![Western Blot: Glut5 Antibody (OTI9F3) [NBP1-47980] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti- GLUT5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Glut5-Antibody-OTI9F3-Western-Blot-NBP1-47980-img0018.jpg "Western Blot: Glut5 Antibody (OTI9F3) [NBP1-47980] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti- GLUT5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
| Reactivity | Hu, PmSpecies Glossary |
| Applications | WB, Flow, IHC, IP |
| Clone | OTI9F3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human SLC2A5 (NP_003030) produced in HEK293T cell. |
| Specificity | This antibody is specific for Homo sapiens solute carrier family 2 (facilitated glucose/fructose transporter), member 5 (SLC2A5), transcript variant 1. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SLC2A5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 54.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1% BSA, 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
IHC-P | Human | 12/06/2021 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Glut5 Antibody (OTI9F3) [NBP1-47980] - Human adult kidney tissue section stained with Glut5 antibody at 1:200. Secondary antibody at 1:300. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Glut5-Antibody-OTI9F3-Immunohistochemistry-Paraffin-NBP1-47980-img0024.jpg "Immunohistochemistry-Paraffin: Glut5 Antibody (OTI9F3) [NBP1-47980] - Human adult kidney tissue section stained with Glut5 antibody at 1:200. Secondary antibody at 1:300. IHC-P image submitted by a verified customer review.")
![Flow Cytometry: Glut5 Antibody (OTI9F3) [NBP1-47980] - Analysis of Jurkat cells, using anti-GLUT5 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Glut5-Antibody-OTI9F3-Flow-Cytometry-NBP1-47980-img0012.jpg "Flow Cytometry: Glut5 Antibody (OTI9F3) [NBP1-47980] - Analysis of Jurkat cells, using anti-GLUT5 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Western Blot: Glut5 Antibody (OTI9F3) [NBP1-47980] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLUT5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti- GLUT5.](http://images.novusbio.com/fullsize/Glut5-Antibody-OTI9F3-Western-Blot-NBP1-47980-img0017.jpg "Western Blot: Glut5 Antibody (OTI9F3) [NBP1-47980] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLUT5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti- GLUT5.")
![Immunohistochemistry-Paraffin: Glut5 Antibody (OTI9F3) [NBP1-47980] - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Glut5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP1-47980) Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/Glut5-Antibody-OTI9F3-Immunohistochemistry-Paraffin-NBP1-47980-img0021.jpg "Immunohistochemistry-Paraffin: Glut5 Antibody (OTI9F3) [NBP1-47980] - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Glut5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP1-47980) Image using the Azide and BSA Free format of this antibody.")
![Immunohistochemistry-Paraffin: Glut5 Antibody (OTI9F3) [NBP1-47980] - Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-Glut5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP1-47980) Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/Glut5-Antibody-OTI9F3-Immunohistochemistry-Paraffin-NBP1-47980-img0022.jpg "Immunohistochemistry-Paraffin: Glut5 Antibody (OTI9F3) [NBP1-47980] - Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-Glut5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP1-47980) Image using the Azide and BSA Free format of this antibody.")
![Immunohistochemistry-Paraffin: Glut5 Antibody (OTI9F3) [NBP1-47980] - Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-Glut5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP1-47980) Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/Glut5-Antibody-OTI9F3-Immunohistochemistry-Paraffin-NBP1-47980-img0023.jpg "Immunohistochemistry-Paraffin: Glut5 Antibody (OTI9F3) [NBP1-47980] - Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-Glut5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP1-47980) Image using the Azide and BSA Free format of this antibody.")
![Flow Cytometry: Glut5 Antibody (OTI9F3) [NBP1-47980] - Analysis of Hela cells, using anti-GLUT5 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Glut5-Antibody-OTI9F3-Flow-Cytometry-NBP1-47980-img0010.jpg "Flow Cytometry: Glut5 Antibody (OTI9F3) [NBP1-47980] - Analysis of Hela cells, using anti-GLUT5 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Immunoprecipitation: Glut5 Antibody (OTI9F3) [NBP1-47980] - Immunoprecipitation(IP) of Glut5 by using monoclonal anti-Glut5 antibodies (Negative control: IP without adding anti-Glut5 antibody). For each experiment, 500ul of DDK tagged Glut5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-Glut5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody. Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/Glut5-Antibody-OTI9F3-Immunoprecipitation-NBP1-47980-img0020.jpg "Immunoprecipitation: Glut5 Antibody (OTI9F3) [NBP1-47980] - Immunoprecipitation(IP) of Glut5 by using monoclonal anti-Glut5 antibodies (Negative control: IP without adding anti-Glut5 antibody). For each experiment, 500ul of DDK tagged Glut5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-Glut5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody. Image using the Azide and BSA Free format of this antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
