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Recombinant Human HGF Protein

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Recombinant Human HGF (294-HG) induces IL-11 secretion in Saos-2 human osteosarcoma cells. The ED50 for this effect is equal to less than 4.00 ng/mL.
Recombinant Human HGF (294-HG) induces IL-11 secretion in Saos-2 human osteosarcoma cells. The ED50 for this effect is equal to less than 4.00 ng/mL.
Adult stem cell-derived liver organoids were cultured using Cultrex UltiMatrix RGF Basement Membrane Extract (BME001-05) and liver organoid initiation medium, which includes Recombinant Human HGF (Catalog # 294-HG), ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
294-HG-005/CF
Carrier Free
294-HG-025/CF
Carrier Free
294-HG-100/CF
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Recombinant Human HGF Protein Summary

Details of Functionality
Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883. The ED50 for this effect is equal to or less than 4.00 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human HGF protein
Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)
Accession #
N-terminal Sequence
alpha chain: No results obtained; Gln32 predicted
beta chain: Val495
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Gene
HGF
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
53.7 kDa ( alpha chain), 26 kDa ( beta chain).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60-70 kDa, non-reducing conditions
60 kDa, and 33-34 kDa, reducing conditions
Publications
Read Publications using
294-HG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human HGF Protein

  • deafness, autosomal recessive 39
  • DFNB39
  • EC 3.4.21
  • EC 3.4.21.7
  • fibroblast-derived tumor cytotoxic factor
  • F-TCF
  • hepatocyte growth factor (hepapoietin A; scatter factor)
  • Hepatopoeitin-A
  • Hepatopoietin A
  • HGF
  • HGFB
  • HPTA
  • HPTAhepatocyte growth factor
  • lung fibroblast-derived mitogen
  • Scatter factor
  • SF
  • SFhepatopoeitin-A

Background

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE‑like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1‑5). Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive
disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta  chain. Alternate splicing generates human HGF isoforms that lack the proteinase‑like domain and different numbers of the Kringle domains. Human HGF shares 91%‑94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up‑regulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin‑1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH‑stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

  1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
  2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
  3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
  4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
  5. Nakamura, T. et al. (1989) Nature 342:440.
  6. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
  7. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
  8. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  9. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
  10. Montesano, R. et al. (1991) Cell 67:901.
  11. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
  12. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
  13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
  14. Mineo, R. et al. (1994) Endocrinology 145:4355.
  15. Urbanek, K. et al. (2005) Circ. Res. 97:663.

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Recombinant Human
HGF Protein
294-HG
Recombinant Human HGF Protein
Species: Hu
Applications: Bioactivity

Publications for HGF (294-HG)(113)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Equine, Fish - Prionace glauca (Blue Shark), Hamster, N/A, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Squirrel.

We have publications tested in 7 applications: Activation, Bioassay, Cell Culture, Control, ELISA (Standard), In Vivo, Proliferation.


Filter By Application
Activation
(1)
Bioassay
(99)
Cell Culture
(7)
Control
(1)
ELISA (Standard)
(1)
In Vivo
(2)
Proliferation
(1)
All Applications
Filter By Species
Human
(91)
Mouse
(6)
Rat
(6)
Equine
(1)
Fish - Prionace glauca (Blue Shark)
(1)
Hamster
(1)
N/A
(1)
Porcine
(2)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Squirrel
(1)
All Species
Showing Publications 1 - 10 of 113. Show All 113 Publications.
Publications using 294-HG Applications Species
Gao, H;Huang, X;Cai, Z;Cai, B;Wang, K;Li, J;Kuang, J;Wang, B;Zhai, Z;Ming, J;Cao, S;Qin, Y;Pei, D; Generation of musculoskeletal cells from human urine epithelium-derived presomitic mesoderm cells Cell & bioscience 2024-07-15 [PMID: 39010176] (Bioassay, Human) Bioassay Human
Mozin, E;Massouridès, E;Mournetas, V;Lièvre, C;Bourdon, A;Jackson, DL;Packer, JS;Trapnell, C;Guiner, CL;Adjali, O;Pinset, C;Mack, DL;Dupont, JB; Dystrophin deficiency impairs cell junction formation during embryonic myogenesis bioRxiv : the preprint server for biology 2023-12-07 [PMID: 38106055] (Bioassay, Human) Bioassay Human
Gesualdi, L;Berardini, M;Scicchitano, B;Castaldo, C;Bizzarri, M;Filippini, A;Riccioli, A;Schiraldi, C;Ferranti, F;Liguoro, D;Mancini, R;Ricci, G;Catizone, A; ERK Signaling Pathway Is Constitutively Active in NT2D1 Non-Seminoma Cells and Its Inhibition Impairs Basal and HGF-Activated Cell Proliferation Biomedicines 2023-01-01 [PMID: 37509533] (Bioassay, Human) Bioassay Human
M Carbonaro, K Wang, H Huang, D Frleta, A Patel, A Pennington, M Desclaux, S Moller-Tan, J Grindley, J Altarejos, J Zhong, G Polites, W Poueymirou, S Jaspers, C Kyratsous, B Zambrowicz, A Murphy, JC Lin, LE Macdonald, C Daly, M Sleeman, G Thurston, Z Li IL-6-GP130 signaling protects human hepatocytes against lipid droplet accumulation in humanized liver models Science Advances, 2023-04-14;9(15):eadf4490. 2023-04-14 [PMID: 37058568] (Bioassay, Human) Bioassay Human
G Rizzo, SE Pineda Cha, E Vandenkoor, CL Cárdenas R, V Cento, V Garlatti, M Wozny, G Sammarco, A Di Claudio, L Meanti, S Elangovan, A Romano, G Roda, L Loy, A Dal Buono, R Gabbiadini, S Lovisa, R Rusconi, A Repici, A Armuzzi, S Vetrano Pomegranate Extract Affects Gut Biofilm Forming Bacteria and Promotes Intestinal Mucosal Healing Regulating the Crosstalk between Epithelial Cells and Intestinal Fibroblasts Nutrients, 2023-04-05;15(7):. 2023-04-05 [PMID: 37049615] (Bioassay, Human) Bioassay Human
S Wenhua, T Tsunematsu, M Umeda, H Tawara, N Fujiwara, Y Mouri, R Arakaki, N Ishimaru, Y Kudo Cancer cell-derived novel periostin isoform promotes invasion in head and neck squamous cell carcinoma Cancer Medicine, 2023-01-23;0(0):. 2023-01-23 [PMID: 36691359] (Bioassay, Human) Bioassay Human
JE Kaserman, RB Werder, F Wang, T Matte, MI Higgins, M Dodge, J Lindstrom-, P Bawa, A Hinds, E Bullitt, IS Caballero, X Shi, RE Gerszten, N Brunetti-P, M Liesa, C Villacorta, AN Hollenberg, DN Kotton, AA Wilson Human iPSC-hepatocyte modeling of alpha-1 antitrypsin heterozygosity reveals metabolic dysregulation and cellular heterogeneity Cell Reports, 2022-12-06;41(10):111775. 2022-12-06 [PMID: 36476855] (Bioassay, Human) Bioassay Human
R Yoshida, M Saigi, T Tani, BF Springer, H Shibata, S Kitajima, NR Mahadevan, M Campisi, W Kim, Y Kobayashi, TC Thai, K Haratani, Y Yamamoto, SK Sundararam, EH Knelson, A Vajdi, I Canadas, R Uppaluri, CP Paweletz, JJ Miret, PH Lizotte, PC Gokhale, PA Janne, DA Barbie MET-induced CD73 restrains STING-mediated immunogenicity of EGFR-mutant lung cancer Cancer Research, 2022-11-02;0(0):. 2022-11-02 [PMID: 36066413] (Bioassay, Human) Bioassay Human
Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, Varjosalo M Physical and functional interactome atlas of human receptor tyrosine kinases. EMBO Rep, 2022-04-05;23(6):e54041. 2022-04-05 [PMID: 35384245] (Bioassay, Human) Bioassay Human
J Krumm, K Sekine, P Samaras, A Brazovskaj, M Breunig, R Yasui, A Kleger, H Taniguchi, M Wilhelm, B Treutlein, JG Camp, B Kuster High temporal resolution proteome and phosphoproteome profiling of stem cell-derived hepatocyte development Cell Reports, 2022-03-29;38(13):110604. 2022-03-29 [PMID: 35354033] (Bioassay, Human) Bioassay Human
Show All 113 Publications.

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Bioinformatics

Gene Symbol HGF
Uniprot