Novus Biologicals products are now on bio-techne.com

MAT1/2A Antibody - BSA Free

Images

 
Western Blot: MAT1/2A Antibody [NB110-94162] - Detection of MAT2A in HepG2 whole cell lysates using NB110-94162.
Simple Western: MAT1/2A Antibody [NB110-94162] - Simple Western lane view shows a specific band for MAT1/2 A in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Rt, Bv, Pm, Ze, Mu(-)Species Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

MAT1/2A Antibody - BSA Free Summary

Immunogen
Synthetic peptide made to an internal portion of the human MAT2A protein (within residues 100-200). [Swiss-Prot# P31153]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAT2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Simple Western 1:10
  • Western Blot 2 ug/ml
Application Notes
This MAT1/2A antibody is useful for Western blot, where a band is seen at approx. 43 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
MAT2A Overexpression Lysate
Control Peptide
MAT1/2A Peptide (NB110-94162PEP)
Reviewed Applications
Read 1 Review rated 5
using
NB110-94162 in the following applications:

Publications
Read Publications using
NB110-94162 in the following applications:

  • WB
    1 publication

Reactivity Notes

Orangutan. Does not react with mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MAT1/2A Antibody - BSA Free

  • AdoMet synthase 2
  • AMS2
  • EC 2.5.1.6
  • MAT 2
  • MATA2SAMS2adoMet synthetase 2
  • MATII
  • MAT-II
  • Methionine adenosyltransferase 2
  • Methionine adenosyltransferase II
  • methionine adenosyltransferase II, alpha
  • S-adenosylmethionine synthase isoform type-2
  • S-adenosylmethionine synthetase isoform type-2

Background

MAT1A and MAT2A are catalytic subunits of methionine adenosyltransferase (MAT), which catalyzes the formation of S-adenosyltransferase for methionine catabolism. Inactivation of MAT1A has been linked to liver disease. In addition, in certain liver cancers expression of MAT1A is replaced by expression of MAT1B. Overexpression of MAT2A has also been observed in colon cancer, leading to the proposal that MAT2A facilitates cancer cell growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP3-13066
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP3-13785
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC-P, IP, PA, WB
NBP3-13389
Species: Hu
Applications: IHC, IHC-P, WB
NBP3-04894
Species: Hu
Applications: ELISA, IHC, IHC-P, KO, WB
NBP1-31528
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP2-48817
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
MAB8930
Species: Hu
Applications: ICC, Simple Western, WB
NBP1-82797
Species: Hu, Rt
Applications: IHC, IHC-P, WB
NBP1-89512
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-32914
Species: Hu, Mu
Applications: ICC/IF, IHC, WB
294-HG
Species: Hu
Applications: BA
NBP2-45402
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
AF5215
Species: Hu, Mu
Applications: ICC, WB
NB100-1923
Species: Ce
Applications: IHC, IHC-P, IP, WB
NBP1-80306
Species: Av, Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
AF3989
Species: Hu, Mu, Rt
Applications: WB
NBP2-50037
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
NBP2-45570
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
NB110-94162
Species: Hu, Rt, Bv, Pm, Ze, Mu(-)
Applications: WB, Simple Western

Publications for MAT1/2A Antibody (NB110-94162)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Review for MAT1/2A Antibody (NB110-94162) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB110-94162:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot MAT1/2A NB110-94162
Enlarge
5
reviewed by:
Steve Smith
WB Human 12/14/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate prepared from RT-4 cells
SpeciesHuman

Product General Protocols

View specific protocols for MAT1/2A Antibody (NB110-94162): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MAT1/2A Antibody (NB110-94162) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional MAT1/2A Products

Research Areas for MAT1/2A Antibody (NB110-94162)

Find related products by research area.

Blogs on MAT1/2A

There are no specific blogs for MAT1/2A, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Steve Smith
12/14/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol MAT2A