrot, A; A Comparative Analysis of Innate Immune Responses and the Structural Characterization of Spike from SARS-CoV-2 Gamma Variants and Subvariants Microorganisms 2024-04-02 [PMID: 38674664] (Human) Human
Sorenson, CM;Gurel, Z;Song, YS;Peterson, KD;Blodi, BA;Sheibani, N; Thrombospondin-1, BIM and CFH polymorphisms and response to anti-VEGF treatment in neovascular age- related macular degeneration patients PloS one 2024-02-26 [PMID: 38408093] (Human) Human
Vilella, A;Bodria, M;Papotti, B;Zanotti, I;Zimetti, F;Remaggi, G;Elviri, L;Potì, F;Ferri, N;Lupo, MG;Panighel, G;Daini, E;Vandini, E;Zoli, M;Giuliani, D;Bernini, F; PCSK9 ablation attenuates A? pathology, neuroinflammation and cognitive dysfunctions in 5XFAD mice Brain, behavior, and immunity 2023-11-13 [PMID: 37967665] (Human) Human
Jalan-Sakrikar, N;Anwar, A;Yaqoob, U;Gan, C;Lagnado, AB;Wixom, AQ;Jurk, D;Huebert, RC; Telomere dysfunction promotes cholangiocyte senescence and biliary fibrosis in Primary Sclerosing Cholangitis JCI insight 2023-09-14 [PMID: 37707950] (Human) Human
Abasubong, KP;Jiang, GZ;Guo, HX;Wang, X;Huang, YY;Dai, YJ;Li, XF;Yanzou, D;Gabriel, NN;Liu, WB; Oral bovine serum albumin administration alleviates inflammatory signals and improves antioxidant capacity and immune response under thioacetamide stress in blunt snout bream fed a high-calorie diet Fish & shellfish immunology 2023-08-12 [PMID: 37579810] (Fish) Fish
Klersy, A;Meyer, S;Leuschner, F;Kessler, T;Hecker, M;Wagner, AH; Ectodomain Shedding by ADAM17 Increases the Release of Soluble CD40 from Human Endothelial Cells under Pro-Inflammatory Conditions Cells 2023-07-25 [PMID: 37566005] (Human) Human
Show All 255 Publications.

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Bioinformatics

Gene Symbol CCL2