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UBE2J1 Antibody (OTI3D1)

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Western Blot: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western Blot: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; ...read more
Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Carcinoma of Human prostate tissue.
Flow Cytometry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Jurkat cells, using UBE2J1 antibody,(Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2J1.
Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Adenocarcinoma of Human colon tissue.
Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Adenocarcinoma of Human ovary tissue.
Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Human tonsil tissue.
Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Human lung tissue.
Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Human lymphoma tissue.
Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Adenocarcinoma of Human endometrium tissue.
Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Carcinoma of Human pancreas tissue.
Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Adenocarcinoma of Human breast tissue.
Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Carcinoma of Human liver tissue.
Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Human breast tissue.
Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Human pancreas tissue.
Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Carcinoma of Human lung tissue.
Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Human Kidney tissue.
Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Human prostate tissue.
Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Human liver tissue.
Flow Cytometry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Hela cells, using UBE2J1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI3D1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74747AF350
Alexa Fluor 405
NBP2-74747AF405
Alexa Fluor 488
NBP2-74747AF488
Alexa Fluor 532
NBP2-74747AF532
Alexa Fluor 594
NBP2-74747AF594
Alexa Fluor 647
NBP2-74747AF647
Alexa Fluor 700
NBP2-74747AF700
Alexa Fluor 750
NBP2-74747AF750
Allophycocyanin
NBP2-74747APC
Allophycocyanin/Cy7
NBP2-74747APCCY7
Biotin
NBP2-74747B
CoraFluor 1
NBP2-74747CL1
DyLight 350
NBP2-74747UV
DyLight 405
NBP2-74747V
DyLight 488
NBP2-74747G
DyLight 550
NBP2-74747R
DyLight 594
NBP2-74747DL594
DyLight 650
NBP2-74747C
DyLight 680
NBP2-74747FR
DyLight 755
NBP2-74747IR
FITC
NBP2-74747F
HRP
NBP2-74747H
Janelia Fluor 525
NBP2-74747JF525
Janelia Fluor 549
NBP2-74747JF549
Janelia Fluor 585
NBP2-74747JF585
Janelia Fluor 635
NBP2-74747JF635
Janelia Fluor 646
NBP2-74747JF646
Janelia Fluor 669
NBP2-74747JF669
PE
NBP2-74747PE
PE/Atto594
NBP2-74747PEATT594
PE/Cy5.5
NBP2-74747PECY55
PE/Cy7
NBP2-74747PECY7
PerCP
NBP2-74747PCP
mFluor Violet 450 SE
NBP2-74747MFV450
mFluor Violet 500 SE
NBP2-74747MFV500
mFluor Violet 610 SE
NBP2-74747MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-74747
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UBE2J1 Antibody (OTI3D1) Summary

Immunogen
Full length human recombinant protein of human UBE2J1(NP_057105) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
UBE2J1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UBE2J1 Antibody (OTI3D1)

  • CGI-76
  • EC 6.3.2.19
  • HSPC153
  • HSPC205
  • HSU93243
  • HsUBC6e
  • MGC12555
  • NCUBE1
  • NCUBE-1
  • NCUBE1HSUBC6e
  • Non-canonical ubiquitin-conjugating enzyme 1
  • non-canonical ubquitin conjugating enzyme 1
  • UBC6e
  • Ubc6p
  • UBE2J1
  • ubiquitin-conjugating enzyme E2 J1
  • ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast)
  • Yeast ubiquitin-conjugating enzyme UBC6 homolog E

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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UBE2J1 Antibody
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Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, Flow, IHC

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Bioinformatics

Gene Symbol UBE2J1