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Adenosylhomocysteinase/AHCY Antibody

Images

 
Orthogonal Strategies: Western Blot: Adenosylhomocysteinase/AHCY Antibody [NBP2-48817] - Analysis in human cell line CACO-2 and human cell line HeLa.
Immunocytochemistry/ Immunofluorescence: Adenosylhomocysteinase/AHCY Antibody [NBP2-48817] - Staining of human cell line CACO-2 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Adenosylhomocysteinase/AHCY Antibody [NBP2-48817] - Staining of human Small intestine shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Adenosylhomocysteinase/AHCY Antibody [NBP2-48817] - Staining of human Kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: Adenosylhomocysteinase/AHCY Antibody [NBP2-48817] - Staining of human Liver shows moderate cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: Adenosylhomocysteinase/AHCY Antibody [NBP2-48817] - Staining of human Testis shows strong nuclear and cytoplasmic positivity in spermatogonia.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Adenosylhomocysteinase/AHCY Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: HPSFVMSNSFTNQVMAQIELWTHPDKYPVGVHFLPKKLDEAVAEAHLGKLNVKLTKLTEKQAQYLGMSCDGPFKPDHY
Predicted Species
Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AHCY
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Adenosylhomocysteinase/AHCY Recombinant Protein Antigen (NBP2-48817PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Adenosylhomocysteinase/AHCY Antibody

  • Adenosylhomocysteinase
  • AdoHcyase
  • AHCY
  • EC 3.3.1.1
  • S-adenosylhomocysteine hydrolase
  • S-adenosyl-L-homocysteine hydrolase
  • SAHH
  • SAHHadoHcyase

Background

S-adenosylhomocysteine hydrolase catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AHCY