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MAT2B Antibody

Images

 
Western Blot: MAT2B Antibody [NBP1-82797] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: MAT2B Antibody [NBP1-82797] - Staining of human skin shows no positivity in keratinocytes as expected.
Immunohistochemistry-Paraffin: MAT2B Antibody [NBP1-82797] - Staining of human kidney shows moderate cytoplasmic and nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: MAT2B Antibody [NBP1-82797] - Staining of human duodenum shows moderate positivity in luminal membrane in glandular cells.
Immunohistochemistry-Paraffin: MAT2B Antibody [NBP1-82797] - Staining of human liver shows moderate positivity in bile canaliculi in hepatocytes.
Immunohistochemistry-Paraffin: MAT2B Antibody [NBP1-82797] - Staining of human cerebral cortex shows moderate positivity in neuronal processes in astrocytes.

Product Details

Summary
Reactivity Hu, Rt, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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MAT2B Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MFDKVQFSNKSANMDHWQQRFPTHVKDVATVCRQLAEKRMLDPSIKGTFHWSGNEQMTKYEMACAIADAFN
Predicted Species
Mouse (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAT2B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
MAT2B Protein (NBP1-82797PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-82797 in the following applications:

Publications
Read Publications using
NBP1-82797 in the following applications:

  • WB
    3 publications

Reactivity Notes

Rat reactivity reported in the scientific literature (PMID: 20043323).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MAT2B Antibody

  • beta regulatory subunit of methionine adenosyltransferase
  • DTDP-4-keto-6-deoxy-D-glucose 4-reductase
  • MAT II beta
  • MAT-II
  • MATIIbeta
  • methionine adenosyltransferase 2 subunit beta
  • Methionine adenosyltransferase II beta
  • methionine adenosyltransferase II, beta
  • MGC12237
  • Nbla02999
  • putative protein product of Nbla02999
  • SDR23E1
  • short chain dehydrogenase/reductase family 23E, member 1
  • TGR

Background

MAT2B is encoded by this gene belongs to the methionine adenosyltransferase (MAT) family. MAT catalyzes the biosynthesis of S-adenosylmethionine from methionine and ATP. This protein is the regulatory beta subunit of MAT. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-82797
Species: Hu, Rt, Mu
Applications: WB, IHC

Publications for MAT2B Antibody (NBP1-82797)(3)

Review for MAT2B Antibody (NBP1-82797) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-82797:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot MAT2B NBP1-82797
Enlarge
4
reviewed by:
Steve Smith
WB Human 12/14/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate prepared from RT-4 cells
SpeciesHuman

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for MAT2B Antibody (NBP1-82797) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional MAT2B Products

Research Areas for MAT2B Antibody (NBP1-82797)

Find related products by research area.

Blogs on MAT2B.

MAT2a, MAT2b, HIF-1 alpha: Roles in Liver Cancer and DNA methylation
Methionine Adenosyltransferase II alpha, also known as MAT2a, is a catalytic subunit of methionine adenosyltransferase (MAT) and essential enzyme for the catalysis of the principle biological methyl donor, S-adenosylmethionine (SAM) from methionine an...  Read full blog post.

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Recent Reviews

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5
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4
1
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1
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Steve Smith
12/14/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol MAT2B
Entrez
Uniprot