![Western Blot: SGLT1/SLC5A1 Antibody [NBP2-20338] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SGLT1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SGLT1-SLC5A1-Antibody-Western-Blot-NBP2-20338-img0007.jpg "Western Blot: SGLT1/SLC5A1 Antibody [NBP2-20338] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SGLT1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Ca, Rb, ShSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human SGLT1/SLC5A1. The exact sequence is proprietary. |
| Localization | Membrane; Multi-pass membrane protein |
| Predicted Species | Canine (90%), Sheep (90%), Rabbit (90%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SLC5A1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 73 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
| Publications using NBP2-20338 | Applications | Species |
|---|---|---|
| Yang S, Yang G, Wang X et al. SIRT2 alleviated renal fibrosis by deacetylating SMAD2 and SMAD3 in renal tubular epithelial cells Cell death & disease 2023-09-30 [PMID: 37777567] (IF/ICC, Human) | IF/ICC | Human |
| Herat LY, Matthews JR, Hibbs M et al. SGLT1/2 inhibition improves glycemic control and multi-organ protection in type 1 diabetes iScience 2023-08-18 [PMID: 37520739] (B/N, IHC, ELISA) | B/N, IHC, ELISA | |
| He P, Haque A, Lin S et al. Inhibition of autotaxin alleviates inflammation and increases the expression of sodium-dependent glucose cotransporter 1 and Na+/H+ exchanger 3 in SAMP1/Fc mice Am. J. Physiol. Gastrointest. 2018-08-17 [PMID: 30118349] (WB, Mouse) | WB | Mouse |
| Yang G, Wang X, Xiang J et al. SIRT2 Alleviated Renal Fibrosis by Deacetylating Conserved Lysine 451 on SMAD2 in Renal Tubular Epithelial Cells SSRN Electronic Journal 2022-07-28 |
Secondary Antibodies |
Isotype Controls |
Research Areas for SGLT1/SLC5A1 Antibody (NBP2-20338)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: SGLT1/SLC5A1 Antibody [NBP2-20338] - Analysis of methanol-fixed A431, using SGLT1antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/SGLT1-SLC5A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20338-img0004.jpg "Immunocytochemistry/Immunofluorescence: SGLT1/SLC5A1 Antibody [NBP2-20338] - Analysis of methanol-fixed A431, using SGLT1antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: SGLT1/SLC5A1 Antibody [NBP2-20338] - Human lung fibrose tissue, using SGLT1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SGLT1-SLC5A1-Antibody-Immunohistochemistry-Paraffin-NBP2-20338-img0005.jpg "Immunohistochemistry-Paraffin: SGLT1/SLC5A1 Antibody [NBP2-20338] - Human lung fibrose tissue, using SGLT1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: SGLT1/SLC5A1 Antibody [NBP2-20338] -Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SGLT1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/antibody/nbp2-20338_rabbit-polyclonal-sglt1-slc5a1-antibody-western-blot-692023114127..jpg "Western Blot: SGLT1/SLC5A1 Antibody [NBP2-20338] -Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SGLT1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")