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Serum Response Factor SRF Antibody (PCRP-SRF-1F7)

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Immunocytochemistry/Immunofluorescence: Serum Response Factor SRF Antibody (PCRP-SRF-1F7) [NBP3-13785] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Serum Response Factor SRF Antibody (PCRP-SRF-1F7) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).
Immunocytochemistry/ Immunofluorescence: Serum Response Factor SRF Antibody (PCRP-SRF-1F7) [NBP3-13785] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Serum Response Factor SRF Antibody ...read more
Immunohistochemistry-Paraffin: Serum Response Factor SRF Antibody (PCRP-SRF-1F7) [NBP3-13785] - IHC analysis of formalin-fixed, paraffin-embedded human colon. Strong nuclear staining using Serum Response Factor SRF ...read more
Flow Cytometry: Serum Response Factor SRF Antibody (PCRP-SRF-1F7) [NBP3-13785] - Flow cytometric analysis of PFA-fixed HeLa cells. Serum Response Factor SRF antibody (PCRP-SRF-1F7) followed by goat anti-mouse IgG-CF488 ...read more
Protein Array: Serum Response Factor SRF Antibody (PCRP-SRF-1F7) [NBP3-13785] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Serum Response Factor SRF Antibody (PCRP-SRF-1F7).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP, MA
Clone
PCRP-SRF-1F7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-14188AF350
Alexa Fluor 405
NBP3-14188AF405
Alexa Fluor 488
NBP3-14188AF488
Alexa Fluor 532
NBP3-14188AF532
Alexa Fluor 594
NBP3-14188AF594
Alexa Fluor 647
NBP3-14188AF647
Alexa Fluor 700
NBP3-14188AF700
Alexa Fluor 750
NBP3-14188AF750
Allophycocyanin
NBP3-14188APC
Biotin
NBP3-14188B
CoraFluor 1
NBP3-14188CL1
DyLight 350
NBP3-14188UV
DyLight 405
NBP3-14188V
DyLight 488
NBP3-14188G
DyLight 550
NBP3-14188R
DyLight 594
NBP3-14188DL594
DyLight 650
NBP3-14188C
DyLight 680
NBP3-14188FR
DyLight 755
NBP3-14188IR
FITC
NBP3-14188F
HRP
NBP3-14188H
Janelia Fluor 525
NBP3-14188JF525
Janelia Fluor 549
NBP3-14188JF549
Janelia Fluor 646
NBP3-14188JF646
Janelia Fluor 669
NBP3-14188JF669
PE
NBP3-14188PE
PerCP
NBP3-14188PCP
mFluor Violet 450 SE
NBP3-14188MFV450
mFluor Violet 500 SE
NBP3-14188MFV500
mFluor Violet 610 SE
NBP3-14188MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-14188
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Serum Response Factor SRF Antibody (PCRP-SRF-1F7) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14188)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Non-hazardous.

Theoretical molecular weight: 40-67kDa
Immunogen
Recombinant full-length human Serum Response Factor SRF protein (Uniprot: P11831)
Localization
Nucleus, Nucleoplasm
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SRF
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Serum Response Factor SRF Antibody (PCRP-SRF-1F7)

  • MCM1
  • serum response factor (c-fos serum response element-binding transcriptionfactor)
  • Serum Response Factor SRF
  • serum response factor
  • SRF

Background

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is a cardiac-enriched transcription factor that is required for the appearance of beating sarcomeres in the heart. In addition, SRF may also direct the expression of microRNAs that inhibit the expression of cardiac regulatory factors. SRF binding sites are also constitutive promotor elements in many muscle specific promoters. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk 1 and SRF accessory protein 1 (SAP 1) have DNA binding properties identical to that of p62TCF

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Serum Response
Factor SRF Antibody ( ...
NBP3-13785
Serum Response Factor SRF Antibody (PCRP-SRF-1F7)
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, MA

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Bioinformatics

Gene Symbol SRF
Uniprot