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PP2A alpha [p Tyr307] Antibody [Unconjugated]

 (11 Publications)
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Western blot shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with hydroxyurea. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3989), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PP2A (Y307), Catalytic Subunit at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with hydroxyurea. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3989-SP
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PP2A alpha [p Tyr307] Antibody [Unconjugated] Summary

Immunogen
Phosphopeptide containing human PP2A Y307 site
Modification
p Tyr307
Specificity
Detects human, mouse, and rat PP2A when phosphorylated at Y307. The antigen is identical to the PP4 Y305 phosphorylation site.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPP2CA
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF3989 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PP2A alpha [p Tyr307] Antibody [Unconjugated]

  • EC 3.1.3.16
  • PP2A
  • PP2A-alpha
  • PP2Ac
  • PP2CA
  • PP2Calpha
  • PPP2CA
  • protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
  • protein phosphatase 2, catalytic subunit, alpha isozyme
  • protein phosphatase 2A catalytic subunit, alpha isoform
  • Replication protein C
  • RP-C
  • serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform
  • serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Background

Protein Phosphatase 2A (PP2A) dephosphorylates serine and threonine residues in proteins. This ubiquitously expressed enzyme plays a critical role in modulating cell survival, growth factor responses, and neurotransmission. Phosphorylation near the C-terminus at Y307 of the catalytic subunit decreases the phosphatase activity of PP2A and reduces its ability to bind to proteins such as CD28 and the glutamate receptor GluR5. Phosphorylation of PP2A at Y307 is highest during M-phase and lowest during S-phase of the cell cycle. Transient PP2A phosphorylation at this site is also observed in growth factor-stimulated cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PP2A alpha Antibody (AF3989)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Neutralization, Western Blot.


Filter By Application
Neutralization
(2)
Western Blot
(5)
All Applications
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Human
(6)
Mouse
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF3989 Applications Species
Agus Suryawan, Marko Rudar, Jane K. Naberhuis, Marta L. Fiorotto, Teresa A. Davis Preterm birth alters the feeding-induced activation of Akt signaling in the muscle of neonatal piglets Pediatric Research 6/1/2023 [PMID: 36402914]
Y Chang, Y Yao, R Ma, Z Wang, J Hu, Y Wu, X Jiang, L Li, G Li Dl-3-n-Butylphthalide Reduces Cognitive Deficits and Alleviates Neuropathology in P301S Tau Transgenic Mice Frontiers in Neuroscience, 2021-02-10;15(0):620176. 2021-02-10 [PMID: 33642981] (Western Blot, Mouse) Western Blot Mouse
Yingying Qiao, Yuan Zhou, Chao Song, Xin Zhang, Yi Zou MID1 and MID2 regulate cell migration and epithelial-mesenchymal transition via modulating Wnt/ beta -catenin signaling Annals of Translational Medicine 2020-09-21 [PMID: 32953821]
IE Frohner, I Mudrak, S Schüchner, D Anrather, M Hartl, JM Sontag, E Sontag, BE Wadzinski, T Preglej, W Ellmeier, E Ogris PP2AC Phospho-Tyr307 Antibodies Are Not Specific for this Modification but Are Sensitive to Other PP2AC Modifications Including Leu309 Methylation Cell Rep, 2020-03-03;30(9):3171-3182.e6. 2020-03-03 [PMID: 32130916] (Neutralization, Human) Neutralization Human
S Mazhar, D Leonard, A Sosa, D Schlatzer, D Thomas, G Narla Challenges and Reinterpretation of Antibody-Based Research on Phosphorylation of Tyr307 on PP2Ac Cell Rep, 2020-03-03;30(9):3164-3170.e3. 2020-03-03 [PMID: 32130915] (Neutralization, Human) Neutralization Human
H Kawai, H Matsushita, R Suzuki, Y Kitamura, Y Ogawa, H Kawada, K Ando Overcoming Tyrosine Kinase Inhibitor Resistance in Transformed Cell Harboring SEPT9-ABL1 Chimeric Fusion Protein Neoplasia, 2019-07-03;21(8):788-801. 2019-07-03 [PMID: 31276931] (Western Blot, Human) Western Blot Human
Ruth Halaban, Antonella Bacchiocchi, Robert Straub, Jian Cao, Mario Sznol, Deepak Narayan et al. A novel anti-melanoma SRC-family kinase inhibitor Oncotarget 2019-03-19 [PMID: 31040916]
Lucas Sagrillo-Fagundes, Josianne Bienvenue-Pariseault, Cathy Vaillancourt Melatonin: The smart molecule that differentially modulates autophagy in tumor and normal placental cells PLOS ONE 2019-01-10 [PMID: 30629581]
P Liu, X Chen, H Zhou, L Wang, Z Zhang, X Ren, F Zhu, Y Guo, X Huang, J Liu, PS Spencer, X Yang The Isoquinoline Alkaloid Dauricine Targets Multiple Molecular Pathways to Ameliorate Alzheimer-Like Pathological Changes In Vitro Oxid Med Cell Longev, 2018-07-02;2018(0):2025914. 2018-07-02 [PMID: 30057671] (Western Blot, Human) Western Blot Human
X Huang, J Wang, X Chen, P Liu, S Wang, F Song, Z Zhang, F Zhu, X Huang, J Liu, G Song, PS Spencer, X Yang The Prenylflavonoid Xanthohumol Reduces Alzheimer-Like Changes and Modulates Multiple Pathogenic Molecular Pathways in the Neuro2a/APPswe Cell Model of AD Front Pharmacol, 2018-04-04;9(0):199. 2018-04-04 [PMID: 29670521] (Western Blot, Human) Western Blot Human
Show All 11 Publications.

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Bioinformatics

Gene Symbol PPP2CA