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c-jun [p Ser63] Antibody (1018Y) [Unconjugated]

 (3 Publications)
 (1 Review)
 
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Western blot shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm2ultraviolet light (UV) with a 30 minute recovery. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-c-Jun (S63) Monoclonal Antibody (Catalog # MAB8930) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-c-Jun (S63) at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm2ultraviolet light (UV) with a 30 minute recovery. PVDF membrane was probed with 0.5 µg/mL of Rabbit ...read more
c-Jun phosphorylated at S63 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with Anisomycin using Rabbit Anti-Human Phospho-c-Jun (S63) Monoclonal Antibody (Catalog # MAB8930) ...read more
Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm2 ultraviolet light (UV) with a 30 minute recovery, loaded at 0.2 mg/mL. A specific band was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clone
1018Y
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB8930-SP
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c-jun [p Ser63] Antibody (1018Y) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Phosphopeptide containing the human c-Jun S63 site
Accession # P05412
Modification
p Ser63
Specificity
Detects human c-Jun when phosphorylated at S63 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
JUN
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 1-25 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB8930 in the following applications:
Publications
Read Publications using
MAB8930 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
Sodium Azide
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for c-jun [p Ser63] Antibody (1018Y) [Unconjugated]

  • Activator protein 1
  • AP1
  • AP-1
  • cJun
  • c-Jun
  • enhancer-binding protein AP1
  • Jun activation domain binding protein
  • jun oncogene
  • jun proto-oncogene
  • JUN
  • Proto-oncogene c-Jun
  • transcription factor AP-1
  • V-jun avian sarcoma virus 17 oncogene homologp39
  • v-jun sarcoma virus 17 oncogene homolog (avian)
  • v-jun sarcoma virus 17 oncogene homolog

Background

c-Jun is one of three Jun family proteins making up the Activator Protein-1 (AP-1) transcription factor group. c-Jun acts as a positive regulator of cell proliferation and can induce oncogenic transformation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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c-jun [p Ser63]
Antibody (1018Y) [Unc ...
MAB8930
c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Species: Hu
Applications: WB, Simple Western, ICC/IF

Publications for c-jun Antibody (MAB8930)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC, Western Blot.


Filter By Application
ICC
(1)
Western Blot
(1)
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Human
(2)
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Review for c-jun Antibody (MAB8930) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB8930:
Filter by Applications
Indirect Immunofluorescence
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Felix Oppel
 Indirect Immunofluorescence Human 05/03/2022
View

Summary

ApplicationOther
Sample TestedCell culture
SpeciesHuman
LotCJON0121051

Product General Protocols

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FAQs for c-jun Antibody (MAB8930) (0)

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Other c-jun MAB8930
Enlarge
 Felix Oppel
05/03/2022
Application: Indirect Immunofluorescence
Species: Human
read this review

Bioinformatics

Gene Symbol JUN
Uniprot